Articles
<![CDATA[Characterization of a Drought-Inducible Dehydrin Promoter from Sugarcane (Saccharum officinarum L.) in Tobacco (Nicotiana tabacum L.) ]]>
<![CDATA[Iskandar, Hayati Minarsih]]>;
<![CDATA[Suhandono, Sonny]]>;
<![CDATA[Pambudi, Jembar]]>;
<![CDATA[Kristianti, Tati]]>;
<![CDATA[Putranto, Riza Arief]]>;
<![CDATA[Mose, Windi]]>;
<![CDATA[Sustiprijatno, Sustiprijatno]]>
<![CDATA[ Journal of Tropical Crop Science Vol 7 No 01 (2020): Journal of Tropical Crop Science ]]>
Publisher : <![CDATA[Department of Agronomy and Horticulture, IPB University ]]>
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DOI: 10.29244/jtcs.7.01.28-36
<![CDATA[Dehydrin (DHN) is known to play an important role in plant response and adaptation to abiotic stresses (drought, high salinity, cold, heat, etc.). Previous research reported the increased expression of DHN in sugarcane stems exposed to drought stress for 15 days which may be controlled by its corresponding stress inducible promoter. The DHN promoter was succesfully isolated from sugarcane variety PSJT 941 (Pr-1DHNSo) and was cloned to pBI121 expression vector fused to a β-glucuronidase (GUS) reporter gene. The aim of this research was the functional testing of the Pr-1DHNSo promoter through transformation into tobacco plant treated with in vitro drought stress. Genetic transformation of Pr-1DHNSo construct was conducted by Agrobacterium tumefaciens. The transformed tobacco was then subjected to drought stress treatment using 40% PEG 6000 for five sequential incubations (0, 12, 24, 48 and 72 hours). The GUS assay reveal that the transformed tobacco treated with drought stress showed a blue color denoting GUS activity in leaf, stem and root tissues and this expression increased along with the length of the drought treatment. The analysis of gusA gene using real time-qPCR normalized to the L25 reference gene also showed that the expression increased in line with the length of time of drought stress. The results presented in this study indicated that the Pr-1DHNSo promoter from sugarcane was expressed and induced by drought stress treatment in tobacco.]]>
<![CDATA[Potential of Hotong Flour (Setaria italica (L.) Beauv) from Buru Island in Reducing Blood Cholesterol Levels in Wistar Rats with Diabetes Mellitus ]]>
<![CDATA[Mose, Windi]]>;
<![CDATA[Wael, Syahran]]>;
<![CDATA[Salmawati, Mimi]]>;
<![CDATA[Wahyudi, Didik]]>;
<![CDATA[Astuti, Pudji]]>
<![CDATA[ RUMPHIUS Vol 6 No 1 (2024): RUMPHIUS Pattimura Biological Journal ]]>
Publisher : <![CDATA[Universitas Pattimura ]]>
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DOI: 10.30598/rumphiusv6i1p001-005
<![CDATA[Hotong flour (Setaria italica) can be used as a functional food as therapy in the treatment of diabetes mellitus by reduce cholesterol levels in the blood. Hotong is a rice-like food crop that is usually consumed by the people of Buru Island. Hotong seeds have quite high nutritional content, namely containing 11.18% protein, 2.36% fat, 73.36% carbohydrates, 11.78% water, and 1.32% ash. The energy produced per 100 grams of hotong seeds is 359 calories. Hotong plants can be used as an alternative commodity in a carbohydrate-producing food diversification program. This study aims to determine the potential dose of hotong flour in treating diabetes mellitus by reducing cholesterol levels in the blood of rat. This research used 24 wistar rats animals which were divided into 6 groups which were given hotong flour for 21 days and the cholesterol levels were evaluated on the 7th, 14th, and 21st day. Determination of rat blood cholesterol levels using the enzymatic method. Hotong flour can reduce blood sugar levels at doses of 1 g/kg bw and 1.5 g/kg bw in rat that have been made diabetic by examination on days 7, 14 and 21 days.]]>
<![CDATA[OPTIMIZATION OF PROTOPLAST ISOLATION FROM DIFFERENT TYPES OF SAMPLES ]]>
<![CDATA[Mose, Windi]]>;
<![CDATA[Wael, Syahran]]>
<![CDATA[ RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal ]]>
Publisher : <![CDATA[Universitas Pattimura ]]>
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DOI: 10.30598/rumphiusv6i2p056-061
<![CDATA[Isolation of protoplasts in several plant species still requires optimization to produce protoplasts that are viable and can be used for further analysis. This research aims to optimize protoplast isolation protocols in several plants: Orchid Dendrobium macrocarpus, potato Solanum tuberosum, and microalgae Phaeodactylum tricornutum, Botryococcus braunii, and Spirulina sp. The results showed that in vitro explant selection had higher sample uniformity, and a combination of enzyme solutions could be used to increase the effectiveness of protoplast isolation on D. macrocarpus, S. tuberosum, and Spirulina sp. This study provides information about protoplast isolation techniques and testing their viability as an attempt for plant breeding through micropropagation.]]>
<![CDATA[IDENTIFICATION AND DENSITY OF MOSQUITO SPECIES THAT CAUSE FILARIASIS DISEASE IN TANIWEL WEST SERAM DISTRICT ]]>
<![CDATA[Senen, Yan Berlin]]>;
<![CDATA[Mose, Windi]]>;
<![CDATA[Rehena, Johanis Fritzgal]]>;
<![CDATA[Wael, Syahran]]>
<![CDATA[ RUMPHIUS Vol 5 No 1 (2023): RUMPHIUS Pattimura Biological Journal ]]>
Publisher : <![CDATA[Universitas Pattimura ]]>
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DOI: 10.30598/rumphiusv5i1p001-006
<![CDATA[The purpose of this study was to look at the types and density of mosquitoes in the Taniwel district. Research time is August 2021. This research is descriptive in nature. where only see the type and density using the existing formula. In this study, Mansonia and Anopheles mosquitoes were found. The results showed that mosquitoes found in East Taniwel sub-district, Sohuwe Village, Lumahlatal, and Maloang found 2 genera of mosquitoes namely Mansonia and Anopheles with mansonia density in Sohuwe village 3.29%, Lumahlatan 2.83% and Maloang 2.66. while the density of Anopheles in Sohuwe village was 3.62%, Lumahlatan 3.45% and Maloang 2.66%.]]>
<![CDATA[ANALYSIS OF THE HEAVY METAL CHROMIUM (Cr) IN MULLETS (Mugil dussumieri) SPECIES IN THE WAI RUHU GALALA RIVER AMBON CITY ]]>
<![CDATA[Wurlianty, Benjamina]]>;
<![CDATA[Mose, Windi]]>;
<![CDATA[Wael, Syahran]]>
<![CDATA[ RUMPHIUS Vol 5 No 1 (2023): RUMPHIUS Pattimura Biological Journal ]]>
Publisher : <![CDATA[Universitas Pattimura ]]>
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DOI: 10.30598/rumphiusv5i1p012-017
<![CDATA[The heavy metal chromium (Cr) is one of the heavy metals which is toxic and h armful if it accumulates in the body of organisms at high concentrations. Heavy metals can accumulate through the food chain, namely the higher the level of the food chain occupied by an organism, the accumulation of heavy metals in the body also increases. The aim of the research was to determine the liver contamination of mullet exposed to chromanum metal. The method used in this study was the purposive sampling method. Sediment and mullet at the Basic Kimia FKIP Laboratory while to measure the content of heavy metal Cr in sediment and mullet at the Karpan Health Laboratory, Ambon 11 Desember 2022 - 18 Januari 2023 using the AAS (Atomic Absorption Spectrophotometer). The Cr content in the sediment and mullet obtained at the estuary of the Wai Ruhu Galala river, Ambon City is still below a predetermined threshold. The research results obtained from the content of heavy metal cr in the sediment which is equal to 9,2888 Ppb while in mullet it is equal to 5,8185 Ppb. The presence of heavy metal cr is caused by contaminants which may originate from community activities around this location in the form of housedold waste coated with cr metal discharged directly into the water body such as household appliaces coated with cr metal and cans paint.]]>
<![CDATA[PEMANFAATAN BUAH MANGROVE (Sonneratia caseolaris) MENJADI SABUN CAIR ANTISEPTIK DI DESA ALLANG ASAUDE ]]>
<![CDATA[Patty, Kristi Lenci]]>;
<![CDATA[Nusaly, Windy Natalia]]>;
<![CDATA[Huwae, Laury Marcia Chara]]>;
<![CDATA[Mose, Windi]]>;
<![CDATA[Baszary, Chomsa Dintasari Umi]]>
<![CDATA[ Balobe: Jurnal Pengabdian Masyarakat Vol 3 No 2 (2024): Balobe: Jurnal Pengabdian Masyarakat ]]>
Publisher : <![CDATA[Pattimura University ]]>
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DOI: 10.30598/balobe.3.2.108-112
<![CDATA[Alang Asaode Village is one of the villages in West Seram Regency that has a large mangrove forest. Sonneratia caseolaris is one type of mangrove that has a fruit called pedada fruit. Pedada fruit has nutritional content that can be utilized into food products such as jam and syrup because of its high vitamin C content and can also be processed into antiseptic liquid soap. Community service activities in Alang Asaode village in the form of socialization of pedada fruit processing using the fermentation process into economically valuable products such as liquid soap to increase family income.]]>
<![CDATA[POTENTIAL OF CLOVE EXTRACT (Syzygium aromaticum) ON SPERMATOZOA RATS (Sprague dawley) EXPOSED TO ARAK AMBON (SOPI) ]]>
<![CDATA[Wael, Syahran]]>;
<![CDATA[Leiwakabessy, Fredy]]>;
<![CDATA[Mose, Windi]]>;
<![CDATA[Watuguly, Theopilus Wilhelmus]]>
<![CDATA[ BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 9 No 2 (2023): Biopendix: Jurnal Biologi, Pendidikan & Terapan ]]>
Publisher : <![CDATA[Program Studi Pendidikan Biologi FKIP Unpatti ]]>
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DOI: 10.30598/biopendixvol9issue2page211-221
<![CDATA[Introduction. Sopi is traditional drink from Maluku province which is often consumed in activities related to traditional events. Sopi drink comes from fermented Arenga pinnata which has undergone distillation. Clove leaf extract is a compound that has the effect of preventing cellular damage. Objective. To prove that administration of clove leaf extract can improve the spermatozoa of rats exposed to alcoholic sopi. Material and method. Experimental research with a post test only control group design. Spraguey dawley rats consisted of 24 rats and were divided into 4 groups consisting of a control group and a treatment group. The first treatment was given sopi as much as 4 ml/day, the second treatment was given sopi 4 ml/day for 16 days then continued with the administration of clove leaf extract at a dose of 1 ml/day for 17 days, the third treatment was given sopi 4 ml/day for 16 days then continued with provision of distilled water for 17 days. The different test for spermatozoa motility used the Kruskal-Wallis followed by the Mann Whitney test, while the number of spermatozoa used the One Way Anova test. Results. The average value of motility in the sopi treatment group (1,288) was lower than the control (1,637) and the sopi+extract treatment (1,670) was higher than the sopi+aquades (1,555). The results of the Kruskal-Wallis test for spermatozoa motility (p=0.011) showed a significant difference (p<0.05). Meanwhile, the average value of spermatozoa in the sopi treatment group (2.029) was lower than the control (2.228) and the sopi+extract (2.272) was higher than the sopi+aquades (2.145). Conclusion. The administration of the traditional drink arak sopi lowered the motility and number of spermatozoa, while the administration of clove leaf extract increased the motility and number of spermatozoa.]]>
<![CDATA[Measurement of Nitrate Reductase Activity in Vivo Using Sepctrophotometry Method ]]>
<![CDATA[Mose, Windi]]>;
<![CDATA[Dorlandt, Mauritius]]>;
<![CDATA[Riupassa, Pieter Agusthinus]]>;
<![CDATA[Wael, Syahran]]>
<![CDATA[ RUMPHIUS Vol 7 No 1 (2025): RUMPHIUS Pattimura Biological Journal ]]>
Publisher : <![CDATA[Universitas Pattimura ]]>
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DOI: 10.30598/rumphiusv7i1p025-030
<![CDATA[Spectrophotometry is a quantitative measurement technique of a compound based on the measurement of absorbance or light transmission passing through the compound. The spectrophotometric method can be used to analyze the content of compounds. One of the enzymes whose activity can be measured in vivo is the nitrate reductase enzyme. The nitrate reductase enzyme is an enzyme that plays a role in the synthesis of ammonium and amino acids. The results of measuring the activity of the nitrite reductase enzyme with spectrophotometry showed that the highest NRA value was found in the leaves of red chili plants and the lowest was in the sugarcane leaf sample. NRA can be used as a selection parameter to predict the results of a plant, and has the potential to be applied to seed plants.]]>
<![CDATA[Edukasi Peluang Bisnis Kultur Jaringan Anggrek Skala Rumah Tangga untuk Guru dan Siswa SMA Negeri Siwalima Ambon ]]>
<![CDATA[Mose, Windi]]>;
<![CDATA[Huwae, Laury M. Ch.]]>;
<![CDATA[Apituley, Edwin T.]]>;
<![CDATA[Salmawati, Mimi]]>
<![CDATA[ PENGAMATAN: Jurnal Pengabdian Masyarakat untuk Ilmu MIPA dan Terapannya Vol 2 No 1 (2024): PENGAMATAN: Jurnal Pengabdian Masyarakat untuk Ilmu MIPA dan Terapannya ]]>
Publisher : <![CDATA[Jurusan Matematika FMIPA Universitas Pattimura ]]>
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DOI: 10.30598/pengamatanv2i1p10-16
<![CDATA[Potensi anggrek untuk dijadikan komoditi dengan nilai ekonomi tinggi sangat menjanjikan, hal ini dikarenakan anggrek merupakan bunga potong yang tahan lama dan jika dibandingkan dengan tanaman lainnya memiliki keindahan bunga yang unik dan eksotik. Sifat bunga inilah yang menyebabkan anggrek banyak disenangi dan ditanam baik oleh para pengusaha tanaman hias maupun penggemar anggrek. Kultur jaringan merupakan salah satu cara perbanyakan tanaman dengan cara mengisolasi bagian tanaman seperti daun, batang, akar, dan biji serta menumbuhkan bagian-bagian tersebut dalam media buatan aseptik yang kaya nutrisi dan zat pengatur tumbuh, sehingga bagian tanaman tersebut dalam beregenerasi menjadi tanaman baru. Sebenarnya kultur jaringan dapat dilakukan dengan investasi yang relatif murah dengan memodifikasi peralatan, bahan, dan ruangan kultur jaringan. Kegiatan ini bertujuan untuk memberikan edukasi bagi guru dan siswa SMA Negeri Siwalima Ambon tentang teknik kultur jaringan sederhana serta informasi modifikasi peralatan dan bahan kultur jaringan sehingga dapat dilakukan pada skala rumahan.]]>
<![CDATA[PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF ETHANOL EXTRACT OF GALOBA DURIAN FRUIT PEEL (Ammomum spp.) ]]>
<![CDATA[Salamena, Fuadiska]]>;
<![CDATA[Manurung, Brian Saputra]]>;
<![CDATA[Mose, Windi]]>;
<![CDATA[Patty, Kristi Lenci]]>;
<![CDATA[Nusaly, Windy Natalia]]>;
<![CDATA[Putri Usman, Dwi Anggara]]>
<![CDATA[ Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025 ]]>
Publisher : <![CDATA[Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar ]]>
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DOI: 10.33992/meditory.v13i1.4021
<![CDATA[Background: Amomum spp. (red variant), An endemic plant widely found in Maluku, including Hatu Village, Ambon, is valued for its medicinal and economic roles.Objective: This study aimed to evaluate the phytochemical content and antioxidant activity of Ammomum peel extract using the DPPH assay.Methods: The fruit peel of Ammomum spp. (red variant) was extracted by maceration using ethanol as solvent and concentrated by evaporation at room temperature. The antioxidant activity was quantitatively and qualitatively assayed using the DPPH assay method and by observing the color change in the extract. Percent inhibition data were analyzed using linear regression.Results: The analytical findings confirmed the presence of flavonoids, saponins, tannins, steroids, and phenolics as secondary metabolite content in the galoba fruit peel extract. The free radical scavenging activity exhibited a strong value of 59.04 µg/mL. Therefore, this study suggests using the red variant of Ammomum spp. fruit peel extract in the treatment of free radical-induced disorders.Conclusions: Our study revealed that the peel extract of red Galoba durian contained secondary metabolites and strong antioxidant activity.]]>
