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All Journal KURVA S JURNAL MAHASISWA Jurnal Ilmu Kelautan Spermonde Aquacultura Indonesiana Journal of Fisheries & Marine
Andi Parenrengi
Balai Penelitian dan Pengembangan Budidaya Air Payau
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education

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The Morphometric Character and Mitochondrial 16S rRNA Sequence of Portunus pelagicus Fujaya, Yushinta; Hidayani, Andi Aliah; Asphama, Andi Ivo; Trijuno, Dody Dharmawan; Tenriulo, Andi; Parenrengi, Andi
Aquacultura Indonesiana Vol 16, No 1 (2015): Volume 16 Issue 1 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.576 KB) | DOI: 10.21534/ai.v16i1.1

Abstract

Blue swimming crab (Portunus pelagicus) colors have variations from different habitats and they have different vitality. The purpose of this study was to analyze the diversity of the P. pelagicus using morphometric and 16SrRNA mitochondrial sequence.  There were three groups of blue swimming crab characterized by differences in color and density patterns of spots.  The result of Canonical discriminant analysis showed that there were morphometric differences among the three groups.  The main distinguishing morphometric characteristics are the ratio of carapace width and length as well as the ratio of the length and width of cheliped merus.  The third group of crab is also has similarity about 98-99% by the Portunus pelagicus 16S rRNA sequences available in gene banks.  These studies had concluded that differences in color and pattern of the spot density have also morphometric and genetic differences. At least there were two groups based on genetic variation of Portunus pelagicus from Barru Regency waters which may have a variety of different properties.  Further research is needed to study the adaptation abilities of the two groups of crab on the condition of aquaculture as a potential domestic animal.Keywords: Crab; Portunus pelagicus; Morphometric; 16S rRNA.
ANALISIS BIAYA OPERASIONAL KENDARAAN RINGAN PADA PEMILIHAN RUTE KOTA SANGATTA – SIMPANG PERDAU KM. 106 DESA TEPIAN INDAH PARENRENGI, ANDI
KURVA S JURNAL MAHASISWA Vol 11, No 2 (2020)
Publisher : KURVA S JURNAL MAHASISWA

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Abstract

Dalam melakukan perjalanaan dari daerah satu ke daerah lainnya biasanya dapat ditemukan beberapa rute yang bisa di lewati untuk sampai ke tujuan tersebut. Banyaknya pilihan jenis rute lalu lintas yang bisa ditempuh untuk menuju suatu daerah menuntut penguna jalan untuk memilh rute paling efektif, dengan maksud untuk mengefisiensikan jarak, waktu, dan biaya operasional yang dibutuhkan untuk mencapai tujuan tersebut. Simpang Perdau KM. 106 Desa Tepian Indah merupakan simpang yang menghubungkan beberapa kecamatan di Kabupaten Kutai Timur dan juga akses yang dilewati untuk melakukan perjalanan dari Kabupaten Kutai Timur menuju Kabupaten Berau ataupun sebaliknya, dari Kota Sangatta menuju Simpang Perdau KM. 106 Desa Tepian Indah kebanyakan masyarakat lebih memilih untuk melewati rute Jalan Poros Rantau Pulung dibandingkan melewati rute jalan Poros Perdau dikarenakan rute tersebut dianggap terpendek, padahal belum tentu rute tersebut optimal dari segi waktu tempuh maupun biaya operasional kendaraan. Metode yang digunakan untuk mengukur kecepatan pada penelitian adalah metode kecepatan bergerak (running speed) yaitu kecepatan rata-rata pada suatu jalur pada saat kendaraan bergerak, di dapat dari membagi panjang jalur dengan lama waktu kendaraan bergerak menempuh jarak tersebut tanpa menghitung waktu henti. Sedangkan untuk analisis biaya operasional menggunakan metode PCI (Pasific Consultant International). Dari hasil perhitungan dapat disimpulkan bahwa perjalanan dari Kota Sangatta menuju Simpang Perdau KM. 106 Desa Tepian Indah, rute yang paling efektif dari segi biaya operasional kendaraan adalah rute Jalan Poros Perdau dibandingkan dengan rute Jalan Poros Rantau Pulung walaupun rute tersebut memiliki selisih jarak tempuh kurang lebih 19,3 KM dimana rute Jalan Poros Rantau Pulung lebih pendek daripada rute Jalan Poros Perdau.
EVALUATING THE DIVERSITY AND ABUNDANCE OF MARINE FISH IN THE MAKASSAR STRAIT, INDONESIA THROUGH ENVIRONMENTAL DNA (EDNA) ANALYSIS Rukminasari, Nita; Hidayani, Andi Aliah; Moka, Wilma J.C.; Arbit, Nur Indah Sari; Andriyono, Sapto; Parenrengi, Andi
Jurnal Ilmu Kelautan SPERMONDE VOLUME 10 NOMOR 2, 2024
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jiks.v10i2.32405

Abstract

The recent adoption of environmental DNA (eDNA) represents an innovative method for assessing the presence of aquatic vertebrate species, providing a relatively straightforward approach with significant implications for conservation biology. In our investigation, we employed eDNA metabarcoding to explore the diversity of fish in the Makassar Strait. We collected eDNA from samples taken both at the surface and from the water column (15 m depth) at two specific locations within this region. The reliability of the MiFish-U primer set methodology in estimating fish diversity in the Makassar Strait was assessed. In a single survey, based on four water samples from the Makassar Strait, we successfully identified 11 marine fish taxa at the species level. These taxa belong to 8 families across 8 orders. The predominance of reef-dwelling species suggests that coral reefs play a dominant role as the primary ecosystem in this area. Among the surveyed sites, Barru Waters exhibited the highest species richness (7 species), while Pangkep Waters only revealed 4 species. Through the application of eDNA metabarcoding, this study provided a means to assess fish diversity, delivering crucial foundational information. Our findings highlight the cost-effectiveness of the eDNA metabarcoding method as a powerful scientific tool for the management and conservation of marine fish resources in the Makassar Strait.    
Assessing various administration strategies for dsRNA vaccine delivery: a concise review of VP15-WSSV research progress in tiger shrimp Penaeus monodon Parenrengi, Andi; Lante, Samuel; Suryati, Emma; Rosmiati, Rosmiati; Sulaeman, Sulaeman; Herlinah, Herlinah; Andriani, Yosie
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 3 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i3.73580

Abstract

Graphical Abstract     Highlight Research 1. The dsRNA application should correspond to the developmental stages of shrimp2. Immersion method was commonly used for larvae for handling many individuals3. Injection technique effectively delivered dsRNA to cells but was unsuitable for large-scale4. Oral administration of pellet-enriched dsRNA was applicable to apply on shrimp larvae, juveniles, and broodstocks     Abstract RNAi technology offers a novel powerful approach to silence gene expression by introducing double-stranded RNA (dsRNA) into the cell to degrade the mRNA at the post-transcriptional stage. An administration method of dsRNA delivery is one of the main considerations in applying the dsRNA vaccine for controlling pathogen infections. This mini-review was focused on the evaluation of three different methods (immersion, injection, and oral administration) of VP15-dsRNA vaccine delivery to the tiger shrimp post-WSSV challenge test. The immersion method was generally applied for the larval stage of tiger shrimp and seemed to be a simple technique for a large number of individuals in a small tank. The VP15-dsRNA application by immersion improved the survival of tiger shrimp larvae by 3.9% compared to the control groups. The injection technique was an effective way to deliver dsRNA to the cell, but it is difficult to apply in a large number of individuals or populations. The injection of VP15-dsRNA increased significantly the survival rate, proPO, and THC of tiger shrimp. A higher survival rate (75%) was exhibited in tiger shrimp injected with in vivo and in vitro VP15-dsRNA than in the control. Oral administration by pellet-enriched VP15-dsRNA was a useful way for larvae, juveniles, and broodstocks, but it has limitations since the pellet leaches into the water. The application of the VP15-dsRNA vaccine on the feed significantly enhanced the 26.7% higher survival rate compared to the control. The higher survival was also supported by a higher number of THC. The three VP15-dsRNA delivery methods provide potential approaches to increase tiger shrimp resistance to control pathogen infection
Co-Authors Andi Aliah Hidayani Andi Tenriulo Asphama, Andi Ivo Dody Dharmawan Trijuno, Dody Dharmawan Emma Suryati Herlinah Herlinah Moka, Wilma J.C. Nita Rukminasari Nur Indah Sari Arbit Rosmiati Rosmiati Samuel Lante, Samuel Sapto Andriyono Sulaeman Sulaeman Yosie Andriani Yushinta Fujaya