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Ascaris suum Cuticle Ultrastructure Due to the In Vitro Application of Ethanol Extract to Fruits Musa x paradisiaca L. ‘Pisang Ambon’ Haryatmi, Dwi; Astirin, Okid Parama; Widiyani, Tetri
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11918

Abstract

Species of Musa have various activities, one of which is anthelmintic activity. Part of banana plants that can be used as anthelmintic is part of the fruit, because of the phytochemical content possessed. Tannin is able to inhibit the enzyme cholinesterase, damage the membrane of the worm and can precipitate the protein.  The purpose of this research is to know the content of tannin and also total tannin level on ethanol extract of Musa x paradisiaca L. ‘Pisang Ambon’ and in vitro effect of the extract on Ascaris suum cuticle.  Thin Layer Chromatography (TLC) showed of ethanol extract of Ambon banana containing tannin with a total test of tannin was 54.98% w/w. In vitro at negative control (NaCl 0.9%) worm death occurred at 289 hours, positive control (pyrantel pamoate 5 mg/ml) at 1 hour, extract concentration 200 mg/ml at hour 27 and concentration 400 mg/ml at 1 hour. Histopathological examination and scanning electron microscope showed a degenerative change in ultrastructures of the worms cuticle. From the results of this study, it can be concluded that exposure of ethanol extract to Ambon banana give a damaging effect to cuticle ultrastructure in the form of crack/break, perforation and separation of muscular from cuticle and edema on A. suum worm. High tannin content in the raw/green ambon banana, can be developed to eradicate ascariasis including egg stage which until now cannot be done with synthetic drugs due to the thickness and strength of layers of worm eggs consisting of layers of protein and lipids.
KEPADATAN TULANG METACARPAL SIMPANSE USIA 0 SAMPAI 44 TAHUN Hamada, Yuzuru; Widiyani, Tetri; Suryobroto, Bambang
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.318 KB)

Abstract

ABSTRAK   Sifat fisik tulang merupakan indikator yang baik untuk studi pertumbuhan dan penuaan. Tulang adalah jaringan dinamis karena adanya proses modeling dan remodeling. Tulang berubah tidak hanya pada ukuran dan bentuknya, tetapi juga kepadatannya yang disebabkan  karena perubahan kandungan mineralnya. Osteoporosis atau kekeroposan tulang merupakan salah satu tanda umum penuaaan manusia. Osteoporosis di kalangan anthropoid masih belum diketahui. Dalam penelitian ini kami melakukan pengukuran kepadatan korteks tulang metacarpal simpanse (Pan troglodytes) usia 0 sampai 44 tahun berdasarkan radiografi. Pengukuran dilakukan dengan metode mikro-densitometri pada 68 simpanse betina dan 49 simpanse jantan. Kami menemukan bahwa kepadatan tulang meningkat pesat sampai usia sekitar 10 tahun. Pada simpanse jantan kepadatan tulangnya terus meningkat sampai usia 44 tahun, sedangkan pada simpanse betina kepadatan tulangnya menurun mulai usia 20 tahun. Penurunan kepadatan tulang simpanse betina dapat disebabkan karena kalsium tulang digunakan pada masa kehamilan dan menyusui. Namun demikian, simpanse betina diketahui tidak mengalami menopause. Jadi tidak seperti wanita, kejadian osteoporosis pada simpanse betina bukanlah akibat dari menopause. Kemungkinan hal ini berkaitan dengan berkurangnya kadar estrogen pada simpanse lanjut usia.   Kata Kunci: osteoporosis, kepadatan tulang, metakarpal, simpanse
High Performance Liquid Chromatography (HPLC) for Detection of Glucosamine and Chondroitin Sulfate Compounds Rakhmiyati, Rakhmiyati; Widiyani, Tetri; Budiharjo, Agung
Biology, Medicine, & Natural Product Chemistry Vol 12, No 1 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.121.5-8

Abstract

Glucosamine and chondroitin sulfate are compounds found in shark cartilage (Carcharhinus sorrah). The two compounds have many health benefits, that is wound healing and helping the process of angiogenesis. This study aims to determine the content of glucosamine and chondroitin sulfate compounds in shark cartilage (SC) extract. The method used was High Performance Liquid Chromatography (HPLC) with potassium phosphate buffer solution at pH 3. The results of this research were SC extract contained glucosamine and chondroitin sulfate compounds with a retention time of 1.914 minutes.
KOLABORASI PRODI BIOLOGI FMIPA UNS DALAM PERKUATAN KOMPETENSI IPA BAGI SISWA SMP DJAMA’ATUL ICHWAN, SURAKARTA Avi, Salsabilla El; Nurrahmah, Alfhiya; Herawati, Elisa; Listyawati, Shanti; Widiyani, Tetri
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 7 (2024): PKMCSR2024: Kolaborasi Hexahelix dalam Optimalisasi Potensi Pariwisata di Indonesia: A
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37695/pkmcsr.v7i0.2380

Abstract

Praktikum menjadi salah satu sarana dalam peningkatan kompetensi IPA bagi siswa di sekolah. Implementasi dari praktikum dapat memberikan dampak positif dalam peningkatan kompetensi karena pembelajaran akan lebih berkesan dan mudah dipahami jika dilaksanakan dengan aksi yang dikerjakan langsung oleh siswa. Hal tersebut yang mendasari sekolah, baik di tingkat SMP maupun SMA, melakukan banyak praktikum untuk meningkatkan kompetensi IPA yang dimiliki muridnya. Berdasarkan dari hasil survei yang telah dilakukan, banyak implementasi praktikum yang selinier dengan pembelajaran IPA di jenjang SMP sehingga program kerja yang dilakukan berfokus pada kegiatan praktikum yang dapat menunjang peningkatan kompetensi siswa di SMP Djama’atul Ichwan, Surakarta. Program-program tersebut terdiri dari 5 program kerja yang satu di antaranya merupakan program kerja sama dengan dosen Grup Riset Biomaterial Hewan Prodi Biologi FMIPA UNS berupa pendampingan praktik mikroskopis. Kelima program kerja tersebut di antaranya, sosialisasi pengelolaan limbah organik melalui praktikum pembuatan eco-enzyme; praktikum pembuatan insektarium dalam upaya meningkatkan pembelajaran klasifikasi dan sistematika hewan; praktikum pengamatan morfologi dan anatomi hewan uji laboratorium; praktikum pembuatan preparat sederhana sebagai objek pengamatan dengan mikroskop; serta kegiatan mini project yang menekankan skill kerja sama dan kepemimpinan antarsiswa dengan mengimplementasikan pembelajaran IPA yang telah didapat.
Efektivitas Antidiabetes Ekstrak Bribil (Galinsoga parviflora) pada Tikus (Rattus norvegicus) Model Diabetes Melitus Tipe 2 Samosir, Monicha Desry; Listyawati, Shanti; Widiyani, Tetri
Bioscientist : Jurnal Ilmiah Biologi Vol 13, No 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.15219

Abstract

This study aims to examine the effectiveness of G. parviflora extract in reducing fasting blood glucose (FBS) levels and improving lipid profiles. The research design used was a laboratory experiment with a Completely Randomized Design (CRD). This study was conducted from June 2024 to January 2025 in the Laboratory of Biology Study Program and Integrated Mathematics and Natural Sciences Laboratory. Rats were divided into five groups, namely negative control, positive control (glibenclamide 5 mg/kgBB), and three treatment groups of bribil extract with doses of 400 mg/kgBB, 600 mg/kgBB, and 800 mg/kgBB. Parameters measured included GDP levels and lipid profiles (total cholesterol, triglyceride, HDL, and LDL levels). Data analysis was performed using one-way ANOVA test and Tukey's Post Hoc further test. The results showed that the ethanol extract of bribil (Galinsoga parviflora) doses of 600 and 800 mg/kgBB effectively reduced GDP levels, total cholesterol levels, triglyceride levels, LDL levels, and HDL levels of rats (Rattus norvegicus) T2DM model. These results indicate that bribil extract has potential as a therapeutic agent for T2DM. These findings suggested the possibility of Galinsoga parviflora extract as a DMT2 therapeutic agent.
Efektivitas Antidiabetes Ekstrak Bribil (Galinsoga parviflora) pada Tikus (Rattus norvegicus) Model Diabetes Melitus Tipe 2 Samosir, Monicha Desry; Listyawati, Shanti; Widiyani, Tetri
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.15219

Abstract

This study aims to examine the effectiveness of G. parviflora extract in reducing fasting blood glucose (FBS) levels and improving lipid profiles. The research design used was a laboratory experiment with a Completely Randomized Design (CRD). This study was conducted from June 2024 to January 2025 in the Laboratory of Biology Study Program and Integrated Mathematics and Natural Sciences Laboratory. Rats were divided into five groups, namely negative control, positive control (glibenclamide 5 mg/kgBB), and three treatment groups of bribil extract with doses of 400 mg/kgBB, 600 mg/kgBB, and 800 mg/kgBB. Parameters measured included GDP levels and lipid profiles (total cholesterol, triglyceride, HDL, and LDL levels). Data analysis was performed using one-way ANOVA test and Tukey's Post Hoc further test. The results showed that the ethanol extract of bribil (Galinsoga parviflora) doses of 600 and 800 mg/kgBB effectively reduced GDP levels, total cholesterol levels, triglyceride levels, LDL levels, and HDL levels of rats (Rattus norvegicus) T2DM model. These results indicate that bribil extract has potential as a therapeutic agent for T2DM. These findings suggested the possibility of Galinsoga parviflora extract as a DMT2 therapeutic agent.