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Journal : HAYATI Journal of Biosciences

The Presence of Tobacco Mosaic Virus in the Compost Extract of Cigar Tobacco Debris WIWIEK SRI WAHYUNI; MUHAMMAD HANAPI; IGNASIUS HARTANA
HAYATI Journal of Biosciences Vol. 15 No. 3 (2008): September 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.751 KB) | DOI: 10.4308/hjb.15.3.118

Abstract

Tobacco mosaic virus (TMV) is resistance to high temperature and able to survive over 10 years on dried leaves, and plant debris is considered as source of inoculums of TMV in the field. In order to inactivate TMV, TMV-infected cigar tobacco debris was composted at starting temperature of 50 ºC for two to three days; however, TMV was still infective in the extract compost. If a half leaf cigar tobacco 'H877' was inoculated with compost extract, the symptoms appeared as a necrotic local lesion (NLL) and did not develop systemic lesions. The dilution end point of TMV in extract compost was 10-3. The number of lesion was higher in the glasshouse with average daylight temperature of 32 ºC than in the field with average daylight temperature of 29-30 ºC. The number NLL was lower and NLL size seemed to be smaller on the first and second inoculated leaves with extract than that of on the first and second inoculated leaves with TMV inoculums. There was a delay of time about 58-106 hours after inoculation of NLL from extract compost inoculums to appear than those of from TMV inoculums. These could be happened because of mineral nutrients of compost and also the temperature of maintaining tobacco plant which inhibited the infections, and of a thermal composting process which destroyed some TMV particles, particularly degraded it’s coat protein. Key words: TMV, extract water compost, cigar tobacco debris
Synergism of Lumbricus rubellus and Pseudomonas putida Pf-20 in Inducing Resistance to Cucumber Mosaic Virus WIWIEK SRI WAHYUNI; HARDIAN SUSILO ADDY; BUDI ARMAN; TRI CANDRA SETYOWATI
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (73.624 KB) | DOI: 10.4308/hjb.13.3.95

Abstract

Both Lumbricus rubellus and Pseudomonas putida decompose soil organic matters. The population of P. putida Pf-20 increased if L. rubellus was introduced to the cucumber growth medium. The process of organic decomposition was much better if the medium was introduced with both L. rubellus and P. putida Pf-20, compared to the medium contained only either one of those organisms. The activity of L. rubellus may serve to provide nutrients for both the cucumber and P. putida. The role of P. putida to reduce disease severity was increased if L. rubellus was introduced to the growth medium. The synergism of these two organisms, reduced either the level of disease severity to CMV-48 and C/N ratio of medium, but increased the content of available phosphor and potassium. Key words: Lumbricus rubellus, Pseudomonas putida Pf-20, disease severity of CMV
Compost Extracts of Vegetable Wastes as Biopesticide to Control Cucumber Mosaic Virus WIWIEK SRI WAHYUNI; ARIE MUDJIHARJATI; NIKEN SULISTYANINGSIH
HAYATI Journal of Biosciences Vol. 17 No. 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.918 KB) | DOI: 10.4308/hjb.17.2.95

Abstract

In semiaerobic conditions, different composting processes of vegetable wastes have different characteristics. When compost extracts amended with the effective microorganism-4 (EM4, +E) and Pseudomonas aeruginosa Ch1 (+B) stored for 40 days, the bacteria population and P-content increased. Tobacco plants treated with compost extracts amended with +E+B and [+E+B] directly to organic materials and inoculated with Cucumber mosaic virus (CMV) both sprayed or watered applications reduced the disease severity. This is due to the higher bacteria population in the root and rhizosphere, particularly the activities of P. aeruginosa Ch1 as plant growth promoting rhizobacteria (PGPR) rather than the activities of bacteria from EM4. The role of P. aeruginosa Ch1 to induce resistance of the plants to CMV was suggested by producing siderophores under the limited Fe conditions,17-20 ppm.