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All Journal Dentino: Jurnal Kedokteran Gigi Oceana Biomedicina Journal SONDE (Sound of Dentistry) Denta: Jurnal Kedokteran Gigi Jurnal of Syiah Kuala Dentistry Society
Vinna Kurniawati Sugiaman
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimum (KBM) Ekstrak Etanol Daun Ketepeng Cina (Cassia alata L.) Terhadap Pertumbuhan Candida albicans Yassie Shailla Gharnita; Shelly Lelyana; Vinna Kurniawati Sugiaman
SONDE (Sound of Dentistry) Vol. 4 No. 1 (2019): SONDE (Sound of Dentistry)
Publisher : Maranatha Christian University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.022 KB) | DOI: 10.28932/sod.v4i1.1766

Abstract

Candida albicans merupakan jamur dimorfik oportunistik penyebab utama oral candidiasis. Salah satu obat oral candidiasis yang suing digunakan adalah nistatin namun memiliki banyak efek samping maka dibutuhkan pengobatan alternatif dengan memanfaatkan tanaman herbal seperti daun ketepeng cina (Cassia alata L.). Penelitian ini bertujuan untuk mengetahui kadar hambat minimum (KHM) dan kadar bunuh minimum (KBM) ekstrak etanol daun ketepeng cina (Cassia alata L.) terhadap pertumbuhan Candida albicans dengan menghitung jumlah yeast. Penelitian ini bersifat eksperimental laboratorium dengan membandingkankelompok uji yang mengandung ekstrak daun ketepeng cina (Cassia alata L.) dengan konsentrasi 200 mg/mL; 100 mg/mL; 50 mg/mL; 25 mg/mL; 12,5 mg/mL; 6,25 mg/mL dan 3,125 mg/mL yang ditanam dalam media PDA. Kontrol positif berupa nistanin, dan media PDB sedangkan kontrol negatif berupa media PDB, dan DMSO 10% terhadap Candida albicans yang sebelumnya telah diinkubasi pada suhu 37oC selama 24 jam dan dilakukan pengulangan sebanyak empat kali. Berdasarkan basil penelitian diketahui bahwa ekstrak etanol daun ketepeng cina (Cassia alata L.) mampu menghambat pertumbuhan Candida albicans pada konsentrasi 200 mg/mL secara sig (p<0,05) namun tidak dapat membunuh Candida albicans. Simpulan dari penelitian ini menunjukkan bahwa ekstrak etanol daun ketepeng cina (Cassia alata L.) dapat menghambat pertumbuhan Candida albicans.
Peranan Sel Punca dalam Meregenerasi Jaringan Pulpa Gigi Vinna Kurniawati Sugiaman
SONDE (Sound of Dentistry) Vol. 4 No. 1 (2019): SONDE (Sound of Dentistry)
Publisher : Maranatha Christian University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.293 KB) | DOI: 10.28932/sod.v4i1.1770

Abstract

Injury pada pulpa gigi dapat menyebabkan terjadinya perubahan pada pulpa secara histopatologi, yang apabila terus berlanjut akan menyebabkan kerusakan pulpa menjadi irreversible sampai dengan kematian yang pada akhirnya perlu untuk dilakukan perawatan endodontik. Perawatan ini dapat mempertahankan gigi di rongga mulut dalam kondisi non vital, sehingga belakangan ini dikembangkan suatu teknologi baru yaitu teknologi tissue engineering dengan memanfaatkan sel punca, matriks, dan growth factor untuk meregenerasi jaringan yang mengalami kerusakan. Pada proses regenerasi, sel punca akan mengalami proliferasi dan diferensiasi menjadi berbagai macam sel yang terdapat pada jaringan pulpa, sehingga proses regenerasi jaringan pulpa yang mengalami kerusakan dapat terjadi dan pulpa dapat tetap dalam kondisi vital bertahan di rongga mulut.
Potensi Herbal Antibakteri Cuka Sari Apel terhadap Enterococcus faecalis sebagai Bahan Irigasi Saluran Akar Rudy Djuanda; Varin Aulia Helmika; Fiona Christabella; Natallia Pranata; Vinna Kurniawati Sugiaman
SONDE (Sound of Dentistry) Vol. 4 No. 2 (2019): SONDE (Sound of Dentistry)
Publisher : Maranatha Christian University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28932/sod.v4i2.2141

Abstract

Irigasi saluran akar merupakan tahapan penting dalam menunjang keberhasilan perawatan saluran akar. Mikroorganisme paling resisten dan sering ditemukan pada kasus setelah dilakukan perawatan saluran akar adalah Enterococcus faecalis, dengan prevalensi berkisar 24-77%. Chlorhexidine digluconate dengan konsentrasi 2% digunakan untuk larutan irigasi saluran akar yang efektif, namun tidak memiliki kemampuan untuk melarutkan jaringan nekrotik. Cuka sari apel memiliki kandungan asam organik yaitu asam asetat dapat yang dapat bertindak sebagai antimikroba yang dapat menyebabkan hilangnya integritas sel. Penelitian ini menguji pengaruh antibakteri, daya hambat, kadar hambat minimum, dan kadar bunuh minimum sediaan cuka sari apel terhadap bakteri Enterococcus faecalis dengan Chlorhexidine digluconate 2% sebagai kelompok kontrol terhadap pertumbuhan Enterococcus faecalis. Hasil penelitian ini diharapkan dapat menjadi suatu dasar pengembangan cuka sari apel sebagai bahan irigasi saluran akar dan dapat mengetahui aktivitas daya hambat optimum dari sediaan cuka sari apel terhadap bakteri Enterococcus faecalis secara in vitro.Desain penelitian ini bersifat eksperimental labotratorik secara in vitro menggunakan metode difusi cakram (Tes Kirby-Bauer) dengan pengamatan zonahambat. Data yang diukur adalah diameter zona hambat dari pertumbuhan bakteri Enterococcus faecalis dengan menggunakan jangka sorong dalam satuan milimeter (mm). Kadar bunuh bakteri pada penelitian ini diadaptasi dari metode yang dikembangkan oleh CLSI (Clinical Laboratory Standart Institute, 2014) dengan modifikasi.Diameter zona hambat cuka apel meningkat seiring dengan peningkatan konsentrasi, bahkan pada konsentrasi minimal 25% dapat membunuh bakteriEnterococcus faecalis Potensi antibakteri cuka apel setara dengan Chlorhexidine digluconate 2%.
PENGARUH OBAT KUMUR HERBAL DENGAN KANDUNGAN ZAT AKTIF FLAVONOID, SAPONIN, DAN TANIN TERHADAP HALITOSIS (STUDI LITERATUR) Eddea Marselyna A.D.; Riani Setiadhi; Vinna Kurniawati Sugiaman
Oceana Biomedicina Journal Vol 5 No 2 (2022): Oceana Biomedicina Journal
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/obj.v5i2.29

Abstract

Introduction: Herbal mouthwash has been developed for its antibacterial properties. Halitosis is bad breath that comes from the mouth. Volatile sulfur compounds (VSCs) are the result of oral bacterial activity production, by Porphyromonas gingivalis which is an gram-negative anaerobic bacteria causing halitosis. Flavonoids have the potential to be an antioxidant and have antibacterial, anti-inflammatory, anti-allergic and anti-thrombotic activities, while saponins and tannins are a group of active plant compounds that are phenolic, have a septic taste and antibacterial activity. The aim of this literature study was to improve knowledge about the effect of herbal mouthwashes with active substances of flavonoids, saponins and tannins on halitosis. Methods: Literature review using research results, articles and other literatures related to herbal mouthwash and medicinal plant content against halitosis. Results: Some researchers had shown that herbal mouthwashes with antibacterial content in the form of flavonoids, saponins and tannins could inhibit the growth of oral bacteria such as Streptococcus mutans, Porphyromonas gingivalis and Streptococcus sanguinis, which would inhibit plaque formation, reducing halitosis, overcoming gingivitis, increasing salivary pH and reducing salivary viscosity. Conclusion: Based on the search results of the search, it can be concluded that herbal mouthwash containing active substances of flavonoids, saponins and tannins has an effect in overcoming halitosis.
EFFECTIVENESS OF TAMARIND LEAF (Tamarindus indica L.) ETHANOL EXTRACT ANTIBACTERIAL AGAINST Porphyromonas gingivalis Yemima Putri Wijaya Baiin; Henry Y. Mandalas; Vinna Kurniawati Sugiaman
Dentino : Jurnal Kedokteran Gigi Vol 8, No 1 (2023)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v8i1.16089

Abstract

ABSTRACTBackground: One of the initial measures to prevent chronic periodontitis is the use of mouthwash. Chlorhexidine is an antibacterial agent that can be used as a mouthwash. Chlorhexidine has side effects so that a new method is needed to prevent periodontitis with fewer side effects, namely by using plants as a medicine, one of the plants containing active compounds with antibacterial effects. The ethanol extract of tamarind leaf is proven to contain active compounds of flavonoids, tannins, alkaloids, and saponins.  Purpose: The aim of the study was to analyze the antibacterial effect of ethanol extract of tamarind leaf (Tamarindus indica L.) leaf against Porphyromonas gingivalis bacteria. Material and methods: The research is an experimental laboratory type using the disc diffusion method (Kirby-Bauer), namely the paper disc diffusion method with the test material of ethanol extract of tamarind leaf (Tamarindus indica L.) in various concentrations, namely: 3.125%, 6.25%, 12.5 %, 25%, 50%, and 100%. Results: Based on the results of the research on the antibacterial effect of the ethanolic extract of tamarind leaf with concentrations of 3.125%, 6.25%, and 12.5% with an average diameter of 0.00 mm of inhibition zone. At concentrations of 25%, 50%, and 100%, they fall into the medium criteria group, because they have an average inhibition zone diameter of 5.21 mm, 7.45, and 9.16 mm, so that the concentration of 100% is closest to the average the mean diameter of the positive control group or chlorhexidine. Conclusion: It can be concluded that the ethanol extract of tamarind leaf (Tamarindus indica L.) has an antibacterial effect that can inhibit the growth of Porphyromonas gingivalis bacteria at a concentration of 100% with an average inhibition zone diameter of 9.16 mm. Keywords: Antibacterial,   Porphyromonas gingivalis, Tamarind leaf
Comparative Effectiveness of Mouthwash Containing Chlorhexidine 0.12% and Cetylpyridinium Chloride 0.1% with Chlorhexidine 0.2% against Porphyromonas gingivalis Karissa Adiela Sutanto; Vinna Kurniawati Sugiaman; Henry Yonatan Mandalas
Denta Journal Kedokteran Gigi Vol 19 No 1 (2025): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v19i1.3

Abstract

Background: Periodontitis is an inflammatory disease that affects the supporting tissue of teeth and causes damage to the periodontal ligament and alveolar bone, generally caused by microorganisms. Chronic periodontitis is often caused by gram-negative anaerobic bacteria, especially Porphyromonas gingivalis. Mouthwash active ingredients that frequently used such as chlorhexidine (CHX) or cetylpyridinium chloride (CPC) have an antibacterial effect and prevent plaque formation. Objective: This research aims to compare the effectiveness of mouthwash containing CHX 0.12% and CPC 0.1% with CPC 0.2% on the bacterium Porphyromonas gingivalis ATCC 33277. Materials and Methods: This research is an experimental laboratory by giving treatment to Porphyromonas gingivalis with mouthwash CHX 0.12% and CPC 0.1%, CHX 0.2%, and distilled water as the negative control, then calculating the inhibition zone for each treatment and continued with One-Way ANOVA test. Results: The result showed the largest average of the inhibitory zone diameter was on CHX 0.2% which was 12,7 mm. Meanwhile, mouthwash containing CHX 0.12% and CPC 0.1% showed an average diameter of inhibitory zone resulted in 10,97 mm. Conclusion: In conclusion, CHX 0.2% has greater ability of inhibitory than mouthwash containing CHX 0.12% and CPC 0.1% against the growth of Porphyromonas gingivalis.
Antibacterial potential ethanol extract of beluntas leaves (Pluchea indica L) to Streptococcus sanguinis Wilma ADIWIJAYA; Riani SETIADHI; Vinna Kurniawati SUGIAMAN
Journal of Syiah Kuala Dentistry Society Vol 7, No 2 (2022): December 2022
Publisher : Dentistry Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jds.v7i2.30230

Abstract

Recurrent aphthous stomatitis (RAS) is one of the most common oral diseases in the community, with a prevalence of 5-66%, with one of the predisposing factors being Streptococcus sanguinis. Treatment for RAS has been symptomatic and supportive, including antiseptic mouthwash such as chlorhexidine gluconate 0.2% or topical corticosteroids (triamcinolone acetonide 0.1% in Orabase). However, these drugs have some side effects. Treating herbal ingredients such as Beluntas leaves low prices and minimal side effects. The active compounds in Beluntas leaves are phenols, tannins, flavonoids, saponins, triterpenoids, essential oils, terpenoids, and many compounds known to have antibacterial activity. Methods: This study aimed to determine the minimum inhibitory level (MIC), and minimum killing rate (MBC) of 96% ethanol extract of Beluntas leaves on the growth of Streptococcus sanguinis. MIC was measured by broth microdilution technique with DMSO solvent 10% and eight concentrations of beluntas extract. Chlorhexidine gluconate 0.2% was used as a positive control for the comparison compound. Furthermore, the MBC test was carried out using the total plate count method for treatments that gave the MIC value. One Way Anova analysis with Post Hoc Tukey was used to determine the significant difference between treatments. Results: The ethanol extract of Beluntas leaves (Pluchea indica L) has a Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for the growth of Streptococcus sanguinis with a minimum inhibitory concentration of 3.95 g/mL and a minimum concentration of 7.8 g/mL. Conclusion: The ethanol extract of Beluntas leaves (Pluchea indica L) has the potential as an antibacterial against Streptococcus sanguinis. KEYWORDS: Recurrent aphthous stomatitis, Streptococcus sanguinis, ethanol extract of beluntas leaves, Minimum Inhibitory Concentration, Minimum Bactericidal Concentration
Pengaruh Pelapisan yang Dapat Dimakan terhadap Perubahan Warna dan Sifat Mekanik Resin Akrilik yang Disembuhkan dengan Panas yang Direndam dalam Teh Hitam (Camellia Sinensis) Hernindya Dwifulqi; Silvia Nailani; Vinna Kurniawati Sugiaman; Theodora Adhisty Dwiarie; Maria Florensia Rosa Centifolia Bulata Dolu
Denta Journal Kedokteran Gigi Vol 19 No 2 (2025): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v19i2.3

Abstract

Background: Dentures are a standard treatment option for replacing missing teeth. One of the most essential components of dentures is the denture base. However, the acrylic resin has a weakness, namely that it has porosity, which can cause water absorption and colour changes. Water absorption in the acrylic resin can affect its colour stability, flexural strength, and hardness over a specific period. Tea is a drink often consumed in Indonesia. Black tea has undergone oxidation of several polyphenolic compounds (catechins). The fermentation process results in the oxidation of simple polyphenols, where tea catechins are converted into more complex and concentrated molecules that give black tea its characteristic colour. The edible coating is a thin layer made from consumable materials that acts as a barrier. Objective: To determine the effect of edible coating on heat-cured acrylic resin denture plates on colour retention, flexural strength, and hardness after soaking in black tea. Materials and Methods: This research utilized 25 heat-cured acrylic resin plates, divided into five groups with varying soaking times for the edible coating. The plates were then soaked in black tea for 15 hours, after which testing was conducted on the color resistance, hardness, and flexural strength of the heat-cured acrylic resin plates. Results: Acrylic resin plates with chitosan have better colour retention than acrylic resin not soaked with chitosan. Meanwhile, flexural strength and hardness values between groups did not differ significantly. Conclusion: Chitosan immersion affects the colour durability of the heat-cured acrylic resin denture base without significantly decreasing the hardness and strength values.
Co-Authors Djuanda, Rudy Dwiarie, Theodora Adhisty Eddea Marselyna A.D. Fiona Christabella Henry Y. Mandalas Henry Yonatan Mandalas Hernindya Dwifulqi Karissa Adiela Sutanto Lelyana, Shelly Maria Florensia Rosa Centifolia Bulata Dolu Natallia Pranata Riani Setiadhi Silvia Nailani Varin Aulia Helmika Wilma ADIWIJAYA Yassie Shailla Gharnita Yemima Putri Wijaya Baiin