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PENGARUH KONSORSIUM PBRM (PLANT BENEFICIAL RHIZOSPHERIC MICROORGANISM) DALAM NUE (NUTRIENT USE EFFICIENCY) PADA PERTUMBUHAN JAGUNG (Zea mays L.) Tyas Nyonita Punjungsari; Agung Setya Wibowo; Intan Fuji Arriani; Palupi Puspitorini
AGRORADIX : Jurnal Ilmu Pertanian Vol 3 No 1 (2019): Desember 2019
Publisher : Agriculture faculty, Darul ‘Ulum Islamic University (UNISDA) of Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52166/agroteknologi.v3i1.1710

Corn farm processing in Blitar Regency is very dependent on fertilization using chemicals. The use of chemicals themselves has a negative impact on the environment and health if used continuously. Therefore we need a solution to increase the absorption of nutrients from the soil by plants (nutrient use efficiency), namely the use of beneficial microorganisms, namely PBRM (Plant Beneficial Rhizospheric Microorganism). The purpose of this study was to determine the effect of a consortium of soil from peanut plants in increasing the growth of corn plants. This research was conducted in May 2019 until September 2019. This study used RAK and was carried out in several stages, the first being planting corn seeds with a combination of NPK and PBRM Consortium, with each of 4 dose levels, K0, K1, K2, K3 Observation indicators consisted of plant height, leaf area, wet weight of cob, dry weight of cob. DMRT test results on the variable Plant Height that the treatment of K2 (1.5 kg Consortium per plant) showed the best results with an average of 7 DAP (9.40) 14 dd (40.96) 28 DAP (102.36) 35 DAP (141, 31) and 42 DAP (172.21) while the age of 21 DAP in the K3 treatment (3 kg consortium) with the average (74.80) which showed the best results on corn plant height. Based on analysis of variance of leaf area observations, it was shown that the administration of PBRM consortium in NUE had a significant effect on the age of 21 DAP, 28 DAP, 35 DAP, and 42 DAP but did not significantly affect the age of 14 HST. In the variable wet weights of K2 treatment cob showed the highest average of 527.33 grams while in the variable dry weight of K2 treatment cob showed the highest average of 493.63 gr.

CHARACTERIZATION OF SULFATE REDUCING BACTERIA FROM BIOFILM SULFATE REDUCTION AND CU PRECIPITATION BIOREACTOR (BATCH CULTURE). Tyas Nyonita Punjungsari
JARES (Journal of Academic Research and Sciences) Vol 2 No 2 (2017): September 2017
Publisher : Universitas Islam Balitar

The biofilm is a microbial community structure formed on the zeolite surface in a sulfate reduction bioreactor and Cu deposition using a SRB consortium . The biofilm soluble microbial solvent is expected to have the capability in sulfate reduction and Cu deposition. Characterization of isolates is required for the optimization of pure culture . The aim of this study is to isolate and characterize the biofilm sulfate reducing bacteria in the sulfate reduction bioreactor and the precipitation of Cu in Batch Culture by a consortium of Sulfate Reducing Bacteria. The method used in this study cultivation was done by using postgate B medium, isolation was done by diluting biofilm on NaCl salt, bacteria grown on NB and postgate B media, characterization done by morphology and biochemistry. There were 3 isolates of B1 (Metylobacterium ), B3 ( Desulfucoccus ), and B2 ( Desulfobacter ). B3 ( Desulfococcus) has the best ability to reduce sulfate and Cu precipitation.Keywords : Sulfur Reducing Bacteria (SRB), Biofilm, Sulfate, Cu. Received: 26 August, 2017; Accepter: 10 September, 2017

MEDIAN LETHAL CONCENTRATION EKSTRAK DAUN MIMBA Azadirachta indica PADA ULAT GRAYAK (Spodoptera litura F.) SEBAGAI INDIKATOR BIOINSEKTISIDA Tyas Nyonita Punjungsari
Jurnal Ilmiah Hijau Cendekia Vol 7 No 2 (2022): Jurnal Ilmiah Hijau Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/hijau.v7i2.2007

ABSTRACT This experiment was conducted to determine the effect of mimba (Azadirachta indica) leaf extract as an alternative natural insecticide to S. litura in terms of the median lethal concentration (LC50). The research method used was a completely randomized design (CRD), with three replications, each replication was treated by adding 4 doses of mimba (Azadirachta indica) leaf extract (5%, 10%, 20% and 40% ppm). Data were collected at 24, 48, 72, 96, 120, 144, and 168 hours. Data analysis used probit analysis with SPSS 23, 95% confidence level. The results obtained from the study were that to kill S. litura larvae within 24 hours required a concentration of 91.670% neem leaf extract, while the lowest concentration required to kill S. litura was 3.425% within 168 hours.

PENGARUH KONSORSIUM PBRM (PLANT BENEFICIAL RHIZOSPHERIC MICROORGANISM) DALAM NUE (NUTRIENT USE EFFICIENCY) PADA PERTUMBUHAN JAGUNG (Zea mays L.) Tyas Nyonita Punjungsari; Agung Setya Wibowo; Intan Fuji Arriani; Palupi Puspitorini
AGRORADIX : Jurnal Ilmu Pertanian Vol 3 No 1 (2019): December 2019
Publisher : Agroteknologi Fakultas Pertanian Universitas Islam Darul 'Ulum (UNISDA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52166/agroteknologi.v3i1.1710

Corn farm processing in Blitar Regency is very dependent on fertilization using chemicals. The use of chemicals themselves has a negative impact on the environment and health if used continuously. Therefore we need a solution to increase the absorption of nutrients from the soil by plants (nutrient use efficiency), namely the use of beneficial microorganisms, namely PBRM (Plant Beneficial Rhizospheric Microorganism). The purpose of this study was to determine the effect of a consortium of soil from peanut plants in increasing the growth of corn plants. This research was conducted in May 2019 until September 2019. This study used RAK and was carried out in several stages, the first being planting corn seeds with a combination of NPK and PBRM Consortium, with each of 4 dose levels, K0, K1, K2, K3 Observation indicators consisted of plant height, leaf area, wet weight of cob, dry weight of cob. DMRT test results on the variable Plant Height that the treatment of K2 (1.5 kg Consortium per plant) showed the best results with an average of 7 DAP (9.40) 14 dd (40.96) 28 DAP (102.36) 35 DAP (141, 31) and 42 DAP (172.21) while the age of 21 DAP in the K3 treatment (3 kg consortium) with the average (74.80) which showed the best results on corn plant height. Based on analysis of variance of leaf area observations, it was shown that the administration of PBRM consortium in NUE had a significant effect on the age of 21 DAP, 28 DAP, 35 DAP, and 42 DAP but did not significantly affect the age of 14 HST. In the variable wet weights of K2 treatment cob showed the highest average of 527.33 grams while in the variable dry weight of K2 treatment cob showed the highest average of 493.63 gr.

SNP (Single Nucleotide Polymorphism) at Adiponectin Gene in Type 2 Diabetes Mellitus (T2DM) Patients TYAS NYONITA PUNJUNGSARI
El-Hayah Vol 9, No 2 (2023): EL-HAYAH (VOL 9, NO 2 Maret 2023)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v9i2.20643

SNPs haplotype blocks can be used as genetic markers to trace particular diseases (means indirectly tracks the causative genes). One of the SNPs that affect disease is SNP positions 45 and 276. SNPs 45 and 276 in the APMI or ADIPOQ or Adiponectin that affect insulin production on T2DM patients. The aim of this research was to identify the presence of SNP 45 in patients with type 2 diabetes mellitus. Method of this research is descriptive exploration using type 2 diabetes mellitus patients in Gledug Village, Blitar Regency as specimen donors. Identification using DNA extraction, DNA Amplification, sequencing and bioinformatics analysis. The results of this project seven DNA extracted, the seven sequences have the same Qv20+ value as the sequence base pair, alignment analysis using blast, when compared with the adipoQ gene sequence with accession number NG_021140.1, it was found that the KT, SR and BP sequences had a 100% similarity level. Identification of candidate SNPs in the absence of the adenine nucleotide was found in the SR, KT, and ST sequences

Macrozoobenthic biodiversity: an indicator of water quality in Ngrowo River, Tulungagung, East Java Punjungsari, Tyas Nyonita; Azizah, Luthfi Ainul
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 2 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i2.39800

.

SNP (Single Nucleotide Polymorphism) at Adiponectin Gene in Type 2 Diabetes Mellitus (T2DM) Patients PUNJUNGSARI, TYAS NYONITA
El-Hayah:Jurnal Biologi Vol 9, No 2 (2023): EL-HAYAH (VOL 9, NO 2 Maret 2023)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v9i2.20643

SNPs haplotype blocks can be used as genetic markers to trace particular diseases (means indirectly tracks the causative genes). One of the SNPs that affect disease is SNP positions 45 and 276. SNPs 45 and 276 in the APMI or ADIPOQ or Adiponectin that affect insulin production on T2DM patients. The aim of this research was to identify the presence of SNP 45 in patients with type 2 diabetes mellitus. Method of this research is descriptive exploration using type 2 diabetes mellitus patients in Gledug Village, Blitar Regency as specimen donors. Identification using DNA extraction, DNA Amplification, sequencing and bioinformatics analysis. The results of this project seven DNA extracted, the seven sequences have the same Qv20+ value as the sequence base pair, alignment analysis using blast, when compared with the adipoQ gene sequence with accession number NG_021140.1, it was found that the KT, SR and BP sequences had a 100% similarity level. Identification of candidate SNPs in the absence of the adenine nucleotide was found in the SR, KT, and ST sequences

Trypsin: A Member Of The Serine Protease Enzyme Isolated From Fusarium oxysporum and Fusarium sp. Punjungsari, Tyas Nyonita
El-Hayah:Jurnal Biologi Vol 10, No 1 (2024): EL-HAYAH (VOL 10, NO 1 September 2024)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v10i1.29531

Trypsin/Porcine enzyme is widely used in the vaccine manufacturing industry, but due to its non-halal source (intestine of Baby Sus scrofa), it needs an alternative enzyme that has the same characteristics but is sourced from halal things. Fusarium sp. and Fusarium oxysporum are thought to have the ability to produce protease enzymes similar to Trypsin from pigs, which is a natural part of their infection system to cultivated plants. This study was conducted to isolate and characterize enzymes isolated from Fusarium oxysporum and Fusarium sp. Enzyme isolation was carried out using ammonium sulfate precipitation technique. Enzyme characterization using uv-vis spectrophotometer method by making variations of pH, temperature and type of substrate (specific gravity of substrate). Novel Enzyme Trypsin from Fusarium oxysporum isolate has optimum pH 7, optimum temperature 30oC, Vmax 8.183 mM/s and Km 0.9091 mM. Novel Enzyme Trypsin from isolate Fusarium sp. has an optimum pH of 7.5; optimum temperature of 30oC, Vmax 6.911 mM/s and Km 0.6192 mM.

PENGARUH MOLASE TERHADAP AKTIVITAS KONSORSIUM BAKTERI PEREDUKSI SULFAT DALAM MEREDUKSI SULFAT (SO4-) Punjungsari, Tyas Nyonita
Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian Vol 11 No 2 (2017): November 2017
Publisher : Universitas Islam Balitar

The purpose of this research is to understanding the effect of organic matter on BPS activity in reducing sulfate. Research carried out in batch culture using erlenmeyer, and using a completely randomized design (CRD). The treatment given is organic matter amounted to 308 mg / L, 617 mg / L and 1.234 mg / L and control. Each treatment was repeated 3 times. The parameters observed in this study is SO4- concentration. Using Duncan New Multiple Range Test (DNMRT) at 5% level for data analysis. The results showed that the molecular concentration of 617 mg / L was able to decrease the sulfate concentration at the fastest, then consecutively the concentration of molasses 1.234 mg / L, and control. Based on the Anova test the significant value is less than 0.05. Thus it can be stated that there is an effect of addition molasses to decrease sulfate concentration by sulfate reducing bacteria consortium.

PENGARUH PENAMBAHAN ABU SEKAM PADI (RICE HUSK ASH) DENGAN PROPORSI YANG BERBEDA TERHADAP PERTUMBUHAN SELADAI AIR (Lactuca sativa L.) Kurniastuti, Tri; Puspitorini, Palupi; Punjungsari, Tyas Nyonita
Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian Vol 11 No 2 (2017): November 2017
Publisher : Universitas Islam Balitar

Lettuce vegetables (Lactuca sativa L.) much needed by the community to meet the nutritional needs, but lettuce production is still low because of low productivity. Increasing the quality and quantity of vegetables grown on polybags is by fertilizing and arranging the composition of the planting medium. Provision of fertilizer aims to increase the supply of nutrients needed plants to boost the production and quality of crops. Fertilizer is to provide or add nutrients that plants need. However, fertilizer will tend to cause high production value and less appropriate with vegetable planting at home, therefore more optimized setting the composition of planting media. Planting media should be able to provide nutrients needed by plants. Farmers use plant residual organic materials such as rice husk as and cockpit for vegetable cultivation. The rice husk is the outermost part of the grain. The rice husk ash usually contains N 0.32%, P 0.15%, K 0.31%, Ca 0.96%, Fe 180 ppm, Mn 80.4 ppm Zn 14.10 ppm and pH 6, 8 which is expected to meet the needs of plant nutrients. The study was prepared using a complete randomized design with one factor that is the addition of planting media in the form of rice husk ash with the proportion of top soil and rice husk ash as follows: M1 (3: 1), M2 (2: 2), and M3 (1: 3). The observed variables were stem height, crown dry weight, and root dry weight. Data analysis used F Test 5% and continued with Duncan Multiple Range Test (DMRT) 5%. The results obtained at plant height of M3 (1: 3) gave the best result where there was a plant height increase of 40 cm from the first day of planting until the third day, the addition of rice husk ash did not affect the addition of root dry weight and dry weight of crown.

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