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All Journal JURNAL ATOMIK
Djihan Ryn Pratiwi
Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science

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SCREENING LIPASE FROM ENDOPHYTIC BACTERIA FROM STEAM OF (Costus speciosus (J.Koenig) Sm) AND OPTIMUM WORKING CONDITION Asjayani Kurnia Sari; Winni Astuti; Djihan Ryn Pratiwi
JURNAL ATOMIK Vol 5 No 1 (2020)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Endophytic bacteria are bacteria that live in plant tissues such as the roots, leaves, and stems of plants. This research was conducted to screen lipase-producing endophytic bacteria from Pacing stem (Costus speciosus (J.Koenig) Sm.) and determine the optimum working conditions. Screening of lipase-producing bacteria on nutrient agar media containing olive oil and Rhodamine B showed that one isolate was able to produce lipase. The crude lipase extract produced from endophytic bacteria worked optimally at a pH of 7, the temperature of 40℃, and a substrate concentration of 2%. Keywords : Costus speciosus (J.Koenig) Sm., Endophytic bacteria, Lipase, Optimum Activity
SELEKSI AMILASE PENDEGRADASI PATI MENTAH BAKTERI DARI AIR GALIAN BEKAS TAMBANG BATU BARA DI DESA LOA BAHU Akbar Akbar; Winni Astuti; Djihan Ryn Pratiwi
JURNAL ATOMIK Vol 10 No 2 (2025)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v10i2.1546

Abstract

Raw starch-degrading amylase (RSDA) is a type of hydrolase enzyme that can degrade raw starch into glucose without going through the heating and gelatinization process. This study was conducted to select RSDA-producing bacteria from ex-coal mine water samples in Loa Bahu Village, Sungai Kunjang, Samarinda, with various types of raw starch such as rice, wheat, corn, potatoes, sago, and cassava. The selection of bacteria-producing RSDA enzymes was carried out using agar nutrient media containing raw starch, the positive test was marked by the formation of a clear zone around the bacterial colony after the addition of iodine solution. A total of 13 bacterial isolates (AT 1 - AT 13) were tested qualitatively by RSDA. The selection results showed that 4 bacterial isolates were capable of degrading raw rice starch, 4 bacterial isolates were capable of degrading raw corn starch, 2 bacterial isolate was capable of degrading raw cassava starch, 1 bacterial isolate was capable of degrading raw potato starch, 1 isolate was capable of degrading raw wheat starch, and 2 bacterial isolates were able to degrade raw sago starch. The bacterial isolate with the code AT3 is capable of degrading raw starch of rice, corn, potatoes, wheat and sago.
Co-Authors Akbar Akbar Asjayani Kurnia Sari Winni Astuti