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All Journal Biology, Medicine, & Natural Product Chemistry Biosaintifika: Journal of Biology & Biology Education SEMAR Bioscientist : Jurnal Ilmiah Biologi Prosiding Konferensi Nasional PKM-CSR
TETRI WIDIYANI
Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Jalan Ir. Sutami 36 A, Surakarta 57126, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences

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Ascaris suum Cuticle Ultrastructure Due to the In Vitro Application of Ethanol Extract to Fruits Musa x paradisiaca L. ‘Pisang Ambon’ Haryatmi, Dwi; Astirin, Okid Parama; Widiyani, Tetri
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11918

Abstract

Species of Musa have various activities, one of which is anthelmintic activity. Part of banana plants that can be used as anthelmintic is part of the fruit, because of the phytochemical content possessed. Tannin is able to inhibit the enzyme cholinesterase, damage the membrane of the worm and can precipitate the protein.  The purpose of this research is to know the content of tannin and also total tannin level on ethanol extract of Musa x paradisiaca L. ‘Pisang Ambon’ and in vitro effect of the extract on Ascaris suum cuticle.  Thin Layer Chromatography (TLC) showed of ethanol extract of Ambon banana containing tannin with a total test of tannin was 54.98% w/w. In vitro at negative control (NaCl 0.9%) worm death occurred at 289 hours, positive control (pyrantel pamoate 5 mg/ml) at 1 hour, extract concentration 200 mg/ml at hour 27 and concentration 400 mg/ml at 1 hour. Histopathological examination and scanning electron microscope showed a degenerative change in ultrastructures of the worms cuticle. From the results of this study, it can be concluded that exposure of ethanol extract to Ambon banana give a damaging effect to cuticle ultrastructure in the form of crack/break, perforation and separation of muscular from cuticle and edema on A. suum worm. High tannin content in the raw/green ambon banana, can be developed to eradicate ascariasis including egg stage which until now cannot be done with synthetic drugs due to the thickness and strength of layers of worm eggs consisting of layers of protein and lipids.
High Performance Liquid Chromatography (HPLC) for Detection of Glucosamine and Chondroitin Sulfate Compounds Rakhmiyati, Rakhmiyati; Widiyani, Tetri; Budiharjo, Agung
Biology, Medicine, & Natural Product Chemistry Vol 12, No 1 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.121.5-8

Abstract

Glucosamine and chondroitin sulfate are compounds found in shark cartilage (Carcharhinus sorrah). The two compounds have many health benefits, that is wound healing and helping the process of angiogenesis. This study aims to determine the content of glucosamine and chondroitin sulfate compounds in shark cartilage (SC) extract. The method used was High Performance Liquid Chromatography (HPLC) with potassium phosphate buffer solution at pH 3. The results of this research were SC extract contained glucosamine and chondroitin sulfate compounds with a retention time of 1.914 minutes.
KOLABORASI PRODI BIOLOGI FMIPA UNS DALAM PERKUATAN KOMPETENSI IPA BAGI SISWA SMP DJAMA’ATUL ICHWAN, SURAKARTA Avi, Salsabilla El; Nurrahmah, Alfhiya; Herawati, Elisa; Listyawati, Shanti; Widiyani, Tetri
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 7 (2024): PKMCSR2024: Kolaborasi Hexahelix dalam Optimalisasi Potensi Pariwisata di Indonesia: A
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37695/pkmcsr.v7i0.2380

Abstract

Praktikum menjadi salah satu sarana dalam peningkatan kompetensi IPA bagi siswa di sekolah. Implementasi dari praktikum dapat memberikan dampak positif dalam peningkatan kompetensi karena pembelajaran akan lebih berkesan dan mudah dipahami jika dilaksanakan dengan aksi yang dikerjakan langsung oleh siswa. Hal tersebut yang mendasari sekolah, baik di tingkat SMP maupun SMA, melakukan banyak praktikum untuk meningkatkan kompetensi IPA yang dimiliki muridnya. Berdasarkan dari hasil survei yang telah dilakukan, banyak implementasi praktikum yang selinier dengan pembelajaran IPA di jenjang SMP sehingga program kerja yang dilakukan berfokus pada kegiatan praktikum yang dapat menunjang peningkatan kompetensi siswa di SMP Djama’atul Ichwan, Surakarta. Program-program tersebut terdiri dari 5 program kerja yang satu di antaranya merupakan program kerja sama dengan dosen Grup Riset Biomaterial Hewan Prodi Biologi FMIPA UNS berupa pendampingan praktik mikroskopis. Kelima program kerja tersebut di antaranya, sosialisasi pengelolaan limbah organik melalui praktikum pembuatan eco-enzyme; praktikum pembuatan insektarium dalam upaya meningkatkan pembelajaran klasifikasi dan sistematika hewan; praktikum pengamatan morfologi dan anatomi hewan uji laboratorium; praktikum pembuatan preparat sederhana sebagai objek pengamatan dengan mikroskop; serta kegiatan mini project yang menekankan skill kerja sama dan kepemimpinan antarsiswa dengan mengimplementasikan pembelajaran IPA yang telah didapat.
Efektivitas Antidiabetes Ekstrak Bribil (Galinsoga parviflora) pada Tikus (Rattus norvegicus) Model Diabetes Melitus Tipe 2 Samosir, Monicha Desry; Listyawati, Shanti; Widiyani, Tetri
Bioscientist : Jurnal Ilmiah Biologi Vol 13, No 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.15219

Abstract

This study aims to examine the effectiveness of G. parviflora extract in reducing fasting blood glucose (FBS) levels and improving lipid profiles. The research design used was a laboratory experiment with a Completely Randomized Design (CRD). This study was conducted from June 2024 to January 2025 in the Laboratory of Biology Study Program and Integrated Mathematics and Natural Sciences Laboratory. Rats were divided into five groups, namely negative control, positive control (glibenclamide 5 mg/kgBB), and three treatment groups of bribil extract with doses of 400 mg/kgBB, 600 mg/kgBB, and 800 mg/kgBB. Parameters measured included GDP levels and lipid profiles (total cholesterol, triglyceride, HDL, and LDL levels). Data analysis was performed using one-way ANOVA test and Tukey's Post Hoc further test. The results showed that the ethanol extract of bribil (Galinsoga parviflora) doses of 600 and 800 mg/kgBB effectively reduced GDP levels, total cholesterol levels, triglyceride levels, LDL levels, and HDL levels of rats (Rattus norvegicus) T2DM model. These results indicate that bribil extract has potential as a therapeutic agent for T2DM. These findings suggested the possibility of Galinsoga parviflora extract as a DMT2 therapeutic agent.
Efektivitas Antidiabetes Ekstrak Bribil (Galinsoga parviflora) pada Tikus (Rattus norvegicus) Model Diabetes Melitus Tipe 2 Samosir, Monicha Desry; Listyawati, Shanti; Widiyani, Tetri
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.15219

Abstract

This study aims to examine the effectiveness of G. parviflora extract in reducing fasting blood glucose (FBS) levels and improving lipid profiles. The research design used was a laboratory experiment with a Completely Randomized Design (CRD). This study was conducted from June 2024 to January 2025 in the Laboratory of Biology Study Program and Integrated Mathematics and Natural Sciences Laboratory. Rats were divided into five groups, namely negative control, positive control (glibenclamide 5 mg/kgBB), and three treatment groups of bribil extract with doses of 400 mg/kgBB, 600 mg/kgBB, and 800 mg/kgBB. Parameters measured included GDP levels and lipid profiles (total cholesterol, triglyceride, HDL, and LDL levels). Data analysis was performed using one-way ANOVA test and Tukey's Post Hoc further test. The results showed that the ethanol extract of bribil (Galinsoga parviflora) doses of 600 and 800 mg/kgBB effectively reduced GDP levels, total cholesterol levels, triglyceride levels, LDL levels, and HDL levels of rats (Rattus norvegicus) T2DM model. These results indicate that bribil extract has potential as a therapeutic agent for T2DM. These findings suggested the possibility of Galinsoga parviflora extract as a DMT2 therapeutic agent.
Pendampingan dan peningkatan kompetensi praktek biologi mikroskopis bagi siswa SMP Djama’atul Ichwan, Surakarta Herawati, Elisa; Listyawati, Shanti; Widiyani, Tetri; Budiharjo, Agung; Astirin, Okid Parama
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 13, No 2 (2024): November
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v13i2.87836

Abstract

Biologi mikroskopis mempelajari organisme pada level seluler dan menjadi bagian tak terpisahkan dari pengetahuan IPA modern. Di SMP Djama’atul Ichwan Surakarta, keterbatasan fasilitas membuat pembelajaran konsep-konsep biologi mikroskopis sulit dibarengi dengan pengalaman praktek di laboratorium sekolah. Mitra mengharapkan ada pendampingan untuk meningkatkan kompetensi praktek pada mata pelajaran biologi. Kegiatan pengabdian ini bertujuan meningkatkan kompetensi praktek dan wawasan umum siswa mengenai biologi mikroskopis yang tercakup dalam materi IPA di SMP. Kompetensi praktek biologi mikroskopis yang belum maksimal pada mitra berakar dari tidak adanya laboratorium IPA, media praktikum, dan mikroskop. Tim PKM UNS menghadirkan solusi berupa program pendampingan kepada siswa selama satu semester yaitu, 1) pengenalan mikroskop dan aplikasinya; 2) praktek membuat preparat mikroskopis sederhana sesuai materi SMP; 3) pengadaan mikroskop cahaya; modul praktikum; foldscope untuk mitra; 4) komunikasi selama semester berjalan dan umpan balik mengenai aktivitas praktikum dengan mikroskop di SMP. Keberhasilan kegiatan dapat dilihat dari indikator skor pre-/post-test, aktivitas praktikum, dan pengadaan mikroskop untuk SMP. Siswa menjadi paham prinsip kerja dan aplikasi mikroskop untuk visualisasi struktur seluler dan jaringan, hal ini tampak dari peningkatan skor post-test (>25% kenaikan skor) pada 72% siswa. Pada sesi praktikum, siswa mampu membuat dua preparat berbeda untuk diamati dengan mikroskop dan diidentifikasi bagian-bagiannya. Selanjutnya, siswa dapat melakukan praktek mandiri di SMP menggunakan mikroskop dari investasi kegiatan pengabdian ini Kata kunci: kompetensi praktik, biologi mikroskopis
Co-Authors Agung Budiharjo Avi, Salsabilla El Bambang Suryobroto Haryatmi, Dwi Herawati, Elisa Nurrahmah, Alfhiya Okid Parama Astirin Rakhmiyati, Rakhmiyati Samosir, Monicha Desry Shanti Listyawati Yuzuru Hamada