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All Journal Current Biochemistry Jurnal Pusat Inovasi Masyarakat Jurnal Manajemen, Akuntansi, Ekonomi Sahid Business Journal: Jurnal Penelitian Manajemen Bisnis Syariah: Program Studi Manajemen Bisnis Syariah Buitenzorg: Journal of Tropical Science
Syaefudin
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Social Sciences

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Journal : Current Biochemistry

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The Inhibitory Activity and Kinetics of α-Glucosidase by Toona sinensis Stem Bark Extracts Melati Devina Gustini Wirastuti; Syamsul Falah; Syaefudin
Current Biochemistry Vol. 9 No. 1 (2022)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.9.1.2

Abstract

Research on the potential of the Surian tree (Toona sinensis) for medicine has been carried out, especially for the leaves. However, the bark section has not been done much. This study aimed to determine the inhibitory activity of -glucosidase and IC50 extract as well as the fraction of surian bark as antidiabetic in vitro. The reflux method with water at 90oC and maceration using 70% ethanol at room temperature was carried out to obtain aqueous and ethanol extracts. Furthermore, the ethanol extract was fractionated successively using n-hexane, diethyl ether, and ethyl acetate. Phytochemical screening test showed that the aqueous extract contained flavonoids, saponins, and tannins. Meanwhile, the 70% ethanol extract and its fraction showed terpenoid content, in addition to flavonoid compounds, saponins, and tannins. The best -glucosidase inhibition was shown by 70% ethanol extract, which was 70.45 ppm followed by ethyl acetate fraction, n-hexane fraction, diethyl ether fraction, and water extract of surian bark with values of 81.96 g/mL, 98.76 g/mL, respectively. , 140.25 g/mL, and 242.85 g/mL. Kinetics of -glucosidase inhibition showed that 70% ethanol extract of surian bark inhibited through a non-competitive inhibition mechanism, characterized by a decrease in Vmax from 8.29x10-3 mM/min to 1.65x10-3 mM/min and a decrease in Km from 0.17 mM to 0.03 mM.
Peredaman Radikal Bebas DPPH dan Aktivitas Inhibisi α-Glukosidase oleh Ekstrak Air Jahe Merah Safithri, Mega; Syaefudin; Putri, Azka Adzkya Emalia
Current Biochemistry Vol. 10 No. 2 (2023)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.10.2.5

Abstract

Red ginger (Zingiber officinale Roxb. var. Rubrum) contains phenolic compounds that have potential to reduce blood glucose levels (antihyperglycemic) in patients with diabetes mellitus. This study aimed to determine the inhibitory activity toward α-glucosidase, antioxidant activity in reducing DPPH free radicals, and total phenolic content of aqueous extract of red ginger. The red ginger was extracted using water solvent at 100 oC for 15 minutes. Analysis of antioxidant activity, inhibitory activity of α-glucosidase, and total phenolic content using the spectrofotometry. Antioxidant activity showed that the extracts from the first, second and third replicates were not significantly different (p<0.05). α-glucosidase inhibition activity showed that the 1st replicate extract had the highest value of 97.729% and was significantly different (p<0.05) from the 2nd and 3rd replicate extracts and acarbose. The total phenolic content showed that the 2nd replicate extract had the highest value, namely 27,624 mg GAE/g extract and was significantly different (p<0.05) from the 1st and 3rd replicate extracts. The 1st, 2nd, and 3rd replicates of red ginger aqueous extract has the same antioxidant activity and equivalent glucosidase inhibitory activity as acarbose.
In Vitro Analysis of Gradual Water Extract of Red Betel Leaf (Piper crocatum) as Free Radical Scavenging and Inhibitor of α-Glucosidase Safithri, Mega; Fibri Yuniasih, Tiara; Syaefudin
Current Biochemistry Vol. 10 No. 1 (2023)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.10.1.5

Abstract

Red betel (Piper crocatum) has antihyperglycemic activity for diabetic treatment. The purpose of this study was to investigate the activity of DPPH free radical scavenging, the α-glucosidase inhibitory activity, and total phenolics of the aqueous extract of P. crocatum (1st extract, 1st extract residue, and 2nd extract residue). The infundation method was used with spectrophotometric test techniques. The red betel leaves extract in all extracts and extract residues had a small of α-glucosidase inhibition activity ranging from 0.76% - 1.43%. Meanwhile, the antioxidant activity of DPPH and total phenolic levels showed significantly different results (p<0.05) in the 1st extract, 1st extract residue, and 2nd extract residue. The highest antioxidant capacity and total phenolic content were found in the first extract, it were 59.64 ± 0.18 mg AAE/g extract and 55.50 ± 0.14 mg GAE/g, respectively. Gradual extraction reduced DPPH free radical scavenging activity, α-glucosidase inhibition activity and phenolic total content.
Co-Authors DIMAS ANDRIANTO Fadilah, Hafid Fibri Yuniasih, Tiara Gathan Ferbiansyah Kusworo Hemas Aulia Fazzahra Kinaya Aliqa Wijaya Kusnaedi, Anggi M. Iqbal Markus Amartya Sam Hutahaean Maulana Arham Mega Safithri Melati Devina Gustini Wirastuti Misla, Misla Nurhasanah Muhammad Faris Afif Muhammad Ikhwan Ramadhan Nurul Rahma Khaerunisa Putri, Azka Adzkya Emalia Resta Maryamzahra Rinol Sumantri Rolis Syamsul Falah Thantawi, Tubagus Rifqy