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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology KURVA S JURNAL MAHASISWA Jurnal Ilmu Kelautan Spermonde Indonesian Aquaculture Journal Aquacultura Indonesiana Jurnal Ilmiah Ilmu Komputer Fakultas Ilmu Komputer Universitas Al Asyariah Mandar Journal of Fisheries & Marine
Andi Parenrengi
Balai Penelitian dan Pengembangan Budidaya Air Payau
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Immunology & microbiology Veterinary

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The Morphometric Character and Mitochondrial 16S rRNA Sequence of Portunus pelagicus Fujaya, Yushinta; Hidayani, Andi Aliah; Asphama, Andi Ivo; Trijuno, Dody Dharmawan; Tenriulo, Andi; Parenrengi, Andi
Aquacultura Indonesiana Vol 16, No 1 (2015): Volume 16 Issue 1 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.576 KB) | DOI: 10.21534/ai.v16i1.1

Abstract

Blue swimming crab (Portunus pelagicus) colors have variations from different habitats and they have different vitality. The purpose of this study was to analyze the diversity of the P. pelagicus using morphometric and 16SrRNA mitochondrial sequence.  There were three groups of blue swimming crab characterized by differences in color and density patterns of spots.  The result of Canonical discriminant analysis showed that there were morphometric differences among the three groups.  The main distinguishing morphometric characteristics are the ratio of carapace width and length as well as the ratio of the length and width of cheliped merus.  The third group of crab is also has similarity about 98-99% by the Portunus pelagicus 16S rRNA sequences available in gene banks.  These studies had concluded that differences in color and pattern of the spot density have also morphometric and genetic differences. At least there were two groups based on genetic variation of Portunus pelagicus from Barru Regency waters which may have a variety of different properties.  Further research is needed to study the adaptation abilities of the two groups of crab on the condition of aquaculture as a potential domestic animal.Keywords: Crab; Portunus pelagicus; Morphometric; 16S rRNA.
ANALISIS BIAYA OPERASIONAL KENDARAAN RINGAN PADA PEMILIHAN RUTE KOTA SANGATTA – SIMPANG PERDAU KM. 106 DESA TEPIAN INDAH PARENRENGI, ANDI
KURVA S JURNAL MAHASISWA Vol 11, No 2 (2020)
Publisher : KURVA S JURNAL MAHASISWA

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Abstract

Dalam melakukan perjalanaan dari daerah satu ke daerah lainnya biasanya dapat ditemukan beberapa rute yang bisa di lewati untuk sampai ke tujuan tersebut. Banyaknya pilihan jenis rute lalu lintas yang bisa ditempuh untuk menuju suatu daerah menuntut penguna jalan untuk memilh rute paling efektif, dengan maksud untuk mengefisiensikan jarak, waktu, dan biaya operasional yang dibutuhkan untuk mencapai tujuan tersebut. Simpang Perdau KM. 106 Desa Tepian Indah merupakan simpang yang menghubungkan beberapa kecamatan di Kabupaten Kutai Timur dan juga akses yang dilewati untuk melakukan perjalanan dari Kabupaten Kutai Timur menuju Kabupaten Berau ataupun sebaliknya, dari Kota Sangatta menuju Simpang Perdau KM. 106 Desa Tepian Indah kebanyakan masyarakat lebih memilih untuk melewati rute Jalan Poros Rantau Pulung dibandingkan melewati rute jalan Poros Perdau dikarenakan rute tersebut dianggap terpendek, padahal belum tentu rute tersebut optimal dari segi waktu tempuh maupun biaya operasional kendaraan. Metode yang digunakan untuk mengukur kecepatan pada penelitian adalah metode kecepatan bergerak (running speed) yaitu kecepatan rata-rata pada suatu jalur pada saat kendaraan bergerak, di dapat dari membagi panjang jalur dengan lama waktu kendaraan bergerak menempuh jarak tersebut tanpa menghitung waktu henti. Sedangkan untuk analisis biaya operasional menggunakan metode PCI (Pasific Consultant International). Dari hasil perhitungan dapat disimpulkan bahwa perjalanan dari Kota Sangatta menuju Simpang Perdau KM. 106 Desa Tepian Indah, rute yang paling efektif dari segi biaya operasional kendaraan adalah rute Jalan Poros Perdau dibandingkan dengan rute Jalan Poros Rantau Pulung walaupun rute tersebut memiliki selisih jarak tempuh kurang lebih 19,3 KM dimana rute Jalan Poros Rantau Pulung lebih pendek daripada rute Jalan Poros Perdau.
EVALUATING THE DIVERSITY AND ABUNDANCE OF MARINE FISH IN THE MAKASSAR STRAIT, INDONESIA THROUGH ENVIRONMENTAL DNA (EDNA) ANALYSIS Rukminasari, Nita; Hidayani, Andi Aliah; Moka, Wilma J.C.; Arbit, Nur Indah Sari; Andriyono, Sapto; Parenrengi, Andi
Jurnal Ilmu Kelautan SPERMONDE VOLUME 10 NUMBER 2, 2024
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jiks.v10i2.32405

Abstract

The recent adoption of environmental DNA (eDNA) represents an innovative method for assessing the presence of aquatic vertebrate species, providing a relatively straightforward approach with significant implications for conservation biology. In our investigation, we employed eDNA metabarcoding to explore the diversity of fish in the Makassar Strait. We collected eDNA from samples taken both at the surface and from the water column (15 m depth) at two specific locations within this region. The reliability of the MiFish-U primer set methodology in estimating fish diversity in the Makassar Strait was assessed. In a single survey, based on four water samples from the Makassar Strait, we successfully identified 11 marine fish taxa at the species level. These taxa belong to 8 families across 8 orders. The predominance of reef-dwelling species suggests that coral reefs play a dominant role as the primary ecosystem in this area. Among the surveyed sites, Barru Waters exhibited the highest species richness (7 species), while Pangkep Waters only revealed 4 species. Through the application of eDNA metabarcoding, this study provided a means to assess fish diversity, delivering crucial foundational information. Our findings highlight the cost-effectiveness of the eDNA metabarcoding method as a powerful scientific tool for the management and conservation of marine fish resources in the Makassar Strait.    
Assessing various administration strategies for dsRNA vaccine delivery: a concise review of VP15-WSSV research progress in tiger shrimp Penaeus monodon Parenrengi, Andi; Lante, Samuel; Suryati, Emma; Rosmiati, Rosmiati; Sulaeman, Sulaeman; Herlinah, Herlinah; Andriani, Yosie
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 3 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i3.73580

Abstract

Graphical Abstract     Highlight Research 1. The dsRNA application should correspond to the developmental stages of shrimp2. Immersion method was commonly used for larvae for handling many individuals3. Injection technique effectively delivered dsRNA to cells but was unsuitable for large-scale4. Oral administration of pellet-enriched dsRNA was applicable to apply on shrimp larvae, juveniles, and broodstocks     Abstract RNAi technology offers a novel powerful approach to silence gene expression by introducing double-stranded RNA (dsRNA) into the cell to degrade the mRNA at the post-transcriptional stage. An administration method of dsRNA delivery is one of the main considerations in applying the dsRNA vaccine for controlling pathogen infections. This mini-review was focused on the evaluation of three different methods (immersion, injection, and oral administration) of VP15-dsRNA vaccine delivery to the tiger shrimp post-WSSV challenge test. The immersion method was generally applied for the larval stage of tiger shrimp and seemed to be a simple technique for a large number of individuals in a small tank. The VP15-dsRNA application by immersion improved the survival of tiger shrimp larvae by 3.9% compared to the control groups. The injection technique was an effective way to deliver dsRNA to the cell, but it is difficult to apply in a large number of individuals or populations. The injection of VP15-dsRNA increased significantly the survival rate, proPO, and THC of tiger shrimp. A higher survival rate (75%) was exhibited in tiger shrimp injected with in vivo and in vitro VP15-dsRNA than in the control. Oral administration by pellet-enriched VP15-dsRNA was a useful way for larvae, juveniles, and broodstocks, but it has limitations since the pellet leaches into the water. The application of the VP15-dsRNA vaccine on the feed significantly enhanced the 26.7% higher survival rate compared to the control. The higher survival was also supported by a higher number of THC. The three VP15-dsRNA delivery methods provide potential approaches to increase tiger shrimp resistance to control pathogen infection
ANTI-BIOFOULING ACTIVITY OF SPONGE Callyspongia pseudoreticulata COMPONENTS EXTRACT AGAINST Balanus amphitrite Rosmiati, Rosmiati; Harlina, Harlina; Parenrengi, Andi; Suryati, Emma; Irmawati, Irmawati
Indonesian Aquaculture Journal Vol 19, No 2 (2024): (December, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.2.2024.147-156

Abstract

Biofouling attached to floating net cages and other aquaculture containers is an obstacle in aquaculture. The presence of biofouling can reduce the quality of the environment and interfere with the health of farmed animals, resulting in large losses. This study assessed the anti-biofouling activity of the Callyspongia pseudoreticulata extract component (EMCp) against the Barnacle Balanus amphitrite. For this purpose, C. pseudoreticulata was extracted with 80% methanol using the maceration method at <40°C to obtain EMCp. B. amphitrite was cultured in an aquarium at 25 ppt. The anti-biofouling activity of EMCp was tested against B. amphitrite larvae using asbestos plates. The study used a Complete Randomized Design with three treatments, namely, A) asbestos plate smeared with EMCp + varnish, B) asbestos plate smeared with varnish, and C) asbestos plate not smeared with EMCp and varnish (control). EMCp toxicity test on the larvae used clear bottles, and the adhesion test using 24-well polystyrene plates. Toxicity observations were done after 24 hours, and the number of dead larvae was calculated. The attachment of the larvae was calculated under a microscope at 10× magnification. Identification of groups of chemical compounds using a thin-layer chromatography chromatogram detected under UV lamps and spraying of reagents. The study found that EMCp effectively inhibited the attachment and growth of B. amphitrites with the LC50 of 150 mg/L. Studies of chemical constituents identified various compounds in the extract, including alkaloids, flavonoids, and terpenoids. These results suggest that C. pseudoreticulata has potential as a natural alternative to chemical-based antifouling agents.
SISTEM KALKULASI PENGUKURAN GROSS TONNAGE (GT) AND NET TONNAGE (NT) MENGGUNAKAN TEKNIK MODELING PADA KAPAL IKAN TRADISIONAL Rahman, Ariastuti; Kahpi, Ashabul; Muslihan, Muslihan; Parenrengi, Andi
JURNAL ILMU KOMPUTER Vol 11 No 1 (2025): Edisi April
Publisher : LPPM Universitas Al Asyariah Mandar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35329/jiik.v11i1.346

Abstract

Proses mengeluarkan surat ukur kapal nelayan tradisional masih dilakukan pengukuran Tonnage Kotor (GT) dan Tonnage Bersih (NT) secara manual oleh ahli ukur kapal sehingga kurang optimal dan efisien dalam proses perhitungan Tonnage Kotor dan Tonnage bersih sebuah kapal nelayan teradisional maka penulis mencoba membuat System Perhitunga Gross Tonnage / GT (Tonnage Kotor) dan Net Tonnage / NT (Tonnage Bersih) Pada Kapal Nelayan yang berfungsi menghitung Tonnage Kotor dan Tonnage Bersih secara otomatis setelah di lakukan pengukuran kapal sehingga ahli ukur kapal dapat mengetahui secara cepat serta akurat Tonnage Kotor dan Tonnage Bersih Kapal Nelayan tradisional. Tujuan penelitian ini adalah untuk merancang sebuah sistem pengukuran Gross Tonnage menggunakan Teknik Modeling yang di mana dapat mempermudah ahli ukur kapal dalam menentukan Gross Tonnage pada kapal sebagai syarat dalam menerbitkan sertifikat surat-surat Kapal. Pada Kantor Unit Penyelenggara Pelabuhan Kelas I Mamuju. Sehingga dapat mempermudah perhitungan GT kapal dengan menggunakan metode pendekatan modeling pada kapal ikan. Pengukuran GT kapal ikan dengan bantuan sebuah aplikasi yang akan menghasilkan nilai koefisien yang nantinya digunakan untuk menghitung GT kapal ikan sebagai syarat terbitnya SertifikatSurat –surat Kapal Nelayan. Hasil Penelitian Ini Adalah dalam Sistem Kalkulasi Pengukuran Gross Tonnage (GT) Dan Net Tonnage (NT) Pada Kapal Nelayan Berbasis Website menggunakan teknik Modeling” Menggunakan Bahasa Pemograman Php Dan Mysql, Hasil pengukuran dan dokumentasi yang dilakukan di lapang kemudian di gambar dengan menggunakan maxsurf untuk mengetahui nilai koefisiennya yang digunakan untuk mendapatkan nilai GT kapal. Nilai GT kapal yang didapatkan dari pengukuran langsung akan dibandingkan dengan nilai GT kapal yang ada disurat ukur kapal yang dimiliki oleh masing masing kapal ikan
Binary Vector Construction and Agrobacterium tumefaciens-mediated Transformation of Lysozyme Gene in Seaweed Kappaphycus alvarezii handayani, Tri; Alimuddin, Alimuddin; Widyastuti, Utut; Suryati, Emma; Parenrengi, Andi
BIOTROPIA Vol. 21 No. 2 (2014): BIOTROPIA Vol. 21 No. 2 December 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2014.21.2.348

Abstract

Ice-ice disease is the biggest problem in the cultivation of seaweed Kappaphycus alvarezii. The disease is caused by bacterial infection and induced by drastic changes of water quality. Lysozyme has the ability to break down bacterial cell wall. The purpose of this research was to construct of a binary vector pMSH1-Lys carrying chicken Lysozyme (Lis) gene and introduce pMSH1-Lis on K. alvarezii. The binary vector expression was transformed into Agrobacterium tumefaciens LBA4404 by triparental mating. Thallus was inoculated with A. tumefaciens carrying pMSH1-Lis and then the transformed thallus was selected by adding 20 mg/L hygromycin to the culture medium. PCR analysis showed that the construction of the binary plasmid pMSH1-Lys was established. Percentage of transformation of pMSH1-Lis on K. alvarezii was 23.56%, while the efficiency of regeneration was 11.32%. PCR analysis showed that three of the regenerated thallus contained Lysozyme gene. Thus, transgenic K. alvarezii was produced successfully and this can be useful for studying the mechanisms of seaweed defense against bacterial infection.
Co-Authors Andi Aliah Hidayani Andi Tenriulo Asphama, Andi Ivo Dody Dharmawan Trijuno, Dody Dharmawan Emma Suryati Harlina Harlina Herlinah Herlinah Irmawati Irmawati Kahpi, Ashabul Moka, Wilma J.C. Muslihan, Muslihan Nita Rukminasari Nur Indah Sari Arbit Rahman, Ariastuti Rosmiati Rosmiati Samuel Lante, Samuel Sapto Andriyono Sulaeman Sulaeman Tri Handayani UTUT WIDYASTUTI Yosie Andriani Yushinta Fujaya