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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology TEGAR: Journal of Teaching Physical Education in Elementary School Mitra Akademia: Jurnal Pengabdian Masyarakat Makara Journal of Science Environmental and Materials Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Endang Saepudin, Endang
Chemistry Department, Faculty of Mathematics and Science, University of Indonesia, Depok 16424, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Physics Social Sciences

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Penerapan Model Pembelajaran Kooperatif Tipe TGT dalam Pembelajaran Permainan Bola Besar Berorientasi Sepak Takraw untuk Meningkatkan Kerjasama dan Keterampilan Bermain Wahyudi, Widi; Budiman, Didin; Saepudin, Endang
TEGAR: Journal of Teaching Physical Education in Elementary School Vol 1, No 2 (2018): Teaching from the Perspective of Content Knowledge
Publisher : Prodi PGSD Penjas FPOK UPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/tegar.v1i2.11732

Abstract

Tujuan penelitian ini adalah untuk mengetahui dampak penerapan model kooperatif tipe TGT terhadap keterampilan bermain dan kerjasama dalam pembelajaran bola besar berorientasi sepak takraw. Metode penelitian yang digunakan adalah penelitian tindakan kelas (PTK) dengan memanfaatkan instrumen lembar observasi GPAI (game performance assestment instrument), catatan lapangan, dan dokumentasi penelitian. Partisipan penelitian ini adalah 30 siswa kelas V SDN Gegerkalong 1-2, Kota Bandung. Hasil analisis keterampilan bermain menunjukkan terjadinya kenaikan persentase dari observasi awal (15,55%) sampai tes siklus II tindakan II (75,83%). Tes kerjasama menunjukkan peningkatan persentase dari observasi awal (24,44%) sampai ke tes siklus II tindakan II (75,56%). Sehingga dapat disimpulkan bahwa aplikasi model pembelajaran kooperatif tipe TGT dalam pembelajaran bola besar berorientasi permainan sepak takraw dapat meningkatkan keterampilan bermain dan kerjasama  siswa kelas V SDN Gegerkalong 1-2 Kota Bandung. AbstrackThe aim of this study is to find out the impact of Cooperative Learning type TGT on students’ cooperation and skill in playing sepak takraw. The method used in this research was classroom action research (PTK) by using GPAI (game performance assestment instrument) observation sheets, field notes, and documentation as the instruments. The participants of the study were 30 fifthgrade students of SDN Gegerkalong 1-2, Bandung. The result of the skill analysis showed the improvement of the initial observation percentage (15,55%) to cycle II action II  percentage (75,83%). Meanwhile, the cooperation test showed that there was improvement on initial observation percentage (24,44%) to cycle II action II (75,56%). It concludes that the application of cooperative learning model type TGT in sepak takraw could improve students’ cooperation and skill in SDN Gegerkalong 1-2 Kota Bandung. 
Effect of Hydrolyzed Fucoidan from The Brown Seaweed Sargassum Binderi Sonder Towards Human Breast Cancer T47d Cell Lines Sinurat, Ellya; Saepudin, Endang; Qosthalani, Fildzah Alfita
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 2 (2017): August 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.127 KB) | DOI: 10.15578/squalen.v12i2.275

Abstract

Fucoidan, a sulfated heteropolysaccharide, consists of L-fucose and sulfate ester groups as the main component. Over the past three decades, fucoidan structures and bioactivities have been widely studied. The chemical components (fucose, galactose, small monosaccharides and also the sulfate) and the molecular weights of fucoidans from different brown seaweed species produce different characteristics and structures of fucoidan. The activity of fucoidan against cancer cells has been reported to be affected strongly by their sulfate content and molecular weight. Low-molecular-weight fucoidans tend to have higher solubility and easily penetrate into cancer cells. The objective of this study was to investigate the effect of hydrolyzed of fucoidan on its anti cancer activity againts the breast cancer T47D cells. In this study, the fucoidan from the brown seaweed Sargassum binderi Sonder was extracted using 0.1 N HCl and was depolymerized by acid hydrolysis at various times and concentrations. Result showed that fucoidan hydrolyzed with 1 M trifluoroacetic acid (TFA) for 1.5 hours reached the maximum depolymerization process and resulted in the decrement of molecular weight from 785.12 kDa to 5.79 kDa as well as sulfate content from 18.63% to 8.69%. The IC50 values of  fucoidan and low molecular weight fucoidan against the breast cancer T47D cells were 60.03 mg/mL and 182.34 mg/ respectively. This result indicated that the sulfate content of fucoidan probably affected its anticancer bioactivities. 
PELATIHAN PEMOGRAMAN PLC PADA SMKN 1 DI CILEUNGSI Darwin, Darwin; Syaprudin, Syaprudin; Saepudin, Endang
Mitra Akademia: Jurnal Pengabdian Masyarakat Vol 1, No 2 (2018): Mitra Akademia
Publisher : Unit Penelitian dan Pengabdian kepada Masyarakat (UP2M) Politeknik Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32722/mapnj.v1i2.1353

Abstract

Kegiatan Pengabdian Kepada Masyarakat sebagai salah satu Tri Dharma Perguruan Tinggi ini bertujuan tersedianya perangkat praktik pemograman PLC dan sarana penunjang lainnya di Sekolah Menengah Kejuruan Negeri 1 Cileungsi Bogor. Salah satu program keahlian yang terdapat di sekolah ini adalah Program Keahlian Otomasi. Tujuan Kompetensi Keahlian teknik Otomasi secara umum mengacu pada isi undangUndang Sistem Pendidikan Nasional (UU SPN) pasal 3 mengenai Tujuan Pendidikan Nasional dan penjelasan pasal 15 yang menyebutkan bahwa  pendidikan  kejuruan  merupakan  pendidikan  menengah  yang mempersiapkan peserta didik terutama untuk bekerja dalam bidang tertentu. Dengan tersedianya sarana praktik Pemograman PLC di SMK Negeri 1 Cileungsi, banyak manfaat yang dapat diperoleh terutama dalam meningkatkan kompetensi siswa Program Keahlian Teknik Otomasi. Diantara kompetensi dasar adalah Pemograman PLC. Dengan adanya pelatihan ini diharapkan siswa dapat mempraktikkan merancang bangun teknik Otomasi dengan menggunakan PLC sehingga dapat menjadi warga negara yang kompetitif, produktif, adaptif, selektif, dan kreatif. Human   Machine   Interface   (HMI)   merupakan   perangkat   antarmuka   antar pengguna dengan sistem kendali. Dengan perangkat ini suatu sistem dapat dimonitor, diatur, dan diambil datanya sesuai kebutuhan pengguna.  Pada Pengabdian Masyarakat ini dibuatlah sebuah modul latih PLC  dan HMI yang menggunakan   perangkat   HMI   Touchscreen Weintek MT8070IH5.  HMI Touchscreen berfungsi untuk memonitor, mengoperasikan dan mengontrol mesin atau proses di suatu plant dengan layar sentuh. Pada perangkat tersebut diatur sejalan dengan Proggramable Logic Controller (PLC) sehingga menghasilkan suatu sistem yang dapat dimonitor dan diatur secara baik. Untuk membuat perangkat ini bekerja secara bersama-sama perlu dilakukan pengaturan komunikasi pada alat dan dihubungkan oleh Ethernet.
Identification and Bioactivity Studies of Flavonoid Compounds from Macaranga hispida (Blume) Mull.Arg Megawati,; Saepudin, Endang; Hanafi, Muhammad; Darmawan, Akhmad; Lotulung, Puspa Dewi N.
Makara Journal of Science Vol. 19, No. 3
Publisher : UI Scholars Hub

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Abstract

Two flavonoid compounds, 5,7,3’,4’-tetrahydroxy-6-geranylflavonol (1) and kaempferol 7-O-β-glucose (2) have been isolated from the leaves of Macaranga hispida (Blume), Mull.Arg. Isolation and purification were conducted by chromatography methods and chemical structure characterization was carried out by spectroscopic methods. The 5,7,3’,4’-tetrahydrxyi-6-geranyl flavonol (1) and kaempferol 7-O-glucose (2) had moderate cytotoxic activity against murine leukemia P-388 cell lines with IC50 value of 0.22 and 101.5 μg/mL, respectively. The IC50 for antioxidant activities of (1) and (2) were 2.83 and 13.95 μg/mL, respectively. The LC50 of (1) and (2) from BSLT were 350 and >1000 μg/mL, respectively.
Enzymatic Hydrolysis of Mannan from Konjac (Amorphophallus sp.) Using Mannanase from Streptomyces lipmanii to Produce Manno-oligosaccharides Sasongko, Ashadi; Yopi,; Rahmani, Nanik; Lisdiyanti, Puspita; Saepudin, Endang
Makara Journal of Science Vol. 19, No. 3
Publisher : UI Scholars Hub

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Abstract

Mannan is an abundant polysaccharide that can be found in konjac (Amorphophallus sp.). Mannan can be enzymatically hydrolyzed using mannanase to produce manno-oligosaccharides which can be used as a prebiotic. The aims of this research are to determine the production time of mannanase from Streptomyces lipmanii, perform enzyme characterization, optimize the hydrolysis time, and characterize the hydrolysis product. A qualitative assay using the indicator Congo red showed that S. lipmanii generated a clear zone, indicating that S. lipmanii produced mannanase in konjac medium and possessed mannanolytic activity. Enzyme activity was determined through reducing sugar measurement using the dinitrosalycylic acid method, and optimum enzyme production was achieved at the second day of culture. Characterization of the enzyme showed that hydrolysis was optimum at pH 7 and at a temperature of 50 oC. The reducing sugar content was increased by an increasing the hydrolysis time, and reached an optimum time at 2 h. The degree of polymerization value of three was achieved after 2 h hydrolysis of mannan from konjac, indicating the formation of oligosaccharides. Analysis by thin layer chromatography using butanol, acetic acid, and water in a ratio of 2:1:1 as eluent showed the presence of compounds with a retention time between those of mannose and mannotetrose. Confirmation was also performed by HPLC, based on the retention time.
Electrochemical Detection of Neuraminidase Based on Zanamivir Inhibition Reaction at Platinum and Platinum-Modified Boron-Doped Diamond Electrodes Ivandini, Tribidasari Anggraningrum; Ariani, Jenny; Jiwanti, Prastika Krisma; Gunlazuardi, Jarnuzi; Saepudin, Endang; Einaga, Yasuaki
Makara Journal of Science Vol. 21, No. 1
Publisher : UI Scholars Hub

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Abstract

Neuraminidase sensors have been developed using platinum (Pt) and platinum-modified boron-doped diamond (Pt-BDD) electrodes based on the difference of electrochemical responses of zanamivir in the absence and in presence of neuraminidase. Cyclic voltammetry of zanamivir showed that the presence of zanamivir decreases the oxidation-reduction peak currents of Pt. The responses were found to be linear in the zanamivir concentration range of 7.5-150 μM. Comparison between Pt-BDD and Pt bulk electrodes showed that Pt-BDD offers approximately two times higher sensitivity of zanamivir responses than Pt bulk. Further comparison to gold-based electrodes showed that Pt-based electrodes provided about one order higher sensitivity of zanamivir responses. In the presence of neuraminidase, the oxidation-reduction currents of Pt again increase. Observation on the oxidation currents in the system with 2 x 10-5 M zanamivir showed that the responses were linear in the neuraminidase concentration range of 5-20 mU. Good reproducibility with an RSD < 3 (n = 10) was achieved even when the measurements were performed in a mucin matrix,suggesting that Pt-based electrodes are suitable to be applied for the detection method of neuraminidase.
Hemoglobin-Modified Core–Shell Fe3O4@Au Nanostructures for the Electrochemical Detection of Acrylamide Saepudin, Endang; Yuliani, Tri; Nasution, Mochammad Arfin Fardiansyah; Khalil, Munawar; Hong, Jong Wook; Ivandini, Tribidasari Anggraningrum
Makara Journal of Science Vol. 25, No. 3
Publisher : UI Scholars Hub

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Abstract

In this study, electrochemical detection of acrylamide using hemoglobin (Hb)-modified core–shell Fe3O4@Au nanostructures was conducted. Fe3O4 nanoparticles (~4.9 nm) and core–shell Fe3O4@Au (5.0–6.4 nm) nanostructures were successfully synthesized by the thermal decomposition method. Electrochemical investigation revealed that the optimum amount of Hb of 2 mg/mL could be immobilized in 0.1 M acetate buffer solution (pH = 6). Moreover, the detection of acrylamide using Fe3O4@Au/Hb was evaluated by the cyclic voltammetry technique. A linear calibration curve (R2 = 0.98) in the concentration range of 0.1 to 1.0 µM could be achieved with an estimated limit of detection, limit of quantification, and sensitivity of 0.136 µM, 0.453 µM, and 0.4411 µA/µM, respectively. Furthermore, the developed biosensor exhibited high selectivity in the presence of ascorbic acid, melamine, and caffeine. The developed biosensor was applied to the detection of acrylamide in coffee samples and validated using the standard high-performance liquid chromatography (HPLC) method. The concentration of acrylamide in coffee samples was determined to be 37.450 and 35.377 ppm using electrochemical measurement and HPLC, respectively.
Development of Immunochromatographic Strip Tests for Selective and Quantitative Detection of Melamine Wicaksono, Wiyogo P.; Ivandini, Tribidasari A.; Saepudin, Endang; Einaga, Yasuaki
Makara Journal of Science Vol. 18, No. 3
Publisher : UI Scholars Hub

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Abstract

An immunochromatographic strip test based on the complex reaction of antigen-antibody (melamine-antimelamine) was developed for quantitative detection of melamine. Gold nanoparticles (AuNP) were used to form AuNP-labeled antibody, which then acted as a biosensor. Melamine quantification was performed by the determination of AuNP using anodic stripping voltammetry technique with a boron-doped diamond as the working electrode. With sample volume of 100 µL and immunoreaction time of 7 min, the developed immunochromatographic strip test produced a linear calibration curve for melamine concentration range of 0–0.6 mg/L, with detection limit of 0.1 mg/L and RSD of ~5%. Furthermore, negative results were obtained for samples containing cyanuric acid and urea, indicating that the developed immunochromatographic strip test has potential for selective and quantitative detection of melamine.
THE DEVELOPMENT OF BIOCHEMICAL OXYGEN DEMAND SENSOR USING LOCAL YEAST: Candida fukuyamaensis, UICC Y-247 Anggraningrum, Ivandini Tribidasari; Zulfia, Fenny Triana; Saepudin, Endang
Makara Journal of Science Vol. 15, No. 1
Publisher : UI Scholars Hub

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Abstract

In order to shorten the measurement time of biochemical oxygen demand (BOD), a BOD sensor based on yeast metabolism was developed. Local yeast, Indonesian Origin, Candida fukuyamaensis UICC Y-247, was used as a transducer. The yeast was immobilized as a thin film in agarose matrix with the auxiliary of Nafion® acting as the membrane for ion exchange process. The film was then attached to gold-modified glassy carbons and used as transducer on the working electrodes. The measurements were conducted by observing the depletion of glucose concentration using multipulse amperometric method and then converted to BOD values. Optimum condition was observed in a waiting measurement time of 30 min at an applied potential of 450 mV (vs. Ag/AgCl). Linearity was shown in glucose concentration range of 0.1–0.5 mM, which was equivalent to BOD concentration range of 10–50 mg/L. A detection limit of 1.13 mg/L BOD could be achieved. Good repeatability was shown by a relative standard deviation (RSD) of 2.7% (n = 15). However, decreasing current response of ~50% was found after 3 days. Comparing to the conventional BOD measurement, this BOD sensor can be used as an alternative method for BOD measurements.
Carbon-coated nickel foam for hypochlorous acid sensor Sanjaya, Afiten Rahmin; Riyanto, Hanzhola Gusman; Rahmawati, Isnaini; Putri, Yulia Mariana Tesa Ayudia; Nurhalimah, Dede; Saepudin, Endang; Tesla, Yudistira; Krisnandi, Yuni Krisyuningsih
Environmental and Materials Vol. 1 No. 1: (June) 2023
Publisher : Institute for Advanced Science, Social, and Sustainable Future

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/eam.v1i1.2023.105

Abstract

The electrochemical detection method of hypochlorous acid (HOCl) using carbon foam electrodes have been successfully developed. The carbon foam was prepared from carbon-coated nickel foam which is synthesized using a hydrothermal-carbonization method. SEM characterization indicated that the optimum synthesis of carbon-coated nickel foam has been achieved on the 4th layer coating process with an expected spherical structure, while characterization using FTIR and Raman spectroscopy confirmed the formation of graphitic material with D band and G band characteristics. An electroactive surface area of ​​0.0236 cm2 was achieved. Cyclic voltammetry of HOCl in 0.1 M phosphate buffer solution pH 6.0 showed the best current response for HOCl reduction occurs at a potential of -0.3 V (vs. Ag/AgCl). Further detection of HOCl using amperometry technique in the concentration range of 2-200 μg/mL showed good linearity with sensitivity of 9.112 mA/µg/L and an estimated detection limit of 1.96 µg/mL. Good repetition is indicated by the RSD value of 2.499 % (n=5). This developed sensor also showed good selectivity in the presence of interference compounds, such as FeCl2 and CuSO4. Besides, the determination of HOCl in tap water has been successfully conducted and generated a comparable result with the UV-VIS method (3.30 µg/mL hypochlorite). The results indicated that the developed sensor is promising for the detection of HOCl in environmental applications.
Co-Authors Adiwinata, Syan Rosyid Afiten Rahmin Sanjaya Akhmad Darmawan Akhmad Darmawan Aliyah Ariani, Jenny Ashadi Sasongko Auditia, Ihsan Darwin Darwin Didin Budiman, Didin Einaga, Yasuaki Fatonah, Ani Fatonah Fenny Triana Zulfia Hong, Jong Wook Ivandini Tribidasari Anggraningrum Ivandini, Tribidasari A. Jarnuzi Gunlazuardi Jarnuzi Gunlazuardi Jenny Ariani Lestari K., Sri Lotulung, Puspa Dewi N. Maemonah, Maemonah Megawati Megawati Megawati, Muhammad Hanafi Muhammad Hanafi Munawar Khalil NANIK RAHMANI Nanik Rahmani Nasution, Mochammad Arfin Fardiansyah Nuralam Syamsuddin Nurhalimah, Dede Nurhidayat , Reza Rizqi Prastika Krisma Jiwanti Purwanti, B. S. R. Puspa Dewi N Lotulung PUSPITA LISDIYANTI Putri, Yulia Mariana Tesa Ayudia Qosthalani, Fildzah Alfita RAHMAT WIBOWO Rahmawati, Isnaini Riyanto, Hanzhola Gusman Sanjaya, Afiten Rahmin Sinurat, Ellya Suyanto Syaprudin, Syaprudin Tesla, Yudistira Tri Yuliani, Tri Tribidasari A Ivandini Wahyudi, Widi Wicaksono, Wiyogo P. Wiyogo P Wicaksono Yasuaki Einaga Yasuaki Einaga Yopi Yopi Yopi, Yuni Krisyuningsih Krisnandi, Yuni Krisyuningsih Zulfia, Fenny Triana