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Agustina Tangapo
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Mathematics Medicine & Pharmacology Physics

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Potensi Metabolik Komunitas Bakteri Endofit dan Rhizosfer Ubi Jalar (Ipomoea batatas L.) Berdasarkan Analisis Community-Level Physiological Profiles (CLPP) Tangapo, Agustina
Jurnal MIPA Vol 9, No 1 (2020)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jmuo.9.1.2020.27159

Abstract

Aktivitas metabolik dari komunitas bakteri endofit dan rizosfir diukur untuk membandingkan tingkat community-level physiological profiling (CLPP) menggunakan Biolog Ecoplates. Hasil penelitian kami menunjukkan kepadatan bakteri meningkat dengan urutan: endofit<rhizosfer. Keragaman mikroba dinilai oleh kekayaan spesies, jumlah spesies dan indeks kesamaan. Aktivitas total mikroba tertinggi diamati pada mikroba rhizosfer. CLPP menunjukkan bahwa bakteri bisa memanfaatkan semua kelompok sumber karbon sebagai berikut karbohidrat, asam amino, asam karboksilat, polimer, amina/amida, dan senyawa fenolik. Karbohidrat yang paling dimanfaatkan.  Total metabolic activity of endophytic and rhizosphere of bacterial community of sweet potato was measured to compare the community-level physiological profiles (CLPP) using by Biolog Ecoplates. Result of our study revealed that bacterial density increased in the following order: endophytes < rhizosphere. The microbial diversity assessed by species richness, the total number of species present and species evenness. The highest total microbial activity was observed for the rhizosphere. The CLPP revealed that the bacteria could differentially utilize all the groups of carbon sources as follows carbohydrate, amino acid, carboxilyc acid, polymer, amine/amide, and phenolic compound. Carbohydrate was most utilized.
Identifikasi Trichoderma sp. dari Rizosfer Tanaman Kanonang (Chordia dichotoma. G. Forst) dan Karakteristik Pemacu Pertumbuhan serta Daya Antagonismenya Terhadap Fusarium oxysporum Rawis, Billy David; Pahlevi, Dylan R.; Siahaan, Parluhutan; Singkoh, Marina; Tangapo, Agustina
JURNAL BIOS LOGOS Vol. 14 No. 3 (2024): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v14i3.60630

Abstract

The use of biological control agents to inhibit the growth of pathogens in this case Fusarium in agricultural plants is very important in order to reduce the impact of environmental damage. Trichoderma sp. is a fungus that can be obtained from the rhizosphere of plants that can increase soil fertility and inhibit pathogenic fungi that cause damage to agricultural plants. The use of local isolates of antagonistic fungi in this case from the rhizosphere of the Kanonang plant gave results where the discovery of Trichoderma species that are able to control plant pathogens in this case Fusarium oxysporum through antagonism mechanisms such as inhibition, antibiosis and microparasitism. To find out the species of Trichoderma sp, it was continued with a molecular test using the ITS marker gene and the results of the test confirmed that the Trichoderma species in the rhizosphere of the Kanonang plant was the Trichoderma asperellum type. This type of Trichoderma also after being tested for its characteristics in stimulating growth gave positive results for both phosphate solvent and auxin producer (IAA) tests
Co-Authors Marina F. O. Singkoh Pahlevi, Dylan R. Parluhutan Siahaan Rawis, Billy David