THE POTENTIOMETRIC UREA BIOSENSOR USING CHITOSAN MEMBRANE

Ani Mulyasuryani (Chemistry Department, Brawijaya University Jalan Veteran, Malang)
Anna Roosdiana (Chemistry Department, Brawijaya University Jalan Veteran, Malang)
Arie Srihardyastutie (Chemistry Department, Brawijaya University Jalan Veteran, Malang)

Indonesian Journal of Chemistry
Vol 10, No 2 (2010)

Potentiometric urea biosensor development is based on urea hydrolysis by urease resulted CO2. The biosensor is used chitosan membrane and the H3O+ electrode as a transducer. The research was studied of effecting pH and membrane thickness to the biosensor performance. The best biosensor performance resulted at pH = 7.3 and 0.2 mm of membrane thickness. The biosensor has a Nerntian factor 28.47 mV/decade; the concentration range is 0.1 up to 6.00 ppm; and the limit of detection is 0.073 ppm. The response time of this biosensor is 280 seconds, efficiency 32 samples and accuracy 94% up to 99%.