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The validity of rapid test to detect prostate-specific antigen (PSA) in seminal fluid Henky, Henky; Budiningsih, Yuli; Widiatmaka, Wibisana
Medical Journal of Indonesia Vol 20, No 4 (2011): November
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1001.846 KB) | DOI: 10.13181/mji.v20i4.464

Abstract

Background: This study was conducted to determine whether the rapid test device can be used to detect PSA in seminal fluid specifically, for solving sexual assault cases.Methods: A cross sectional study has been conducted. A total of 45 samples were taken consecutively. Semen was diluted in serially up to 1:5.000.000 and male urin up to 1:200 using distilled water, whereas female urin was not diluted. Samples were analyzed using rapid test PSA.Results: The proportion of positive results of PSA in seminal fluid, male urin and female urin respectively was 100%, 6.67%, and 0%. Statistically, these differences are highly significant. The analysis revealed that the PSA rapid test device was 100% sensitive and 96.67% specific to detect seminal fluid. The test also have PPV 93.75%, NPV 100%, LR(+) 33.33, LR(-) 0, and AUC 0.983.Conclusion: PSA Rapid Test is very specific and sensitive to detect PSA in seminal fluid. Therefore, this device is suggested for forensic use in sexual assault cases. (Med J Indones 2011; 20:278-82)Keywords: PSA, rapid test, sexual assault
Penentuan Umur Bercak Darah Manusia pada Kain Katun dengan Menggunakan High Performance Liquid Chromatography Mohammad Tegar Indrayana; Wibisana Widiatmaka; Ade Firmansyah Sugiharto; Ridla Bakri
Jurnal Ilmu Kedokteran Vol 8, No 1 (2014): Jurnal Ilmu Kedokteran
Publisher : Fakultas Kedokteran Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.923 KB) | DOI: 10.26891/JIK.v8i1.2014.8-17

Abstract

This research is to study the method in determining the age of human bloodstain due the rarity of human bloodstainresearches in Indonesia, by analyzing the X and Y peaks that appeared in High Performance Liquid Chromatography(HPLC). This research is experimental with time series for 11 days observation by exposing the bloodstain onto 33pieces of cotton fabric, each measuring 1 cm x 1 cm that originated from 3 respondents with environmental conditionsof the FKUI/RSUPN Dr. Cipto Mangunkusumo Forensic and Medico-legal Department which represents the area ofCentral Jakarta. The results of this research is the X and Y peaks do not point to a linearity so that the criteria for linearregression equation is not met. The conclusion of this research is age of human bloodstain on cotton fabric byanalyzing X peak with HPLC cannot yet be used pragmatically in the field in accord to the conditions at the crimescene.