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AKTIVITAS ENZIM 2,4-D MONOOKSIGENASE DARI BERBAGAI MIKROBA [2,4-D monooxygenase Activity of Some Microorganisms] Sulistinah, Nunik
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Nine cultures both from fungi and bacteria have been selected for testing their 2,4-D monooxygenase activity to degrade 2,4-Dtohlorophenoxyacetic acid (2,4-D). The results showed that all the cultures which were tested grows at 1000 ppm 2,4-D. Three cultures (Trichoderma viride. Asperoillus niqer and Isolat E (unidentified)) of the nine cultures are able to grow at 4000 ppm. T. viride grows well on Minimal Basal Media which contained glucose and 2000 ppm 2,4-D and produced the highest biomass (0.8660 g/l) than the others. The biomass of T. viride grew on MBM (without glucose) and added with 2000 ppm 2,4-D is 0,6520g/l. This indicated that the culture is tolerant to 2,4-D and able to use 2,4-D compound as energy and carbon sources for its growth. But we failed to prove the 2,4-D monooxygenase activity of supernatant of T. viride by measuring the changing of pH-value in the 2,4-D breakdown reaction.

POTENSI Rhodococcus pyridinovorans GLB5 SEBAGAI BIOKATALIS DALAM KONVERSI SENYAWA METHIL SIANIDA DAN PHENIL SIANIDA [Potential of Rhodococcus pyridinovrans GLB5 as Biocatalistin Methyl and Phenyl Cyanide Conversion] Sulistinah, Nunik; Riffiani, Rini; Sunarko, Bambang
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Nitrile and amide bioconversion have received increasing attention due to their ability to provide a range of commercially important chemicals. The experiment was conducted to investigate the potential of bacterial isolate GLB5 to convert methyl cyanide and phenyl cyanide. The samples were collected from various industrial waste. Selection of isolates to utilize Â these substrates as a sole source of energy, carbon and nitrogen was conducted on 96 whell microtitter plates, based on the growth ability using INT (Iodo nitrotetrazolium chloride) reagent. Based on the growth Â pattern, it showed that the bacterial isolate GLB5 grew well and it was capable of utilizing Â methyl and phenyl cyanide compound as the sole source of carbon and nitrogen. Â The isolate GLB5 was isolated from industrial waste of Batik factory in Cirebon, and Â identified as Rhodococcus pyridinovorans. Bioconversion of methyl cyanide using whole cells of R. pyridinovorans GLB5 showed that ethanamide (C2H5NO) and ethanoic acid (C2H4O2) were detected. Formation of ethanamide and ethanoic acid as the product of bioconversion, indicated that the nitrile hydratase and amidase enzymes Â involved in the bioconversion process. Phenyl carboxamide (C7H7NO) as the product of phenyl cyanide bioconversion was also detected, Â although Â in Â low Â concentration. In this study, R. pyridinovorans GLB5 was capable of completely converting 300 mM methyl cyanide to Â± Â 140 mM ethanoic acid in relatively short times (<60 minutes).

KERAGAMAN BAKTERI PENGHASIL ENZIM PENGHIDROLISIS NITRIL DI PULAU ENGGANO BENGKULU [Diversity of Nitrilase Producing Bacteria in Enggano Island, Bengkulu] Riffiani, Rini; Sulistinah, Nunik
BERITA BIOLOGI Vol 16, No 3 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Potential nitrile degrading microbes have been isolated from marine sponge, marine water and soil in Enggano Island. Nitrilase enzyme has a function in degrading nitrile compund. Nitrilases are important industrial enzymes because of their ability to produce biologically active to degrade enantiomers, such as S-(+)-1-(4â-isobutylphenyl) propionic acid (S-(+)-ibuprofen) and R-(-) mandelic acid. Mandelic acids, which are important as pharmaceutical intermediates, can be produced in enantiomerically pure form by the hydrolysis of their corresponding nitrile. The aim of the study was to explore the diversity of nitrile degrading bacteria in Enggano Island, and their ability to utilize nitrile as a substrate growth. Screening of such microbes were carried out by using microtitter plate method based on growth ability tested by INT (Iodonitrotetrazoliumchloride). Degradation product was determined by High Performance Liquid Chromatography (HPLC). Seventy nine bacteria were able to grow on acetamide, acetonitrile, benzonitrile, adiponitrile, mandelonitrile, succinonitrile, lactonitrile, dan benzilcyanide as the sole source of carbon and nitrogen. Two isolates, YIM 56238 and PO69, have shown to enantioselectively hydrolyze racemic mandelonitrile to mandelic acid. Based on 16S rRNA gene identification, these bacteria have the highest sequence similarity to Microccous endophyticus strain YIM 56238 and Rhodococcus pyridinivorans strain PO69.

PERFORMA BAKTERIPADATANAH TERCEMAR PESTISIDA Rahmansyah, Maman; Sulistinah, Nunik
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Preliminary study on bacterial survive in soil containing pesticide has been carried out. Soil samples collected from Lembang and Dieng. The soil deprive from agriculture area that intensively using pesticide, and compared to other samples gathered from forest soil. All samples examined for total bacteria, denitrification bacteria, phosphate solubilizing bacteria, soil induce respiration, urease and phosphatase activities. Pattern of whole parameters in each soil sample configured similarly, but the value performed differently in the same parameters. Total bacterial population in soil samples also inspected after the samples amended with some certain pesticides. Survival bacteria subjected to media amended with insecticide (Propoxur, Diazinon, and Chlorpyrifos), and herbicides (Bromacil and 2,4-D), and correlation of bacterial growth between sample location were varied. Bacterial degrading pesticide particularly isolated from the soil samples containing 1000 ppm Curzate (fungiside) and 500 ppm 2,4-D.The isolates then cultured in the medium containing insecticide and herbicide, and the response on growth observed in 7 days incubation. Bacterial perform were meaningful to reference of soil degrading pesticide residue in agriculture soil; and it would make representative reference in an effort to use bacteria throughout biofertilizer improvement.

ISOLASI, SELEKSI, DAN KARAKTERISASI MIKROBA PENDEGRADASI ASETONITRIL DARI LIMBAH INDUSTRI Sunarko, Bambang; Adityarini, Adityarini; Tambunan, Usman Sumo F; Sulistinah, Nunik
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A number of microbes which could grow on acetonitrile were isolated and selected from industrial effluents and were studied to characterise the isolate which has the best degrading capability.Cultures were grown on mineral medium with microelements and acetonitrile was added as sole source of energy, carbon, and nitrogen.Isolate D5, identified as Corynebacteriumsp.,was able to grow on high concentration acetonitrile (up to 5 % v/v) and exhibited the highest specific growth rate (j).When Corynebacterium D5 grew on 2 % (v/v) acetonitrile,the doubling time was 6 hours 40 minutes,the specific growth rate (p) was 0.1 h and the acetonitrile decreasing rate was 3.99 mM/h.Increasing of acetonitrile concentration would extend the doubling time, decline the maximum growth and specific growth rate (i), and biomass production of Corynebacterium 05.The products of acetonitrile degradation by Corynebacterium D5 were acetamide, acetic acid, and ammonia.The maximum growth of Corynebacterium D5 showed when /3-aminopropionitrile was used as a substrate.

PERTUMBUHAN BEBERAPA ISOLAT MIKROBA DARI BERBAGAI LIMBAH INDUSTRI PADA BENZAMIDA Sulistinah, Nunik; Sunarko, Bambang
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Twenty five microbes could be isolated from industrial effluents.Seven isolates of those examined microbes were able to grow on benzamide as sources of carbon,energy,and nitrogen..The highest growth on benzamide was shown by bacterial isolate D1.Besides on benzamide, isolate D1 could grow on acetamide,acrylamide, benzamide,nicotinamide and propionamide, respectively.. On carboxylic acids, however isolate D1 could grow only on acetic acid, propionic acid, and benzoic acid as carbon and energy sources.When isolate D1 grew on 40 mM benzamide, the doubling time(j was 6 h 40 minutes, the specific growth rate (J) was 0,046 h the attained maximum cell biomass was 4.96 g cell dry weigtAiter medium, and the yield coefficient (Y) was 124 g cell dry weight/mole benzamide.

KINETIKA BIOTRANSFORMASI SUKSINONITRIL OLEH Pseudomonas sp. Sulistinah, Nunik; Sunarko, Bambang
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Succinic acid (HOOC(CH2)2COOH) is commercial compound that primarily used in food products. This compound also used in textile industry for colouring process, drugs industry, paint, pernist and photography slice. Succinic acid usually produced in industry by chemical reaction and produce cyanide acid as by product. The objective of this research was to study the utilization of Pseudomonas sp. in produce succinic acid and to characterize of involved enzymes. The results showed that the enzyme optimally produce in log phase. The production rate of succinic acid formation was 0,982 mM/ml.h with the decrease of substrate consumption was 1,235 mM/ml.h. The yield of succinic acid during 81 hours fermentation was 81%. The optimum pH, temperature, Km and Vran were 7, 27°C, 90 mM, and 0,0002 mM/ml.h.

ISOLASI, SELEKSI, DAN KARAKTERISASI MIKROBA PENDEGRADASI ASETONITRIL DARI LIMBAH INDUSTRI Bambang Sunarko; Adityarini Adityarini; Usman Sumo F Tambunan; Nunik Sulistinah
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i2.1151

A number of microbes which could grow on acetonitrile were isolated and selected from industrial effluents and were studied to characterise the isolate which has the best degrading capability.Cultures were grown on mineral medium with microelements and acetonitrile was added as sole source of energy, carbon, and nitrogen.Isolate D5, identified as Corynebacteriumsp.,was able to grow on high concentration acetonitrile (up to 5 % v/v) and exhibited the highest specific growth rate (\j).When Corynebacterium D5 grew on 2 % (v/v) acetonitrile,the doubling time was 6 hours 40 minutes,the specific growth rate (p) was 0.1 h and the acetonitrile decreasing rate was 3.99 mM/h.Increasing of acetonitrile concentration would extend the doubling time, decline the maximum growth and specific growth rate (\i), and biomass production of Corynebacterium 05.The products of acetonitrile degradation by Corynebacterium D5 were acetamide, acetic acid, and ammonia.The maximum growth of Corynebacterium D5 showed when /3-aminopropionitrile was used as a substrate.

AKTIVITAS ENZIM 2,4-D MONOOKSIGENASE DARI BERBAGAI MIKROBA Nunik Sulistinah
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1242

Nine cultures both from fungi and bacteria have been selected for testing their 2,4-D monooxygenase activity to degrade 2,4-Dtohlorophenoxyacetic acid (2,4-D). The results showed that all the cultures which were tested grows at 1000 ppm 2,4-D. Three cultures (Trichoderma viride. Asperoillus niqer and Isolat E (unidentified)) of the nine cultures are able to grow at 4000 ppm. T. viride grows well on Minimal Basal Media which contained glucose and 2000 ppm 2,4-D and produced the highest biomass (0.8660 g/l) than the others. The biomass of T. viride grew on MBM (without glucose) and added with 2000 ppm 2,4-D is 0,6520g/l. This indicated that the culture is tolerant to 2,4-D and able to use 2,4-D compound as energy and carbon sources for its growth. But we failed to prove the 2,4-D monooxygenase activity of supernatant of T. viride by measuring the changing of pH-value in the 2,4-D breakdown reaction.

PERTUMBUHAN BEBERAPA ISOLAT MIKROBA DARI BERBAGAI LIMBAH INDUSTRI PADA BENZAMIDA Nunik Sulistinah; Bambang Sunarko
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1104

Twenty five microbes could be isolated from industrial effluents.Seven isolates of those examined microbes were able to grow on benzamide as sources of carbon,energy,and nitrogen..The highest growth on benzamide was shown by bacterial isolate D1.Besides on benzamide, isolate D1 could grow on acetamide,acrylamide, benzamide,nicotinamide and propionamide, respectively.. On carboxylic acids, however isolate D1 could grow only on acetic acid, propionic acid, and benzoic acid as carbon and energy sources.When isolate D1 grew on 40 mM benzamide, the doubling time(\j was 6 h 40 minutes, the specific growth rate (\J) was 0,046 h'\ the attained maximum cell biomass was 4.96 g cell dry weigtAiter medium, and the yield coefficient (Y) was 124 g cell dry weight/mole benzamide.

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