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PENGARUH MELATONIN TERHADAP KADAR ASAM LAKTAT PADA TIKUS WISTAR MODEL SEPSIS Diana Kumalasari; Satrio Adi Wicaksono
Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal) Vol 5, No 4 (2016): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Latar Belakang : Melatonin merupakan radikal bebas yang sering digunakan sebagai antioksidan. Melatonin merupakan salah satu obat yang sedang dikembangkan sebagai terapi sepsis. Toksisitas serius akibat pemberian melatonin tidak muncul pada pemakaian dosis tinggi. Sepsis menyebabkan peningkatan kadar asam laktat melalui glikolisis anaerob yang terjadi akibat hipoksia jaringan. Melatonin sangat efektif digunakan pada keadaan tersebut, dimana melatonin dapat menurunkan kadar asam laktat sehingga mencegah terjadinya kematian.Tujuan : Mengetahui pengaruh melatonin terhadap kadar asam laktat pada tikus wistar model sepsis dan memperoleh informasi tentang melatonin dalam menurunkan kadar asam laktat.Metode : Penelitian ini merupakan penelitian eksperimental dengan desain Randomized Control Group Pre-Post Test. Sampel adalah 12 ekor tikus wistar jantan dengan kriteria tertentu, dibagi secara acak menjadi 2 kelompok. Kelompok I sebagai kelompok kontrol diberi injeksi Lipopolisakarida (LPS) intraperitoneal dan tidak diberi melatonin. Kelompok II sebagai kelompok perlakuan diberi injeksi LPS intraperitoneal dan melatonin via sonde oral. Setelah tikus diadaptasi selama 7 hari, pada hari ke-8 tikus diinjeksi LPS pada kedua kelompok dan melatonin via sonde oral hanya pada kelompok perlakuan. Kemudian tiap tikus diambil darahnya melalui pembuluh darah retroorbita dan diukur kadar asam laktatnya. Uji statistik menggunakan uji Paired t-Test, Independent t-Test dan Mann-Whitney Rank Test.Hasil : Pada uji Independent t-Test didapatkan nilai rerata kadar asam laktat pada kelompok kontrol lebih tinggi dibanding dengan kelompok perlakuan. Pada uji Paired t-Test tidak didapatkan perbedaan yang signifikan p > 0,05 pada kelompok kontrol maupun perlakuan kecuali pada post1-post 2 dan pre-post 2 yang memiliki perbedaan yang signifikan pada kelompok kontrol. Pada uji Mann-Whitney Rank Test juga tidak didapatkan perbedaan yang signifikan pada selisih pre – post 1 dan selisih pre – post 2.Kesimpulan : Pemberian melatonin tidak menyebabkan penurunan kadar asam laktat yang signifikan.

PENGARUH MELATONIN TERHADAP JUMLAH LEUKOSIT PADA TIKUS WISTAR MODEL SEPSIS Rizqi Indah Riani; Satrio Adi Wicaksono
Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal) Vol 5, No 4 (2016): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Latar Belakang Melatonin merupakan radikal bebas yang sering digunakan sebagai antioksidan. Melatonin berperan dalam meningkatkan respon imun, dan membantu proses sitoprotektif. Dalam beberapa model hewan, melatonin telah diidentifikasi untuk membantu melawan infeksi yang disebabkan bakteri, virus, dan parasit dengan melalui berbagai mekanisme, seperti immunomodulasi atau aktivitas antioksidan. Melatonin dapat mengurangi kadar sitokin inflamasi, stress oksidatif dan disfungsi mitokondria. Melatonin merupakan salah satu obat yang dikembangkan sebagai terapi sepsis.Tujuan Mengetahui pengaruh melatonin terhadap jumlah leukosit pada tikus wistar model sepsis dan memperoleh informasi melatonin dapat menurunkan jumlah leukosit.Metode Penelitian ini adalah penelitian eksperimental dengan rancangan randomized control grup pre post test . Sampel adalah 12 ekor tikus wistar jantan dengan kriteria tertentu, dibagi secara acak menjadi 2 kelompok. Kelompok I diberi injeksi intraperitoneal lipopolisakarida (LPS) dan tidak diberi melatonin sebagai kelompok kontrol, sedangkan kelompok II diberi injeksi intraperitoneal lipopolisakarida (LPS) sebagai kelompok perlakuan dan diberi melatonin via sonde oral sebagai kelompok perlakuan. Setelah adaptasi tikus selama seminggu, pada hari ke 8 tikus diambil darahnya melalui pembuluh darah retroorbita. Uji statistik menggunakan uji paired t-test, independent t-test dan Mann Whitney Test.Hasil Pada uji independent test didapatkan nilai rerata jumlah leukosit pada kelompok kontrol lebih tinggi dibanding kelompok perlakuan. Pada uji paired t-test kelompok kontrol mengalami perubahan yang signifikan (p<0,05) dibandingkan dengan kelompok perlakuan yang menunjukkan hasil yang tidak bermakna. Pada uji Mann Whitney Test didapatkan hasil kelompok kontrol selisih pre– post1 dan post 2 mengalami peningkatan yang signifikan. Sedangkan pada kelompok perlakuan selisih pre LPS – post1 dan post2 (p<0,05) mengalami penurunan yang signifikan.Kesimpulan Pemberian melatonin tidak menyebabkan penurunan jumlah leukosit yang signifikan.

THE EFFECT OF PARACETAMOL AND CODEINE ANALGESIC COMBINATION ON SERUM GLUTAMIC OXALOACETATE TRANSAMINASE LEVELS IN MALE WISTAR RATS Rona Ayu Hanifah; Farah Hendara Ningrum; Erwin Kresnoadi; Satrio Adi Wicaksono
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 9, No 2 (2020): DIPONEGORO MEDICAL JOURNAL ( Jurnal Kedokteran Diponegoro )
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Introduction : Paracetamol is an effective analgesic to relieve mild to moderate pain when it is used in therapeutic doses. Codeine is an opioid analgesic to relieve moderate to severe pain. Both are metabolized in the liver and have different mechanisms in the treatment of pain. The use of paracetamol and codeine as monotherapy has been extensive, but research on the effectiveness of these drugs in combination is still limited, especially about its effect in liver damage. This study was to investigate the effect of paracetamol and codeine analgesic combination Serum Glutamic Oxaloacetate Transaminase levels in male Wistar rats. Method : This was an experimental study using Post-Test Only Control Group Design. The samples were 24 male wistar rats randomized into 4 groups; group I (control group, without treatment), group II receiving paracetamol 32 mg/kgBB, group III receiving codeine 1,9 mg/kgBB, and group IV receiving combination of paracetamol 32 mg/kgBB and codeine 1,9 mg/kgBB. Drugs were administered through oral gastric instillation 4 times a day for 28 days. Blood samples were collected at the 29th day through retroorbital vessel to measure the SGOT levels. The data was analysed using One-Way ANOVA test and Post-Hoc test. Results : The results of this research were obtained from statistical tests where there was no significant increase of the levels of Serum Glutamic Oxaloacetate Transaminase of Wistar rats which received a combination of paracetamol and codeine in the control group (p = 0.005). While in the other group there was not significant differences of the levels of Serum Glutamic Oxaloacetate Transaminase. Conclusion : There is no significant difference of Serum Glutamic Oxaloacetate Transaminase levels between the administration of paracetamol and codeine combination compared to the control group.Keywords : Paracetamol, codeine, paracetamol and codeine combination, SGOT levels, pain

The Comparison between Preoperative Maltodextrine and Sugar Water Administration on Random Blood Glucose Levels in Enhanced Recovery After Cesarean Surgery (ERACS) Patients Wicaksono, Satrio Adi; Pudjonarko, Dwi; Riwanto, Ignatius
JAI (Jurnal Anestesiologi Indonesia) Vol 16, No 3 (2024): JAI (Jurnal Anestesiologi Indonesia)
Publisher : Perhimpunan Dokter Spesialis Anestesiologi dan Terapi Intensif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jai.v0i0.65043

Background: Oral carbohydrate administration reduces post-operative insulin resistance and improves post-operative recovery. Sugar water and maltodextrin are oral carbohydrates that can be given to enhanced recovery after caesarean surgery (ERACS) patients.Objective: To compare the effect between pre-operative maltodextrin treatment and sugar water treatment on random blood glucose (RBG) levels in enhanced recovery after caesarean section (ERACS) patients.Methods: A randomized control study with pre-test and post-test design was used on 48 patients who underwent ERACS surgery at Dr. Kariadi hospital Semarang Indonesia and met the inclusion and exclusion criteria. Study subjects were divided into two groups: a pre-operative maltodextrin treatment group (n=24) and a pre-operative sugar water control group (n=24). RBG levels were checked with point-of-care testing (POCT) at soon before surgery and at 2 hours after surgery. Mann-Whitney test was used to analyse the difference of RBG levels between group with Maltodextrin and group with sugar water. Wilcoxon test was used to analyse the difference of RBG levels before surgery (pre-operative RBG) and 2 hours after surgery (post-operative RBG). The p value of <0.05 was considered as statistically significant.Result: There were significant lower RBG levels in group with pre-operative maltodextrin treatment compared to group with pre-operative sugar water at soon before surgery (pre-operative) (83.5 ± 9.73 vs 96.2 ± 12.99 mg/dL, p=0.003) and at 2 hours post-operative (101.7 ± 15.81 vs 118.9 ± 28.38, p=0.035) in ERACS patients.Conclusion: If confirmed by further studies, pre-operative maltodextrin administration might provide better outcome in reducing post-operative catabolic status by reducing post-operative insulin resistance and improving RBG levels before and after ERACS compared to sugar water control.

The increasing of Human Resources quality Through Food Security in Semarang City (A Study of Legal Anthropology) Handayani, Emy; Wicaksono, Satrio Adi
International Journal of Multidisciplinary: Applied Business and Education Research Vol. 2 No. 11 (2021): International Journal of Multidisciplinary: Applied Business and Education Res
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/10.11594/ijmaber.02.11.17

Development of food security has a strategic meaning in development in an area through its human resources, in accordance with Constitution number 18 of 2012 concerning food, that the provincial, district / city and / or village governments determine the type and amount of certain food reserves according to the consumption needs of the local community. The Human Development Index (HDI) also states that three factors determine the Human Development Index, namely education, health, and economy, so the quality of human resources is very influential and has an important role in developing and managing food so that a synergistic relationship between various aspects of the food security development in a region is needed. In this study, improving the quality of human resources in the city of Semarang in terms of the Anthropological Study of Law on food security, the method used through a normative juridical approach that is the law conceptualized as a normative symptom in legislation with the legal anthropological approach, the population in this study is the Department of Agriculture Semarang City with samples of food products in improving the quality of its human resources The results of this study comes through the Empirical Legal Anthropology approach, the support of strong and effective food institutions can spur growth, development and improvement of food, the nutritional status improvement of the community is very influential in improving the quality of human resources in increasing intelligence and the performance of human resources in determining future growth as one of strengthening local food security in the city of Semarang. With the availability of high quality human resources characterized by a strong physical, strong mental, excellent health and mastering science and technology can support the development of the city of Semarang.

Existence of Sobokartti Building as A Physical Culture in Semarang City in Law Anthropology Approach Handayani, Emy; Wicaksono, Satrio Adi
International Journal of Multidisciplinary: Applied Business and Education Research Vol. 2 No. 11 (2021): International Journal of Multidisciplinary: Applied Business and Education Res
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/10.11594/ijmaber.02.11.16

Physical culture can be understood as objects created by human beings which are an embodiment of the growth and development of cultural values in a particular society, in the form of buildings that have been designated as cultural heritage buildings in the city of Semarang. The building is the Semarang Sobokartti building on Jalan Dr. Cipto Semarang, which is a reflection of the development of humans to always preserve culture both Javanese culture and western or European culture The approach used in this research is an empirical approach and a comparative approach to law anthropology. In an empirical approach, the sobokartti building is a physical culture which is a historic building that accommodates traditional arts which are closely related to Javanese culture, namely karawitan training, puppetry routine, puppetry courses, host courses, dancing courses and batik training. It should be appreciated because the community can maintain its preservation until now by showing the existence of Javanese culture with a love for culture that deserves to be preserved and for the nation's successor generation. Likewise with a comparative approach, it is said that the existence of physical culture in the sobokartti building is expected by the government to have regulations on cultural heritage objects, namely historic buildings or ancient buildings of the Sobokartti Semarang building as outlined in the Cultural Heritage Law No. 11 of 2010 to protect cultural heritage buildings with the aim of preserving, utilizing, and maintaining the beauty of a cultural heritage building Sobokartti Semarang. Suggestions that will be used in this research that is the role of the Government and Society to maintain physical cultural wealth should be developed by the nation's next generation, with the utilization of the Sobokartti building can provide sustainable use. So that, the Government and the community have the desire to protect and preserve the function of the Sobokartti Semarang building.

Efek Perbedaan Ventilasi Mekanik Positive End Expiratory Pressure (PEEP) Low dan Moderate terhadap Rasio PaO2/FiO2 Pascabedah pada Kraniotomi Elektif Rizki, Muhammad; Harahap, M. Sofyan; Wicaksono, Satrio Adi
Jurnal Neuroanestesi Indonesia Vol 9, No 3 (2020)
Publisher : https://snacc.org/wp-content/uploads/2019/fall/Intl-news3.html

Latar Belakang dan Tujuan: Kraniotomi elektif memiliki kejadian komplikasi paru pascaoperasi (25%) dan mortalitas (10%) yang tinggi. Penelitian ini berusaha mengetahui efek Positive End Expiratory Pressure (PEEP) 5 cmH2O and 8 cmH2O terhadap rasio PaO2/FiO2 pascaoperasi pada kraniotomi elektifSubjek dan Metode: Uji klinis acak ini dilakukan di satu rumah sakit pendidikan di Indonesia. Lima puluh dua pasien kraniotomi elektif (usia 17-55 tahun, lama bedah 4 jam, paru normal) dirandomisasi ke dalam 2 kelompok intervensi: ventilasi mekanik perioperatif dengan low Positive End Expiratory Pressure ( PEEP) (5 cmH2O) atau moderate PEEP (8 cmH2O). Hipotesis penelitian ini adalah rasio PaO2/FiO2 kelompok moderate PEEP lebih tinggi dibandingkan low PEEP. Analisis gas darah dilakukan pada 24 jam pasca induksi Hasil: Penelitian ini tidak menunjukkan perubahan yang signifikan rasio PaO2/FiO2 antara kelompok low PEEP dan moderate PEEP. Rasio PaO2/FiO2 kelompok low PEEP dan moderate PEEP secara berurutan adalah: pada 24 jam pasca induksi, 429,34 72,25 mmHg dan 458,59 71,11mmHg (p =0,147).Simpulan: Perbandingan low PEEP dan moderate PEEP pada ventilasi mekanik perioperatif tidak menghasilkan perbedaan nilai rasio PaO2/FiO2yang signifikan pada 24 jam pasca induksi.The Differential Effect of Low and Moderate Positive End Expiratory Pressure (PEEP) Mechanical Ventilation to Postoperative PaO2/FiO2 Ratio in Elective CraniotomyAbstractBackground and Objective: Elective craniotomy is associated with high incidence of postoperative pulmonary complications (PPC, 25%) and mortality (10%). We determined to study the effect of Positive End Expiratory Pressure (PEEP) 5 cmH2O and 8 cmH2O to postoperative PaO2 / FiO2 ratio (PF ratio) in elective craniotomy.Subject and Methods: This randomized clinical trial was at a university hospital in Indonesia. Fifty two elective craniotomy patients (ages 1755 years, surgical duration 4 hours, normal lung) were randomized into 2 intervention groups: perioperative mechanical ventilation with low PEEP (5 cmH2O) or moderate PEEP (8 cmH2O). The hypothesis of this study is that the ratio of PaO2 / FiO2 in the moderate PEEP group is higher than low PEEP. Blood gas analysis was performed 24 hours post induction.Results: This study did not show a significant difference in the PaO2/FiO2 ratio between the low PEEP and moderate PEEP groups. The PaO2 / FiO2 ratios of the low PEEP and moderate PEEP groups were respectively: at 24 hours post induction, 429.34 72.25 mmHg and 458.59 71.11mmHg (p = 0.147). Conclusions: Comparison of low PEEP and moderate PEEP in perioperative mechanical ventilation did not result in a significant difference in the value of the PaO2/FiO2 ratio at 24 hours post induction

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