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All Journal JURNAL TEKNOLOGI LINGKUNGAN BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI Makara Journal of Science
Evi Triana
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI), jalan Raya Bogor km. 46, Cibinong, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary

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Journal : JURNAL TEKNOLOGI LINGKUNGAN

 1 
AKTIVITAS AKTINOMISETES DARI BANGKA-BELITUNG KOLEKSI BIDANG MIKROBIOLOGI, PUSLIT BIOLOGI- LIPI DALAM MEMPRODUKSI ENZIM KITINASE Soeka, yati Sudaryati; Triana, Evi; Setianingrum, Ninu
Jurnal Teknologi Lingkungan Vol. 11 No. 3 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.075 KB) | DOI: 10.29122/jtl.v11i3.1187

Abstract

The aim of the research was to know the capability of actinomycetes isolate from Bangka Belitung, which stored at Microbiology-LIPI Culture Collection, in producing chitinase enzyme. This isolate which could produce chitinolitic enzyme, signed by clear zone at medium contain 1% chitine. The chitinase activity of the isolate which incubated for 1-7 days in the room temperature was analyzed by spectrophotometer in λ 584 nm. The result of this experiment was highest chitinase activities with incubated for 3 days, were 1.66 . 10-2 U/ml. Maximum chitinase activities was found at 1% starch soluble substrate 2.83 . 10-2, pH 8.0 and at 50°C condition were 9.3 . 10-2 and 12.98 .10-2 U/ml respectively.Key words : chitinase, clear zone, spectrophotometer
Co-Authors Dewi, Ratih M Endang Sukara Hastuti, Ratih Dwi Joko Sulistyo Joko Sulistyo Kasim, Ernawati KATSUHIKO ANDO MISA OTOGURO Nandang Suharna, Nandang Napitupulu, Rostiati NR NITA ROSALINDA PRAYITNO Novik Nurhidayat PUSPITA LISDIYANTI Rahayu, Sri Hartin Setianingrum, Ninu SHANTI RATNAKOMALA Soeka, Yati Yantyati Widyastuti Yati Sudaryati Soeka YULIN LESTARI Yulinery, Titin Yulinery, Titin