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Gambaran Methicillin Resistant Staphylococcus aureus (MRSA) Pada Petugas Kesehatan RSUD Wangaya Kota Denpasar Ida Bagus Oka Suyasa
Meditory : The Journal of Medical Laboratory Vol 8, No 1 (2020): Meditory, vol. 8 no 1 Juni 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Background. Methicillin Resistant Staphylococcus aureus (MRSA) is a Staphylococcus aureus bacterium that is immune to penicillin isoxazoyl antibiotics such as methicillin, oxacillin and flucloxacillin. Staphylococcus aureus is one of the normal flora of the anterior nares and skin, which can be pathogenic when the patient's immune condition is deteriorating.Purpose. to determine the percentage of Methycillin Resistant Staphylococcus aureus (MRSA) in health staff at Wangaya District Hospital in Denpasar.Method. type of research is descriptive research design with Cross Sectional. The results of nasal swabs were implanted on MSA media and then catalase and coagulase tests were performed. The methicillin resistance test is carried out if after a positive catalase test and a positive coagulase test. If found MRSA will be continued by sensitivity test with antibiotics based on CLSI.Results. found five isolates of Staphylococcus aureus (11.36%), one MRSA isolate (2.27%), sensitive antibiotics were Tetracyclin, Cephazolin, Trimethroprim, Amikacin, Ciprofloxacin, Doxycycline, Ofloxacine and Levofloxacine.Conclusion. One MRSA isolate (2.27%) founded from nasal swabs of health staff at Wangaya District Hospital in Denpasar.

Penyuluhan Kesehatan serta Pemeriksaan Kadar Hemoglobin dan Protein Urin pada Remaja Putri Heri Setiyo Bekti; I Gusti Agung Ayu Dharmawati; Ida Bagus Oka Suyasa
Poltekita: Jurnal Pengabdian Masyarakat Vol. 4 No. 1 (2023): Januari-Maret
Publisher : Pusat Penelitian & Pengabdian Masyarakat Poltekkes Kemenkes Palu

Adolescence is an age stage that comes after childhood ends which is characterized by rapid growth with an age range of 10-19 years. Teenage girls are generally more prone to anemia than teenage boys. This community service activity aims to provide education to teenage girls about anemia and laboratory tests such as Hb levels and urine protein as an effort to provide health services to teenage girls at SMPN 1 Ubud, Gianyar. The method of implementing this activity includes counseling about anemia, checking Hb levels, and urine protein examination, as well as pre-test and post-test to determine the level of knowledge of teenage girls about anemia. From the results of the examination of 34 respondents, it was found that 29.4% had anemia and none of the respondents had proteinuria. As many as 94% of respondents have a good level of knowledge about anemia. There needs to be a role from family, school, and government to improve the health status of teenage girls such as giving blood tablets regularly to avoid anemia.

PENYULUHAN FAKTOR RISIKO PENYAKIT KARDIOVASKULER (PKV) DAN PEMERIKSAAN KADAR KOLESTEROL PADA GURU SD DI KECAMATAN SUKAWATI GIANYAR I Gusti Agung Dewi Sarihati; Cok Dewi Widhya HS; I Gusti Ayu Sri Dhyanaputri; Heri Setiyo Bekti; Ida Bagus Oka Suyasa
Jurnal Pengabmas Masyarakat Sehat Vol 2, No 2 (2020): April 2020
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/ms.v2i2.997

ABSTRACTHigh cholesterol increases the likelihood of the deposition of excess cholesterol in the walls of blood vessels and forming plaque, resulting in narrowing of the lumen and if left untreated can clog arteries and lead to cardiovascular disease. To prevent this, it can be done following a healthy lifestyle as endeavored by the Ministry of Health of the Republic of Indonesia. This community service aims to increase public knowledge and provide cholesterol check services in the prevention of cardiovascular disease that is carried out in Sukawati I District, Gianyar. The results showed that before counseling 5.4% of respondents answered 75% or more of the questions correctly. After being given an explanation accompanied by a leaflet, the respondent's knowledge became marked by an increase in the ability to answer that is 97.3% able to answer questions above 75%. Respodents with cholesterol levels above normal are most often found in respondents who have less knowledge that is 50%, the rest are found in respondents with sufficient and good knowledge. Percentage of respondents with normal cholesterol levels is most often found in respondents who have sufficient knowledge that is 74%. From these data it can be concluded that the total cholesterol level of respondents above normal is most often found in respondents with less knowledge. Thus, it is necessary to do continuous counseling to be able to change behavior towards supporting the prevention of cardiovascular disease.

PENYULUHAN FAKTOR RISIKO PENYAKIT KARDIOVASKULER (PKV) DAN PEMERIKSAAN KADAR KOLESTEROL PADA GURU SD DI KECAMATAN SUKAWATI GIANYAR I Gusti Agung Dewi Sarihati; Cok Dewi Widhya HS; I Gusti Ayu Sri Dhyanaputri; Heri Setiyo Bekti; Ida Bagus Oka Suyasa
Jurnal Pengabmas Masyarakat Sehat Vol 2, No 2 (2020): April 2020
Publisher : Poltekkes Kemenkes Denpasar

ABSTRACTHigh cholesterol increases the likelihood of the deposition of excess cholesterol in the walls of blood vessels and forming plaque, resulting in narrowing of the lumen and if left untreated can clog arteries and lead to cardiovascular disease. To prevent this, it can be done following a healthy lifestyle as endeavored by the Ministry of Health of the Republic of Indonesia. This community service aims to increase public knowledge and provide cholesterol check services in the prevention of cardiovascular disease that is carried out in Sukawati I District, Gianyar. The results showed that before counseling 5.4% of respondents answered 75% or more of the questions correctly. After being given an explanation accompanied by a leaflet, the respondent's knowledge became marked by an increase in the ability to answer that is 97.3% able to answer questions above 75%. Respodents with cholesterol levels above normal are most often found in respondents who have less knowledge that is 50%, the rest are found in respondents with sufficient and good knowledge. Percentage of respondents with normal cholesterol levels is most often found in respondents who have sufficient knowledge that is 74%. From these data it can be concluded that the total cholesterol level of respondents above normal is most often found in respondents with less knowledge. Thus, it is necessary to do continuous counseling to be able to change behavior towards supporting the prevention of cardiovascular disease.

PENGARUH PENGGUNAAN EKSTRAK BUNGA PACAR AIR (Impatiens balsamina L.) SEBAGAI ANTISEPTIK TERHADAP ANGKA KUMAN PADA TELAPAK TANGAN MAHASISWA POLTEKKES DENPASAR Dewa Ayu Pradnyani Putri; I Nyoman Gede Suyasa; Cok Dewi Widhya HS; Ida Bagus Oka Suyasa
Jurnal Skala Husada : The Journal of Health Vol 21, No 1 (2024): Jurnal Skala Husada (JSH)-The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jsh:tjoh.v21i1.3314

Pacar air flowers (Impatiens balsamina L.) hold potential as natural antibacterial agents, rich in compounds like flavonoids, alkaloids, steroidal saponins, tannins, quinones, and phenols. Pacar air flowers are utilized in traditional medicine for various medical conditions, including antibacterial properties. The purpose of this study is to determine the effect of using balsam flower extract (Impatiens balsamina L.) as a natural antiseptic in reducing total plate count on the hands of Poltekkes Denpasar students. Sterile hand swab samples were collected using a Pretest-Posttest Control Group Design to assess differences before and after the application of various concentrations. Statistical analysis was performed using One-way ANOVA. The study revealed differences in total plate count before and after the application of pacar air flower extract (Impatiens balsamina L.) on the hands of Poltekkes Denpasar students. The mean total plate count before treatment was 29,98 CFU/cm2, while after treatment, it was 4,70 CFU/cm2. Total plate count significantly decreased (p0.05) after applying the pacar air extract compared to before (Impatiens balsamina L.). There was a significant reduction in total plate count with the application of extract concentrations of 5%, 10%, and 15%

THE ETHANOL EXTRACT OF JUKUT PENDUL (KYLLINGA NEMORALIS) EXHIBITS INHIBITORY ACTIVITY AGAINST THE GROWTH OF STAPHYLOCOCCUS AUREUS Komang Deatriasa Pakerti Niluwih; I Gusti Agung Ayu Putu Swastini; Nur Habibah; Ida Bagus Oka Suyasa
Meditory : The Journal of Medical Laboratory Vol 11, No 2 (2023): Meditory Volume 11 No. 2 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i2.3060

Staphylococcus aureus is a pathogenic bacteria that is often found to have resistance to antibiotics. Jukut pendul (Kyllinga nemoralis) extract is known to have antibacterial compounds such as flavonoids, tannins, saponins, and steroids. The purpose of this study was to determine the inhibitory ability of jukut pendul ethanol extract against Staphylococcus aureus bacteria. This research used the Kirby-Bauer disc diffusion method with five treatments within five repetitions. Data analysis was carried out using One Way Anova variance analysis. The variation in extract concentration tested was 5, 10, 20, and 40% with 96% ethanol as the control group and Ciprofloxacin as the work control. The results showed that extract concentrations of 5, 10, 20, and 40% were able to inhibit Staphylococcus aureus bacteria with an average inhibitory zone of 9,36; 10,85; 11,64; and 12.15 mm, respectively. The inhibitory zone formed indicates that jukut pendul ethanol extract has moderate to strong inhibitory ability in inhibiting the growth of Staphylococcus aureus bacteria.

IRRITATION TEST OF LIQUID ANTIBACTERIAL SOAP COMBINING LEGUNDI AND GREEN BETEL LEAVE EXTRACTS ON WHITE RATS (Rattus norvegicus) Ida Bagus Oka Suyasa; Ni Nyoman Astika Dewi; Made Delia Budi Apriliapatni; Surya Natallia Bryan
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.4067

Background: Soap is a cleanser that is made with a chemical reaction. Soap preparations with active ingredients derived from nature are also widely circulated. Natural ingredients that have many active substances are legundi leaves and green betel leaves. Two antimicrobial agents from these ingredients working simultaneously on a homogeneous microbial population will have a synergistic effect.Objective: This study aims to determine the safety of using an antibacterial liquid soap formula with a combination of legundi and betel leaf extracts.Methods: This study is an experimental research that includes physicochemical testing of the soap formula, organoleptic evaluation, and irritation testing on white rats (Rattus norvegicus) using the Draize method with a posttest-only control group design. This research was analyzed descriptively. This study adheres to the ethical principle of beneficence, prioritizing the well-being and safety of all participants.Results: Organoleptic test, pH test, homogeneity test, and water content test meet Indonesian National Standard requirements, and there is no irritation in experimental animals.Conclusions: An antibacterial liquid soap formula with a combination of legundi and betel leaf extracts is safe.

PHYTOCHEMICAL COMPOUND LEVELS IN BAMA LEAF EXTRACT AND ITS INHIBITORY ACTIVITY AGAINST Salmonella Thypimurium ATCC 19430 Putu Ayu Suryaningsih; Ida Bagus Oka Suyasa; Komang Dewi Fridayanti
Meditory : The Journal of Medical Laboratory Vol 11, No 2 (2023): Meditory Volume 11 No. 2 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i2.2925

Diarrhea is one of the intestinal infection symptoms that can cause poor absorption to loss of nutrition if not treated immediately. Salmonella spp. is 1 of the 4 fundamental global causes of diarrhoeal diseases and antibiotics have been widely used to treat infections caused by this group of bacteria. WHO reported that the antibiotic resistance of Salmonella spp. has increased in past years. For those reasons, efforts have been made to discover new antimicrobial compounds from various sources, including the ethanol extracts of Bama leaves. Therefore, this study aimed to determine the phytochemical compounds (flavonoids, tannins, and phenols) in the ethanol extract of Bama leaves and their inhibitory effect against the bacteria Salmonella typhimurium which causes diarrhea. Design method used is RAL 5 x 4, 5 treatments were concentration of Bama leaf extract 6,25%, 12,5%, 25%, 50% and 75% and 4 replicates for each test. The phytochemical quantitative analysis showed bama leaf extract had 520,99 mgQE flavonoid / 100 gram, 1465,19 mgTAE tannin/100 g and 16531,78 phenol/100 g extract. All contrentration of bama leaf extract could not inhibit the growth of Salmonella thypimurium ATCC 19430.

DESCRIPTION OF URIC ACID LEVELS BUS DRIVER PT ARSA BUANA MANUNGGAL KARANGASEM Ni Putu Mentari Yulia; ida bagus oka suyasa; i gede sudarmanto; i nyoman gede suyasa
Meditory : The Journal of Medical Laboratory Vol 12, No 1 (2024): Meditory, Volume 12 No. 1 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i1.3209

Uric acid is the end result of breaking down a substance called purine. Purine substance is a natural substance which one of the chemical structure groups is the formation of DNA and RNA. Uric acid can be influenced by several factors such as age, long sitting while driving, consumption of alcoholic beverages, and consumption of foods high in purines. The aim of this study was to determine uric acid levels in PT.ABM drivers. The study sample consisted of 45 people selected by random sampling using the POCT method with the Autoclick Easy Touch GCU tool. The results showed normal uric acid levels in 32 people (71.1%) and high uric acid levels in 13 people (28.9%). High uric acid levels were more common at the age of 43-51 years in 5 people (45.5%), duration of sitting while driving was dominated by driving duration for ? 7 hours/day in 10 people (29.4%), while drivers 11 people (34.4%) consumed alcoholic beverages and 25 people (55.6%) consumed high-purine foods. The conclusion of this study is that high uric acid levels occur in respondents aged 43-51 years, driving for ? 7 hours/day, consuming alcoholic beverages and consuming foods high in purines.

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