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Anna Roosdiana
Chemistry Department, Brawijaya University Jalan Veteran, Malang
Chemical Engineering, Chemistry & Bioengineering Chemistry

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THE POTENTIOMETRIC UREA BIOSENSOR USING CHITOSAN MEMBRANE Ani Mulyasuryani; Anna Roosdiana; Arie Srihardyastutie
Indonesian Journal of Chemistry Vol 10, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.744 KB) | DOI: 10.22146/ijc.21454

Abstract

Potentiometric urea biosensor development is based on urea hydrolysis by urease resulted CO2. The biosensor is used chitosan membrane and the H3O+ electrode as a transducer. The research was studied of effecting pH and membrane thickness to the biosensor performance. The best biosensor performance resulted at pH = 7.3 and 0.2 mm of membrane thickness. The biosensor has a Nerntian factor 28.47 mV/decade; the concentration range is 0.1 up to 6.00 ppm; and the limit of detection is 0.073 ppm. The response time of this biosensor is 280 seconds, efficiency 32 samples and accuracy 94% up to 99%.
Co-Authors Ani Mulyasuryani Arie Srihardyastutie