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All Journal HAYATI Journal of Biosciences e-GIGI Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung BIOTROPIA - The Southeast Asian Journal of Tropical Biology Pharmaciana: Jurnal Kefarmasian Zenit Indonesian Journal of Cancer Chemoprevention Journal of Mathematical and Fundamental Sciences Folia Medica Indonesiana Global Medical and Health Communication Majalah Obat Tradisional Majalah Kedokteran Science and Technology Indonesia Jurnal Kedokteran Gigi Universitas Padjadjaran Molecular and Cellular Biomedical Sciences (MCBS) Journal Of Vocational Health Studies JFIOnline Journal Syifa Sciences and Clinical Research (JSSCR) Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Pharmaceutical Sciences and Research (PSR) Makara Journal of Science Cakradonya Dental Journal International Journal of Electrical, Computer, and Biomedical Engineering (IJECBE)
WAHYU WIDOWATI
Maranatha Christian University Bandung
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Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line Widowati, Wahyu; Mozef, Tjandrawati; Risdian, Chandra; Ratnawati, Hana; Tjahyani, Susy; Sandra, Ferry
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

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Abstract

Tapak dara or Madagascar Periwinkle (Catharanthus roseus [L] G.Don), a natural plant, is empirically reported to have promising anticancer activity. To elucidate its mechanism, a research was conducted to investigate the possible ethanol extract of  C.  roseus in inducing apoptosis on breast cancer cell line (T47D). Antioxidant activity of C. roseus was investigated as well. Sub-G1 flowcytometric apoptotic analysis result showed that extract of C. roseus at 6.25 μg/mL induced apoptosis for 26.365%. Increasing extract concentration resulted an increasing apoptotic level as well, extract at concentration of 12.5 μg/mL induced apoptosis for 22.235%.  Meanwhile doxorubicin at concentration of 10  μg/mL induced apoptosis for 36.055%. The antioxidant activity was determined by using  in vitro assay: inhibition of  2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging  activity. Antioxidant activity of  C. roseus extract were compared to quercetin and butylated hydroxyanisol (BHA), as positive controls.  The results showed that DPPH IC50 of C. roseus extract, quercetin and BHA were 358.411 μg/mL, 19.200 μg/mL  and  94.178  μg/mL, respectively. We suggest that  C. roseus extract had a potential anticancer activity by inducing apoptosis.
Free Radical Scavenging Activity of Ethanolic Leaves Extract and Its Different Solvent Fractions of Piper betle L. In Vitro Risdian, Chandra; Widowati, Wahyu; Mozef, Tjandrawati; Wargaseti, Teresa Liliana; Khiong, Khie
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Various diseases including cancer and cardiovascular diseasesare induced by free radicals via lipid peroxidation, protein peroxidation, DNA damage, and cellular degeneration in thecells.Piper betle L.,commonly called betel,belongs to thePiperaceae family.It originated from South and South Eas tAsia,and has been used as a traditional medicine by people theredue to its medicinal properties.In the present study, free radicalscavenging activity of ethanolic leaves extract of Piper betle L.together with its different solvent fractions(hexane,ethylacetate,butanol,and water)were evaluated by DPPH freeradical scavenging assay.The ethanolic extract and the ethylacetate fraction had shown very significant DPPH (1,1)diphenyl)2)picryl)hydrazyl) radical scavenging activitycompared to the other fractions.In DPPH free radical scavengingassay,the IC50 value of the ethanolic extract and the ethyl acetate fraction were found to be 17.431g/mL and 11.53 1g/mL, respectively. The free radical scavenging activity was assumed due to the total phenolic content.The total phenoliccontent was found to be highest in the ethanol extract(277.68µgEGCG/mg)and the ethyl acetate fraction(559.38µgEGCG/mg).The results concluded that the ethanolic leaves extract of Piper  betle L. and its ethyl acetate fraction have a consider ably freeradical scavenging activity.
Sel Punca sebagai Transformasi Alternatif Terapi Widowati, Wahyu; Widyanto, Rahma Micho
Zenit Vol 2, No 1 (2013)
Publisher : Zenit

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Abstract

Stem cells are cells that became the beginning of the growth  to others cell that constract the whole body of organisms, including human. Stem cells are from embryonic, fetal, or adult cells, which are capable to proliferate to cells in a long time, which this cell doesn’t have specific function, stem cells are able to differentiate  into specific cell that build tissues and organ systems. Stem cells have charateristic including :  undifferentiated cells, this cell is self-renewal, cells can differentiate into more than one type of cells (multipotent/pluripotent). Types of stem cells include embryonic stem cells derived from embryo, or inner cell mass (ICM) and adult stem cells found in bone marrow, cord blood, umbilical cord, peripheral blood, liver, skin, brain, dental pulp, fatty tissue. The uniqueness of stem cells give new hope as an alternative therapy or cells therapy for various types especially degenerative diseases. Keywords: stem cell, cell therapy
α-/β-Glucosidase and α-Amylase Inhibitory Activities of Roselle (Hibiscus sabdariffa L.) Ethanol Extract Gondokesumo, Marisca Evalina; Kusuma, Hanna Sari Widya; Widowati, Wahyu
Molecular and Cellular Biomedical Sciences Vol 1, No 1 (2017)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v1i1.3

Abstract

Background: Diabetes mellitus is a metabolic disease, characterized by hyperglycemia due to disturbance in both insulin secretion and function. One of theurapeutic approaches is to reduce blood glucose levels by inhbiting α-/β-glucosidase and α-amylase involved in carbohydrate digestion. Thus, inhibition of these enzymes play important role in the treatment of diabetes mellitus. Roselle (Hibiscus sabdariffa L.) has been known to have several medicinal properties and potency as an antidiabetics agents. This reseacrh aimed to observe antidiabetic properties of roselle ethanol extract (REE) towards α-glucosidase, β-glucosidase and α-amylase.Materials and Methods: REE was done with maceration technique using diluent of 70% ethanol. Antidiabetic properties were measured by inhibitory activity of α-amylase, α-glucosidase and β-glucosidase.Results: REE was able to inhibit α-/β-glucosidase and α-amylase in the highest concentration with inhibition percentage of 72.68, 47.34 and 73.08% respectively, and were comparable with Acarbose of 81.49, 50.97, 73.08%. The median inhibitory concentration (IC50) of α-/β-glucosidase and α-amylase of REE were 15.81, 41.77, 18.09 μg/mL respectively, and Acarbose were 9.45, 22.57, 3.64 μg/mL respectively.Conclusions: REE inhibits α-/β-glucosidase and α-amylase.Keywords: Roselle, Acarbose, α-glucosidase, β-glucosidase, α-amylase, antidiabetic
Free Radical Scavenging and α-Glucosidase Inhibitor Activity of Ethanolic Extract of Mucuna pruriens L. Widowati, Wahyu; Ratnawati, Hana; Retnaningsih, Ch.; Lindayani, .; Rusdi, Udju D.; Winarno, Wahyu
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 5, No 3 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Biji kacang “koro benguk” atau velvet bean sudah sangat dikenal tetapi dasar ilmiah penggunaannya sebagai obat tradisional terutama sebagai antioksidan dan antidiabetik masih belum diketahui. Oleh sebab itu dalam penelitian ini dilakukan percobaan untuk mengevaluasi aktivitas antioksidan dan antidiabetik dari ekstrak biji koro benguk, serta fraksi heksana, etil asetat, butanol dan air menggunakan uji in vitro menggunakan DPPH (1,1-difenil-2-pikril-hidrazil) dengan pembanding positif kuersetin dan BHA. Hasil penelitian menunjukkan bahwa IC50 DPPH untuk ekstrak biji koro benguk, fraksi heksana, etil asetat, butanol dan air berturut-turut sebesar 22,17; 152,60; 113,03; 4,93; dan 9,14 μg/mL, sedangkan untuk kuersetin dan BHA masing masing sebesar  4,28 dan 22,587 μg/mL. Aktivitas antidiabetik ditentukan menggunakan metode penghambatan α-glukosidase dengan glucobay sebagai kontrol positif. Hasil penelitian menunjukkan bahwa IC50 antidiabetik untuk ekstrak biji koro benguk, fraksi heksana, etil asetat, butanol, air, dan glucobay berturut-turut sebesar 24,44; 19,55; 8,40; 80,98; 48.97; dan 16,23 μg/mL. ABSTRACT Velvet bean seeds are ubiquitous but the scientific basis for its medicinal use especially as antioxidant and antidiabetic remains unknown. Therefore, a study was designed to evaluate  antioxidant and antidiabetic activities of the  extract, hexane, ethyl acetate, butanol and water fractions of velvet bean seeds. In order to evaluate antioxidant activity of extract and fractions  using  quercetin,  butylated hydroxyanisole (BHA) as positive control and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity was determined. The results showed that IC50 of DPPH were 22.17; 152.60; 113.03; 4.93; 9.14 μg/mL respectevily;  quercetin 4.28 and BHA 22.587 μg/mL.  Antidiabetic activity was determined by using  of α-glucosidase inhibition assay of  extract, four fractions and glucobay as positive control. The results showed that antidiabetic IC50 were24.44; 19.55; 8.40; 80.98; 48.97μg/mL respectively and glucobay as positive control  16.23 μg/mL.
Free Radical Scavenging and α-Glucosidase Inhibitor Activity of Ethanolic Extract of Mucuna pruriens L. Widowati, Wahyu; Ratnawati, Hana; Retnaningsih, Ch.; Lindayani, .; Rusdi, Udju D.; Winarno, Wahyu
Jurnal Farmasi Indonesia Vol 5, No 3 (2011)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v5i3.46

Abstract

Biji kacang â??koro bengukâ? atau velvet bean sudah sangat dikenal tetapi dasar ilmiah penggunaannya sebagai obat tradisional terutama sebagai antioksidan dan antidiabetik masih belum diketahui. Oleh sebab itu dalam penelitian ini dilakukan percobaan untuk mengevaluasi aktivitas antioksidan dan antidiabetik dari ekstrak biji koro benguk, serta fraksi heksana, etil asetat, butanol dan air menggunakan uji in vitro menggunakan DPPH (1,1-difenil-2-pikril-hidrazil) dengan pembanding positif kuersetin dan BHA. Hasil penelitian menunjukkan bahwa IC50 DPPH untuk ekstrak biji koro benguk, fraksi heksana, etil asetat, butanol dan air berturut-turut sebesar 22,17; 152,60; 113,03; 4,93; dan 9,14 μg/mL, sedangkan untuk kuersetin dan BHA masing masing sebesar  4,28 dan 22,587 μg/mL. Aktivitas antidiabetik ditentukan menggunakan metode penghambatan α-glukosidase dengan glucobay sebagai kontrol positif. Hasil penelitian menunjukkan bahwa IC50 antidiabetik untuk ekstrak biji koro benguk, fraksi heksana, etil asetat, butanol, air, dan glucobay berturut-turut sebesar 24,44; 19,55; 8,40; 80,98; 48.97; dan 16,23 μg/mL. ABSTRACT Velvet bean seeds are ubiquitous but the scientific basis for its medicinal use especially as antioxidant and antidiabetic remains unknown. Therefore, a study was designed to evaluate  antioxidant and antidiabetic activities of the  extract, hexane, ethyl acetate, butanol and water fractions of velvet bean seeds. In order to evaluate antioxidant activity of extract and fractions  using  quercetin,  butylated hydroxyanisole (BHA) as positive control and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity was determined. The results showed that IC50 of DPPH were 22.17; 152.60; 113.03; 4.93; 9.14 μg/mL respectevily;  quercetin 4.28 and BHA 22.587 μg/mL.  Antidiabetic activity was determined by using  of α-glucosidase inhibition assay of  extract, four fractions and glucobay as positive control. The results showed that antidiabetic IC50 were24.44; 19.55; 8.40; 80.98; 48.97μg/mL respectively and glucobay as positive control  16.23 μg/mL.
Comparison of Antioxidant and Anti-hyaluronidase Potentials of Pineapple Core Extract (Ananas comosus (L.) Merr.) and Luteolin Jusri, Ridza; Widodo, Wahyu Setia; Widowati, Wahyu; Armansyah, Adek; Sormin, Delores Elisabeth; Fachrial, Edy; Lister, I Nyoman Ehrich
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n2.1629

Abstract

Skin aging processes are divided into chronological aging and premature aging. Premature aging is generally caused by free radicals, from both air pollution and photoaging. Natural compounds from plant extracts are among sources of antioxidants and anti-hyaluronidase which have the ability to prevent antiaging. One of the potential fruits related to antioxidant and antiaging activities is Anana scomosus. A. comosus has a number of phenolic compounds with biological activities. One of the main phenolic compounds in A. comosus is luteolin. The aim of this study was to evaluate the antioxidant and antiaging potentials of Pineapple Core Extract (PCE). This study was conducted at the Biomolecular and Biomedicine Research Center, Aretha Medika Utama from August to November 2018. Analysis of antioxidants from PCE and luteolin was carried out using H2O2 scavenging activity assay. The antiaging assay was carried out through inhibition of hyaluronidase enzyme, one of the important enzymes in the aging process. Luteolin had lower IC50 value of H2O2 scavenging activity of around 24.12±3.13 μg/ml, which was better than CPE with IC50 of 304.56±3.76μg/mL. The results of hyaluronidase inhibition activity assay showed that luteolin compound had a lower IC50 value of 67.38±3.99 μg/mL when compared to PCE with an IC50 value of 161.15±1.05 μg/mL. Hence, Luteolin has higher antioxidant and anti-hyaluronidase activities than PCEPerbandingan Aktivitas Antioksidan dan Antihialuronidase Ekstrak Bonggol Buah Nanas (EBN) dengan Senyawa LuteolinTerdapat dua jenis proses penuaan kulit, penuaan kronologis dan penuaan dini. Penuaan dini umumnya disebabkan oleh radikal bebas, baik dari polusi udara maupun photoaging. Penggunaan antioksidan dan antihialuronidase untuk mencegah proses penuaan telah banyak diketahui. Ananascomosus merupakan salah satu tanaman yang memiliki potensi sebagai antipenuaan dan antioksidan karena mengandung sejumlah senyawa fenolik yang memiliki berbagai aktivitas biologis. Salah satu senyawa fenolik utama dalam A. comosus adalah luteolin. Tujuan penelitian ini adalah untuk mengevaluasi potensi antioksidan dan anti-aging dari Ekstrak Bonggol Nanas (EBN) dan senyawa pembandingnya yaitu luteolin . Penelitian ini dilakukan di Biomolecular and Biomedicine Research Center, Aretha Medika Utama dari Agustus hingga November 2018. Pengujian antioksidan dari EBN dan luteolin  dilakukan menggunakan uji pemerangkapan H2O2. Uji antipenuaan dilakukan melalui uji penghambatan aktivitas hialuronidase, salah satu enzim penting dalam proses penuaan. Hasil penelitian menunjukkan bahwa Luteolin  memiliki nilai IC50 aktivitas pemerangkapan H2O2lebih rendah sekitar 24.12±3.13 μg/ml lebih baik daripada EBN dengan IC50 sebesar 304.56±3.76μg/mL. Hasil uji aktivitas penghambatan hialuronidase, senyawa luteolin  memiliki nilai IC50 lebih rendah, yaitu sebesar 67,38±3,99 μg/mL dibanding dengan EBN nilai IC50 161,15±1,05μg/mL. Hasil ini menunjukkan bahwa senyawa pembanding luteolin  memiliki aktivitas antioksidan dan antihialuronidase yang lebih tinggi daripada EBN. 
Antioxidant and Antityrosinase Activities of Ethanolic Pachyrhizuserosus Peel and Tuber Extract Siregar, Irma Dolsyeria; Kusuma, Hanna Sari Widya; Widowati, Wahyu; Marpaung, Hans Hotma; Ferdinand, Sahna; Fachrial, Edy; Lister, I Nyoman Ehrich
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.515 KB) | DOI: 10.15395/mkb.v51n2.1628

Abstract

Aging process is a physiological process in living organisms caused by, among others, free radicals. One of the free-radical-related aging problems is skin hyperpigmentation (excessive melanin) due to increasing tyrosinase enzyme activities. Natural compounds are widely used as antioxidant and antiaging agents. Bengkuang (Pachyrhizuserosus) is known as a source of various active compounds which can be used against free radicals to reduce the risk of skin aging  through  tyrosinase enzyme inhibition. This study was performed in September 2018 in Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung, Indonesia to examine the antioxidant and antityrosinase properties of Pachyrhizuserosus peel extract (PPE) and Pachyrhizuserosus  tuber extract (PTE).The extraction of PPE and PTE was performed using 70% ethanol by maceration method, followed by  phytochemical analysis using modified Farnsworth method. Antioxidant activities were measured through 2,2-Diphenyl-1-picrylhydrazil (DPPH) scavenging activities while antiaging assay were conducted through the  tyrosinase activity inhibition. In this study, PPE contained saponin, tannin, triterpenoid, and terpenoid while the PTE showed the presence of flavonoid, saponin, phenol, tannin, and alkaloid in phytochemical analysis. In the antioxidant assay, PPE presented a higher DPPH scavenging activities (IC50= 84.09 µg/mL) when compared to PTE (IC50= 98.30 µg/mL)(p<0.05). In antiaging assay, PPE showed a higher tyrosinase inhibitory activities when compared to PTE with =97.05µg/mL and 194.51µg/mL,respectively. It can be concluded that PPE has antioxidant and antiaging activities effective for preventing skin aging. Aktivitas Antioksidan dan Antitirosinase Ekstrak Etanol Kulit dan Daging PachyrhizuserosusProses penuaan merupakan suatu proses fisiologis yang terjadi pada makhluk hidup yang dapat disebabkan oleh radikal bebas. Hiperpigmentasi kuli tmerupakan salah satu masalah penuaan yang disebabkan oleh radikal bebas melalui peningkatan aktivitas enzim tirosinase. Bengkuang (Pachyrhizus erosus) diketahui mengandung berbagai senyawa aktif yang dapat menangkal radikal bebas serta mengurangi risiko penuaan kulit. Penelitian dilaksanakan pada September 2018 di Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung, Indonesia untuk menguji kemampuan aktivitas antioksidan dan antitirosinase pada ekstrak etanol kulit bengkuang (EEKB) dan ekstrak etanol daging bengkuang (EEDB). Pembuatan ekstrak kulit dan daging bengkuang dilakukan dengan cara mengekstraksi bahan dengan etanol 70% menggunakan metode maserasi, kemudian dilanjutkan dengan analisis fitokimia ekstrak dengan modifikasi metode Farnsworth. Aktivitas antioksidan diuji dengan mengukur pemerangkapan 2,2-Diphenyl-1-picrylhydrazil (DPPH) sedangkan antiaging diuji dengan mengukur aktivitas penghambatan tirosinase. Pada uji fitokimia menunjukkan EEKB memiliki kandungan senyawa saponin, tanin, triterpenoid dan terpenoid, sedangkan EEDB menunjukkan kandungan senyawa flavonoid, saponin, fenol, tanin, dan alkaloid. Pada uji antioksidan, EEKB memiliki aktivitas tertinggi pada pemerangkapan DPPH (IC50=84.09 µg/mL) dibanding dengan EEDB (IC50=98.30 µg/mL) (p≤0.05). Pada pengujian antiaging, EEKB memiliki nilai yang lebih tinggi pada aktivitas penghambatan tirosinase dibandingkan dengan EEDB (IC50=97.05 µg/mL; 194.51 µg/mL (p≤0.05). Simpulan, EEKB memiliki aktivitas antioksidan dan antiaging sehingga efektif dalam mencegah penuaan kulit. 
Comparison of Antioxidant and Anti-Tyrosinase Activities of Pineapple (Ananas comosus) Core Extract and Luteolin Compound Vrianty, Dela; Qodariah, Rismawati Laila; Widowati, Wahyu; Sinaga, Ade Putra Fratama; Fibrina, Dewi; Fachrial, Edy; Lister, I Nyoman Ehrich
Jurnal Kedokteran Brawijaya Vol 30, No 4 (2019)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.679 KB) | DOI: 10.21776/ub.jkb.2019.030.04.2

Abstract

Free radicals and UV exposure can cause aging. Aging prevention needs substances that can prevent molecular oxidation reactions in cells and inhibit the activity of enzymes that trigger aging. Research on pineapple skin and flesh extract (Ananas comosus (L.) Merr.) reported the presence of luteolin compound which functions as antioxidants and antityrosinase. However, in this study, the object used was pineapple core extract (PCE), which has not been widely known for its antioxidant and antityrosinase activity. Therefore, the purpose of this study was to determine the content of phytochemical compounds, antioxidant activity, and inhibitory activity of tyrosinase enzymes by PCE and then compared with the luteolin (LT) compound test results using Fansworth method, DPPH scavenging activity assay and tyrosinase enzyme inhibition assay. Based on the study results, the phytochemical compounds contained in PCE were tannins and triterpenoids.  PCE and LT produced the highest DPPH scavenging activity, which was 64.86% and 59.32% (final concentration 200μg/ml and 6.25μg/ml) and the highest tyrosinase inhibition activity, which was 60.52% and 85.02% (final concentration 100 μg/ml). Antioxidant activity was determined based on IC50 of 87.46µg/mL and 4.17 µg/ml respectively. IC50 tyrosinase enzyme inhibition EBN and LT respectively at 62.27µg/ml and 5.25 µg/ml. Antioxidant activity through DPPH free radical scavenging test and tyrosinase enzyme inhibition activity by PCE was lower than LT.
Antioxidant and Anti-Hyaluronidase Activities of Dragon Fruit Peel Extract and Kaempferol-3-O-Rutinoside Liana, Liana; Rizal, Rizal; Widowati, Wahyu; Fioni, Fioni; Akbar, Khainir; Fachrial, Edy; Lister, I Nyoman Ehrich
Jurnal Kedokteran Brawijaya Vol 30, No 4 (2019)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.286 KB) | DOI: 10.21776/ub.jkb.2019.030.04.3

Abstract

Aging is a natural process in human life and is triggered by the presence of free radicals (ROS). The use of antioxidants from natural ingredients is one of the breakthroughs to overcome aging and counteract the harmful effects caused by the free radicals. This study aimed to determine and compare the antioxidant activity of H2O2 scavenging and hyaluronidase inhibition of red dragon fruit peel extract (DFPE) and kaempferol-3-o-rutinoside (KOR) compounds. Dragon fruit peel extract (DFPE) is obtained through extraction by maceration method using 70% ethanol solvent. The design of this study included antioxidant and anti-aging activity assay of EKBN and KOR at the series concentration of 15.63; 31.25; 62.50; 125; 250; 500 µg/mL through H2O2 scavenging, as well as the DFPE and KOR hyaluronidase inhibition assay at the series concentration of 5.21; 10.42; 20.83; 41.7; 83.33; 166.67 µg/mL. EKBN shows that the average activity of H2O2 scavenging is lower than KOR. In addition, the IC50 values of KOR for H2O2 scavenging is lower (351.46±2.30ug/mL) than DFPE (409.64±23.17ug/mL). While, KOR also has higher values of inhibitory activity than of the DFPE. However, the IC50 value of KOR for hyaluronidase inhibition activity was 84.07±10.46µg/mL, equivalent to the IC50 value of DFPE (85.32±10.24µg/mL). The presence of antioxidant and anti-aging activity in the EKBN is probably caused by betalain and the KOR compound itself contained in red dragon fruit. The results of the paired-samples T-test on antioxidant activity and anti-aging of DFPE and KOR showed non-significant difference. Thus, DFPE has an equivalent antioxidant and anti-aging through H2O2 scavenging and hyaluronidase activity as possessed by the KOR compound.
Co-Authors - Lindayani AA Sudharmawan, AA Afifah Bambang Sutjiatmo, Afifah Bambang Afifah, Ervi Agung Novianto, Agung Agus Susanto Ahmad Faried Alexandro, Garry Annaba, Aziz Arinta, Yukko Armansyah, Adek Arrahmani, Betharie Cendera Aryenti, Aryenti Ayuni, Vini Azis, Rizal Bangun, Adityana Arta Batubara, Lilian Ch. Retnaningsih, Ch. Chandra Risdian Chiuman, Linda Damayanti, Ndaru Andri Dani Dani Dewi Nurul, Dewi Dewi, Intan Keumala Didik Priyandoko Djaja Rusmana Edriayani, Novi Edwin Setiabudi Edy Fachrial Eka Yudha Rahman Elisabeth, Mariska Eltania, The Fransiska Elvira, Cut Endah Purnamasari Esti Mumpuni, Esti Euis Reni Yuslianti, Euis Fahrauk, M. Fanny Rahardja Fauziyah, Nida Firyal Ferdiansyah, Rival Ferdinand, Sahna Ferry Sandra Fibrina, Dewi Fioni, Fioni Geeta, Ginting, Chrismis Novalinda Girsang, Ermi Gosandra, Sherrina O. Gunadi, Michael Sebastian Hadiprasetyo, Dhanar Septyawan Halim, Nathanael HANA RATNAWATI Handayani, Rr. Anisa Siwianti Hanna Sari Widya Kusuma, Hanna Sari Widya Harjanti, Mathelda Weni Harry Murti Hartini Tiono Haryanti, Mathelda Weni Hernawati Hernawati Irfan Syarif, Irfan Irwan, Mulia Jeffrey ., Jeffrey Jo Suherman Johan Lucianus Joko Raharjo, Sentot Juansah, Rahadian Deden Julio Arif Jusri, Ridza Khainir Akbar, Khainir Khie Khiong Ksatrianto, Faris Kusdianti Kusdianti Liana Liana Lindayani, . Lister, I Nyoman Ehrich Lusiana Darsono MADE PHARMAWATI Malau, Reghita Claudia Manurung, Mian Maranata Br Marisca Evalina Gondokesumo, Marisca Evalina Marpaung, Hans Hotma Mawarni, Evi Mulyasari, Tira Endang Nafik, Said Nainggolan, Ita Margaretha Nasution, Ali Napiah Natadilandes, Reyhan Ngurah Intan Wiratmini Nia Kania Nindia Salsabila Mia Dewi Nindya, Faradhina Salfa Ningrum, Siti Ratu Rahayu Novilla, Arina Nunung Ainur Rahmah Nurjamil, Aris Muhammad Nursanti, Okta Obeng, Selonan Philips Onggowidjaja Pradipta, Regina Purwoko, Agus Qamarani, audina Qlintang, Sandy Qodariah, Rismawati Laila Qodriah, Rahmatul R. Sapto Hendri Boedi Soesatyo Rahma Micho Widyanto RAHMAT, DENI Rita Tjokropranoto Rizal Aziz, Rizal Rizal Rizal Sabrina, Adilah Hafizha Nur Sachrowardi, Qomariyah Romadhiyani Salfa Nindya, Faradhina Salsabila, Nindia Santiadi, Sherly Sarwono, Sylvie Saufa, Zahra Qisthi Sholihah, Ika Adhani Sijani Prahastuti Sinaga, Ade Putra Fratama Siregar, Irma Dolsyeria Solihat, Ananda Khairunisa Sormin, Delores Elisabeth Sosiawan, Insan Sri Utami Suci Nar Vikasari, Suci Nar Sugiaman, Vinna Kurniwati Suhantoa, Deviana Lavender Sukandar, Elin Y. Susi Endrini Susy Tjahyani Sutjiatmo, Afifah B Syamsi, Lusi Nursilawati Syamsudin Syamsudin Takasenserang, Oktaviana Teddy Marcus Zakaria Teresa Liliana Wargasetia Tjandrawati Mozef Tri Suciati Triharsiwi, Dwi Nur Udju D. Rusdi Utomo, Herry S Utoyo, Frederick Surya vera vera, vera Vinna K. Sugiaman Vinna Kurniawati Sugiaman, Vinna Kurniawati Vrianty, Dela Wahyu Surakusumah WAHYU WINARNO Wangguai, Vannia Wibowo, Satrio Haryo Benowo Widodo, Wahyu Setia Widya Kusuma, Hanna Sari Widya Kusuma Yuninda, Viranda Andria Yusuf, Lydia Zahira, Nurul Zahra Zahiroh, Fadhilah Haifa