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Pengaruh Pemberian Pakan Buatan dengan Sumber Protein yang Berbeda Terhadap Efisiensi Pakan, Laju Pertumbuhan, dan Kelulushidupan Benih Abalone Hybrid Kuncoro, Aziz; Sudaryono, Agung; Suminto, -
Journal of Aquaculture Management and Technology Vol 2, No 3 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Feeding with artificial feeds in the abalone aquaculture is rarely conducted by abalone farmers. Feeding with artificial feed in the abalone aquaculture is rarely conducted by abalone farmers. Feeding with artficial feed in different protein sources is expected to produce a better quality abalone. This research aimed to determine the effects of different protein sources in artificial feeds on the on levels of feed intake, feed efficiency, growth rate and survival rate of hybrid abalone seeds and to know the best protein sources.This research used an experimental by using a completely randomized design (CRD) with 4 treatments, in 4 replicates, respeatively. The seeds of hybrid abalone used in this research were with average length size of 2,5 Â± 0,08 cm and average weight sized of 3,12 Â± 0,28 g. The treatments were (A) Awabi commercial feed from Japanese product; (B) feed with 100% from animals sources; (C) feed with 100% from vegetables sources; and (D) feed combination with 50% animals and 50% vegetables sources. This research was conrried in in November 2012 - January 2013 at Marine Culture Instute of Lombok. The results of this research indicated that feeding abalone with different protein sources was signifantly different (P<0,05) on levels of feed intake, highly significant effect (P<0,01) on the feed utilization efficiency and growth rate but no significant affect (Pâ‰¥0,05) was observed on the survival rate of hybrid abalone seeds. Awabi feed from Japanese product performed the best effect on the growth of hybrid abalone.

PENGARUH KONSENTRASI BAKTERI PROBIOTIK YANG BERASOSIASI DALAM USUS SEBAGAI BIOFLOK TERHADAP EFISIENSI PEMANFAATAN PAKAN, PERTUMBUHAN DAN KELULUSHIDUPAN LELE DUMBO (Clarias gariepinus) Simanjuntak, Iwan Chandra Binsar Hamonangan; Suminto, -; Sudaryono, Agung
Journal of Aquaculture Management and Technology Volume 5, Nomor 2, Tahun 2016
Publisher : Journal of Aquaculture Management and Technology

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Lele dumbo (Clarias gariepinus) merupakanÂ salahÂ satuÂ jenisÂ ikan airÂ tawar komersialÂ yang populer sebagai ikan budidaya. Dampak dari kegiatan budidaya intensif berpengaruh terhadap kesehatan lingkungan. Teknologi bioflok merupakan salah satu pemecahan masalah lingkungan dan dapat meningkatkan produksi budidaya. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan bakteri probiotik (Bacillus subtilis dan Bacillus licheniformis) yang berasosiasi dalam usus sebagai bioflok terhadap efisiensi pemanfaatan pakan, pertumbuhan dan kelulushidupan serta mengetahui konsentrasi terbaik untuk menghasilkan pertumbuhan dan kelulushidupan lele dumbo (C. gariepinus). Ikan uji yang digunakan adalah lele dumbo berukuran berukuranÂ 6-7 cm dan bobot 1,4Â±0,5 g. Metode penelitian menggunakan rancangan acak lengkap (RAL) dengan perlakuan A (tanpa probiotik), B (dosis probiotik 106 CFU/mL), C (dosis probiotik 107 CFU/mL), dan D (dosis probiotik 108 CFU/mL). Lele dumbo dipelihara dalam ember dengan volume 28 L selama 50 hari. Hasil pengamatan menunjukkan perlakuan D memiliki nilai EPP terbaik yaitu (67.20Â±3.33%), SGR (10.63Â±0.80%/hari) dan bobot mutlak (8.07Â±0,30 g). Penambahan probiotik pada media pemeliharaan menunjukkan pengaruh yang nyata terhadap efisiensi pemanfaatan pakan (EPP), SGR (Specific Growth Rate) dan laju pertumbuhan berat mutlak lele dumbo. Penelitian ini membuktikan bahwa penambahan dosis probiotik 108 CFU/mL yang terbaik meningkatkan pertumbuhan lele dumbo.Â Catfish (Clarias gariepinus) is one of freshwater fish as a popular commercial fish farming. The impact of intensive farming activities are influential on enviromental healthy. Biofloc technology is one of the environmental problem solvers and can be increase the production of aquaculture. The purpose of this research is to know the effect of bacterial probiotic addiction with different dosage associated in intestinal gut catfish on the feed utilization efficiency, growth and survival rate of catfish (C. gariepinus) and to know the best concentration to generate growth and survival rate of C. gariepinus. The fish samples used were catfishesÂ which have average length 6-7 cm and average wight 1,4Â±0,5 g. The experimental method was employed in this research with completely randomized design. The research was carried out 4 (four) treatments withÂ 3 (three) repetitions. Those treatments were A, treatment without addition probiotic; B, treatment with addition probiotic 106 CFU/mL; C, treatement with addition probiotic 107 CFU/mL and D, treatment with addition probiotic 108 CFU/mL that addedÂ the probiotic on the water. The fish cultivate for 50 days inÂ a bucket with volume 28 L. The results of this research prove that D treatment has the best EPP of (67.20Â±3.33%), SGR (10.63Â±0.80%/day) and the absolute weights (8.07Â±0,30 g). The use of probioticÂ had significally affected to efficiency of feed utilization, SGR (specific growth rate) and absolute weights ofÂ catfish, C. gariepinus. Therefore the probiotic treatment of 108 CFU/mL appear to be the best dosage for growth ofÂ C. gariepinus.

PERFORMA KEMATANGAN GONAD, FEKUNDITAS, DAN DERAJAT PENETASAN UDANG WINDU (Penaeus monodon Fab.) MELALUI SUBTITUSI CACING LAUT DENGAN CACING TANAH Pujianti, Puput; Suminto, -; Rachmawati, Diana
Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Pemberian subtitusi cacing laut dengan cacing tanah pada pembenihan udang windu diharapkan dapat meningkatkan kematangan gonad, fekunditas dan derajat penetasan. Penelitian ini bertujuan untuk mengetahui pengaruh subtitusi cacing laut dengan cacing tanah dan untuk mengetahui jumlah subtitusi cacing laut dengan cacing tanah yang terbaik terhadap kematangan gonad, fekunditas dan derajat penetasan pada induk udang windu (Penaeus monodon Fab.). Materi yang digunakan induk udang windu yang berasal dari Pangandaran dengan berat tubuh rata-rata 160.47Â±14.88 gram. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan yaitu perlakuan A (Cumi-cumi 30%, Tiram 40%, Cacing laut 30%), perlakuan B (Cumi-cumi30%, Tiram 40%, Cacing laut 22,5%, Cacing tanah 7,5%), perlakuan C (Cumi-cumi30%, Tiram 40%, Cacing laut 15%, Cacing tanah 15%), perlakuan D (Cumi-cumi30%, Tiram 40%, Cacing laut 7,5%, Cacing tanah 22,5%), dan perlakuan E (Cumi-cumi30%, Tiram 40%, Cacing tanah 30%) dengan 3 kali ulangan. Hasil penelitian menunjukkan bahwa pemberian subtitusi cacing laut dengan cacing tanah perlakuan A memberikan tingkat kematangan gonad paling cepat yaitu selama 4 â€“ 5 hari. Hasil itu menunjukkan bahwa pemberian subtitusi cacing laut dengan cacing tanah berpengaruh nyata (P<0,05) terhadap derajat penetasan (HR) dengan nilai tertinggi pada perlakuan B yaitu sebesar 94,40Â±1,49% tetapi tidak berpengaruh nyata (P>0,05) terhadap kematangan gonad dan fekunditas induk udang windu (P. monodon Fab.). Berdasarkan pada hasil penelitian dapat disimpulkan bahwa Pemberian cacing tanah sebanyak 25% dari total pemberian cacing laut dapat digunakan sebagai substitusi cacing laut dalam strategi pemberian pakan induk udang windu.Â The substitute mudworm with earthworm in the tiger prawn hatchery can be expected to increase the maturation, fecundity and hatching rate. The purpose of the research was to observe the effect of the substitute mudworm with earthworm and to find out the best quantity of substitute earthworm on the maturation, fecundity and hatching rate (HR) brood stock of tiger prawn. This experiment used black tiger shrimp brood stock from Pangandaran with 160.47Â±14.88 grams weight. This experiment used was experimental method with completely randomized design with 5 treatments, i.e. treatment A (30% squid, 40% oyster, 30%mudworm, 0% earthworm), treatment B (30% squid, 40% oyster, 22,5%mudworm, and 7,5% earthworm), treatment C (30% squid, 40% oyster, 15%mudworm, and 15% earthworm), treatment D (30% squid, 40% oyster, 7,5%mudworm and 22,5% earthworm) and treatment E (30% squid, 40% oyster, 0% mudworm, 30% earthworm). The result showed that the substitute mudworm with earthworm in the treatment A was the fastest on the maturation during 4 â€“ 5 days. the substitution treatments of mudworm with earthworm were significantly effect (P<0,05) on the Hatching Rate (HR) with the highest value in treatment B of 94,40Â±1,49% but not significantly effect (P>0,05) on the maturation and the fecundity of tiger prawn, P. monodon Fab. Base on the results and conclusion suggested that the gift ofÂ earthworm 25% of total giving mudwornÂ can usedÂ as substitution of Â mudworm Â in giving feed strategy for tiger prawn, P. monodon Fab.

PENGARUH KOMBINASI PAKAN ALAMI SEL FITOPLANKTON DAN BAHAN ORGANIK (BEKATUL, AMPAS TAHU, TEPUNG IKAN) YANG DIFERMENTASI TERHADAP PERFORMA PERTUMBUHAN Oithona sp. Afifah, Farida Nur; Suminto, -; Chilmawati, Diana
Journal of Aquaculture Management and Technology Volume 4, Nomor 4, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Oithona sp. merupakan salah satu jenis copepoda yang memungkinkan untuk dikembangkan sebagai substitusi pakan pengganti Artemia. Oithona sp. juga mempunyaiÂ kandungan nutrisi yang lebih baik dari Artemia. Penelitian ini telah dilakukan dengan tujuan untuk mengetahui pengaruh pemberian kombinasi pakan alami sel fitoplankton dan bahan organik (bekatul, ampas tahu, tepung ikan) yang difermentasi terhadap performa pertumbuhan Oithona sp., selain itu juga untuk mengetahui dosis kombinasi pakan alami sel fitoplankton dan bahan organik (bekatul, ampas tahu, tepung ikan) yang difermentasi yang memberikan performa pertumbuhan Oithona sp. terbaik.Metode yang digunakan selama penelitian Rancangan Acak Lengkap (RAL) dengan 5Â perlakuan 3 kali ulangan. Kultur Oithona sp. dilakukan di botol kaca dengan volume 10 mL dan kepadatan awal Oithona sp. 1 ind/mL. Pemeliharaan dilakukan selama 21 hari. Perlakuan dalam penelitian ini yaitu A (100% fitoplankton : 0% fermentasi), B (75% fitoplankton : 25% fermentasi), C (50% fitoplankton : 50% fermentasi), D (25% fitoplankton : 75% fermentasi), E (0% fitoplankton: 100% fermentasi). Pakan Alami sel fitoplankton yang digunakan yaitu Chaetoceros calcitrans dan Isochrysis galbana, sedangkan bahan organik yang digunakan yaitu bekatul ampas tahu dan tepung ikan dengan perbandingan 35%: 35%: 30%.Hasil penelitian menunjukkan pemberian kombinasi pakan alami sel fitoplankton dan bahan organik yang difermentasi berpengaruh nyata (P<0,05) terhadap performa pertumbuhan Oithona sp. Pemberian Â fitoplankton 50% dan fermentasi 50% merupakan dosis terbaik dalam penelitian ini. Kepadatan puncak Oithona sp. dengan pemberian fitoplankton 50% dan fermentasi 50% mencapai 14,333 Â± 0,115 ind/ml, sedangkan kepadatan naupli dengan pemberian fitoplankton 50% dan fermentasi 50% mencapai 6,930 Â± 0,360 ind/ml, kepadatan copepodit 7,470 Â± 0,321 ind/ml, kepadatan dewasa 8,970 Â± 0,500 ind/ml.Â Oithona sp. is one of copepods that possibly can be developed as substitution food replacement for Artemia. This study was done with the aims to determined the effect of combination of live food organisms of fitoplankton cells and fermented organic matters on the growth performances of Oithona sp., moreover to determine the dosage of fitoplankton cells and fermented organic matters that gives the good growthÂ performances of Oithona sp.The method experiment used in this research. There was designed by completely randomized design (CRD) with five treatments and three replicates respectively. Those treatments were A (100% phytoplankton: 0% fermentation), B (75% phytoplankton: 25% fermentation), C (50% phytoplankton: 50% fermentation), D (25% of phytoplankton: 75% fermentation), E (0% phytoplankton: 100% fermentation). The culture was conducted in glass bottle with 10 ml in volume and the initial density of 1 ind/ml. Maintenance was caried out for 21 days. Chaetoceros calcitrans and Isochrysis galbana used as live food in this research. Rice bran, tofu waste and fish meal used as organic matters with a ratio 35% : 35% : 30%. The results showed combination of live food organism of fitoplankton cells and fermented organic matters were significantly effected (P<0.05) on the growth performances of Oithona sp. The treatment of phytoplankton 50% and 50% fermented organic matters was the best dosage that gives the good growth performances of Oithona sp. The highest density reached 14.333 Â± 0.115 ind / ml, whereas naupli density, copepodit density, and adult density reached 6.930 Â± 0.360 ind / ml, 7.470 Â± 0.321 ind / ml, 8.970 Â± 0.500 ind / ml respectively.

PENGARUH BAKTERI PROBIOTIK PADA PAKAN DENGAN DOSIS BERBEDA TERHADAP EFISIENSI PEMANFAATAN PAKAN, PERTUMBUHAN DAN KELULUSHIDUPAN LELE DUMBO (CLARIAS GARIEPINUS) Wardika, Aziz Sinung; Suminto, -; Sudaryono, Agung
Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Probiotik merupakan mikroba hidup yang memiliki pengaruh menguntungkan bagi inang melalui modifikasi bentuk keterikatan (asosiasi) dengan inang atau komunitas mikroba lingkungan hidupnya sehingga dapat memiliki pengaruh memperbaiki kecernaan makanan, melindungi dari serangan patogen dan memperbaiki kualitas lingkungan. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan jumlah probiotik yang berbeda yang berasal dari usus lele terhadap efisiensi pemanfaatan pakan, pertumbuhan dan kelulushidupan lele dumbo (Clarias gariepinus). Hewan uji yang digunakan adalah lele dumbo sebanyak 240 ekor berukuranÂ 6-7 cm dan bobot 2,35 Â± 0,05 g. Bakteri probiotik yang digunakan sebagai bakteri uji adalah Bacillus subtilis, Bacillus lincheniformis dan Psidomonas putida telah diisolasi dari usus lele. Metode penelitian ini menggunakan rancangan acak lengkap (RAL) dengan perlakuan A (tanpa probiotik), B (dosis probiotik 106 CFU/mL), C (dosis probiotik 107 CFU/mL), dan D (dosis probiotik 108 CFU/mL) dengan menyemprotkan probiotik pada pakan. Hasil pengamatan menunjukkan perlakuan D memiliki nilai EPP terbaik yaitu (70,83Â±4,60 %), PER (2,23Â±0,15), SGR (2,61Â±0,06 %/hari), panjang mutlak (4,50Â±0,72 cm) dan mempunyai SR (96,67Â±0,24%). Penambahan probiotik pada pakan menunjukkan pengaruh yang nyata terhadap EPP (Efisiensi Pemanfaatan Pakan), PER (Protein Eficiency Ratio), SGR (Specific Growth Rate), Pertumbuhan panjang mutlak, dan tidak berpengaruh nyata terhadap serta SR (Survival Rate) lele dumbo. Hasil penelitian menunjukkan dosis probiotik terbaik yaitu perlakuan D (108 CFU/mL).Â Probiotic is a microbial life, that give to the advantageous effect for a fish culture (host) it through modification which have bounded (association) with host or microbial community of those habit so that have affected to improve feed utilization efficiency, resistance to pathogen and improve the environment quality. The purpose of this research was to know the effected of bacterial probiotic isolated from intestinal catfish (Clarias gariepinus) with the differentÂ dosage on the feed utilization efficiency, growth and survival rate of catfish (C. gariepinus). The fish samples used were 240 tails of total which have average length 6-7cm and average wight 2,35 Â± 0,05 g. The bacterial samples used were Bacillus subtilis, Bacillus lincheniformis and Psidomonas putida. The experimental method was employed in this research with completely randomized design. The research was carried out 4 (four) treatments withÂ 3 (three) replicates respectively. Those treatments were A treatment (no added probiotic), B treatment (with addition of 106 CFU/mL), C (with addition of 107 CFU/mL), and D (with addition of 108 CFU/mL) spraying the probiotic on the feed. The results showed that D treatment had the EPP of (70.83Â±4.60%), PER of (2.23Â±0.15), SGR of (2.61Â±0.06 %/day), absolute length (4.50Â±0.72 cm) and had SR (96.67Â±0.24 %). The use of probioticÂ had significally affected on EPP (feed efficiency utilization), PER (Protein Efficiency Ratio), SGR (Survival Growth Rate), absolute length for dumbo catfish, and Indicated that they were not significantly different on C. gariepinus SR (survival rate). Therefore the dosage of D treatment appears to be the best dosage.

STUDI POLA PERTUMBUHAN DAN KUALITAS SEL Chlorella sp. YANG DIHASILKAN MELALUI TEKNOLOGI PENCUCIAN BIBIT SEL Andreas, Sigmund Qory; Suminto, -; Chilmawati, Diana
Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Di dalam kultur massal Chlorella sp. sering terjadi penurunan jumlah sel secara drastis dan lama fase stasioner berselang kurang dari satu hari. Hal Â ini diduga karena terjadi hubungan tertutup antara bakteri kontaminan dengan Chlorella sp. di dalam kulturnya. Penelitian ini bertujuan untuk membersihkan Chlorella sp. dari bakteri kontaminan menggunakan teknologi pencucian bibit sel, sehingga dapat memperbaiki pola pertumbuhan dan kualitas sel yang dihasilkan. Metode penelitian ini adalah eksperimen, menggunakan RAL dengan 4 perlakuan dan 4 kali ulangan. Perlakuan itu adalah Chlorella sp. yang dikultur dengan tanpa pencucian bibit sel (A), dengan 1 kali pencucian (B), dengan 2 kali pencucian (C), dan dengan 3 kali pencucian (D). Variabel yang diamati yaitu pola pertumbuhan yang terdiri dari waktu adaptasi, laju pertumbuhan spesifik, lama waktu stasioner, kepadatan sel maksimum, kepadatan akhir kultur, dan kualitas sel dengan kandungan proteinya. Hasil penelitian menunjukkan bahwa pencucian bibit sel berpengaruh nyata (p<0,05) terhadap pola pertumbuhan sel Chlorella sp., terutama pada lama waktu fase stasioner dan nilai kepadatan maksimum sel. Lama waktu fase stasioner pada bibit sel yang mengalami pencucian 3 kali terjadi selama 5,5 hari (D), lebih lama dibandingkan dengan tanpa pencucian bibit sel yaitu selama 2 hari (A) dan kepadatan maksimum sel Chlorella sp. perlakuan D (5,2 X 107 sel/ml), lebih banyak dibandingkan perlakuan A (1,4 X 107sel/ml). Kandungan protein sel Chlorella sp. pada perlakuan A (52,52 %) lebih rendah dibandingkan pada perlakuan D (54,93%). Dari hasil tersebut di atas maka dapat disimpulkan bahwa pencucian bibit sel dapat memperbaiki pola pertumbuhan dan kandungan protein Chlorella sp. pada kulturnya.Â Mass culture of Chlorella sp. often occurred drastic decrease in the number of cells and a long in the stationary phase which less than one day. It is assumed that due to close correlation of bacterial contaminants on to Chlorella sp. cells in the culture. The aim of this study was cleaned seed cells of Chlorella sp. from the bacterial contaminants by washing cells technology so as to improve the growth pattern and quality of Chlorella cells. The experiment method was employed in this research. There was Completely Randomized Design method with 4 treatments and 4 replicaties, respectively. Those treatments were Chlorella sp. cells cultured with seed cells without washed (A), with one time washed (B), with two times washed (C) and with three times washed (D). Variables observed were growth pattern of Chlorella sp. cells ie: lag phase, specific growth rate, a long time of stationary phase, maximum cells density and the end of culture density, and cells quality with their protein content. The results showed that cells seed washing was significantly effect (p <0.05) on the growth pattern of Chlorella sp. cells, as specialy on the a long time of stationary phase and maximum density. The stationary phase for treatment which washed three times was 5.5 days, longer than the unwashed (2 days). Either on the cell maximum density that higher on treatment D (5.2 X 107 cell/ml) than treatment A (1.4 X 107cell/ml). The protein content also higher on treatment D (54.93%) than treatment A (52.52%). Those could be concluded that cells seed washing to maximalised the growth patterns and protein content of Chlorella sp. cells in culture.

PENGARUH PEMBERIAN RECOMBINANT GROWTH HORMONE (rGH) DENGAN DOSIS BERBEDA PADA PAKAN KOMERSIAL TERHADAP EFISIENSI PEMANFAATAN PAKAN, PERTUMBUHAN DAN KELULUSHIDUPAN BENIH IKAN PATIN (P. pangasius) Fissabela, Fika Ainallaudia; Suminto, -; Nugroho, Ristiawan Agung
Journal of Aquaculture Management and Technology Volume 5, Nomor 3, Tahun 2016
Publisher : Journal of Aquaculture Management and Technology

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Penelitian ini bertujuan untuk mengkaji pengaruh penambahan recombinant Growth Hormone (rGH) pada pakan komersial dengan dosis berbeda terhadap efisiensi pemanfaatan pakan, pertumbuhan dan kelulushidupan dan mengetahui perlakuan pemberian recombinant Growth Hormone (rGH) pada pakan komersial yang memberikan efisiensi pemanfaatan pakan, pertumbuhan dan kelulushidupan ikan patin (P. pangasius) yang terbaik. Penelitian ini dilaksanakan di Laboratorium Budidaya Perairan Universitas Diponegoro, Semarang, Jawa Tengah Tengah pada 14 November â€“ 15 Januari 2016. Ikan uji yang digunakan dalam penelitian ini adalah ikan patin (P. Pangasius), dengan benih 0,9 - 1,4 g. Penelitian ini dilakukan dengan metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian ini yakni, perlakuan A (pakan + 0 mg/kg), B (pakan + 1 mg/kg), C (pakan + 2 mg/kg), dan D (pakan + 3 mg/kg). Pembuatan larutan rGH untuk 1 kg pakan adalah pencampuran rGH sesuai dosis ditambah kuning telur 20 ml + larutan Phosphate Buffered Saline (PBS) 100 ml, dicampur & disemprotkan secara merata ke pakan dan diberikan secara at satiation sehari 2 kali. Data yang diamati meliputi TKP, EPP, PER, SGR, SR dan kualitas air. Data dianalisa menggunakan ANOVA untuk melihat perbedaannya, kemudian jika terdapat perbedaan maka dilakukan uji wilayah Duncan untuk melihat perlakuan terbaik. Pendederan benih berlangsung selama 63 hari. Kesimpulan dari hasil penelitian menunjukkan bahwa kombinasi rGH dan pakan komersial, menunjukkan pengaruh nyata (P<0,05) terhadap TKP, EPP, PER, SGR, panjang mutlak dan SR. Perlakun terbaik terhadap TKP, EPP, PER, SGR, dan SR adalah perlakuan C dengan nilai TKP sebesar 670,36Â±26,11 gram, EPP sebesar 58,49Â±0,92%, PER sebesar 1,13Â±0,02%, SGR sebesar 4,60Â±0,02%, dan kelulushidupan sebesar 73,33Â±2,89%. Sehingga perlakuan penambahan rGH dengan dosis 2 mg/kg menghasilkan efisiensi pemanfaatan pakan, pertumbuhan dan kelulushidupan terbaik untuk ikan patin (P. pangasius).Â This research was aimed to assess the effect of giving recombinant Growth Hormone (rGH) in commercial feed with different dosage for food utilization efficiency (FCE), growth and survival rate (SR); and determine the exact dosage of recombinant Growth Hormone (rGH) that provides the best result on food conversion efficiency, growth and survival rate of pangasius fish (P. pangasius). This research was conducted at the Aquaculture Laboratory, University of Diponegoro, Semarang, Central Java on November 14 to January 15, 2016. The trial fish used in this research was Fry Pangasius (P. pangasius) with average weight of seed about 0,9 to 1,4 g. The experimental design used was completely randomized design with 4 treatments and 3 replicates. Which were treatment A (feed + 0 mg / kg); B (feed + 1 mg/kg); C (feed + 2 mg/kg); and D (feed + 3 mg/kg). of rGH for 1 kg of feed is the appropriate dose of rGH mixed with 20 ml egg yolk + solution Phosphate Buffered Saline (PBS) 100 ml, mixed and sprayed evenly to feed. The methode been used was at satiation (2 times/day). The data observed were involving Food Consumption Rate, Food Utilization Efficiency, Protein Efficiency Ratio (PER), Spesific Growth Rate (SGR), absolute length, SR and water quality. Data were analyzed by Â using ANOVA to see the difference, then if there is a difference then tested with Â Duncan area to see the best treatment. Trial fish was reared for 63 days. The conclusions shown that the combination of rGH and commercial feed were giving affect significantly (p<0,05) for Food Consumption Rate, Food Utilization Efficiency, PER, SGR, absolute length and SR. Best treatment that affected the value of Food Consumption Rate, Food Utilization Efficiency, PER, SGR, absolute length and SR is treatment C with value of Food Consumption Rate 670,36Â±26,11 grams, Food Utilization Efficiency 58,49Â±0,92%, PER 1,13Â±0,02%, SGR 4,60Â±0,02%, the absolute length 9,78Â±0,015 cm and SR 73,33Â±2,89%. The addition of rGH with dosage of 2 mg/kg was the best treatment for food utilization efficiency, growth and survival rate of Pangasius (P. pangasius).

PRODUKSI NAUPLII DAN COPEPODIT Oithona sp. YANG DIKULTUR DENGAN PERBEDAAN DIET MIKROALGA (Chlorella vulgaris, Chaetoceros calcitrans, DAN Isochrysis galbana) Syarifah, Dian Hidayah; Suminto, -; Chilmawati, Diana
Journal of Aquaculture Management and Technology Volume 4, Nomor 3, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Beberapa penelitian menunjukkan bahwa Oithona sp. dapat digunakan sebagai pakan alami kegiatan budidaya air laut. Nauplii dan copepodit Oithona sp. mempunyai ukuran yang sesuai untuk pakan pertama larva ikan.Perlu dilakukan kajian tentang produksi Oithona sp. agar mencapai maksimal sehingga mampu mencukupi kebutuhan dalam kegiatan budidaya. Kajian 5 diet mikroalga dilakukan untuk mengetahui pengaruhnya terhadap produksi nauplii dan copepodit sekaligus untuk mendapatkan diet dengan hasil terbaik pada kedua stadia tersebut. Penelitian eksperimental laboratoris ini dilakukan di Laboratorium Pakan Hidup Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara dengan rancangan percobaan acak lengkap (RAL) masing-masing 3 pengulangan untuk setiap perlakuan. Perlakuan diet berdasarkan pada dosis 0.01 mg berat kering mikroalga untuk setiap satu individu copepoda. Kelima perlakuan diet untuk kultur Oithona sp. selama 22 hari adalah C. vulgaris (Cv); Cv+I. galbana (Ig) (1:1); Cv+C. calcitrans (Cc) (1:1); Cc+Ig (1:1); dan Cv+CC+Ig (1:1:1). Kultur Oithona sp. dilakukan pada botol kaca vial 50 ml dengan volume air laut 10 ml dan kepadatan awal Oithona sp. stadia dewasa 1 ind.ml-1. Hasil penelitian menunjukkan bahwa pemberian diet mikroalga yang berbeda berpengaruh nyata (P<0,05) pada produksi nauplii dan copepodit Oithona sp. Kepadatan nauplii (39,83 Â± 2,334 ind.ml-1) dan copepodit (12,93 Â± 0,170 ind.ml-1) adalah maksimum pada hari ke 22. Berdasarkan hasil penelitian ini disimpulkan bahwa diet Cc+Ig dan Cv+Cc+Ig masing-masing menghasilkan produksi nauplii dan copepodit terbaik. Diet Cc+Ig disarankan sebagai diet untuk pengembangan kultur Oithona sp. selanjutnya.Â Many research had showed that Oithona sp. could be used as a live food on marine culture activity. Nauplii and copepodit Oithona sp. have the suitable size for the fish fry. A studied about Oithona sp. production must be done to get the maximum production so it can fulfill the need of culture. The studied of 5 microalgal diet purposed to look for the diet effect for nauplii and copepodit production and also to got the best production of both stadia. This experimental laboratoris had done in Live Feed Laboratorium of Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara with Completely Randomized Design (CRD) triplicate for each treatment. Microalgal diet treatment based on 0.01 mg microalgal dry weight for one individu of copepod. That five trathments for 22 days Oithona sp. culture were C. vulgaris (Cv); Cv+ I. galbana (Ig) (1: 1); Cv+ C.calcitrans (Cc) (1:1); Cc+Ig (1:1); and Cv+Cc+Ig (1:1:1). Oithona sp. cultured on 50 ml vial glass bottle with 10 ml seawater and initial density of Oithona sp. adult stage was 1 ind.ml-1. The experimental result showed that the given of different microalgal diet had Â significant effect Â (P< 0,05) for nauplii and copepodit production of Oithona sp. Density of nauplii (39,83 Â± 2,334 ind.ml-1) and copepodit (12,93 Â± 0,170 ind.ml-1) were maximum on 22 day of culture respectively. Conclusion based on this experimental result was Cc+Ig and Cv+Cc+Ig diet produced the best result each on nauplii and copepodit density. Cc+Ig diet was suggested as the diet for the next development of Oithona sp. culture.

APLIKASI FEEDING REGIMES YANG BERBEDA TERHADAP TINGKAT KONSUMSI PAKAN ALAMI, PERKEMBANGAN DAN KELULUSHIDUPAN LARVA UDANG WINDU (Penaeus monodon) Nofiyanti, Vika Ratna; Subandiyono, -; Suminto, -
Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Penerapan feeding regime yang diterapkan pada panti pembenihan larva udang windu (P. monodon) di Indonesia pada umumnya menggunakan pakan Skeletonema sp., Artemia sp. dan pakan buatan.Â Dugaan masalah muncul karena jenis, ukuran, nutrisi dan dosis pakan yang diberikan kurang sesuai dengan kebutuhan pakan larva udang.Â Komposisi pakan dalam feeding regime yang tepat dan sesuai kebutuhan berpengaruh terhadap perkembangan larva udang.Â Tujuan dari penelitian ini adalah mengkaji aplikasi feeding regimes yang berbeda terhadap perkembangan dan tingkat kelulushidupan pada larva udang.Â Metode dalam penelitian ini adalah metode eksperimen yang menggunakan pola rancangan acak lengkap (RAL) dengan 3 perlakuan dan 3 ulangan.Â Perlakuan yang diberikan ialah feeding regime A (Chaetoceros sp., Skeletonema sp., Branchionus sp., Instar I Artemia sp. dan pakan buatan), feeding regime B (Chaetoceros sp., Skeletonema sp., Instar I Artemia sp. dan pakan buatan) dan feeding regime C (Skeletonema sp., Instar I Artemia sp. dan pakan buatan).Â Â Â Hasil penelitian menunjukkan bahwa feeding regimes yang berbeda memberikan pengaruh yang nyata (P<0,05) terhadap perkembangan dan tingkat kelulushidupan pada larva udang windu.Â Nilai perkembangan selama 9 hari penelitian mencapai stadia PL-1 yang tercepat ditunjukkan oleh feeding regime A sebesar 100Â±0%, sedangkan untuk feeding regime B dan C masing-masing sebesar 33Â±0% dan 0Â±0%, secara berurutan.Â Nilai tingkat kelulushidupan stadia PL-10 yang terbaik ditunjukkan oleh feeding regime A sebesar 20,00Â±1,32%, sedangkan pada feeding regime B dan C masing-masing sebesar 15,33Â±0,76% dan 11,17Â±1,15%, secara berurutan.Â Disimpulkan bahwa feeding regime A (Chaetoceros sp., Skeletonema sp., Branchionus sp., Instar I Artemia sp. dan pakan buatan) menunjukkan hasil yang terbaik dan direkomendasikan untuk diterapkan dalam feeding regime pada pembenihan larva udang windu (N hingga PL-10).Â Application of feeding regime which were applied on the hatchery of P. monodon larvae in Indonesia used Skeletonema sp., Artemia sp., and artificial feed.Â Problems may arise due to the type, size, and improper dose of the nutrients to meet the requirement of the shrimp larvae.Â Suitable composition in feeding regime to meet its requirement affected on the larval morphological development.Â The objectives of the research were to examine the application of various feeding regimes for morphological development and survival rate of the shrimp larvae.Â The experiment method was applied in this research with completely randomized design (CRD).Â The experiment was used three treatments and three replicates.Â Those treatments were feeding regime A (Chaetoceros sp., Skeletonema sp., Branchionus sp., Instar I of Artemia sp., and artificial feed), feeding regime B (Chaetoceros sp., Skeletonema sp., Instar I of Artemia sp., and artificial feed), and feeding regime C (Skeletonema sp., Instar I of Artemia sp., and artificial feed). The results showed that the application of different feeding regimes resulted on significantly different effect (P<0.05) on morphological development and survival rate of the trial larvae.Â The best value was also resulted on feeding regime A, with its total value of morphological development (100Â±0%) occurred at the day-9 was already at the PL-1 stage, whereas for feeding regime B and C were 33Â±0% and 0Â±0%, respectively.Â The best value was also resulted on feeding regime A, with its total value of survival rate (20.00Â±1.32%) occurred at theÂ Â Â day-18 was already at PL-10 stage, whereas for feeding regime B and C were 15.33Â±0.76% and 11.17Â±1.15%, respectively.Â Conclusion that feeding regime A i.e. Chaetoceros sp., Skeletonema sp., Branchionus sp., Instar I of Artemia sp., and artificial feed, its the best resulted and suitable to be applied to the feeding regime of the shrimp larvae hatchery (N up to PL-10).

PERFORMA KEMATANGAN GONAD, FEKUNDITAS DAN DERAJAT PENETASAN MELALUI PEMBERIAN KOMBINASI PAKAN ALAMI PADA INDUK UDANG WINDU (Penaeus monodon Fab.) Sabrina, -; Suminto, -; Rachmawati, Diana
Journal of Aquaculture Management and Technology Volume 3, Nomor 3, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Pemberian kombinasi pakan alami cumi-cumi (Loligo sp.), cacing laut (Marphysa sp.) dan tiram (Crassostrea sp.) pada pembenihan udang windu diharapkan dapat meningkatkan kematangan gonad, fekunditas dan derajat penetasan. Penelitian ini bertujuan untuk mengetahui pengaruh berbagai kombinasi pakan alami cumi-cumi, cacing laut dan tiram terhadap kematangan gonad, fekunditas dan derajat penetasan pada induk udang windu (P. monodon Fab.). Variabel yang diamati meliputi kematangan gonad, fekunditas dan derajat penetasan (HR). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan kombinasi pakan alami dan 3 kali ulangan. Perlakuan itu adalah A (cumi-cumi 25%, cacing laut 50% dan tiram 25%), B (cumi-cumi 30%, cacing laut 40% dan tiram 30%), C (cumi-cumi 35%, cacing laut 30% dan tiram 35%) dan D (cumi-cumi 40%, cacing laut 20% dan tiram 40%). Hasil penelitian menunjukkan bahwa pemberian kombinasi pakan alami pada perlakuan C memberikan tingkat kematangan gonad paling cepat yaitu selama 5 â€“ 6 hari. Penelitian ini juga menunjukkan bahwa derajat penetasan (HR) pada perlakuan A, B dan C berbeda nyata (P<0,05) terhadap perlakuan D tetapi tidak berbeda nyata (P>0,05) terhadap fekunditas induk udang windu (P. monodon Fab.). Berdasarkan pada hasil penelitian dapat disimpulkan bahwa pemberian kombinasi pakan alami dengan jumlah cacing laut 30 â€“ 50% dapat meningkatkan persentase derajat penetasan (HR) pada induk udang windu (P. monodon Fab.).Â The combination of live food organisms of squid (Loligo sp.), mudworm (Marphysa sp.) and oyster (Crassostrea sp.) in the tiger prawn hatchery tiger prawn, itâ€™s can be supposed to be increase the maturation, fecundity and hatching rate. The purpose of this research was to observe the effect of the various combination of live food organisms of squid, mudworm and oyster on the maturation, fecundity and hatching rate (HR) broodstock of tiger prawn. The variables measurement were maturation, fecundity and hatching rate (HR). This research was used completely randomized design (CRD) with 4Â treatments and 3 replicates, respectively.Â Those treatments were A (25% squid, 50% mudworm and 25% oyster), B (30% squid, 40% mudworm and 30% oyster), C (35% squid, 30% mudworm and 35% oyster) and D (40% squid, 20% mudworm and 40% oyster). The result showed that the combination of live food organisms in treatment C was the fastest on the maturation during 5 â€“ 6 days. This research was also showed that the Hatching Rate (HR) in treatment A, B and C were significanly effect (P<0,05) on the treatment D but no significanly effect (P>0,05) on the broodstock fecundity of tiger prawn, P. monodon Fab. Base on the results suggested that the combination of live food organisms in the ranged of mudworm 30 â€“ 50% could to increase the prosentage HR of tiger prawn, P. monodon Fab.

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