Sri Widawati
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia, Jln. Raya Jakarta-Bogor Km. 46, Cibinong Science Center, Jawa Barat, Indonesia

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Journal : BERITA BIOLOGI

ISOLASI DAN AKTIVITAS PLANT GROWTH PROMOTING RHIZOBACTERIA (RHIZOBIUM, AZOSPIRILLUM, AZOTOBACTER, PSEUDOMONAS) DARI TANAH PERKEBUNAN KARET, LAMPUNG [Isolation And Activity Of Plant Growth Promoting Rhizobacteria (Rhizobium, Azospirillum, Azotobacter, Pseudomonas) From Soil Of Rubber Plantation, Lampung] Widawati, Sri
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (840.976 KB) | DOI: 10.14203/beritabiologi.v14i1.1868

Abstract

Analysis of phosphate dissolving activity, P dissolved; PMEase and IAA production by Plant Growth Promoting Rhizobacteria group(PGPR)is a parameter to determine the effectiveness of these bacteria as biological organic fertilizer (BOF).This study was aimed to obtain PGPR (Rhizobium, Azospirillum, Azotobacter, Pseudomonas) that were potential as a BOF to reclamation on degraded plantation area. This study used a random sampling method for 11 sampling points (1999P1, 1999P2, 1999P3, 1999P4, 1999P5, 2007P, 2007P1,2007P2, 2007P3, 2007P4, 2007P5) in the area of rubber plant roots.Isolation and counting of bacterial populations used plate count method on selective media (YEMA, Okon, Caceres, NFB AMA, PAB) whereas phosphate dissolving activity analysis, Dissolution Efficiency Index (DE), PMEase and IAA production following the method of Nguyen, Bray, Seshadri, Tabatabai, and Gravel.The results showed that of the 11 sampling points, 11 isolates were obtained effectively consisting of four groups of bacteria, namely: Rhizobium,5 5 5 5 Azospirillum, Azotobacter and Pseudomonas with the number of population: 3.3x10 , 80x10 , 20x10 , and 14x10 cfu.The results of the analysis of DE, soluble P, PMEase acid-base, and the highest IAA obtained from the bacterium isolated from the sampling point 1999P3 and 1999P1. This potential isolates will be used to the next research on reclamation of degraded land plantation
PENGARUH SALINITAS DAN INOKULAN BAKTERI TERHADAP PERTUMBUHAN TANAMAN TERUNG (Solanum melongena L.) [The Effect of Salinity and Bacteria Inoculant on The Growth of Eggplant (Solanum melongena L.)] Suliasih, Suliasih; Widawati, Sri
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3403.499 KB) | DOI: 10.14203/beritabiologi.v15i1.2854

Abstract

Nitrogen fixing dan phosphate solubilizing bacteria are important PGPR bacterial in addition of plant nutrients by increasing N and P, especially in saline soils. Experiments were conducted to observe the effects of  soil salinity and bacterial inoculant on growth eggplant (Solanum melongena) at green house of  Microbiology division, Research Center for Biology, The Indonesian Institute of Sciences,  Cibinong. Experiment laid out as factorial based randomized complete design  with three replications. Five levels of watering consisted of (1). Fresh water, (2). NaCl 0.1%, (3). NaCl 1%, (4). Sea water 75 %+25% fresh water, (5). Sea water 100%  as first factors. Five levels of inoculations/biofertilizers consisted of (1).control without fertilizer (K), (2). NPK (P), (3). Nitrogen Fixing bacteria (BPN1), all treatments was repeated 3 times  BPN2, mixed inoculation (BPN1 +  BPN2 + Phosphate Solubilizing Bacteria/BPF) as second factors.  Results showed that biofertilizer application increased growth and yield of eggplant in various levels of salinity at about 21.56%-53.35%  compared to uninoculated plants
THE POPULATION OF NITROGEN FIXING BACTERIA AND PHOSPHATE SOLUBILIZING BACTERIA IN THE RHIZOSPHERE FROM GUNUNG HALIMUN NATIONAL PARK Widawati, Sri; Suliasih, Suliasih
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.562 KB) | DOI: 10.14203/beritabiologi.v5i6.1075

Abstract

The biodiversity of nitrogen fixing bacteria (Rhizobium, Azotobacter and Azospirillum) and phosphate solubilizing bacteria in rhizosphere collected from Gunung Halimun National Park were studied.Bacterial population was counted from soil rhizosphere collected from various ecosystem types (determined based on elevation) namely 600 m, 1000 m, 1100 m, 1500 m and 1800 m above sea level.The results showed that the highest population of N-fixing and P solubilizing bacteria at Cikaniki (1100 asl) are founded in the plant rhizosphere of Schima wallichii in plot Suzuki A I and Altingia exelsa in Suzuki A III,at Gunung Botol area is in 1000 m asl,and Ciptarasa area is in 600 m asl.The population of N-fixing and P solubilizing bacteria at Gunung Halimun was influenced by the vegetation type, soil pH,and the elevation of area. Rhizobium, Azotobacter, Azospirillum (N-fixing bacteria); Pseudomonas sp, and Bacillus megaterium (P solubilizing bacteria), are most dominant at 600 m, 1000 m and 1100 m asl.