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All Journal Agroteknika Journal of Applied Agricultural Science and Technology
Eka Susila
Politeknik Pertanian Negeri Payakumbuh
Agriculture, Biological Sciences & Forestry Energy Engineering Mechanical Engineering

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Identifikasi Molekuler Trichoderma spp. Indigenous dari Rizosfer Beberapa Varietas Padi Asal Kabupaten Lima Puluh Kota dan Kota Payakumbuh Nelson Elita; Eka Susila; Agustamar Agustamar; Rizki Rizki
Agroteknika Vol 5 No 1 (2022): Juni 2022
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/agroteknika.v5i1.114

Abstract

Peranan mikroba meningkatkan hasil padi metode SRI beragam dipengaruhi jenis, kombinasi mikroorganisme, daya adaptasi, dan teknik aplikasi. Hasil penelitian menunjukkan aplikasi mikroba indigenous mampu meningkatkan hasil tanaman padi metode SRI. Jenis mikroba tanah jamur Trichoderma spp. umumnya banyak ditemukan merupakan jamur tanah biasanya ditemukan pada rizosfer tanaman, termasuk rizosfer tanaman padi. Tujuan penelitian untuk mengetahui jenis Trichoderma spp. indigenous secara makroskopis dan molekuler asal rizosfer beberapa varietas padi di Kabupaten Lima Puluh Kota dan Kota Payakumbuh. Metode yang digunakan adalah eksplorasi, isolasi dan karakterisasi. Eksplorasi Trichoderma spp. indigenous diambil dari tanah rizosfer padi di Kabupaten Lima Puluh Kota dan Kota Payakumbuh. Isolasi dan karakterisasi jamur Trichoderma spp. indigenous dilakukan di laboratorium Mikrobiologi Politeknik Pertanian Negeri Payakumbuh dan teknik molekuler dengan Amplifikasi PCR di Balai Penelitian Tanaman Buah Tropika di Solok. Hasil penelitian diperoleh isolat T1-KK (Kuriak Kuning), T2-PW (Pandan Wangi), T3- J (Junjuang) ketiganya asal Kabupaten Lima Puluh Kota, T4- S (Sokan), T5-KM (Ketan Merah), T6-SB (Siliah Baganti) asal Kota Payakumbuh. Hasil amplifikasi PCR diperoleh jenis isolat Trichoderma spp dari masing-masing isolat tersebut adalah T1-KK jenis jamur Trichoderma asperellum, T2-PW jenis jamur Trichoderma harzianum dan Trichoderma asperellum, T3-J jenis jamur Trichoderma harzianum dan Trichoderma asperellum, T4-S jenis jamur Thichoderma asperellum, T5-KM jenis jamur Trichoderma harzianum dan Trichoderma asperellum, dan T6-SB jenis jamur Trichoderma harzianum dan Trichoderma asperellum.
Exploration And Morphology Identification of Spores Asbuscular Mycorrhizal Fungi from Horticultural Plantation Eka Susila; Sari Rukmana Okta Sagita Chan; Benny Satria Achmad; Fri Maulina
Journal of Applied Agricultural Science and Technology Vol. 6 No. 1 (2022): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.922 KB) | DOI: 10.55043/jaast.v6i1.31

Abstract

Differences in location and rhizosphere cause differences in species diversity and population of Arbuscular Mycorrhizal Fungi (AMF). In addition, not all AMF have the same morphological and physiological characteristics, therefore it is very important to know their identity. This study aims to determine the presence and number of spores as well as to determine the morphological characteristics of AMF originating from the rhizosphere of several horticultural crops in the agricultural land of Payakumbuh State Agricultural Polytechnic. The method used in this research is descriptive exploratory method by means of purposive sampling for soil sampling. While the stages of this research include: taking and collecting data in the field, determining the point of location for soil sampling, taking soil samples, analyzing soil properties in the laboratory, isolating AMF spores and identifying AMF spores morphologically. The conclusions of this study are 1) The population of AMF spores in horticultural land is high. The highest spore population was found in soil samples of the root area of shallot plants (556 spores per 10 g of soil), while the lowest number of spores was in soil samples of eggplant root areas (271 spores per 10 g of soil), 2) AMF exploration in several horticultural crops in the agricultural land of the Payakumbuh State Agricultural Polytechnic, based on morphological identification (shape, color and size), the AMF found consisted of three genera, namely Glomus sp, Gigaspora sp, and Scutelospora sp.
The Effect of Indigenous AMF Applications on The Morpho-Physiological Characteristics of Two Varieties of Shallots on Drought Stress Conditions Eka Susila; Fri Maulina; Aswaldi Anwar; Auzar Syarif; Agustian Agustian
Journal of Applied Agricultural Science and Technology Vol. 7 No. 2 (2023): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v7i2.80

Abstract

One of the limiting conditions for shallot plants to grow optimally is dry land conditions. Indigenous AMF application is one way to overcome this condition. This study aimed to determine the effect of indigenous AMF application on the morpho-physiological characters of two shallot varieties which are sensitive and tolerant to drought stress conditions. The study was carried out for 6 months on a wirehouse and laboratory scale. The Experiment used a completely randomized design with two factors. The first factor was varieties of shallots, i.e. Brebes (Sensitive) and Kuning (Tolerant) varieties of shallots. The second factor was the application of indigenous AMF which consisted of 5 levels i.e. Glomus sp1, Glomus sp2, Glomus sp3, a mixed those three isolates and control treatment (without application of AMF). The morpho-physiological observation parameters included header dry weight, root weight, and leaf proline content. From the observations, it can be concluded that under stressed conditions, the leaf proline content of the sensitive variety accumulated higher in the header than the tolerant variety, because the tolerant variety was better able to produce higher root and header weights when adapting than the sensitive. AMF inoculation did not show significant differences with the treatment without AMF inoculation on leaf proline. However, there was a tendency that inoculation of a mix of AMF isolates (Glomus sp1+Glomus sp2+ Glomus sp3) decrease the proline content in the leaves, both in sensitive and tolerant varieties so that plants are more resistant to drought stress.
Co-Authors Agustamar Agustamar Agustian Agustian Anwar, Aswaldi Auzar Syarif Benny Satria Achmad Elita, Nelson Fri Maulina Rizki Rizki Sari Rukmana Okta Sagita Chan