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All Journal Sainstek Jambura Edu Biosfer Journal Jurnal Pendidikan, Sains dan Teknologi
Wirnangsi D Uno
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AKTIVITAS LACTOBACILLUS BULGARICUS PADA FERMENTASI SUSU JAGUNG (ZEA MAYS ) DENGAN PENAMBAHAN SUKROSA DAN LAKTOSA Machmud, Nur Afni; Retnowati, Yuliana; Uno, Wirnangsi D
Sainstek VOL 07, NO 02, 2013
Publisher : Sainstek

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.247 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui aktivitas bakteri Lactobacillus bulgaricus pada fermentasi susu jagung dengan penambahan sukrosa dan laktosa. Aktivitas bakteri ditentukan melalui pengukuran kadar asam laktat dengan data pendukung jumlah bakteri dan pH lingkungan. Kadar asam laktat dianalisis menggunakan Metode Mann Acid Test. Penelitian didasarkan pada metode eksperimen menggunakan rancangan acak blok dengan 3 perlakuan (susu jagung murni, susu jagung dengan penambahan sukrosa dan susu jagung dengan penambahana laktosa) dengan 9 ulangan. Hipotesis diuji dengan analisis statistik nonparametrik menggunakan kruskall wallis dan untuk menentukan perbedaan pada setiap perlakuan dilanjutkan dengan uji Mann-Whitney. Hasil analisis menunjukkan bahwa penambahan sukrosa dan laktosa tidak berbeda nyata dengan hasil kadar asam laktat 1,624% dan 1,953%. Pada perlakuan fermentasi susu jagung murni berbeda nyata dengan kedua perlakuan tersebut dengan hasil kadar asam laktat yaitu 1,062%.
POTENSI PENGHASILAN HORMON IAA OLEH MIKROBA ENDOFIT AKAR TANAMAN JAGUNG (Zea mays) Retnowati, Yuliana; Uno, Wirnangsi D; Putri, Siti Humairah Eka
Sainstek VOL 06, NO 06, 2012
Publisher : Jurnal Sainstek

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The aim of the study was to obtain an endofityc microorganisms that potential to produce IAA hormone in the root of corn and to analyze the ability to produce IAA by manipulating of the growth medium that used the liquid waste of tofu industry. The method based on descriptive methode that describe the ability of ed endofityc microorganism in IAA hormone producing at the liquid waste of tofu industry. The isolation of endofityc bacterial based on Radu and Kqeen method (2002), then characterization and ion was done. The ability producing IAA hormone test was based on colorimetry method used spectrophotometer on 530 nm of wavelength. The results showed that there were six isolates of endophytic bacteria isolated from the roots of Bisi and Motoro II maize varieties which shows the character of colony morphology and cell-specific forms. The results of measurements of the ability of the hormone IAA in vitro income showed an increase in levels of IAA until the seventh day of incubation. the test results of hormone production on the medium tofu industrial wastewater know precisely a decline compared to in vitro tests and the highest IAA levels achieved at 48 hours of incubation.
Pembuatan Cuka Ekstrak Jagung (Zea Mays) Dengan Menggunakan Acetobacter Aceti Dan Saccharomyces Cerevisiae Agtavia, Velma; Botutihe, Lisnawati; Thalib, Moh Randy Putra Preatama; Hasan, Ani M; Uno, Wirnangsi D
Jurnal Pendidikan, Sains Dan Teknologi Vol. 4 No. 4 (2025): Oktober-Desember
Publisher : CV. ITTC INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47233/jpst.v4i4.3735

Abstract

This study aims to produce natural vinegar from corn extract (Zea mays L.) through a two-stage fermentation process: alcoholic fermentation using Saccharomyces cerevisiae followed by acetic acid fermentation using Acetobacter aceti. The evaluation included physical observation, pH measurement, lactic acid bacteria (LAB) testing, organoleptic assessment, and determination of acetic acid concentration through titration. The results indicated notable physical changes during fermentation, with pH decreasing from approximately 7 to 6 over 7–14 days, accompanied by the emergence of a distinctive vinegar aroma that signified acetic acid formation. LAB activity was confirmed by colony growth on Nutrient Agar (NA) media, demonstrating their role in establishing an acidic environment favorable for the second fermentation stage. Overall, the findings show that corn extract has strong potential as a fermentation substrate for natural vinegar production using a biological process.
Cemaran Bakteri Escherichia coli dan Salmonella sp. pada Thunnus albacares di Pangkalan Pendaratan Ikan Kota Gorontalo Hadji, Sri Yulianti; Uno, Wirnangsi D; Baderan, Dewi Wahyuni K; Retnowati, Yuliana; Husain, Ilyas; Lamangantjo, Chairunnisah J
Jambura Edu Biosfer Journal Vol 7, No 1 (2025): Jambura Edu Biosfer Journal
Publisher : Fakultas MIPA Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34312/jebj.v7i1.25872

Abstract

Ikan tuna (Thunnus albacares) merupakan salah satu komoditas hasil laut yang banyak ditemukan di Pusat Pendaratan Ikan (PPI) Kota Gorontalo dan sering dikonsumsi masyarakat. Penanganan pasca tangkap yang kurang baik dapat memicu kontaminasi bakteri sehingga berdampak pada penurunan mutu ikan. Penelitian ini bertujuan untuk mendeteksi cemaran bakteri Escherichia coli dan Salmonella sp. pada ikan tuna jenis T. albacares di PPI Kota Gorontalo. Penelitian bersifat deskriptif dengan pengambilan sampel dari tiga titik berbeda, masing-masing satu sampel. Pengujian E. coli dilakukan dengan metode MPN tiga seri tabung, sedangkan Salmonella sp. diuji menggunakan metode kultur pada media selektif Salmonella Shigella Agar (SSA). Hasil penelitian menunjukkan bahwa seluruh sampel ikan tuna mengandung E. coli dengan nilai MPN berturut-turut 7,8 × 10³ APM/gr, 6,5 × 10³ APM/gr, dan 14,1 × 10³ APM/gr, sehingga tidak memenuhi syarat mutu SNI 2729:2013 yang mensyaratkan E. coli kurang dari 3 APM/gr untuk ikan segar. Sebaliknya, uji Salmonella sp. menunjukkan hasil negatif pada semua sampel, sehingga memenuhi baku mutu SNI 2729:2013 yaitu negatif per 25 gram (0/25 g).
Co-Authors Agtavia, Velma Botutihe, Lisnawati Chairunnisah J Lamangantjo Dewi Wahyuni K. Baderan Hadji, Sri Yulianti Hasan, Ani M Husain, Ilyas Nur Afni Machmud Siti Humairah Eka Putri Thalib, Moh Randy Putra Preatama Yuliana Retnowati