Mustika Tuwo
Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Hasanuddin, Makassar 90245 Indonesia

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Mikropropagasi Talas Satoimo Colocasia esculenta (L.) Schott var. Antiquorum melalui Meristem Apikal Mustika Tuwo; Elis Tambaru; Baharuddin Patandjengi
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 1 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i1.13093

Abstract

Japanese taro (satoimo) is a functional food source belonging to the Araceae family. Satoimo seedling production is conventionally constrained by limited land and uncertain climate, so that production decreases. One of the efforts to increase the production of satoimo seedlings can be done through plant tissue culture (in vitro). Meristem culture is an in vitro culture technique that is capable of producing plants free of viruses, bacteria and fungi. MS medium is a plant basal medium still requires the addition of growth regulators for growth and development of explants. This study aims to obtain the optimum concentration of growth regulator BAP (6-benzylaminopurin) which is able to produce the best taro growth in the apical meristem culture of satoimo taro. Explant sterilization is carried out at the tip of the shoot that emerges from the tuber. Then washed with detergent under running tap water for ± 30 minutes, followed by sterilization in Laminar Air Flow (LAF). Shoot tip explants were sterilized with 5.25% sodium hypochlorite plus 3 drops of tween 20. Soaking time varied depending on the treatment and then planted on MS medium with various concentrations of BAP. The parameters observed were the percentage of contamination, survival rate of explants, percentage of explant mortality, number of shoots, number of leaves and shoot height. Observations were made for 8 weeks. The mean and standard deviation were calculated using Microsoft Office Excel computer software. The results showed that the BAP concentration of 0.5 ppm was the best concentration for shoot growth of shoots and leaves, while the BAP concentration of 1 ppm was the best concentration for shoot height in the satoimo taro plant.
Efektivitas Ekstrak Daun Eceng Gondok Eichornia crassipes Sebagai Bahan Antikanker pada Sel Tumor MCF-7 dengan Metode In Vitro dan In Silico Eva Johannes; Andi Ilham Latunra; Mustika Tuwo; Sry Sukmawaty
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 1 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i1.13095

Abstract

The use of water hyacinth Eichhornia crassipes bioactive compounds has been developed, including as an anti-cancer base material. This study aims to examine the potential of water hyacinth leaf extract on MCF-7 tumor cells with the MTT method and the affinity of the water hyacinth leaf compound using molecular docking methods. The results showed the effect of water hyacinth leaf extract on MCF-7 cancer cell death due to the active compounds stigmasterol and phytol, while the docking results showed the best binding affinity value on stigmasterol and its receptor 1QR1 which was -9.4.