R Widiastuti
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Food Safety for Animal Products: The Need in The International Free Trade Bahri, Sjamsul; ., Indraningsih; Widiastuti, R; Murdiati, T B; Maryam, R
Indonesian Bulletin of Animal and Veterinary Sciences Vol 12, No 2 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.499 KB) | DOI: 10.14334/wartazoa.v12i2.767

Abstract

Issues of food safety, includes the food safety for the animal products grows to inhibit the incoming of low quality imported animal product which is unsafe for human being. Therefore several countries enhance to produce high quality of animal product and safe to be consumed. This paper reviews several factors that distribute to the quality and food safety in every single chain of the preparation of animal products. In pre-production step, many factors involve to the quality and safety because several contaminants and diseases can be happened in this step. Several data of diseases and contaminants which causes the negative impacts on economic, political and health will be provided in this paper. An effort to produce high quality and safe animal products can be done by implementing the HACCP in every step of preparation of it (GAP, GHP, GMP, GDP, and GRP). To assure the distribution of high quality and halal of imported animal products in Indonesia, it is necessary to give more strict regulation which is supported by infrastructure, facilities, human resources development and law enforcement.   Key words: Food safety, animal products, international free trade, HACCP
The residue of trenbolone from male Garut sheep which implanted by trenbolone acetate Widiastuti, R; ., Indraningsih; Murdiarti, T.B; Firmansyah, R
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 3 (2001)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.817 KB) | DOI: 10.14334/jitv.v6i3.233

Abstract

Trenbolone acetate (TBA) is a growth hormone promoter which is implanted into animal to increase the body weight. The implantation of TBA in animal may cause the occurrence of residues of TBA and its metabolite (17-β-trenbolone). The presence of the residue might threat to human health. The aim of this research work was to study the presence of the residue of trenbolone in male Garut sheep which is implanted by TBA. The sheep were divided into 2 groups, those were D1 which were implanted by 40 mg TBA and D2 which were implanted by 60 mg TBA. One animal each from D1 and D2 were killed in the week 1, 2, 3 and 4 after implantation. And 24 hours before slaughtering the urine of D1 and D2 were collected using the metabolism cages. The samples for residue analysis were the organs such as liver, kidney, and the muscles around the implantation sites i.e inner and outer, upper front leg and upper back leg. The samples were extracted by organic solvents and analysed by HPLC. The residue of trenbolone occured in all samples of D1 and D2 which killed on 1 and 2 weeks after implantation. The residues occured on those which were killed 3 weeks after implantation were only in inner and outer samples. The residues in meat and organs were not detected in D1 nor D2 which were killed 4 weeks after implantation. The residue of trenbolone was also detected in the urine and still were detected in D1 and D2 which implanted by TBA for 4 weeks.   Key words : trenbolone acetate, 17-β-trenbolone, residue, sheep
Aflatoxin contamination in feeds and feed ingredients from Lampung and East Java provinces Bahri, Sjamsul; Maryam, R; Widiastuti, R
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 3 (2005)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.67 KB) | DOI: 10.14334/jitv.v10i3.448

Abstract

In the last few years, poultry industries (broiler and laying hens) in Indonesia are faced on several problems, vaccination for instance, which suspected relate to highly-contaminated aflatoxins in feeds. The aim of this study is to give recent information on aflatoxin contamination in feeds, feed ingredients, and foods originated from some districts in Lampung and East Java provinces. The results of the study indicated that the percentage of aflatoxin B1 contamination in corn, commercial feeds, and peanuts in Lampung were 86.7, 70.0 and 80.0% with the average levels of 31.5, 13.5 and 32.2 μg/kg, respectively. Low levels of the other aflatoxins (AFB2, AFG1, and AFG2) were also detected in the samples. The highest percentage of aflatoxin contamination was found in samples from retailer (100%), followed by samples from traditional markets (91.7%), poultry shops (76.9), and a feed company (33.3%). The average levels of aflatoxin found were 58.8, 34.3, 17.8 and 2.9 μg/kg, respectively. In East Java, AFB1 was detected in all samples (corn, rice bran, concentrate and commercial feeds) on the average levels of 25.4, 69.7, 134.2 and 30.7 μg/kg respectively. The levels of AFG1 on the samples from East Java were much more higher than those of from Lampung, as well as the percentage.     Key Words: Aflatoxins, Feed, Feed Ingredients, Lampung, East Java
The effect of aflatoxins B1 (AFB1) on chick embryo Bahri, Sjamsul; Widiastuti, R; Mustikaningsih, Y
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 2 (2005)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (298.84 KB) | DOI: 10.14334/jitv.v10i2.469

Abstract

Aflatoxins are toxic compounds which occurred in cereals especially low qualities corn and peanuts. Aflatoxins are mutagenic, teratogenic and carcinogenic. The presence of aflatoxin in food including derived food in Indonesia had been observed, however, the observation on its toxicity effect is still limited. This research was conducted to study the effect of innoculation of aflatoxin B1 (AFB1) on the development of embryonic chicken egg, mortality and hatchability. The AFB1 was innoculated 10 μl in each 5 days age embryonic egg through air sacs dosaged 0; 15,6; 31,2; 62,5; 125 and 250 ng. The results showed that hatchability of those embryos were 66, 28, 26, 16, 0 and 0% respectively for 0; 15,6; 31,2; 62,5; 125 and 250 ng innoculation of AFB1. Innoculation of AFB1 caused malformation of the embryos, malabsorbtion of the yolk egg. The weight of hatched eggs was not significantly different in each group, eventhough there was a tendency that high AFB1 innoculation will decreased the live weight.     Key Words: Aflatoxin B1, Toxicity, Chick Embryo
Trenbolone residue in tissues and urine of Onggole male calves treated with acetate trenbolone implant Widiastuti, R; Firmansyah, R; ., Indraningsih
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.159 KB) | DOI: 10.14334/jitv.v12i1.565

Abstract

Trenbolone acetate (TBA) is a hormone being permitted to be used as growth promoters for livestocks in several meat-exporting countries. The presence of trenbolone residu in animal products might affect human health. The purpose of this study was to determine the distribution of trenbolone residue (TBA dan 17 ß-trenbolone) in tissues and urine of Onggole male calves. The implantation of 200 mg TBA as Finaplix-H® was done subcutaneously on the back side of the medial part of ear. Urine were collected periodically until 21 days post implantation. The animals were terminated on the day 21st post implantation. Urine, meat and organs were analysed for trenbolone residues. The results showed that TBA residues were detected in tissues of inner and surrounding areas of the implantation sites and liver with an average concentration of 11 ng/g, 2,1 ng/g and 1,6 ng/g respectively. The 17 ß-trenbolone residue was only detected in tissue of inner area of the implantation site at the average concentration of 8,2 ng/g. Meanwhile, none of the residues were detected in urine. Key Words: Residue, Trenbolone, Implantation
Suplementasi Greentea Powder pada Cookies sebagai Upaya Penurunan Kadar Zat Besi Darah Penderita Thalassemia Nuraeni, I; Proveravati, Atikah; Widiastuti, R
Jurnal Keperawatan Soedirman Vol. 09 No. 3 (2014)
Publisher : Jurusan Keperawatan FIKES UNSOED

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jks.2014.9.3.607

Abstract

Thalassemia  merupakan penyakit kelainan darah yang disebabkan oleh adanya kelainan pada gen globin alpha atau beta sehingga  produksi  rantai  globin  tersebut berkurang atau tidak ada. Penelitian ini secara umum bertujuan untuk membuat suatu produk makanan fungsional bagi penderita thalassemia yang berfungsi untuk menstabilkan kadar zat besi dalam tubuh penderita yang menjalani transfusi darah. Jenis penelitian ini termasuk penelitian eksperimental, dengan menggunakan desain Rancangan Acak Kelompok (RAK), Teknik analisis data dilakukan dengan uji anova. Untuk mengetahui pengaruh perlakuan yang diberikan pada tiap unit percobaan atau sampel terhadap parameter fisikokimia, maka dilakukan uji pembeda dengan Uji Duncan (DMRT) pada taraf 5%. Penelitian dilakukan dilakukan dengan mensuplementasikan green tea powder pada konsentrasi 25%, 35%, 45%, 55% dan 65% pada cookies dan tiap-tiap perlakuan dilakukan 4 kali ulangan. Hasil penelitian menunjukkan bahwa dengan suplementasi green tea powder pada konsentrasi 25%, 35%, 45%, 55% dan 65% tidak memberikan pengaruh yang nyata pada kadar air, kadar abu, kadar protein, kadar lemak, kadar karbohidrat dan kadar serat kasar produk cookies, sedangkan memberikan pengaruh yang nyata pada kadar tanin green tea cookies. Untuk uji organoleptik suplementasi greentea powder tiap-tiap perlakuan memberikan pengaruh yang signifikan pada tingkat kesukaan, warna, rasa dan aroma produk cookies, namun tidak memberikan pengaruh yang signifikan pada tekstur cookies. 
The residue of trenbolone from male Garut sheep which implanted by trenbolone acetate R Widiastuti; Indraningsih .; T.B Murdiarti; R Firmansyah
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 3 (2001): SEPTEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.817 KB) | DOI: 10.14334/jitv.v6i3.233

Abstract

Trenbolone acetate (TBA) is a growth hormone promoter which is implanted into animal to increase the body weight. The implantation of TBA in animal may cause the occurrence of residues of TBA and its metabolite (17-β-trenbolone). The presence of the residue might threat to human health. The aim of this research work was to study the presence of the residue of trenbolone in male Garut sheep which is implanted by TBA. The sheep were divided into 2 groups, those were D1 which were implanted by 40 mg TBA and D2 which were implanted by 60 mg TBA. One animal each from D1 and D2 were killed in the week 1, 2, 3 and 4 after implantation. And 24 hours before slaughtering the urine of D1 and D2 were collected using the metabolism cages. The samples for residue analysis were the organs such as liver, kidney, and the muscles around the implantation sites i.e inner and outer, upper front leg and upper back leg. The samples were extracted by organic solvents and analysed by HPLC. The residue of trenbolone occured in all samples of D1 and D2 which killed on 1 and 2 weeks after implantation. The residues occured on those which were killed 3 weeks after implantation were only in inner and outer samples. The residues in meat and organs were not detected in D1 nor D2 which were killed 4 weeks after implantation. The residue of trenbolone was also detected in the urine and still were detected in D1 and D2 which implanted by TBA for 4 weeks.   Key words : trenbolone acetate, 17-β-trenbolone, residue, sheep
The effect of aflatoxins B1 (AFB1) on chick embryo Sjamsul Bahri; R Widiastuti; Y Mustikaningsih
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 2 (2005): JUNE 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (298.84 KB) | DOI: 10.14334/jitv.v10i2.469

Abstract

Aflatoxins are toxic compounds which occurred in cereals especially low qualities corn and peanuts. Aflatoxins are mutagenic, teratogenic and carcinogenic. The presence of aflatoxin in food including derived food in Indonesia had been observed, however, the observation on its toxicity effect is still limited. This research was conducted to study the effect of innoculation of aflatoxin B1 (AFB1) on the development of embryonic chicken egg, mortality and hatchability. The AFB1 was innoculated 10 μl in each 5 days age embryonic egg through air sacs dosaged 0; 15,6; 31,2; 62,5; 125 and 250 ng. The results showed that hatchability of those embryos were 66, 28, 26, 16, 0 and 0% respectively for 0; 15,6; 31,2; 62,5; 125 and 250 ng innoculation of AFB1. Innoculation of AFB1 caused malformation of the embryos, malabsorbtion of the yolk egg. The weight of hatched eggs was not significantly different in each group, eventhough there was a tendency that high AFB1 innoculation will decreased the live weight.     Key Words: Aflatoxin B1, Toxicity, Chick Embryo
Trenbolone residue in tissues and urine of Onggole male calves treated with acetate trenbolone implant R Widiastuti; R Firmansyah; Indraningsih .
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.159 KB) | DOI: 10.14334/jitv.v12i1.565

Abstract

Trenbolone acetate (TBA) is a hormone being permitted to be used as growth promoters for livestocks in several meat-exporting countries. The presence of trenbolone residu in animal products might affect human health. The purpose of this study was to determine the distribution of trenbolone residue (TBA dan 17 ß-trenbolone) in tissues and urine of Onggole male calves. The implantation of 200 mg TBA as Finaplix-H® was done subcutaneously on the back side of the medial part of ear. Urine were collected periodically until 21 days post implantation. The animals were terminated on the day 21st post implantation. Urine, meat and organs were analysed for trenbolone residues. The results showed that TBA residues were detected in tissues of inner and surrounding areas of the implantation sites and liver with an average concentration of 11 ng/g, 2,1 ng/g and 1,6 ng/g respectively. The 17 ß-trenbolone residue was only detected in tissue of inner area of the implantation site at the average concentration of 8,2 ng/g. Meanwhile, none of the residues were detected in urine. Key Words: Residue, Trenbolone, Implantation
Aflatoxin contamination in feeds and feed ingredients from Lampung and East Java provinces Sjamsul Bahri; R Maryam; R Widiastuti
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 3 (2005): SEPTEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.67 KB) | DOI: 10.14334/jitv.v10i3.448

Abstract

In the last few years, poultry industries (broiler and laying hens) in Indonesia are faced on several problems, vaccination for instance, which suspected relate to highly-contaminated aflatoxins in feeds. The aim of this study is to give recent information on aflatoxin contamination in feeds, feed ingredients, and foods originated from some districts in Lampung and East Java provinces. The results of the study indicated that the percentage of aflatoxin B1 contamination in corn, commercial feeds, and peanuts in Lampung were 86.7, 70.0 and 80.0% with the average levels of 31.5, 13.5 and 32.2 μg/kg, respectively. Low levels of the other aflatoxins (AFB2, AFG1, and AFG2) were also detected in the samples. The highest percentage of aflatoxin contamination was found in samples from retailer (100%), followed by samples from traditional markets (91.7%), poultry shops (76.9), and a feed company (33.3%). The average levels of aflatoxin found were 58.8, 34.3, 17.8 and 2.9 μg/kg, respectively. In East Java, AFB1 was detected in all samples (corn, rice bran, concentrate and commercial feeds) on the average levels of 25.4, 69.7, 134.2 and 30.7 μg/kg respectively. The levels of AFG1 on the samples from East Java were much more higher than those of from Lampung, as well as the percentage.     Key Words: Aflatoxins, Feed, Feed Ingredients, Lampung, East Java