I Wayan Gede Sutadarma
Faculty of Medicine Udayana University, Denpasar

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EFFECTS OF HIGH-FAT DIET FEEDING ON LIPID PROFILE IN RATS (Rattus norvegicus) Prawitha Pinatih, Putu Teja; Dewi, Ni Nyoman Ayu; Sutadarma, I Wayan Gede
E-Jurnal Medika Udayana Vol 11 No 10 (2022): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2022.V11.i10.P04

Abstract

Penyakit kardiovaskular merupakan pembunuh utama di dunia. Dasar penyakit kardiovaskular adalah proses dislipidemia. Sehingga, diperlukan model dislipidemia untuk penelitian tentang terapi penyakit kardiovaskular. Tujuan penelitian ini adalah untuk meneliti efek dari pemberian diet tinggi lemak terhadap profil lipid tikus sebagai model dislipidemia. Pakan standar untuk tikus dicampurkan dengan 30% lemak babi dan 20% kuning telur untuk menghasilkan pakan tinggi lemak. Enam ekor tikus Wistar berumur tiga bulan dengan berat 200±10g dibagi menjadi dua kelompok perlakuan, 3 ekor diberi makan pakan standar (NF) dan 3 ekor diberi makan diet tinggi lemak (FF). Tikus diberi makan selama 6 minggu sesuai kelompok perlakuan. Sampel darah kemudian dianalisis untuk mengukur kadar kolesterol dengan metode CHOD-PAP, kadar trigliserida dengan metode GPO-PAP, dan kadar HDL dengan metode homogenous enzyme colorimetric assay. Kadar LDL dihitung menggunakan rumus Friedewald. Hasil analisis sebagai berikut. Kolesterol total: 55.00 mg/dL pada kelompok NF; 92.67 mg/dL pada kelompok FF (p=0.027). Trigliserida: 78.33 mg/dL pada kelompok NF; 171.67 mg/dL pada kelompok FF (p=0.014). HDL: 32.00 mg/dL pada kelompok NF; 43.00 mg/dL pada kelompok FF (p=0.422). LDL: 7.33 mg/dL pada kelompok NF; 15.33 mg/dL pada kelompok FF (p=0.437). Hasil penelitian ini menunjukkan peningkatan signifikan pada kolesterol total dan trigliserida namun tidak ada perbedaan bermakna pada kolesterol HDL dan LDL; komposisi diet pada penelitian ini belum sesuai untuk studi dislipidemia yang diarahkan sebagai model aterosklerosis. Kata Kunci: diet tinggi lemak., dyslipidemia., profil lipid
PENGARUH PERBEDAAN KONSENTRASI MADU KELE TERHADAPaPERTUMBUHAN BAKTERIaMETHICILLINaRESISTANTaSTAPHYLOCOCCUSaAUREUSa(MRSA) Ekaputra, Ariel; Wiryanthini, Ida Ayu Dewi; Sutadarma, I Wayan Gede
E-Jurnal Medika Udayana Vol 11 No 6 (2022): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2022.V11.i06.P03

Abstract

Luka adalah tempat dimana terputusnya kontinuitas dari jaringan kulit dan jaringan dalam,ediskontinuitas ini menjadi jalur masuk bagi mikroorganisme patogen sehingga dapat menyebabkan infeksi. Infeksiadarialuka seringkali disebabkan oleh bakteriaStaphylococcus Aureus. Seiring dengan penggunaan antibiotik yang berlebihan maka kemudian terjadinya resistensi, kemudian muncul suatu strain baru yaitu Methicillin Resistant Staphylococcus Aureus. Tujuan penelitian ini adalah untuk mengetahui apakah Madu Kele Sanjiwani dapat menghambat pertumbuhan Methicillin Resistant Staphylococcus Aureus dan untuk mengetahui apakah terdapat perbedaan daya antibakteri Madu Kele Sanjiwani dengan konsentrasi 12,5%, 25%, 50%, 100% terhadap pertumbuhan Methicillin Resistant Staphylococcus Aureus. Madu seringkali digunakan dalam pengobatan sebagai bahan pembalut luka, luka bakar. Madu digunakan sebagai pembalut luka karena dipercaya memiliki beberapa sifat yang mendukung fungsinya yaitu sifat antibakteri, anti inflamasi, dan antioksidan. Sifat antibakteri dikaitkan dengan kadar gula yang tinggi, tingkat pH yang rendah, dan kandungan Hidrogen Peroksidanya. Madu Kele Sanjiwani yang merupakan madu yang dihasilkan di Bali, Indonesia digunakan sebagai sampel pada studi ini. Sebelumnya belum ada penelitian lain yang menggunakan Madu Kele Sanjiwani dan mencari tahu mengenai efek antibakterinya. Madu Kele Sanjiwani kemudian diencerkan menjadi 4 kelompok konsentrasi yaituk12,5%, 25%, 50%, dank100%, dilanjutkan uji aktivitas antibakteri menggunakan metode disk diffusion. Hasil Penelitian menunjukan konsentrasi 100% menghasilkan zona hambat dengan rerata diameter 7mm sedangkan kelompok konsentrasi lainnya tidak memiliki zona hambat. Dapat disimpulkan bahwa hanya Madu Kele Sanjiwani dengan konsentrasi 100% yang mampu menghambat pertumbuhan bakteri Methicillin Resistant Staphylococcus Aureus. Kata Kunci: Madu Kele., antibakteri., MethicilliniResistantiStaphylococcusiAureusi(MRSA)
THE RELATIONSHIP BETWEEN SIMPLE SUGAR INTAKE AND THE ABDOMINAL CIRCUMFERENCE OF PSSK STUDENTS Sharma, Ansehs; SUTADARMA, I WAYAN GEDE; SURUDARMA, I WAYAN; WIRYANTHINI, IDA AYU DEWI
E-Jurnal Medika Udayana Vol 13 No 9 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i09.P06

Abstract

The abdominal circumference is dependent upon various factors, one of it is the simple sugar intake. Hence, research has been conducted in order to determine the effects of simple sugar intake on the abdominal circumference because there is an increase in people who have a large abdominal circumference in the past years, mainly due to increase in simple sugar intake. The research was conducted using a descriptive design with a cross-sectional analytical research method amongst the Medical Students of Udayana University by providing questionnaires. For the majority of the students, the simple sugar intake appears to be in the mid-range. There are 15 students, whose intake is more than the mid-range and 14 student whose intake below the mid-range. For majority of students, the abdominal circumference appears to be around the mid-range, 65.8cm to 92.8cm. There are 8 students whose abdominal circumference is more than the mid-range and another 8 students whose abdominal circumference is below the mid-range. The Pearson chi-square test value is 25.772 with an asymptotic significance 2 tailed (often referred to as “p”, short for probability) value of 0.018. Since this p-value is below 0.05, it means there is a significant or valid relationship between the two variables as expected.In conclusion, there is a significant relationship and moderate correlation between the intake of simple sugars and abdominal circumference amongst the PSSK UNUD students
DETEKSI BAKTERI ENTEROHEMORRHAGIC ESCHERICHIA COLI PADA LAWAR MERAH BABI DENGAN MENGGUNAKAN TEKNIK POLYMERASE CHAIN REACTION (PCR) PADA WARUNG DI DENPASAR Tjoantara, Andien Nikita; Surudarma, I Wayan; Wiryanthini, Ida Ayu Dewi; Sutadarma, I Wayan Gede
E-Jurnal Medika Udayana Vol 12 No 5 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i05.P08

Abstract

Lawar merah babi is one of the typical Balinese dish made from pork, pork skin, some vegetables, coconut, various spices, and fresh blood as red colour. The processing of lawar still very traditional. In the process of processing and serving lawar merah babi, the sellers pay less attention to hygiene such as the materials that contaminated with bacteria, personal hygiene, and unclean cooking equipment. If it is not processed properly and hygienically, it is likely to be contaminated with Escherichia coli bacteria which if consumed will be pathogenic to cause diarrhea. This study aims to determine the presence of Escherichia coli bacterial contamination, especially the Enterohemorrhagic Escherichia coli pathotype on lawar merah babi that sold in stalls in Denpasar with the PCR technique. This research is a descriptive study with a cross sectional research design. The sampling technique used cluster purposive sampling technique with a total sample of 13 samples taken at stalls in Denpasar. All samples were examined at the Integrated Biomedical Laboratory, Department of Biochemistry, Faculty of Medicine, Udayana University. The research procedure starts from lawar merah babi was isolated for protein with a 0.6 probe and then DNA was isolated using a DNA isolation kit (cell lysis solution, nuclear lysis solution, protein precipitation solution, DNA rehydration solution). After that, EHEC was detected using stx-1 and stx-2 as target genes using PCR technique. Of the 13 samples studied, the results obtained did not detect any EHEC pathotype in all samples of the lawar merah babi.
HUBUNGAN ASUPAN JENIS BERAS TERHADAP KADAR GLUKOSA DARAH SEWAKTU PADA PASIEN DIABETES MELLITUS TIPE II Utami Dewi, Ayu Dian; Sutadarma, I Wayan Gede; Wiryanthini, Ida Ayu Dewi
E-Jurnal Medika Udayana Vol 11 No 5 (2022): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2022.V11.i05.P05

Abstract

Type II DM is a metabolic disease characterized by increase blood glucose level is caused secretion insulin dysfunction and insulin resistance. An increase in the incidence of DM can occur because of an unbalanced diet. Aim this study to determine the relationship of rice type intake with random blood glucose levels in type II DM patients. This study was a cross sectional analytic study which was conducted from September until November 2020 at the Puskesmas Denpasar Barat I and II. The sample in this study were patients with type II DM who met the inclusion criteria is a patient with a consuming rice, and a patient without using insulin. Exclusion criteria were a patient without consuming rice. Samples were obtained using simple random sampling technique and obtained 31 samples that met the inclusion criteria. The study was conducted by asking the sample for informed consent and asking sample to fill out an online questionnaire that contain food intake that the sample consumes in a day. Normality test is using Saphiro-wilk test and bivariate analysis with Rank-Spearman test. White rice type intake was 61,3%, red rice type intake was 35,5% and black rice type was 3,2%. Carbohydrate of rice intake in women amounted to 95,281 grams and in men 101, 810 grams. Calorie intake per day for women was 1226,0 kcal and in men was 1300,6 kcal. From analysis result there is a relationship between rice type intake, calorie, and carbohydrate with random blood glucose level (p= 0,000, p= 0,000, p= 0,000). Strength of correlation (r= 0,717, r= 0,945, r= 0,945). There is a significant relationship between rice type intake on random blood glucose level with a strong correlation.
Potential of Purple Sweet Potato Ethanol Extract (Ipomea Batata L.) to Prevent Skin Aging in Menopausal Wistar Rats Wiryanthini, Ida Ayu Dewi; Sutadarma, I Wayan Gede; Dewi, Ni Putu Ratih Cahaya; Aryaweda, Made Dhiyo Wiweka
Review of Primary Care Practice and Education Vol 8, No 1 (2025): January
Publisher : Faculty of Medicine, Public Health, and Nursing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/rpcpe.103175

Abstract

Background: Menopause is a hypo-estrogenic condition due to decreased ovarian function, a natural thing experienced by women between the ages of 45 and 55, causing health problems on the skin, in the form of decreased collagen production so that skin elasticity decreases, and is prone to dry conditions, sagging to fine lines and wrinkles as a sign of skin aging also called Estrogen Deficient Skin (EDS). This study aims to determine the role of anthocyanin activity in purple sweet potatoes as phytoestrogens to overcome skin symptoms caused by EDS in the form of proliferation of collagen-forming cells, namely fibroblast cells in Wistar rats with a menopause model. Method: This research is purely experimental with the method Post Test Only Control Group Design which aims to determine the effect of administering purple sweet potato ethanol extract on the number of fibroblast cells in the skin tissue of Wistar rats with a menopause model. A total of 16 female Wistar rats aged 12 weeks that were bilaterally ovariectomized were divided into 2 groups, namely group P0 given 1 ml/day of distilled water, group P1 given ethanol extract of purple sweet potato at a dose of 400 mg/kg BW/day for 3 months. On the last day of treatment, the experimental animals were terminated and then skin tissue was taken to make histological preparations of Masson's Trichrome and to examine the number of fibroblast cells. Results: Data analysis using the Independent T test obtained significant results p=0.00 and Post Hoc LSD obtained significant results p<0.05 for all comparisons across groups. Conclusion: Based on these results, it can be concluded that Anthocyanin in ethanol extract of purple sweet potato can increase fibroblast cell proliferation in skin tissue of Wistar rats with a menopause model. Further research is still needed on other parameters to prove the role of Anthocyanin as a phytoestrogen to overcome EDS.