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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Pharmaciana: Jurnal Kefarmasian
Jo Suherman
Fakultas Kedokteran Universitas Kristen Maranatha, Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Anti-hyperlipidemic effect of Indonesian mangosteen peel extract in dyslipidemia-induced rats Darsono, Lusiana; Suherman, Jo; Widowati, Wahyu; Kusuma, Hanna Sari Widya
Pharmaciana Vol. 13 No. 3 (2023): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v13i3.26255

Abstract

Dyslipidemia, is due to an increase in blood lipid levels, which include cholesterol, triglycerides and low-density lipoprotein. Dyslipidemia is expected to remain as a major risk factor for cardiovascular diseases, diabetic and atherosclerosis. Mangosteen is an antioxidant agent that can exhibit a potential free radical scavenging property and protected oxidation of low-density lipoprotein. The aim of this research was to determine hypolipidemic and antioxidant effect of mangosteen peel extract (MPE) in dyslipidemia rats. Lipid profile including cholesterol total (CHOL), triglyceride (TG), High Density Lipoprotein (HDL) and Low-Density Lipoprotein (LDL) of dyslipidemia rats were measured using photometric method. Rats were fed cholesterol for 4 weeks until rats were indicated dyslipidemia. After rats suffered dyslipidemia, the high cholesterol feed was stopped and rats were given mangosteen peel extract 1000; 500; 250 mg/Kg body weight (bw) daily for 14 days (first treatment) and 28 days (second treatment), negative control (normal feed), and positive control (dyslipidemia rats). MDA plasma level also was measured. The CHOL, TG, LDL and HDL of dyslipidemia rats remarkable decreased after treated by 1000 mg/Kg mangosteen peel extract. After 28 days of treatment, the CHOL, TG and LDL were critically declined by 1000 mg/Kg mangosteen peel extract. MDA plasma level showed decreased in all treatments after 28 days of treatment. The dyslipidemia rats treated by mangosteen peel extract showed hypolipidemic activity, according to decreased level of lipid profile including cholesterol, triglycerides and LDL. The MDA level can also be decreased by mangosteen peel extract by increasing the concentration.
REGULATION OF ADIPOGENESIS AND KEY ADIPOGENIC GENE EXPRESSION BY MANGOSTEEN PERICARP EXTRACT AND XANTHONES IN 3T3-L1 CELLS Widowati, Wahyu; Darsono, Lusiana; Suherman, Jo; Afifah, Ervi; Rizal, Rizal; Arinta, Yukko; Mozef, Tjandrawati; Suciati, Tri
BIOTROPIA Vol. 27 No. 1 (2020): BIOTROPIA Vol. 27 No. 1 April 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.545 KB) | DOI: 10.11598/btb.2020.27.1.932

Abstract

Obesity is one of the risk factors for atherosclerosis, and its occurrence and development are associated with fat accumulation and adipocyte differentiation. Thus, the suppression of adipocyte differentiation can be a potential anti-obesity approach. This study examined the effect of mangosteen pericarp extract (MPE) and xanthones (α-Mangostin (AM) and γ-Mangostin (GM)) on the expression of PPARγ, C/EBPα, SCD1, LPL, aP2, adipoQ, and FAS in 3T3-L1 cells. Concentrations of MPE and xanthones used were based on cytotoxicity assays on 3T3-L1 cells. Three different MPE concentrations (0, 25, and 50 µg/mL), three AM concentrations (0, 25, and 50 µM), and GM concentrations (0, 50, and 75 µM) were used. The expressions of PPARγ, C/EBPα, SCD1, LPL, aP2, adipoQ, and FAS genes were measured using real-time quantitative PCR. Gene expression was downregulated in cells treated with 50 µg/mL MPE and 50 µM GM. However, 25 µM and 50 µM AM did not suppress PPARγ and SCD1 expression. The 50 µM AM treatment also failed to reduce aP2 gene expression. Overall, MPE and GM demonstrated potential anti-adipogenesis and anti-obesity effects by suppressing the expression of PPARγ, C/EBPα, SCD1, LPL, aP2, adipoQ, and FAS in 3T3-L1 cells.
Co-Authors Afifah, Ervi Arinta, Yukko Hanna Sari Widya Kusuma, Hanna Sari Widya Lusiana Darsono Rizal Rizal Tjandrawati Mozef Tri Suciati WAHYU WIDOWATI