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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Microbiology Indonesia Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Jurnal Bakti Masyarakat Indonesia

DIANA ELIZABETH WATURANGI

Universitas Katolik Indonesia Atma Jaya

Author-ID : 413544

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Social Sciences Other

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Isolation and Identification of Ice-Nucleating-Active Bacteria from Indonesian Edible Leafy Plant Poh-Pohan (Pilea glaberina) DIANA ELIZABETH WATURANGI; VICKY MEICY; ANTONIUS SUWANTO
Microbiology Indonesia Vol. 2 No. 1 (2008): April 2008
Publisher : Indonesian Society for microbiology

Two ice-nucleating-active (INA) bacteria (isolates C and 6) were isolated from poh-pohan (Pilea glaberina), an Indonesian edible leafy plant (lalaban). The maximum nucleation temperature of aqueous suspensions of the two isolates is -5 °C. They were classified as a type II ice nucleator. Microscopic and morphological determination showed that these isolates had yellow pigmentation, rod shape, and were Gram negative. Biochemical analysis indicated that the isolates were exhibited catalase activity, but negative in oxidase and indole assays. DNA sequencing of 16SrRNA gene of isolate A3 showed a 94% similarity to Pseudomonas sp. while isolate A4 showed a 97% similarity to Xanthomonas campestris. To our knowledge, this is the first report of INA bacteria isolated from a tropical edible leafy plant.

Identification of Class 1 Integron of Escherichia coli from Street Foods in Jakarta . FRISCA; BIBIANA WIDYATI LAY; DIANA ELIZABETH WATURANGI
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

A total of 43 Escherichia coli isolates were identified from school street foods located in Northern and Southern Jakarta. The isolates were examined for antibiotic resistance using five antibiotics discs (ampicillin, kanamycin, streptomycin, trimethoprim, tetracycline) and screened for the class 1 integron with specific conserved region primer using PCR amplification. The antibiotic diffusion test revealed three isolates (7%) with resistance to multiple antibiotics. PCR detection of integron regions showed one isolate possessed a class 1 integron bearing one gene cassette with ~700bp amplicon size. DNA sequencing showed that the gene cassette was resistant to trimethoprim determinant type V (dhfrV). The integron bearing E. coli strain could become a threat for the widespread distribution of an antibiotic resistance gene especially for pathogenic bacteria.

Analysis of Pink Pigmented Facultative Methylotroph Bacteria from Human Environments DIANA ELIZABETH WATURANGI; ANDREAS KUSUMA
Microbiology Indonesia Vol. 2 No. 3 (2008): December 2008
Publisher : Indonesian Society for microbiology

The formation of pink biofilm in wet places are usually correlated with chlorine-resistant pink pigmented facultative methylotrophs (PPFM). In this study we investigated the presence of PPFM bacteria through bacterial isolation and detection of mxaF gene from wet places of human-made environments. A total of eighteen PPFM bacterial isolates were recovered from the formation of biofilm bacterial of four test places such as washstands, bathrooms, and potable water supplies. Confirmation of the isolates through biochemical analysis were done using catalase, oxidase and urease tests. Chlorine-resistance-activity was assayed for all of the isolates. Antibiotic resistance were examined for ampicillin (25 μg), tetracycline (30 μg), kanamycin (30 μg), trimethoprim (1.25 μg), and streptomycin (10 μg) using the agar diffusion method. Genomic DNA was subjected to PCR analysis with primers corresponding to the 5’- and 3’- end conserved segments of the mxaF gene. PCR amplification followed by DNA sequencing of 16S rRNA gene were done for some isolates. We recovered 18 isolates of PPFM bacteria. Biochemical analysis indicated that the isolates were positive for catalase, oxidase, and urease activities. Chlorine-resistance-analysis showed the majority of the isolates were resistant to chlorine. Antibiotic resistance assays showed all of the isolates exhibited resistance to trimethoprim but were sensitive to streptomycin, kanamycin, and tetracycline but were variably resistant to ampicilin. PCR detection using specific primers for the mxaF gene gave a positive result for all of the isolates. DNA sequencing of the 16S rRNA gene of two isolates showed that isolate WD10 had a 98% similarity with the mxaF gene from Methylobacterium lusitanum strain MP2 and isolate WK2 had a 98% similarity to the mxaF gene from Afipia felis strain RD1. The formation of pink biofilm of four wet areas in this study were correlated with the presence of chlorine-resistant PPFM bacteria and we confirmed with the presence of the mxaF gene in all of the isolates. This finding needs to be widely publicized since some PPFM bacteria were known as opportunistic pathogens.

Screening of Antibiofilm Activity from Marine Bacteria against Pathogenic Bacteria ALIANDA BUDHIRIANi ROSSATI CAMESI; AGUSTINA LUKITO; DIANA ELIZABETH WATURANGI; HWANG JAE KWAN
Microbiology Indonesia Vol. 10 No. 3 (2016): September 2016
Publisher : Indonesian Society for microbiology

Bacterial biofilms produced by pathogenic bacteria have become a serious issue in several chronic diseases such as atherosclerosis, cystic fibrosis, endocarditis, inner ear infections, and kidney stones. Thus, inhibition and destruction of bacterial biofilm from pathogenic bacteria is needed. The purpose of this study is to analyze biofilm inhibition and destruction activities of marine bacteria associated with hard and soft corals isolated from several oceanic regions in Indonesia. Fifteen marine isolates collected from several regions in Indonesia such as Bali Province, South East Sulawesi Province, East Java Province, Lampung Province, and Banten Province were tested using static biofilm assay against several pathogenic bacteria. Biofilm of the pathogenic bacteria tested were stained using 0.4% crystal violet. Several isolates were sequenced using 16S rRNA PCR method. Most of marine isolates presented higher inhibition and or destruction activity at 10% crude concentration. Few isolates were further identified using 16S rRNA and proven to have antibiofilm activity against several pathogenic bacteria. Marine bacteria have broad applications in medical and pharmaceutical industries and the oceanic regions of Indonesia are promising sources for the discovery of novel bacteria with antibiofilm activity.

Co-Authors . FRISCA . STEPHANIE ADI YULANDI AGUSTINA LUKITO ALIANDA BUDHIRIANi ROSSATI CAMESI AMELIA TJHEN ANDREAS KUSUMA Antonius Suwanto Anugrah, Daru Seto Bagus BIBIANA WIDYATI LAY CATHERINE DELANY NICHOLAS CINDY OKTAVIA SUSANTO Diana Lestari Diana Lestari, Diana Dionysius Subali Faustina Audrey Agatha Gustilatov, Muhamad HWANG JAE KWAN IVANA FRANCISCA IVANA FRANCISCA Jessica Igna Amanda Julie Ekasari Julyantoro, Pande Gde Sasmita MAGGY THENAWIJAYA SUHARTONO NADIA DEASHINTA Rory Hutagalung Sabrina, Feliciana Stephanie Stephanie Vanessa, Valencia VICKY MEICY WIDANARNI WIDANARNI Wijaya, Laura Yogiara Yogiara

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