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All Journal AQUATIC SCIENCE & MANAGEMENT e-Journal BUDIDAYA PERAIRAN JURNAL PESISIR DAN LAUT TROPIS JURNAL PERIKANAN DAN KELAUTAN TROPIS The Studies of Social Sciences
Reiny A Tumbol
Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Social Sciences

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Journal : e-Journal BUDIDAYA PERAIRAN

 1 
Evaluation of Baker’s Yeast (Saccharomyces cereviciae) In Enhancing Non Specific Immune Response and Growth of Nile Tilapia Manurung, Usy N; Manoppo, Henky; Tumbol, Reiny A
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.1.1.2013.720

Abstract

This research was conducted to evaluate the efficacy of baker’s yeast in enhancing nonspecific immune response and growth of Nile tilapia. After two weeks of acclimatization in fiber tank, juveniles were put into glass aquarium at the density of 15 fish/ aquarium. Fish were fed pellet supplemented with baker’s yeast as treatment for four weeks at 5% of body weight per day, twice daily (08.00 and 17.00). Five doses of treatment used in this research were A (0 g yeast/kg pellet), B (10 g yeast/kg pellet), C (20 g yeast/kg pellet), D (30 g yeast/kg pellet), E (40 g yeast/kg pellet), Immune parameters included total leucocyte count (TLC) and phagocytosis activity were measured at the end of experiment. Fish growth was weighing at the end of research period. Anova was used to evaluate the effect of baker’s yeast on immune parameters and growth while to evaluate the different effect between treatment, Duncan test was used. Research result showed that after four week of feeding, TLC of fish fed diet supplemented with baker’s yeast significantly different compared to control fish. The highest TLC of fish was achieved in treatment B followed by treatment C. It was also found that phagocytosis activity of fish increased significantly in fish fed treatment diet with the highest PA was observed in treatment C. PA of fish in treatment C was different significantly compared to a treatment A, D and E but between treatment C and B, no significant difference was observed. Application of baker’s yeast in diet also significantly increased growth of fish (p=0,00). Fish fed diet supplemented with 20 g baker’s yeast/kg pellet has the highest weight gain compared to other fish in other treatments. It was concluded that the used of baker’s yeast at 20-30 g/kg pellet for four weeks could enhance nonspecific immune response and growth of Nile tilapia. Keywords: Saccharomyces cereviciae, total leucocyte count (TLC), Phagocytosis Activity, growth, Oreochromis niloticus
Kelayakan lokasi budidaya ikan di Danau Tondano ditinjau dari parameter fisika kimia air Kamsuri, Agus I; Pangemanan, Penky N.L; Tumbol, Reiny A
e-Journal BUDIDAYA PERAIRAN Vol 1, No 3 (2013)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.1.3.2013.2732

Abstract

The purpose of this study was to determine the current condition of the water quality of Lake Tondano in terms of physical and chemical parameters in a fish farming locations on Lake Tondano waters. Determination of sampling points at each station is placed vertically at three predetermined points from the guard house toward the front of the net, the distance between one point to the next point was ± 10 m; whereas for the analysis of water quality parameters was done in Clinical Pathology of Fish Diseases Laboratory, Faculty of Fisheries and Marine Science, Sam Ratulangi Manado and Manado Industrial Research and Standards Laboratory. Determination points were done by purposive sampling which refers to the physiographic location wherever possible in order to represent or describe these waters. The research was carried out for 6 weeks and was done in 2 stages, morning and afternoon. For direct measurement (in situ) was performed once a week which included parameters DO, pH, temperature, and brightness, while the laboratory tests were conducted for 2 weeks which included parameters Nitrite, Nitrate, Iron, Chlorine, Manganese, Chloride, Sulfate, Aluminum, ammonia and Phosphate. The results show that the locations of the four parameters of chlorine (CL2) with range (0.1 to 0.28) and the parameters of Ammonia with the range (0.0125 to 0.15) over the limit indicated on water quality standards. The parameters of temperature, DO, pH, brightness, nitrate, nitrite, sulfate, manganese, chloride, aluminum, iron and phosphate were still in the water quality standard PP No.82 of 2001.
Diagnosa Penyakit Bakterial Pada Ikan Nila (Oreocrhomis niloticus) Yang Di Budi Daya Pada Jaring Tancap Di Danau Tondano Ashari, Chairanitansyah; Tumbol, Reiny A; Kolopita, Magdalena E.F
e-Journal BUDIDAYA PERAIRAN Vol 2, No 3 (2014)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.2.3.2014.5700

Abstract

This research aimed to diagnose bacterial diseases on nile tilapia cultured in stick net cage in Lake Tondano. Fish samples were taken from three locations of net cages in Lake Tondano namely Paleloan, Eris and Remboken villages. Three samples of fish were taken from each location/village. The samples taken showed clinical symptoms of bacterial infection such as hemorrhagic on the abdomen, the base of the fin, anal and chest, irregular swimming fish, hemorrhagic on the gills, fish refuse food, remain silent on the outskirts of net cage. Bacteria were isolated by using a sterile loop from head kidney (anterior kidney), which is located on the front, below the spine near the head. Bacterial cultures were performed on TSA media by making a zigzag scratches. The petri dish containing bacterial isolates were subsequently incubated in an incubator at a temperature of 35oC for 24 hours. The water quality parameter data measured consisted of temperature, Dissolved Oxygen, pH, ammonia, brightness. The results of the study showed that farmed tilapia in Lake Tondano has been infected with Aeromonas hydrophila that causes MAS (Motile Aeromonad Septicemia). The water quality, both chemical and physics around the net cages in the Lake Tondano was still in good condition and suitable for fish cultivation.   Keywords: Oreocrhomis niloticus, Bacterial disease, septicemia aeromonad motile, fixed net cage, Lake Tondano
Identifikasi Penyakit Bakterial Pada Benih Sidat (Anguilla marmorata) di Balai Budidaya Air Tawar Tatelu Kusen, Kevin Octavian; Tumbol, Reiny A; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 3, No 1 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.3.1.2015.6935

Abstract

This study aimed to identify the bacterial disease in cultured eel elvers (Anguilla marmorata). The samples used for this study were taken from the Tatelu Freshwater Aquaculture Center. The samples taken were elvers (juvenile life stage of A. marmorata)  which showed clinical symptoms of bacterial infection such as bleeding in the chest, abdomen, and the base of the fin, passive movement, weakness, and loss of balance of the body, loss of appetite, some wounds on back fin, chest, and tail, and the body was not slippery/shiny. Three elvers with the size of 4-6 cm, and weigh about 0.2 g were taken as samples. The organ chosen to be targeted for isolation was head kidney. Each sample was isolated twice as replicates and streaked on Triptic Soy Agar (TSA) medium. First replicates were identified in the Tatelu Health Lab of Freshwater Aquaculture, and the second replicates were identified in North Sulawesi Provincial Health Lab. Bacterial identification was done through a series of observations and morphology of bacterial colonies through gram stain, followed by biochemical tests through oxidase test, catalase test, Triple Sugar Iron Agar (TSIA) test, H2S production test, motility test, O/F test, and Simmons Citrate Agar (SCA) test. Water quality parameters that were measured included temperature, disolve oxygen, pH, and ammonia. Based on identification of bacteria in cultured elvers in Tatelu Freshwater Aquaculture Center, the elver samples had been infected with bacterial  Aeromonas hydrophila. Keywords: elver, identification, Aeromonas hydrophila, Tatelu Freshwater Aquaculture Center
Detection of the presence Aeromonas sp on nile tilapia cultured in floating net cage in lake Tondano Tantu, Wandalia; Tumbol, Reiny A; Longdong, Sammy N.J
e-Journal BUDIDAYA PERAIRAN Vol 1, No 3 (2013)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.1.3.2013.2738

Abstract

The purpose of this study was to detect the presence of bacteria Aeromonas sp in tilapia (Oreochromis niloticus). Nine fish with an average body weight of 150-200 g were used in this study as samples. Sampling was conducted at three different locations which were representative of fish culture sites located on Lake Tondano. The number of sample gathered from each location was and 3 fish which were taken randomly. Fish samples were taken by using a scoop, then the samples were taken alive by placing in an oxygen-filled plastic separately and taken direcly to lab Fish Disease Control and Environmental Center, Tateli, Department of Marine and Fisheries of North Sulawesi province, for examination the presence of bacteria. Isolation of bacteria were conducted by taking samples from gill and kidney. This study was carried out from April - July 2013. Identification of the presence of bacteria was done through a series of observations of colony morphology and gram staining of bacteria, followed by a series of biochemical tests: oxidase test , catalase test , test TSIA , H2S production test , indole test , motility test , citrate test, and test O/F. It could be concluded that 22.22 % of farmed nile tilapia in Lake Tondano were infected with the Aeromonas sp with the following percentage from each site: Paleloan village 7.40% , 7.40% Toulimembet village, and 7.40% Eris village. Keywords: Aeromonas sp, isolation, nile tilapia, floating net cage, Lake Tondano
Penambahan bakasang pada pakan benih sidat (Anguilla marmorata) untuk meningkatkan sistem imun non spesifik Pinoke, Steven A.J; Tumbol, Reiny A; Kolopita, Magdalena E.F
e-Journal BUDIDAYA PERAIRAN Vol 3, No 3 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.3.3.2015.10361

Abstract

This study was aimed to examine the effect of immunostimulant bakasang (fermented product from fish) and to determine the optimal dose of bakasang in increasing the non-specific immune response of eels. The eels were obtained from Institute of Freshwater Aquaculture (BPBAT) Tatelu. Bakasang as immunostimulant ingredient contained Lactic Acid Bacteria (LAB) with the bacteria concentration of 105 CFU/ml was prepared by diluting with clean water to get the doses desired. The feed was subsequently mixed with the commercial fish feed in powder form and mixed well with water to form paste. This research used Two-ways ANOVA with 4 different types of treatments A (0 ml/kg feed),   B (50 ml/kg feed), C (100 ml/kg feed), D (150 ml/kg feed) and 3 repetitions. The fish were feed with treatments diets for 3 weeks with a dose of 3% body weight per day with a frequency of twice a day (at 08:00 am) and the afternoon (16.00 pm). The parameters measured was Total Leukocyte Count (TLC) which was measured every week (t0, t1, t2, t3). The results indicated that the addition of bakasang on eels’feed gave a significant increased on TLC, and that the highest TLC was found in the dose of 100 ml/kg feed. This shows that the optimal dose of bakasang in increasing the TLC of eels  was 100 ml/kg feed after administration of 3 weeks. Keywords: Eels, Bakasang, Total Leucocytes Count
Co-Authors Agus I Kamsuri Chairanitansyah Ashari Hengky Manoppo Henky Manoppo Ida N Jamal Jetti Treslah Saselah Julius Sampekalo Kevin Octavian Kusen Longdong, Florence V Magdalena E.F Kolopita Penky N.L Pangemanan Remy E. P Mangindaan Rosa D Pangaribuan Sammy N.J Longdong Stenly Wullur Steven A.J Pinoke, Steven A.J Sultan, Makkulau Usy N Manurung Wandalia Tantu