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Sulastri Isminingsih
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Agriculture, Biological Sciences & Forestry

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PERTUMBUHAN TANAMAN KANTONG SEMAR (Nepenthes rafflesiana Jack.) DENGAN MODIFIKASI KONSENTRASI MEDIA DAN pH SECARA IN VITRO Lea Yony Kunita; Susiyanti .; Sulastri Isminingsih; Yupi Isnaini
Jurnal Agroekoteknologi Vol 3, No 1 (2011)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.084 KB) | DOI: 10.33512/j.agrtek.v3i1.571

Abstract

This research about growth of pichter plant Nepenthes rafflesiana Jack was aimed to determine the modification medium’s concentration and pH at in vitro . This research has been conducted in the laboratory of plant concervation center Bogor Botanical Garden. This research was doing from Juni to September 2010. research using randomized block design with 2 factor, the first factor is modification concentration medium MS, consisting of 5 level is 1 MS, 1/2 MS, 1/4 MS, 1/8 MS, dan 1/16 MS; and the second factor is the pH medium was consisted 3 level: pH 4,7, pH 5,7 dan pH 6,7 was repeated four times. The results showed that concentration medium 1/8 MS and all pH medium were significant the variable of number pichter, number rootlet, percentage of planlet life, percentage of planlet pichter, percentage of planlet rootlet.Key words: Nepenthes rafflesiana Jack, medium concentration, pH.
DAYA REGENERASI PLBs ANGGREK DENDROBIUM VAR. JACQUELINE THOMAS x WALTER OUMAE DAN KUMALA AGRIHORTI PADA JENIS MEDIA KULTUR IN VITRO DENGAN PENAMBAHAN AIR KELAPA Sri Rianawati; Sulastri Isminingsih; Nuniek Herminta; Luqyana Ulfa Riyadi
Jurnal Agroekoteknologi Vol 13, No 2 (2021)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v13i2.13167

Abstract

Dendrobium is the epiphytic orchid and high demand because of its long-lasting freshness, color variants, shape, and high productivity. The addition of organic complex Coconut Water (CW) to the in vitro media such as MS and VW because in general organic complex compounds are sources of sugars, vitamins, growth regulators, and amino acids. The purpose of this study was to obtain the best media for plantlet regeneration from PLBs of Dendrobium orchids var. JTWO and var. Kumala Agrihorti. This study used a Completely Randomized Design consisting of two factors with five replications. The combination of  planting media with the addition of coconut water was consist of 10 levels, ½ MS+0 ml/l CW, ½ MS+75 ml/l CW, ½ MS+100 ml/l CW, ½ MS+125 ml/l CW, ½ MS+150 ml/l CW, VW+0 ml/l CW, VW+75 ml/l CW, VW+100 ml/l CW, VW+125 ml/l CW and VW+150 ml/l CW. The results showed that the variety treatment gave a significant effect on the percentage of PLBs regeneration, the number of shoots, and the increase in plantlet height. The treatment of media gave a significant effect on the percentage of PLBs regeneration, percentage of PLBs life, the number of leaves, and the number of roots. The interaction between treatments significantly affects the percentage of PLBs regeneration.
IDENTIFIKASI MORFOLOGI TANAMAN AREN ASAL KABUPATEN LEBAK Nurmayulis Nurmayulis; Susiyanti Susiyanti; Sulastri Isminingsih; Reza Maulana Muhammad; Saiful Saiful; Septi Yulianti; Rizky Puspita Sari
Jurnal Agroekoteknologi Vol 13, No 2 (2021)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v13i2.13159

Abstract

Sugar palm is a commodity that has the potential to be developed. Sugar palm is developed to produce various products and raw materials for derivative products. Currently, there is a lot of morphological diversity of sugar palm plants found in the field. Presently, identification of various types of sugar palm morphologically in Banten Province is still limited. Sugar palm in Banten Province is found in various locations, such as in Lebak. This study aimed to determine the characteristics of Aren seedlings originating from Lebak Regency. The hypothesis in this study is that there are several characteristics of palm seedlings originating from Lebak Regency. This research was conducted in August 2019 - November 2019 located in Green House, Cinanggung Village, Serang City, Banten Province. This study uses a descriptive method, through a quantitative approach with data analysis and interpretation, and conclusion. The observation parameters in this study were: Plant Height (cm); Number of leaves per plant (strands); Leaf width (cm); Leaf length (cm); stem diameter (cm); Leaf color. The results showed that Aren from Lebak Regency aged 1 year have an average plant height of 28.1 cm, a pinnate leaf shape and a thick like a palm leaf, with an average number of 1 leaves, an average leaf length of 16.4 cm, and an average leaf width of 6.8 cm, with dark green leaf color. The stem diameter has a strength correlation with plant growth level like as plant height, the higher the plant. The greater the stem diameter obtained in this study is 2.19 cm.
HOMOLOGI FUNGSI GEN KNAT1 ( Knotted 1– like Arabidopsis thaliana) PADA ANGGREK BULAN Phalaenopsis amabilis (L.) Bl. DENGAN MEDIATOR Agrobacterium tumefaciens Sulastri Isminingsih; Endang Semiarti; Aziz Purwantoro
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.491 KB) | DOI: 10.33512/j.agrtek.v1i1.558

Abstract

ABSTRACTTo understand the function of KNAT1 gene Arabidopsis in the forming and developing of Indonesian origin orchid shoot Phalaenopsis amabilis through transform p35S::KNAT1 and pGreen to protocorm like bodies (plb) of the orchid mediated by Agrobacterium tumafaciens LBA4404. The plb transformants were grown on New Phalaenopsis selection medium containing 5 mg/l BAP, 0.15 mg/l NAA, 15 mg.l Kanamycin and addition of 300 mg/l Cefotaxim to eliminate the overgrowth of Agrobacterium. The analysis of positive transformant use Polimerase Chain Reaction (PCR) with the specific oligonucleotide primers for KNAT1 gene: KNAT1F1R1 and universal primers for pGreen: 35SO and Tnos. The result shows that 3 shoots of 1850 transformants positively carry out the 35S::KNAT1 construct (frequency of transformation was 0.16 %) while 5 shoot of 1850 transformants also positively carry out the pGreen vector, with the frequency of transformation was 0.27 %. The phenotype analysis of 35S::KNAT1 transformants show multiplication on forming of the leaf from a plb to + 10 shoots and forming of the leaf shape which has terompet like shapes and rectangular shape.Key words: Arabidopsis, KNAT1 gene, Phalaenopsis amabilis, shoot
UJI KETAHANAN ENAM VARIETAS PADI (Oryza sativa L.) TERHADAP KEKERINGAN SECARA MORFOLOGI DAN MOLEKULER Susiyanti Susiyanti; Sulastri Isminingsih; Zahratul Millah; Widi Ayutami
Jurnal Agroekoteknologi Vol 14, No 1 (2022)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v14i1.15656

Abstract

Rice (Oryza sativa L.) is a staple crop that is always needed throughout the world, including Asia. Rice is one of the staple foods in Indonesia. One form of abiotic stress that becomes the main obstacle in rice production is drought stress. It is necessary to test rice varieties that are resistant to drought stress. This study aims to determine drought-resistant rice varieties based on SSR markers and to determine the morphological characteristics of drought-resistant rice. This research was carried out from January to April 2021 at the Laboratory of Physiology and Biotechnology, Faculty of Agriculture, Sultan Ageng Tirtayasa University and the Screen House of the Agricultural Technology Research Center Banten. The DNA isolation process in the molecular resistance test used the Doyle & Doyle (1990) method which used three SSR markers to detect drought resistance genes. Analysis of DNA bands using Gel Analyzer software, grouping analysis using the UPGMA method in NTSYS software. The results of the morphological study of six rice varieties on drought showed significant to very significant effects on the parameters of plant height (cm), root length (cm), number of tillers, and number of panicles. Molecular test results from three SSR primers on six rice varieties showed a high level of polymorphism with an average polymorphism information content (PIC) of 0.72 which was included in the very informative category and could be researched on drought resistance. The genetic similarity identified Jawara Hawara rice variety have drought resistance genes.
Co-Authors Aziz Purwantoro Endang Semiarti Lea Yony Kunita Luqyana Ulfa Riyadi Nuniek Herminta Nurmayulis Reza Maulana Muhammad Rizky Puspita Sari Saiful Saiful Septi Yulianti Sri Rianawati Susiyanti . Susiyanti Susiyanti Widi Ayutami Yupi Isnaini Zahratul Millah