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All Journal Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Farmasi BIOEDUPAT: Pattimura Journal of Biology and Learning Rumphius Pattimura Biological Journal
Syahran Wael
Program Pendidikan Biologi, Jurusan PMIPA FKIP Universitas Pattimura Ambon, Jl. Dr. Tamaela, Kampus Universitas Pattimura Ambon
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Journal : Rumphius Pattimura Biological Journal

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Measurement of Nitrate Reductase Activity in Vivo Using Sepctrophotometry Method Mose, Windi; Dorlandt, Mauritius; Riupassa, Pieter Agusthinus; Wael, Syahran
RUMPHIUS Vol 7 No 1 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i1p025-030

Abstract

Spectrophotometry is a quantitative measurement technique of a compound based on the measurement of absorbance or light transmission passing through the compound. The spectrophotometric method can be used to analyze the content of compounds. One of the enzymes whose activity can be measured in vivo is the nitrate reductase enzyme. The nitrate reductase enzyme is an enzyme that plays a role in the synthesis of ammonium and amino acids. The results of measuring the activity of the nitrite reductase enzyme with spectrophotometry showed that the highest NRA value was found in the leaves of red chili plants and the lowest was in the sugarcane leaf sample. NRA can be used as a selection parameter to predict the results of a plant, and has the potential to be applied to seed plants.
Utilization of Sea Cucumber (Holothuria scabra) for Nutritious and Healthy Food Consumption in the Maluku Community Tuaputty, Hasan; Leiwakabessy, Fredy; Wael, Syahran
RUMPHIUS Vol 7 No 2 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i2p119-127

Abstract

Types of sea cucumbers consumed by communities in the Maluku region are primarily Bohadschia bivittata, Holothuria scabra, and Pentamera calcigera, which are found in sandy substrates, hence referred to as "sandy sea cucumbers." Proximate analysis results show the highest protein content at 6.19% in Pentamera calcigera and the lowest at 5.24% in Holothuria scabra. The highest fat content is 0.71% in Bohadschia bivittata, and the lowest is 0.55% in Pentamera calcigera. The highest ash content is 3.29% in Bohadschia bivittata, and the lowest is 3.10% in Pentamera calcigera. The highest moisture content is 90.65% in Bohadschia bivittata, while Holothuria scabra has nutritional values between the other two species. A significant relationship exists between knowledge and skills in managing nutritious meal preparation, as indicated by an R² value of 0.961. This suggests that 96.1% of the community's skill in preparing nutritious and healthy meals from sandy sea cucumbers is influenced by their knowledge, with only 3.99% attributed to other factors. In other words, the higher the community's knowledge about sea cucumbers as a nutritious and healthy food source, the better their skills in preparing nutritious meals.
Analysis Antioxidant of Clove Leaves (Syzygium aromaticum) as a Source of Bioactive Compounds Wael, Syahran; Arini, Ine; Muskita, Marike; Rakuasa, Heinrich
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal (in process)
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p047-054

Abstract

This study aimed to evaluate the antioxidant activity of the chloroform extract of clove leaves (Syzygium aromaticum) as a source of bioactive compounds. Unlike most previous studies that predominantly focused on essential oils or polar extracts of clove, this research specifically investigates the antioxidant potential of the semi-polar chloroform extract, which remains relatively underexplored. Extraction was performed using chloroform to obtain semi-polar compounds with potential biological activity. Phytochemical screening revealed the presence of phenolic compounds, flavonoids, tannins, and terpenoids, which are known to function as natural antioxidants. Antioxidant activity was assessed using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay based on the ability of the sample to neutralize free radicals. The results demonstrated an increase in percentage inhibition with increasing extract concentration, indicating a positive linear relationship between concentration and antioxidant activity. Linear regression analysis yielded an IC₅₀ value of 69.40 ppm, classifying the extract as having strong antioxidant activity. In comparison, ascorbic acid exhibited an IC₅₀ value of 8.75 ppm, indicating very strong antioxidant activity. This difference is attributed to the nature of ascorbic acid as a pure compound, whereas the chloroform extract consists of a complex mixture of secondary metabolites. The novelty of this study lies in highlighting the significant antioxidant potential of the semi-polar chloroform fraction of clove leaves, thereby expanding the understanding of solvent-specific bioactive compound distribution and providing new insight into its possible application in functional food and pharmaceutical development. Overall, the findings suggest that the chloroform extract of clove leaves has promising potential as a natural source of bioactive compounds for further development.
Co-Authors ARINI, INE Audrey Makatita Awan, Ali Baharuddin, Alifia Audy Didik Wahyudi Dorlandt, Mauritius Fredy Leiwakabessy Jaber, Zaid Mohamad Letlora, Marsya Elsina Mamonsary, Christian Mimi Salmawati Mose, Windi Muskita, Marike Nimreskosu, Asna Collins Nurjani, Siti Nusaly, Windy Natalia Pattiasina, Eifan Boyke Pay, Chrismenda Pieter Agusthinus Riupassa PUDJI ASTUTI Rakuasa, Heinrich Rehena, Johanis Fritzgal Salessy, Sitti Senen, Yan Berlin Theopilus W Watuguly Tuanany, Nahriah Tuapattinaya, Prelly Marsel Jolanda Tuaputty, Hasan Wael, Ahmed Wael, Anourine Wurlianty, Benjamina