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All Journal HAYATI Journal of Biosciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology Pharmaciana: Jurnal Kefarmasian Global Medical and Health Communication Padjadjaran Journal of Dentistry

Lusiana Darsono

Fakultas Kedokteran Universitas Kristen Maranatha

Author-ID : 250312

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Articles

Ekstrak Daun Sirih (Piper betle Linn.) dalam Mempercepat Penyembuhan Luka Pramana, Komang A.; Darsono, Lusiana; Evacuasiany, Endang; S, Slamet
Global Medical & Health Communication (GMHC) Vol 2, No 2 (2014)
Publisher : Fakultas Kedokteran Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstrak Luka merupakan sebuah cedera pada tubuh yang sering terjadi di dalam kehidupan sehari-hari. Berbagai usaha dilakukan untuk menyembuhkan luka, mulai dari mencuci luka, pemberian antiseptik, sampai menggunakan bagian dari tanaman. Tujuan penelitian ini untuk mengetahui apakah sediaan salep yang mengandung ekstrak etanol daun sirih (Piper betle Linn.) dapat mempercepat penyembuhan luka. Penelitian ini bersifat prospektif eksperimental uji praklinis sungguhan, menggunakan rancangan percobaan acak lengkap (RAL) dan komparatif. Hewan coba yang digunakan adalah 25 ekor mencit betina dewasa galur Swiss Webster. Kulit paha masing-masing mencit dicukur bulunya, lalu dibuat luka sayat sampai dermis sepanjang 8 mm. Mencit dibagi dalam 5 kelompok secara acak, kelompok pertama diberikan vaselin album secara topikal, kelompok kedua diberikan salep povidon iodin 10% secara topikal, kelompok ketiga diberikan salep ekstrak etanol daun sirih (EEDS I) 10% secara topikal, kelompok keempat diberikan salep (EEDS II) 20% secara topikal, dan kelompok kelima diberikan salep (EEDS III) 30% secara topikal. Data yang diukur adalah lama waktu penyembuhan luka sampai luka menutup sempurna dalam hari. Analisis data menggunakan metode one way analysis of variance (ANOVA) dilanjutkan dengan Tukey HSD dengan Î±=0,05. Diperoleh waktu rata-rata yang diperlukan (hari) oleh setiap kelompok untuk penyembuhan luka, pada kelompok I adalah 7,2; kelompok II 5,8; kelompok III 5,8; kelompok IV 5,6; dan kelompok V 6,2 (p=0,016). Berdasarkan Uji beda rata-rata Tukey HSD, didapatkan perbedaan bermakna antara kelompok I dan kelompok II (p=0,043), kelompok III (p=0,043), serta kelompok IV (p=0,017). Simpulan penelitian ini adalah sediaan salep yang mengandung ekstrak etanol daun sirih (Piper betle Linn.) dapat mempercepat penyembuhan luka pada mencit. Kata kunci: Ekstrak etanol daun sirih, penyembuhan luka, salepÂ Piper Betle Extract (Piper betle Linn.) to Improve Healing Process Abstract Wound is an injury at the body that usually happen in daily activity. Many kind of treatment is taken to heal the wound like washing and cleaning the wound, using antiseptic, and using part of the plant. Objective of this research was to understand the effect of Piper betle etanol extract ointment to improve the wound healing process. This research is an experimental, using randomized control design. The animals were 25 adults Swiss Webster mice. The hair on each mouseâs thigh was cut and a 8 mm incision was made on the skin. The mice randomly divided into five groups, the first group treated with vaseline album topically, the second group treated with povidone iodine 10% ointment topically, the third group were given Piper betle etanol extract 10% ointment, the fourth group were given Piper betle etanol extract 20% ointment, and the fifth group were given Piper betle etanol extract 30% ointment. The intervention and measurement were done every day until the wound heal perfectly. The results showed that the average time needed (days) to heal perfectly in 1st group was 7.2, 2nd group was 5.8, 3rd group was 5.8, 4th group was 5.6, and 5th group was 6.2 (p=0.016). The average difference using Tukey HSD showed significant difference between 1st group and 2nd group (p=0.043), 3rd group (p=0.043), and 4th group (p=0.017). It is concluded that the treatment with Piper betle etanol extract as ointment increases the velocity of wound healing on mice. Key words: Ointment, piper betle etanol extract, wound healing

Anti-inflammatory properties of mangosteen peel extract on the mice gingival inflammation healing process Putri, Khairani; Darsono, Lusiana; Mandalas, Henry
Padjadjaran Journal of Dentistry Vol 29, No 3 (2017): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Introduction: Mangosteen (Garcinia Mangostana L.) peel extract has widely used in the pharmaceutical field due to its anticancer, anti-inflammatory, antibacterial, and immunity boost properties. It had been proofed to be able to prevent and reduce the amount of plaque and cure gingivitis. This study was aimed to compare mangosteen peel extract at the concentration of 12.5 and 25% on the mouse gingival inflamÂmation healing process. Methods: This study was a true experimental laboratory study. The subjects consisted of 28 mice divided into four groups, which were negative control (Aquadest) group; positive control (0.2% of Chlorhexidine) group; 12.5% Group of mangosteen peel extract group; and 25% respecÂtively. Examination of the inflammatory healing process was observed every 2 hours during 6 hours, and the inflammatory measurements of mouse gingival performed by using calipers. Data obtained was anÂalyzed with the one-way ANOVA test (Î±=0.05) and the Tukeyâs range test. Results: The results from the one-way ANOVA test and the Tukeyâs range test found that there was a significant difference on the inÂflammation size between the group with 12.5% of mangosteen peel extract and the group with aquadest and 0.2% of chlorhexidine. Meanwhile, the mice group with 12.5% of mangosteen peel extract and group with 25% of mangosteen peel extract did not show a significant difference in inflammatory size decrease. Conclusion: The mangosteen peel extract at the concentration of 12.5% was showing the highest anti-inÂflammatory potentials since the first measurement on the second hour after treatment.

Potential of Clitoria ternatea L. Extract Towards Insulin Receptor Expression and Marker of Inflammation in Diabetes Mellitus Rats Model Rusmana, Djaja; Tiono, Hartini; Widowati, Wahyu; Lucianus, Johan; Darsono, Lusiana; Onggowidjaja, Philips; Tjokropranoto, Rita; Vera, Vera; Setiabudi, Edwin; Obeng, Selonan; Kusuma, Hanna Sari Widya; Novianto, Agung
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.803-810

Metabolic abnormalities caused by the accumulation of human, environmental, genetic and lifestyle variables can be found in diabetes mellitus (DM). An increase in blood glucose carried on by a reduction in insulin production can also result in DM. Insulin resistance often occurs as a result of obesity and a lack of physical activity and aging. Telang flower extract (Clitoria ternatea L.) is reported to have several qualities such as being able to treat DM, chronic bronchitis, goiter, mucosal disorders and leprosy. This study’s objective was to elucidate anti-dibetic effect of C. ternatea extract (CTE) in rats with diabetic complications related to dyslipidemia. Rats suffering from diabetes will be examined after being given oral doses of Simvastatin 0.9 mg/kg BW, Glibenclamide 0.45 mg/kg BW, and 200, 400, and 800 mg/kg BW CTE, model induced by streptozotocin. The expression of the insulin gene (INS-1) was investigated by qRTPCR, Tumor Necrosis Factor-α (TNF-α), and Interleukin-1β (IL-1β) pancreatic DM rats model using the Immunohistochemistry (IHC) test. Both descriptive and quantitative data were acquired for the data. After utilizing ANOVA to evaluate quantitative data, the Tukey post hoc test was used to analyze the data, the expression of TNF-α and IL-1β was found to decrease while INS-1 expression increased in response to CTE. This effect was attributed to the modulation of TNF-α, IL-1β, and INS-1 expression. These findings suggest that CTE possesses antidiabetic properties.

Introduction: Mangosteen (Garcinia Mangostana L.) peel extract has widely used in the pharmaceutical field due to its anticancer, anti-inflammatory, antibacterial, and immunity boost properties. It had been proofed to be able to prevent and reduce the amount of plaque and cure gingivitis. This study was aimed to compare mangosteen peel extract at the concentration of 12.5 and 25% on the mouse gingival inflammation healing process. Methods: This study was a true experimental laboratory study. The subjects consisted of 28 mice divided into four groups, which were negative control (Aquadest) group; positive control (0.2% of Chlorhexidine) group; 12.5% Group of mangosteen peel extract group; and 25% respectively. Examination of the inflammatory healing process was observed every 2 hours during 6 hours, and the inflammatory measurements of mouse gingival performed by using calipers. Data obtained was analyzed with the one-way ANOVA test (α=0.05) and the Tukey’s range test. Results: The results from the one-way ANOVA test and the Tukey’s range test found that there was a significant difference on the inflammation size between the group with 12.5% of mangosteen peel extract and the group with aquadest and 0.2% of chlorhexidine. Meanwhile, the mice group with 12.5% of mangosteen peel extract and group with 25% of mangosteen peel extract did not show a significant difference in inflammatory size decrease. Conclusion: The mangosteen peel extract at the concentration of 12.5% was showing the highest anti-inflammatory potentials since the first measurement on the second hour after treatment.

Anti-hyperlipidemic effect of Indonesian mangosteen peel extract in dyslipidemia-induced rats Darsono, Lusiana; Suherman, Jo; Widowati, Wahyu; Kusuma, Hanna Sari Widya
Pharmaciana Vol. 13 No. 3 (2023): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v13i3.26255

Dyslipidemia, is due to an increase in blood lipid levels, which include cholesterol, triglycerides and low-density lipoprotein. Dyslipidemia is expected to remain as a major risk factor for cardiovascular diseases, diabetic and atherosclerosis. Mangosteen is an antioxidant agent that can exhibit a potential free radical scavenging property and protected oxidation of low-density lipoprotein. The aim of this research was to determine hypolipidemic and antioxidant effect of mangosteen peel extract (MPE) in dyslipidemia rats. Lipid profile including cholesterol total (CHOL), triglyceride (TG), High Density Lipoprotein (HDL) and Low-Density Lipoprotein (LDL) of dyslipidemia rats were measured using photometric method. Rats were fed cholesterol for 4 weeks until rats were indicated dyslipidemia. After rats suffered dyslipidemia, the high cholesterol feed was stopped and rats were given mangosteen peel extract 1000; 500; 250 mg/Kg body weight (bw) daily for 14 days (first treatment) and 28 days (second treatment), negative control (normal feed), and positive control (dyslipidemia rats). MDA plasma level also was measured. The CHOL, TG, LDL and HDL of dyslipidemia rats remarkable decreased after treated by 1000 mg/Kg mangosteen peel extract. After 28 days of treatment, the CHOL, TG and LDL were critically declined by 1000 mg/Kg mangosteen peel extract. MDA plasma level showed decreased in all treatments after 28 days of treatment. The dyslipidemia rats treated by mangosteen peel extract showed hypolipidemic activity, according to decreased level of lipid profile including cholesterol, triglycerides and LDL. The MDA level can also be decreased by mangosteen peel extract by increasing the concentration.

Regulation of Adipogenesis and Key Adipogenic Gene Expression by Mangosteen Pericarp Extract and Xanthones in 3T3-L1 Cells Widowati, Wahyu; Darsono, Lusiana; Suherman, Jo; Afifah, Ervi; Rizal, Rizal; Arinta, Yukko; Mozef, Tjandrawati; Suciati, Tri
BIOTROPIA Vol. 27 No. 1 (2020): BIOTROPIA Vol. 27 No. 1 April 2020
Publisher : SEAMEO BIOTROP

Obesity is one of the risk factors for atherosclerosis, and its occurrence and development are associated with fat accumulation and adipocyte differentiation. Thus, the suppression of adipocyte differentiation can be a potential anti-obesity approach. This study examined the effect of mangosteen pericarp extract (MPE) and xanthones (α-Mangostin (AM) and γ-Mangostin (GM)) on the expression of PPARγ, C/EBPα, SCD1, LPL, aP2, adipoQ, and FAS in 3T3-L1 cells. Concentrations of MPE and xanthones used were based on cytotoxicity assays on 3T3-L1 cells. Three different MPE concentrations (0, 25, and 50 µg/mL), three AM concentrations (0, 25, and 50 µM), and GM concentrations (0, 50, and 75 µM) were used. The expressions of PPARγ, C/EBPα, SCD1, LPL, aP2, adipoQ, and FAS genes were measured using real-time quantitative PCR. Gene expression was downregulated in cells treated with 50 µg/mL MPE and 50 µM GM. However, 25 µM and 50 µM AM did not suppress PPARγ and SCD1 expression. The 50 µM AM treatment also failed to reduce aP2 gene expression. Overall, MPE and GM demonstrated potential anti-adipogenesis and anti-obesity effects by suppressing the expression of PPARγ, C/EBPα, SCD1, LPL, aP2, adipoQ, and FAS in 3T3-L1 cells.

Co-Authors Afifah, Ervi Agung Novianto, Agung Arinta, Yukko Djaja Rusmana Edwin Setiabudi Endang Evacuasiany Hanna Sari Widya Kusuma, Hanna Sari Widya Hartini Tiono Henry Mandalas, Henry Jo Suherman Johan Lucianus Khairani Putri, Khairani Komang A. Pramana, Komang A. Obeng, Selonan Philips Onggowidjaja Rita Tjokropranoto Rizal Rizal Slamet S, Slamet Tjandrawati Mozef Tri Suciati vera vera, vera WAHYU WIDOWATI

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