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All Journal JURNAL TEKNOLOGI LINGKUNGAN BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI Makara Journal of Science
Evi Triana
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI), jalan Raya Bogor km. 46, Cibinong, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary

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Journal : BERITA BIOLOGI

 1 
PENGARUH ANGKAK HASIL FERMENTASI BERAS OLEH Monascus purpureus JMBa TERHADAP AKTIVITAS ANTIOKSIDAN DAN GLUTATHION PEROKSIDASE (GPx) SERTA HISTOPATOLOGI HATI TIKUS GALUR SPRAGUE DAWLEY [The effect of Angkak from rice fermented by Monascus purpureus JMBa on antioxidant and glutathion peroxidase (GPx) activity and liver histopatology of Sprague Dawley Rats] Kasim, Ernawati; Triana, Evi; Yulinery, Titin; Nurhidayat, Novik
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i2.487

Abstract

Free radicals released from metabolism processes in the body were reactive and toxic to cells and tissues because it cause protein, cell membrane and nucleic acid damages which lead to cancer. It could be overcame if antioxidant system in the body is in good function.Nevertheles antioxidant system could be destructed by variety of conditions. Modern lifestyle that frequently consumed of high saturated fat, additives, and low fiber content in foods were potential risk for cancer and hypercholesterolemia. To overcome that risks with specific drugs were high cost. Therefore this research was conducted to take advantages of natural sources which potential for antioxidant and antihypercholesterolemia activities that could be fast, easy and inexpensive processing. One of the natural sources that meet the criteria was angkak resulted from rice fermented by Monascus purpureus. The result revealed that angkak contained lovastatin showed antioxidant and antihypercholesterolemia activities and increased glutathion peroxidase activity of optimal dose 5 g/day. The histopathologi observation of rat’s liver showed that administered of angkak on rats feed high level of cholesterol inhibited accumulation of fat in rat’s liver.
AKTIVITAS ANTIBIOFILM BAKTERI Escherichia coli OLEH BAKTERIOFAG SECARA IN VITRO [Escherichia coli biofilm in vitro eradication by bacteriophage] Triana, Evi
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4021.357 KB) | DOI: 10.14203/beritabiologi.v17i1.3234

Abstract

Several Escherichia coli strains are pathogenic. Excessive and noncompliant use of antibiotics and disinfectants may cause bacteria to build resistance mechanisms. Forming biofilms cause eradicatation more difficult. An effective cleaning action required antibiofilm and antimicrobial agents that have different mechanisms with antibiotics and disinfectants. Bacteriophages are potential candidates because they meet these requirements. Bacteriophages produce specific polysaccharide lyase enzymes capable of degrading biofilm extracellular polymeric matrix. Study was aimed to determine concentrations of specific bacteriophage showing Escherichia coli antibiofilm activity was conducted. The results of this study showed that the most effective concentrations bacteriophage EC RTH 04 to prevent, inhibit, and degrade Escherichia coli EC 3 biofilms were 106, 102, dan 102 respectively.
PENERAPAN TEKNOLOGI FERMENTASI PADA BIOPROSES FERMENTASI MINYAK KELAPA (FERMIKEL) [Bioprocessing of Fermented Coconut Oil by Application of Fermentation Technology] Joko Sulistyo; Sulistyo, Joko; Soeka, Yati Sudaryati; Triana, Evi; Napitupulu, Rostiati NR
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.461 KB) | DOI: 10.14203/beritabiologi.v4i5.1246

Abstract

Methods of extracting oil from coconut endosperm by fermentatbn were studied. The factors which must be controlled to break the emulsion and liberate oil were investigated. It was found that grinding conditions exerted a profound effect upon the stability of the coconut milk emulsion. The optimum condition for rapid fermentathn of coconut milk was related to the condition during incubation period. The fermentation progressed best under mild conditions (28°C-40°Cj. The fermentation was successful in breaking the emulsion at a relatively broad of range and titrable acidity. Coconut cream and small volume of coconut water and "lontar" (palmyra palmj-sap were incubated separately with some strains of Bacillus species, which were preincubated in a coconut tomato-extract sugar (CTSj medium using a shaker, and grown as a starter under conditions that allowed for coconut oil production at pH 4,0-5,0 and 30 C°- 40 "C for 12-24 h. The organism destabilizes the emulsion, apparently by metabolizing sugars, resulting in the production of protein curd and high-quality oil. The palm sap and coconut water to the cream ratio of fermentation medium influenced the performance of oil produced and the bacteria grew well and produced oil in non sterile systems. The oil recovered was about 25 to 20% while average amount of oil in the coconut is approximately 25-35%, which means that only 83,33 to 66,67% oil was recovered. The oil contained little free fatty acid and very low concentration of cholesterol (0,0095 mg/ml), while the traditional coconut oil and commercially palm oil were 0,0111 mg/ml and 0,0132 mg/ml, respectively.
IDENTIFIKASI GEN SELENOMETIL TRANSFERASE (smt) PADA ISOLAT Geobacillus sp. 20K YANG RESISTEN TERHADAP SELENIUM Triana, Evi; Nurhidayat, Novik; Yulinery, Titin; Kasim, Ernawati; Dewi, Ratih M
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.562 KB) | DOI: 10.14203/beritabiologi.v10i3.747

Abstract

The trace element Selenium is toxic at high concentration.Most of organisms living in selenium rich environment are selenium resistant.One of the resistance mechanisms is methylation,in which selenium is methylated and transformed to non-toxic selenium compound.The methylation is catalyzed by seleno methyltransferase (SMT) coded by smt gene. The gene are expressed by selenium tolerant plants. However, there was no available report yet on such specific gene in the bacterial genome. This study was carried out to determine smt homologous gene on selected selenium accumulator bacteria, Geobacillus sp. 20k, The smt gene of was determined by amplifying target DNA and analyses its sequences through homology search (BLAST). The result showed that the DNA and its protein part of thermophilic enzyme involved selenium metabolisms.
Co-Authors Dewi, Ratih M Endang Sukara Hastuti, Ratih Dwi Joko Sulistyo Joko Sulistyo Kasim, Ernawati KATSUHIKO ANDO MISA OTOGURO Nandang Suharna, Nandang Napitupulu, Rostiati NR NITA ROSALINDA PRAYITNO Novik Nurhidayat PUSPITA LISDIYANTI Rahayu, Sri Hartin Setianingrum, Ninu SHANTI RATNAKOMALA Soeka, Yati Yantyati Widyastuti Yati Sudaryati Soeka YULIN LESTARI Yulinery, Titin Yulinery, Titin