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The residue of trenbolone from male Garut sheep which implanted by trenbolone acetate Widiastuti, R; ., Indraningsih; Murdiarti, T.B; Firmansyah, R
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 3 (2001)
Publisher : Indonesian Animal Sciences Society

Trenbolone acetate (TBA) is a growth hormone promoter which is implanted into animal to increase the body weight. The implantation of TBA in animal may cause the occurrence of residues of TBA and its metabolite (17-Î²-trenbolone). The presence of the residue might threat to human health. The aim of this research work was to study the presence of the residue of trenbolone in male Garut sheep which is implanted by TBA. The sheep were divided into 2 groups, those were D1 which were implanted by 40 mg TBA and D2 which were implanted by 60 mg TBA. One animal each from D1 and D2 were killed in the week 1, 2, 3 and 4 after implantation. And 24 hours before slaughtering the urine of D1 and D2 were collected using the metabolism cages. The samples for residue analysis were the organs such as liver, kidney, and the muscles around the implantation sites i.e inner and outer, upper front leg and upper back leg. The samples were extracted by organic solvents and analysed by HPLC. The residue of trenbolone occured in all samples of D1 and D2 which killed on 1 and 2 weeks after implantation. The residues occured on those which were killed 3 weeks after implantation were only in inner and outer samples. The residues in meat and organs were not detected in D1 nor D2 which were killed 4 weeks after implantation. The residue of trenbolone was also detected in the urine and still were detected in D1 and D2 which implanted by TBA for 4 weeks. Â Key words : trenbolone acetate, 17-Î²-trenbolone, residue, sheep

Aflatoxin contamination in feeds and feed ingredients from Lampung and East Java provinces Bahri, Sjamsul; Maryam, R; Widiastuti, R
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 3 (2005)
Publisher : Indonesian Animal Sciences Society

In the last few years, poultry industries (broiler and laying hens) in Indonesia are faced on several problems, vaccination for instance, which suspected relate to highly-contaminated aflatoxins in feeds. The aim of this study is to give recent information on aflatoxin contamination in feeds, feed ingredients, and foods originated from some districts in Lampung and East Java provinces. The results of the study indicated that the percentage of aflatoxin B1 contamination in corn, commercial feeds, and peanuts in Lampung were 86.7, 70.0 and 80.0% with the average levels of 31.5, 13.5 and 32.2 Î¼g/kg, respectively. Low levels of the other aflatoxins (AFB2, AFG1, and AFG2) were also detected in the samples. The highest percentage of aflatoxin contamination was found in samples from retailer (100%), followed by samples from traditional markets (91.7%), poultry shops (76.9), and a feed company (33.3%). The average levels of aflatoxin found were 58.8, 34.3, 17.8 and 2.9 Î¼g/kg, respectively. In East Java, AFB1 was detected in all samples (corn, rice bran, concentrate and commercial feeds) on the average levels of 25.4, 69.7, 134.2 and 30.7 Î¼g/kg respectively. The levels of AFG1 on the samples from East Java were much more higher than those of from Lampung, as well as the percentage. Â Â Key Words: Aflatoxins, Feed, Feed Ingredients, Lampung, East Java

The effect of aflatoxins B1 (AFB1) on chick embryo Bahri, Sjamsul; Widiastuti, R; Mustikaningsih, Y
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 2 (2005)
Publisher : Indonesian Animal Sciences Society

Aflatoxins are toxic compounds which occurred in cereals especially low qualities corn and peanuts. Aflatoxins are mutagenic, teratogenic and carcinogenic. The presence of aflatoxin in food including derived food in Indonesia had been observed, however, the observation on its toxicity effect is still limited. This research was conducted to study the effect of innoculation of aflatoxin B1 (AFB1) on the development of embryonic chicken egg, mortality and hatchability. The AFB1 was innoculated 10 Î¼l in each 5 days age embryonic egg through air sacs dosaged 0; 15,6; 31,2; 62,5; 125 and 250 ng. The results showed that hatchability of those embryos were 66, 28, 26, 16, 0 and 0% respectively for 0; 15,6; 31,2; 62,5; 125 and 250 ng innoculation of AFB1. Innoculation of AFB1 caused malformation of the embryos, malabsorbtion of the yolk egg. The weight of hatched eggs was not significantly different in each group, eventhough there was a tendency that high AFB1 innoculation will decreased the live weight. Â Â Key Words: Aflatoxin B1, Toxicity, Chick Embryo

Trenbolone residue in tissues and urine of Onggole male calves treated with acetate trenbolone implant Widiastuti, R; Firmansyah, R; ., Indraningsih
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

Trenbolone acetate (TBA) is a hormone being permitted to be used as growth promoters for livestocks in several meat-exporting countries. The presence of trenbolone residu in animal products might affect human health. The purpose of this study was to determine the distribution of trenbolone residue (TBA dan 17 Ã-trenbolone) in tissues and urine of Onggole male calves. The implantation of 200 mg TBA as Finaplix-HÂ® was done subcutaneously on the back side of the medial part of ear. Urine were collected periodically until 21 days post implantation. The animals were terminated on the day 21st post implantation. Urine, meat and organs were analysed for trenbolone residues. The results showed that TBA residues were detected in tissues of inner and surrounding areas of the implantation sites and liver with an average concentration of 11 ng/g, 2,1 ng/g and 1,6 ng/g respectively. The 17 Ã-trenbolone residue was only detected in tissue of inner area of the implantation site at the average concentration of 8,2 ng/g. Meanwhile, none of the residues were detected in urine. Key Words: Residue, Trenbolone, Implantation

The residue of trenbolone from male Garut sheep which implanted by trenbolone acetate R Widiastuti; Indraningsih .; T.B Murdiarti; R Firmansyah
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 3 (2001): SEPTEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Trenbolone acetate (TBA) is a growth hormone promoter which is implanted into animal to increase the body weight. The implantation of TBA in animal may cause the occurrence of residues of TBA and its metabolite (17-β-trenbolone). The presence of the residue might threat to human health. The aim of this research work was to study the presence of the residue of trenbolone in male Garut sheep which is implanted by TBA. The sheep were divided into 2 groups, those were D1 which were implanted by 40 mg TBA and D2 which were implanted by 60 mg TBA. One animal each from D1 and D2 were killed in the week 1, 2, 3 and 4 after implantation. And 24 hours before slaughtering the urine of D1 and D2 were collected using the metabolism cages. The samples for residue analysis were the organs such as liver, kidney, and the muscles around the implantation sites i.e inner and outer, upper front leg and upper back leg. The samples were extracted by organic solvents and analysed by HPLC. The residue of trenbolone occured in all samples of D1 and D2 which killed on 1 and 2 weeks after implantation. The residues occured on those which were killed 3 weeks after implantation were only in inner and outer samples. The residues in meat and organs were not detected in D1 nor D2 which were killed 4 weeks after implantation. The residue of trenbolone was also detected in the urine and still were detected in D1 and D2 which implanted by TBA for 4 weeks. Key words : trenbolone acetate, 17-β-trenbolone, residue, sheep

The effect of aflatoxins B1 (AFB1) on chick embryo Sjamsul Bahri; R Widiastuti; Y Mustikaningsih
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 2 (2005): JUNE 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Aflatoxins are toxic compounds which occurred in cereals especially low qualities corn and peanuts. Aflatoxins are mutagenic, teratogenic and carcinogenic. The presence of aflatoxin in food including derived food in Indonesia had been observed, however, the observation on its toxicity effect is still limited. This research was conducted to study the effect of innoculation of aflatoxin B1 (AFB1) on the development of embryonic chicken egg, mortality and hatchability. The AFB1 was innoculated 10 μl in each 5 days age embryonic egg through air sacs dosaged 0; 15,6; 31,2; 62,5; 125 and 250 ng. The results showed that hatchability of those embryos were 66, 28, 26, 16, 0 and 0% respectively for 0; 15,6; 31,2; 62,5; 125 and 250 ng innoculation of AFB1. Innoculation of AFB1 caused malformation of the embryos, malabsorbtion of the yolk egg. The weight of hatched eggs was not significantly different in each group, eventhough there was a tendency that high AFB1 innoculation will decreased the live weight. Key Words: Aflatoxin B1, Toxicity, Chick Embryo

Trenbolone residue in tissues and urine of Onggole male calves treated with acetate trenbolone implant R Widiastuti; R Firmansyah; Indraningsih .
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Trenbolone acetate (TBA) is a hormone being permitted to be used as growth promoters for livestocks in several meat-exporting countries. The presence of trenbolone residu in animal products might affect human health. The purpose of this study was to determine the distribution of trenbolone residue (TBA dan 17 ß-trenbolone) in tissues and urine of Onggole male calves. The implantation of 200 mg TBA as Finaplix-H® was done subcutaneously on the back side of the medial part of ear. Urine were collected periodically until 21 days post implantation. The animals were terminated on the day 21st post implantation. Urine, meat and organs were analysed for trenbolone residues. The results showed that TBA residues were detected in tissues of inner and surrounding areas of the implantation sites and liver with an average concentration of 11 ng/g, 2,1 ng/g and 1,6 ng/g respectively. The 17 ß-trenbolone residue was only detected in tissue of inner area of the implantation site at the average concentration of 8,2 ng/g. Meanwhile, none of the residues were detected in urine. Key Words: Residue, Trenbolone, Implantation

Aflatoxin contamination in feeds and feed ingredients from Lampung and East Java provinces Sjamsul Bahri; R Maryam; R Widiastuti
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 3 (2005): SEPTEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

In the last few years, poultry industries (broiler and laying hens) in Indonesia are faced on several problems, vaccination for instance, which suspected relate to highly-contaminated aflatoxins in feeds. The aim of this study is to give recent information on aflatoxin contamination in feeds, feed ingredients, and foods originated from some districts in Lampung and East Java provinces. The results of the study indicated that the percentage of aflatoxin B1 contamination in corn, commercial feeds, and peanuts in Lampung were 86.7, 70.0 and 80.0% with the average levels of 31.5, 13.5 and 32.2 μg/kg, respectively. Low levels of the other aflatoxins (AFB2, AFG1, and AFG2) were also detected in the samples. The highest percentage of aflatoxin contamination was found in samples from retailer (100%), followed by samples from traditional markets (91.7%), poultry shops (76.9), and a feed company (33.3%). The average levels of aflatoxin found were 58.8, 34.3, 17.8 and 2.9 μg/kg, respectively. In East Java, AFB1 was detected in all samples (corn, rice bran, concentrate and commercial feeds) on the average levels of 25.4, 69.7, 134.2 and 30.7 μg/kg respectively. The levels of AFG1 on the samples from East Java were much more higher than those of from Lampung, as well as the percentage. Key Words: Aflatoxins, Feed, Feed Ingredients, Lampung, East Java

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