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All Journal Jurnal Manajemen dan Agribisnis BERITA BIOLOGI Jurnal Medik Veteriner YARSI Medical Journal Jurnal Kedokteran Hewan
MIRNAWATI SUDARWANTO
Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Dramaga, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Veterinary

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GEJALA KLINIS DAN PATOLOGI ANATOMI PASCA INFEKSI Campylobacter jejuni PADA AYAM BROILER [Clinical Symptoms and Pathology Anatomy due to Infection Campylobacter jejuni on Broilers] Andriani, Andriani; Sudarwanto, Mirnawati; Setiyaningsih, Surachmi; Kusumaningrum, Harsi Dewantari; Pisestyani, Herwin
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.54 KB) | DOI: 10.14203/beritabiologi.v11i3.498

Abstract

Campylobacter spp. is a bacteria foodborne zoonotic agent that can infect humans and animals, especially birds. This bacteria can cause important problems in the public health since the beginning at the farm level, the level of retail and on the table. Poultry meat is the main source of contamination because the digestive tract of poultry is the predilection of Campylobacter jejuni. During this infection C. jejuni in poultry did not show typical clinical symptoms, making detection quite difficult to diagnose this disease at the farm level. Incidence infection of Campylobacter sp. in developed countries had 5-90%. Currently poultry production and consumption are predicted to still increase due to a high demand for low price protein. This research is conducted to determine the clinical and pathologic anatomy due to an infection of C. jejuni on chicken digestive tract. A total of 105-day-old chickens are divided into 3 groups, each consisting of 35 individuals. Group A was the control group (not infected) group B (infected with C. jejuni ATCC 33291), and group C (infected with field isolate of C. jejuni/C1). Infection of the chicken is done 0.5 ml peroral suspension concentration of104cfu/ml. Anova Duncan is used to analyze parameters of body weight, increase weight gain, feed consumption, FCR values. Kurskall Walis method used to analyze the parameters non-parametric form of the score changes in the macroscopic and microscopic intestinal and liver. Infection of C. jejuni changes the pathology anatomy of intestine i.e. hyperemia, enteritis and enteritis catharalis haemorrhagic. A microscopic change of intestinal is oedema,haemorrhage and infiltration of inflammatory cell. Changes of pathology anatomy liver are colour smeary, congesti, pale, fragile and necrotic. Microscopic changes of liver are degeneration and focal hepatic necrosis. Group observation infected by C. jejuni caused chicken cannot reach maximum body weight and can be detrimental to farmers because of increase of the value of the feed conversion ratio (FCR).
BIOSINTESIS ANTIGEN PERMUKAAN HEPATITIS B “HBSAG100” PADA ESCHERICHIA COLI DALAM RANGKA PRODUKSI PROTEIN REKOMBINAN SEBAGAI MODEL IMUNOGEN UNTUK MENGHASILKAN ANTIBODI Riyadi, Slamet; RA Maheswari, Rarah; Sudarwanto, Mirnawati; RZ, Fransiska; Ali, Muhamad
Jurnal Kedokteran YARSI Vol 18, No 2 (2010): MEI - AGUSTUS 2010
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.94 KB) | DOI: 10.33476/jky.v18i2.188

Abstract

Biosintesis protein rekombinan melalui Escherichia coli memberikan alternatif untuk menghasilkan protein antigen yang bermanfaan bagi kepentingan kesehatan yang bebas dari protein manusia. Penelitian ini menggabungkan fragmen DNA dari antigen permukaan virus Hepatitis B dengan gen penyandi enzim gluthation-S-transferase (GST) di dalam plasmid p GEX-4T-2 yang di ekspresikan di dalam sel-sel Escherichia coli. Polypeptida dengan berat molekul sekitar 34,8 kDa telah diproduksi dan diidentifikasi sebagai protein gabungan GST-HB100. Protein gabungan tersebut kemudian dimurnikan menggunakan kolum GSTrap yang disambung dengan kolum HiTrap. Selanjutnya, protein hasil pemurnian tersebut diharapkan bisa digunakan sebagai bahan vaksin atau untuk menghasilkan antibodi.
Sanitation and Hygienic Practices of Ready-to-Drink Milk Seller Based on Total of Coliform and Staphylococcus aureus Pisestyani, Herwin; Ramadhani, Nada Nursaffana; Sudarwanto, Mirnawati; Lukman, Denny Widaya; Wicaksono, Ardilasunu
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.14-22

Abstract

Dairy products are innovative to improve the economic value of milk. Poor attention to the importance of good hygiene and milk handling of milk can lead to microbial contamination of milk, such as coliform and Staphylococcus aureus. This study aimed were to define the safety of ready-to-drink milk which sold around Dramaga and Cilibende IPB University campuses based on the total coliform and S. aureus and to determine the factors that affected it. Data were obtained by questionnaire from 12 sellers, furthermore milk samples were taken from 13 ready-to-drink milk's sellers with 3 replication The data were presented descriptively and analyzed using chi-square test to determine the relationship between the practice of the sellers with total contamination of coliform and S. aureus. The data results were compared with SNI 01-7388-2009 concerning the Maximum Limits of Microbial Contamination in Food. The average of coliform contamination in Dramaga and Cilibende 5,92í—103 ± 9,28í—103 CFU/ml and 7,60í—104 ± 1,25í—105 CFU/ml, respectively. The average of S. aureus contamination in Dramaga and Cilibende 3,84í—102 CFU/ml ± 5,36í—102 and 8,44í—104 ± 1,96í—105 CFU/ml, respectively. Furthermore, several practices of ready-to-drink milk sellers had a significant relationship with coliform contamination (p<0.05), which were the table cleanliness (p=0.020), distance to the crowd (p=0.001), and hand washing (p=0.001). The practice of ready-to-drink milk sellers had a significant relationship with S. aureus contamination (p<0.05) which were the table cleanliness (p=0.020), and distance of the crowd (p=0.020). The high contamination by coliform and S. aureus is caused by the poor sanitation and personal hygiene of the milk seller during the serving and processing of the milk.
An Evaluation of Draminski Detector as an Early Detection Tool for Subclinical Mastitis in Dairy Cattle in Pondok Ranggon Farm Pisestyani, Herwin; Permana, Indra; Basri, Chaerul; Lukman, Denny Widaya; Sudarwanto, Mirnawati
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.6-14

Abstract

Subclinical mastitis is one of the main problems in the development of dairy farming in Indonesia. Several screening test tools have been developed to support of control program for this problem. This study aimed to assess the suitability of the Draminski detector as a screening tool for subclinical mastitis in dairy cows. Quaternary milk samples from 27 dairy cows during normal lactation in the Pondok Ranggon area, East Jakarta were collected to be evaluated for the possibility of coming from quarters suffering from subclinical mastitis. Each milk sample was tested with 3 types of tests, i.e. the Breed method (golden standard) which was based on the number of somatic cells, the device Milkchecker which was based on electrical conductivity, and the Draminski detector which was based on electrical resistance. The results showed that the Draminski detector had a poor level of compatibility with the Breed method as the golden standard (Kappa value 0,013) and the Milkchecker tool (Kappa value 0,036). This test method was also considered to have poor test performance in diagnosing cases of subclinical mastitis in dairy cattle with a sensitivity value of 3,5%, specificity 100%, positive predictive value 100%, negative predictive value 18,8%, estimated prevalence 2,9%, and the true prevalence was 81,7%. In conclusion, the Draminski detector cannot be used to replace the Breed method and Milkchchecker as a screening test tool in cases of subclinical mastitis in dairy farms in Indonesia which were generally reared in the dairy farms.
Manpower Planning in The Application of Teat Dipping To Control Sub Clinical Mastitis in The Small Dairy Farms Anggraini, Raden Isma; Sudarnika, Etih; Pisestyani, Herwin; Wicaksono, Ardilasunu; Sudarwanto, Mirnawati
Jurnal Manajemen dan Agribisnis Vol. 17 No. 1 (2020): JMA Vol. 17 No. 1, March 2020
Publisher : School of Business, Bogor Agricultural University (SB-IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (681.042 KB) | DOI: 10.17358/jma.17.1.26

Abstract

The application of teat dipping to control subclinical mastitis especially in small dairy farms is important; however, the application needs additional activity that has an impact on labor usage. This paper aimed to describe the labor usage to apply teat dipping for subclinical mastitis control in the small dairy farms. The convenience sampling techniques applied to select 34 farmers in Bogor as the respondents. Workload analysis and labor productivity were used in examining the application of dot dipping in various small dairy farms in Bogor City to control subclinical mastitis. This study was based on two types of experiments in small dairy farms in Bogor, West Java, namely teat dipping and without teat dipping. The findings show that the application of teat dipping had an impact on three things, namely an increase in labor usage and its workload, an increase in labor productivity and a decrease in the number of subclinical mastitis prevalence. This study concluded that labor was an important factor to control subclinical mastitis in small dairy farms. An increase in labor productivity had an impact on reducing the costs of mastitis treatment and finally increase farmer’s income. Keywords: mastitis, labor, small dairy farms, teat dipping, workload analysis
Antimicrobial Activity and LC Determination of Ethanol Crude Extract of 50 Pliek U, an Achehnese Traditional Fermented Food Nurliana, Nurliana; Sudarwanto, Mirnawati; Sudirman, Lisdar I.; W. Sanjaya, Angelina
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i1.9795

Abstract

Antimicrobial activity of pliek u crude extracts was evaluated against five bacterial species (Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella enterica serovar Enteritidis and Pseudomonas aeruginosa,)and one fungal species (Candida albicans). Pliek u has been consumed as spices and hot sauce and poultry feed. These foods were collected from household industry at Reudep village in Aceh Besar, Province of Aceh. The ethanol extract of pliek u were obtained by standard method. The antimicrobial activity was detected using paper disc method. The concentration of ethanol crude extract of pliek u (EEP) was determined with the dilution method. The lethality initial test has been detected by using Artemia salina L. bioassay to determine the toxic concentration based on the LC value of EEP. The ethanol crude extract (EEP) was active against all microbial strains. EEP showed 50 antimicrobial activity at a minimum inhibitory concentration (MIC) and a minimum microbicidal concentration (MMC) at 2.5-10 mg/ml and 10-20 mg/ml, respectively. The lethality concentration EEP gave the LC value of 3.36 50 mg/ml. It was concluded that ethanol crude extract (EEP) shows significant antimicrobial activity and it is not toxic.
The Initial Antibacterial Activity Tests of Pliek U Oil and Pliek U: an Acehnese Traditional Food Nurliana, Nurliana; Sudarwanto, Mirnawati; I. Sudirman, Lisdar; Sanjaya, A. W.
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i2.9781

Abstract

This initial research was intended to detect antibacterial activity of pliek u oil and pliek u. Pliek u oil consist of minyeuk simplah (MS) and minyeuk brok (MB), Pliek u consist of two kinds of solid waste namely wet pliek u (Ap1) and dry pliek u (Ap2). Pliek u was methanol extracted at concentration 10% (w/v). Pliek u oil and methanol extract of pliek u were evaluated for their antibacterial activity, against Bacillus subtilis and four strains of Enterophatogenic Escherichia coli (EPEC) employing agar disc diffusion method. No antibacterial activity was shown by MS. The MB exhibited a little effect 1-2 mm against bacterial tests. The results demonstrated that the higher antibacterial activity was shown by dry pliek u compare with wet pliek u with the inhibition zones from 6.67-10.33 mm and 6.00-7.33 mm respectively.
AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KARI (Murraya koenigii) TERHADAP Staphylococcus aureus, Escherichia coli, dan Pseudomonas sp. R, Rastina; Sudarwanto, Mirnawati; Wientarsih, Ietje
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2842

Abstract

Penelitian ini bertujuan mengetahui aktivitas antibakteri, konsentrasi efektif, dan pengaruh peningkatan konsentrasi ekstrak etanol daun kari (Murraya koenigii) terhadap daya hambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Pseudomonas sp. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri menggunakan metode difusi agar. Parameter yang diukur adalah besarnya diameter daya hambat yang terbentuk di sekitar kertas cakram. Hasil uji aktivitas antibakteri dianalisis dengan metode one way anova dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak 12,5; 25; dan 50% telah memberikan aktivitas daya hambat pertumbuhan bakteri uji. Konsentrasi efektif yang dapat menghambat bakteri Staphylococcus aureus dan Escherichia coli pada konsentrasi 50%, sedangkan bakteri Pseudomonas sp. pada konsentrasi 12,5; 25; dan 50%. Peningkatan konsentrasi ekstrak daun kari menunjukkan semakin luas diameter zona hambat pertumbuhan bakterinya. Penghambatan yang terjadi pada bakteri Staphylococcusaureus, Escherichia coli, dan Pseudomonas sp. tersebut, membuktikan bahwa daun kari mengandung senyawa aktif yang bersifat antibakteri, seperti flavonoid, fenol, alkaloid, dan saponin.
DAYA TAHAN HIDUP Toxoplasma gondii DALAM SUSU KAMBING SETELAH PASTEURISASI SUHU TINGGI WAKTU SINGKAT Saridewi, Rismayani; Lukman, Denny Widaya; Sudarwanto, Mirnawati; Cahyaningsih, Umi
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2824

Abstract

Tujuan dari penelitian adalah menetapkan daya tahan hidup takizoit Toxoplasma gondii galur RH dalam susu kambing setelah dipasteurisasi pada suhu tinggi dalam waktu singkat. Dalam penelitian ini digunakan metode in vivo dan mencit diinfeksi dengan takizoit Toxoplasma gondii galur RH secara intraperitoneal dengan konsentrasi 2,76x106 takizoit/ekor. Mencit dibagi atas tiga kelompok perlakuan, yaitu susu pasteurisasi dan takizoit yang dipanaskan pada suhu 72 C selama 15 detik (P), susu pasteurisasi dan takizoit tanpa dipanaskan sebagai kontrol positif (KP), dan susu pasteurisasi tanpa takizoit sebagai kontrol negatif (KN). Hasil penelitian menunjukkan bahwa tidak ditemukan takizoit di dalam cairan peritoneal pada P dan KN. Takizoit Toxoplasma gondii galur RH ditemukan pada KP yang mempunyai jumlah konsentrasi hampir sama sebelum dan setelah infeksi.
Co-Authors Abdulwahed A. Hassan Agatha W. Sanjaya Andriani Andriani Anggraini, Raden Isma Ardilasunu Wicaksono Chaerul Basri Denny W. Lukman Denny Widaya Lukman Etih Sudarnika Ewald Usleber Fransiska RZ Harsi D. Kusumaningrum Heinrich Enbergs Herwin Pisestyani I W.T. Wibawan IETJE WIENTARSIH Indra Permana, Indra Lisdar Idwan Sudirman Muhamad Ali NURLIANA NURLIANA RA Maheswari, Rarah Ramadhani, Nada Nursaffana Rastina R, Rastina Rismayani Saridewi, Rismayani Rochman Naim Sanjaya, A. W. Setyo Widodo Slamet Riyadi Surachmi Setiyaningsih Umi Cahyaningsih W. Sanjaya, Angelina Widagdo S. Nugroho