Garuda - Garba Rujukan Digital

The influence of biofloc system on Vibrio composition, the growth and the gut microvilli performance of the Pacific white shrimp Penaeus vannamei Gustilatov, Muhamad; Widanarni, Widanarni; Ekasari, Julie; Julyantoro, Pande Gde Sasmita; Waturangi, Diana Elizabeth
Jurnal Akuakultur Indonesia Vol. 23 No. 1 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.1.12-23

Biofloc technology has been shown to have a positive impact on shrimp culture by controlling pathogenic Vibrio. This study aimed to analyze the effect of biofloc on the Vibrio composition in water and shrimp gut, as well as the growth performance and microvilli of Pacific white shrimp (Penaeus vannamei). Shrimp post-larvae measuring 0.66 ± 0.02 g were reared in a glass aquarium (working volume 22 L) of 6 units with a density of 150 shrimp m-3 for 28 days. The treatments included rearing in the biofloc system with C/N ratio 10, and without a biofloc system as a control. The results showed that Vibrio was highly prevalent in the control gut (4.13%) and biofloc water (3.77%), but only a few were found in the control water (0.16%) and biofloc-treated gut (0.11%). V. hepatarius (1.10%) and V. nereis (1.06%) were found to dominate the Vibrio bacterial community in the biofloc system maintenance media, while Vibrio sp. Hep-1b-8 (2.26%) and V. parahaemolyticus (0.80%) known as a pathogenic bacteria dominated the control shrimp gut. The biofloc system significantly increased the digestive enzyme activity, growth performance, and microvilli length in the shrimp gut. In conclusion, the application of a biofloc system in shrimp culture can affect the composition and abundance of bacterial communities in both the culture environment and the shrimp gut, and improve growth performance with higher digestive enzyme activity and longer microvilli in the gut. Keywords: biocontrol, biofloc, microbiota, shrimp, Vibrio ABSTRAK Teknologi bioflok menunjukkan dampak positif pada kegiatan budidaya udang vaname dengan mengendalikan bakteri patogen salah satunya Vibrio. Penelitian ini bertujuan untuk menganalisis efek bioflok terhadap komposisi Vibrio pada air dan usus udang, serta kinerja pertumbuhan dan mikrovili udang vaname (Penaeus vanamei). Udang post-larva berukuran 0.66 ± 0.02 g dipelihara pada akuarium kaca (volume air 22 L) sebanyak 6 unit dengan kepadatan 150 ekor m-3 selama 28 hari. Perlakuan meliputi pemeliharaan udang pada sistem bioflok dengan rasio C/N 10, dan tanpa sistem bioflok sebagai perlakuan kontrol. Hasil pengamatan menunjukkan kelimpahan Vibrio yang cukup tinggi pada usus perlakuan kontrol (4.13%) dan air perlakuan bioflok (3.77%), tetapi sangat sedikit ditemukan pada air perlakuan kontrol dan usus perlakuan bioflok. Selanjutnya, V. hepatarius (2.26%) dan V. nereis (1.06%) terdeteksi mendominasi komunitas bakteri Vibrio di media pemeliharaan pada sistem bioflok, sedangkan Vibrio sp. Hep-1b-8 (2.26%) dan V. parahaemolyticus (0.80%) mendominasi usus udang vaname pada perlakuan kontrol. Sistem bioflok juga mampu meningkatkan aktivitas enzim pencernaan, performa pertumbuhan, dan panjang mikrovili usus secara signifikan (P < 0.05). Kesimpulan dari penelitian ini adalah penerapan sistem bioflok pada budidaya udang vaname mampu memengaruhi komposisi dan kelimpahan komunitas bakteri Vibrio pada lingkungan budidaya maupun pada usus udang, serta meningkatkan performa pertumbuhan dengan aktivitas enzim pencernaan yang lebih baik dan mikrovilli usus yang lebih panjang. Kata kunci: bioflok, biokontrol, microbiota, udang vaname, Vibrio

Intensity and Prevalence of Ectoparasites in Tilapia (Oreochromis niloticus) from Floating Net Cages in Lake Batur, Bali Yogi, Gusti Ayu Putu Cintya Pratami; Julyantoro, Pande Gde Sasmita; Pebriani, Dewa Ayu Angga; Sudaryatma, Putu Eka
Journal of Fish Health Vol. 5 No. 2 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i2.6321

Tilapia (Oreochromis niloticus) is one of the most cultivated freshwater fish. The problem that is often faced in fish farming is diseases caused by parasitic attacks (ectoparasites) which can cause a decrease in fish production levels. To overcome these problems, research is needed on the intensity and prevalence of ectoparasites in tilapia (Oreochromis niloticus) to determine the density of ectoparasite attacks and the percentage of ectoparasites in tilapia. Tilapia (Oreochromis niloticus) samples were taken from the Floating Net Cage (KJA) of Lake Batur, Songan Village, Kedisan and Trunyan. Sampling is carried out by purposive sampling or determining location points. The tilapia (Oreochromis niloticus) samples examined were 36 fish samples with a size of 14.7-21 cm tilapia niwana. The ectoparasite found only in the gills of tilapia, namely Dactylogyrus sp. (506 ind) and Gyrodactylus sp. (13 ind). The highest intensity in the genus Dactylogyrus was 14.06 ind/head in the medium category, while in the genus Gyrodactylus it was 1.86 ind/head in the low category. The highest prevalence value in the genus Dactylogyrus is 100% with the always category while for the genus Gyrodactylus is 19.44% with the frequent category. The data supporting water quality in this study includes temperature, pH, and DO. The temperature of lake water in floating net cages during the study ranged from 23oC-28oC, pH or acidity degree ranged from 8.1-9 and DO range from 4.8-5.6 mg/L. The results of the water quality in this study are classified as optimal for tilapia rearing.

PENGARUH PERBEDAAN DOSIS PELET TEPUNG DAUN KELOR TERHADAP PERTUMBUHAN, KELULUSHIDUPAN, DAN HISTOLOGI USUS DALAM PEMBERIAN PAKAN IKAN NILA (Oreochromis niloticus) Gunawan, Sahrul; Julyantoro, Pande Gde Sasmita; Pebriani, Dewa Ayu Angga
Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan Vol 23 No 1 (2025): Jurnal Agroqua
Publisher : University of Prof. Dr. Hazairin, SH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32663/ja.v23i1.5108

This purpose of this study was to determine the best dose and the effect of moringa leaf pellets on the growth, survival, and intestinal histology of tilapia (Oreochromis niloticus). The research was conducted in January-March 2025 at the Cultivation Laboratory of the Faculty of Marine and Fisheries, Udayana University, and the Denpasar Veterinary Center. The method used was a Completely Randomized Design (CRD) with 4 treatments and 3 replications, using Moringa leaf pellet doses as follows: A (0%), B (25%), C (50%), and D (75%). Parameters observed included absolute weight and length growth, survival rate, feed conversion ratio (FCR), intestinal villus length, and water quality. The results showed no significant differences (P>0.05) in growth, survival, or FCR among treatments. However, intestinal villus length differed significantly (P<0.05). Water quality parameters during the study were within the limits set by Government Regulation of the Republic of Indonesia No. 22 of 2020, temperature ranging from 27.4–27.5 ℃, dissolved oxygen (DO) from 6.7–6.8 mg/L, pH from 8.10–8.17, and ammonia levels below 0.50 mg/L.

MINIREVIEW ON SUSTAINABLE ANTIVIRULENCE STRATEGY FOR AQUACULTURE Julyantoro, Pande Gde Sasmita; Bossier, Peter; Defoirdt, Tom
Jurnal Riset Akuakultur Vol 19, No 2 (2024): Juni (2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.2.2024.157-176

The increasing occurrence of antibiotic-resistant bacteria is one of the major challenges currently faced by the aquaculture sector. Ineffective applications of antibiotics to treat bacterial diseases, leading to the need for alternative strategies to address the problem. The antivirulence approach is a highly promising strategy that aims to stop pathogenic bacteria from causing harm to the host by disrupting their virulence mechanisms. This approach involves understanding the mechanisms of bacterial pathogenicity that can be developed into new therapeutic methods. There have been numerous advancements in combating bacterial infections, such as disrupting host-pathogen communication and inhibiting quorum sensing (QS). Antivirulence therapy offers a significant advantage as it specifically targets bacterial virulence without imposing excessive pressure on bacterial growth, reducing the risk of resistance development. This review outlines the limitations of antibiotic use and presents current insights into bacterial pathogenicity mechanisms and antivirulence strategies in aquaculture. It particularly highlights the impact of host-pathogen signaling via catecholamines, stress hormones, and QS mechanisms in certain aquaculture-pathogenic bacteria. The influence of host stress hormones on pathogen growth and virulence is noteworthy. Quorum sensing (QS) is known to regulate the expression of certain virulence genes in response to bacterial density by releasing and detecting a small signal molecule called autoinducers. This review further explains various strategies to interfere with QS mechanisms, including inhibiting signal molecule biosynthesis, using QS antagonists, chemical inactivation, or biodegradation of QS signals. These promising strategies have been considered as the first step and proof of concept of antivirulence strategies to prevent disease outbreaks in aquaculture.Meningkatnya jumlah bakteri yang resisten terhadap antibiotik merupakan salah satu tantangan besar yang saat ini dihadapi oleh sektor akuakultur. Penerapan antibiotik yang tidak efektif untuk mengobati penyakit bakterial, menyebabkan perlunya strategi alternatif untuk mengatasi masalah tersebut. Pendekatan antivirulensi adalah strategi yang sangat menjanjikan yang bertujuan untuk menghentikan bakteri patogen dalam menyebabkan kerusakan pada inang dengan mengganggu mekanisme virulensinya. Pendekatan ini melibatkan pemahaman mekanisme patogenisitas bakteri yang dapat dikembangkan menjadi metode terapi baru. Terdapat banyak perkembangan dalam melawan infeksi bakteri, seperti mengganggu komunikasi inang-patogen dan menghambat quorum sensing (QS). Terapi antivirulensi menawarkan keuntungan yang signifikan karena secara spesifik menargetkan virulensi bakteri tanpa memberikan tekanan berlebihan pada pertumbuhan bakteri, sehingga mengurangi risiko berkembangnya resistensi. Reviu ini menguraikan keterbatasan penggunaan antibiotik dan menyajikan wawasan terkini mengenai mekanisme patogenisitas bakteri dan strategi antivirulensi dalam budidaya perikanan. Reviu ini terutama menyoroti dampak sinyal patogen inang melalui katekolamin, hormon stres, dan mekanisme QS pada bakteri patogen tertentu dalam akuakultur. Pengaruh hormon stres inang terhadap pertumbuhan dan virulensi patogen patut diperhatikan. Quorum sensing (QS) diketahui mengatur ekspresi gen virulensi tertentu sebagai respons terhadap kepadatan bakteri dengan melepaskan dan mendeteksi molekul sinyal kecil yang disebut autoinduser. Reviu ini lebih lanjut menjelaskan berbagai strategi untuk mengganggu mekanisme QS, termasuk menghambat biosintesis molekul sinyal, menggunakan antagonis QS, inaktivasi kimia, atau biodegradasi sinyal QS. Strategi yang menjanjikan ini telah dianggap sebagai langkah pertama dan bukti dari konsep strategi antivirulensi untuk mencegah wabah penyakit pada budidaya perikanan.

KESESUAIAN EKOLOGI BUDIDAYA UDANG VANAME (Litopenaeus vannamei) PADA TAMBAK SEMI INTENSIF DI KECAMATAN GEROKGAK, BALI Sari, Putu Dewi Purnama; Arthana, I Wayan; Julyantoro, Pande Gde Sasmita
Jurnal Riset Akuakultur Vol 17, No 2 (2022): (Juni) 2022
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.17.2.2022.121-132

Kecamatan Gerokgak menjadi salah satu pusat perikanan budidaya, khususnya budidaya udang vaname. Tujuan dari penelitian ini adalah untuk menentukan parameter kesesuaian ekologi pada kegiatan budidaya udang vaname di Kecamatan Gerokgak, Bali. Penelitian dilaksanakan di enam petak tambak yang berada di tiga desa, yaitu Desa Gerokgak, Patas, dan Sanggalangit mulai bulan Oktober 2021 hingga Januari 2022. Parameter ekologi yang diamati adalah suhu, pH, salinitas, oksigen terlarut, kecerahan, alkalinitas, amonia, bahan organik total, nitrat, nitrit, fosfat, kelimpahan plankton, bakteri heterotrofik, dan Vibrio. Data dianalisis menggunakan matriks kesesuaian dan dihitung indeks kesesuaiannya. Hasil penelitian menunjukkan suhu pagi sebesar 24,0-26,8°C; suhu sore 27,0-30,4°C; pH 7,3-8,1; salinitas 6-33 ppt; oksigen terlarut 7,7-7,9 mg L-1; kecerahan 38,5-44,6 cm; alkalinitas 205,9-212,6 mg L-1; amonia 0,28-0,48 mg L-1; bahan organik total 30-33 mg L-1; nitrat 0,2-0,3 mg L-1; nitrit 0,01-0,02 mg L-1; fosfat 0,53-0,66 mg L-1; kelimpahan plankton 117.909-200.764 ind L-1; bakteri heterotrofik 1,8x105-2,4x105 CFU mL-1; dan Vibrio sebesar 2.776-3.620 CFU mL-1. Semua tambak menunjukkan tingkat sangat sesuai untuk budidaya udang vaname. Nilai indeks kesesuaian tertinggi terdapat pada petak Desa Sanggalangit 2 (95,9%), diikuti oleh Desa Sanggalangit 1 dan Desa Patas 1 (93,8%), sementara nilai Desa Patas 2, Desa Gerokgak 1dan Desa Gerokgak 2 mendapatkan nilai indeks kesesuaian yang terendah (91,8%). Penelitian ini menyimpulkan bahwa seluruh petak yang diamati termasuk dalam tingkat kesesuaian ekologi Sangat Sesuai untuk budidaya udang vaname.Gerokgak area is the primary coastal aquaculture center in North Bali in which whiteleg shrimp farming has grown rapidly in the past decade. This study aimed to determine the ecological suitability of semi intensive whiteleg shrimp farming in the area. The research was carried out in six different ponds in three villages (Gerokgak, Patas, and Sanggalangit Villages) from October 2021 to January 2022. The evaluation parameters consist of temperature, pH, salinity, dissolved oxygen, transparency, alkalinity, ammonia, total organic matter, nitrate, nitrite, phosphate, plankton abundance, heterotroph bacteria count, and Vibrio count. The collected data were arranged into suitability matrix from which the suitability indexes were calculated. The morning temperature was 24.0-26.8°C; afternoon temperature 27.0-30.4°C; pH 7.3-8.1; salinity 26-33 ppt; dissolved oxygen 7.7-7.9 mg L-1; brightness 38.5-44.6 cm; alkalinity 205.9-212.6 mg L-1; ammonia 0.28-0.48 mg L-1; total organic matter 30-33 mg L-1; nitrate 0.2-0.3 mg L-1; nitrite 0.01-0.02 mg L-1; phosphate 0.53-0.66 mg L-1; plankton abundance 117,909-200,764 ind L-1; heterotrophic bacteria 1.8x105-2.4x105 CFU mL-1; and Vibrio 2,776–3,620 CFU mL-1. The highest suitability index value was scored by Sanggalangit Village pond 2 (95.9%), followed by Sanggalangit Village pond 1 and Patas Village pond 1 (93.8%), while Patas Village pond 2, Gerokgak Village pond 1, and Gerokgak Village pond 2 had the lowest suitability index (91.8%). This study concludes that all observed ponds are categorized as highly ecologically suitable for whiteleg shrimp culture.

Intensity and Prevalence of Ectoparasites in Tilapia (Oreochromis niloticus) from Floating Net Cages in Lake Batur, Bali Yogi, Gusti Ayu Putu Cintya Pratami; Julyantoro, Pande Gde Sasmita; Pebriani, Dewa Ayu Angga; Sudaryatma, Putu Eka
Journal of Fish Health Vol. 5 No. 2 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i2.6321

Tilapia (Oreochromis niloticus) is one of the most cultivated freshwater fish. The problem that is often faced in fish farming is diseases caused by parasitic attacks (ectoparasites) which can cause a decrease in fish production levels. To overcome these problems, research is needed on the intensity and prevalence of ectoparasites in tilapia (Oreochromis niloticus) to determine the density of ectoparasite attacks and the percentage of ectoparasites in tilapia. Tilapia (Oreochromis niloticus) samples were taken from the Floating Net Cage (KJA) of Lake Batur, Songan Village, Kedisan and Trunyan. Sampling is carried out by purposive sampling or determining location points. The tilapia (Oreochromis niloticus) samples examined were 36 fish samples with a size of 14.7-21 cm tilapia niwana. The ectoparasite found only in the gills of tilapia, namely Dactylogyrus sp. (506 ind) and Gyrodactylus sp. (13 ind). The highest intensity in the genus Dactylogyrus was 14.06 ind/head in the medium category, while in the genus Gyrodactylus it was 1.86 ind/head in the low category. The highest prevalence value in the genus Dactylogyrus is 100% with the always category while for the genus Gyrodactylus is 19.44% with the frequent category. The data supporting water quality in this study includes temperature, pH, and DO. The temperature of lake water in floating net cages during the study ranged from 23oC-28oC, pH or acidity degree ranged from 8.1-9 and DO range from 4.8-5.6 mg/L. The results of the water quality in this study are classified as optimal for tilapia rearing.

Title

Found 6 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 6 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 6 Documents
Search