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All Journal Jurnal Biologi Tropis Jurnal ABDINUS : Jurnal Pengabdian Nusantara Jurnal Kesehatan Republik Indonesia
Firman Saputra
Universitas Tanjungpura
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Veterinary

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Identification of Lactic Acid Bacteria From Pandan Civet Feces (P. hermaphroditus) in West Kalimantan Based on Phenotypic Similarity Ageresya Ester Evelin Br Sibarani; Rahmawati Rahmawati; Firman Saputra
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5314

Abstract

Lactic acid bacteria are included in the gram positive bacteria that are widely distributed in the digestive tract of living things, such as in the feces of the Pandan Luwak (P. hermaphroditus) and important role in improving the microbial balance in the body. The purpose of this study was to obtain phenotypic characters, types of bacteria and similarity relationship between lactic acid bacteria from feces of Pandan Civet (P. hermaphroditus) in West Kalimantan and lactic acid bacteria in the identification book Bergey's manual of determinative bacteriology. This research was conducted by the method of isolation and screening bacteria using MRSA media supplemented with CaCO3 1%. Characterization of lactic acid bacteria based on morphological, biochemical, and physiological characters in Bergey's Manual of Determination Bacteriology. The results of the analysis using the MVSP (Multivariant Statistic Package) program with SSM coefficients and the UPGMA method obtained 44 bacterial isolates  thought to belong to two genera, namely the genus Lactobacillus and Bifidobacterium with three species, namely L. casei, L. brevis and B. bifidum. The results of the analysis showed that 12 bacterial isolates had a similarity index of 87,6% to L. casei, 17 bacterial isolates had a similarity index of 92,2% to B. bifidum and 15 bacterial isolates had a similarity index of 84,5% to L. brevis.
The Diversity of Stingless Bees (Hymenoptera: Meliponini) in Belaban Resort, Bukit Baka Bukit Raya National Park Patrisia Lindawati; Firman Saputra; Kustiati; Didin Joharrudin
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6234

Abstract

Kelulut (Hymenoptera: Meliponini) is a honey-producing bee and belongs to the eusocial bee group. Kelulut is also a pollinating insect that is widespread in the world, including Indonesia. The study of kelulut diversity can describe the composition of kelulut in an area and the condition of an ecosystem. This study aimed to obtain data and information on kelulut diversity in three different forest areas (primary forest, secondary forest, and ecosystem restoration area). The study was conducted by capturing kelulut using sugar solution bait traps and free sampling. The results obtained were analyzed using the Shannon-Wiener Diversity Index (H'), the Evenness index (E) and the Simpson Dominance Index (C). A total of 747 individuals from 12 types of kelulut were found in this study. The secondary forest gets the highest H' value (1.869), followed by the primary forest (1.672) and ecosystem restoration area (1.159). The diversity of stingless bees is higher in forested areas due to environmental factors such as temperatures within the normal range (25.5-28.9°C), the availability of trees for nesting, and the abundance of food sources from flowers.
Efektivitas Ekstrak Etanol Bawang Putih (Allium Sativum L.) Sebagai Larvasida Alami Pada Larva Aedes Aegypti Yolanda Oktavianingsih; Sari Rahmayanti; Firman Saputra
Jurnal Kesehatan Republik Indonesia Vol 3 No 5 (2026): JKRI - April 2026
Publisher : PT. INOVASI TEKNOLOGI KOMPUTER

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Abstract

Penyakit Demam Berdarah Dengue (DBD) adalah penyakit menular karena infeksi virus dengue (DENV). Nyamuk Aedes aegypti yang terinfeksi virus dengue berperan sebagai vektor perantara. Penggunaan larvasida merupakan salah satu upaya pencegahan penyakit ini. Larvasida alami diharapkan dapat mengurangi dampak negatif larvasida sintetik. Bawang putih mengandung senyawa metabolit sekunder, seperti flavonoid, alkaloid, tanin, saponin dan organosulfur yang berpotensi sebagai larvasida. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak etanol bawang putih (Allium sativum L.) sebagai larvasida alami pada larva Aedes aegypti. Hewan uji yang digunakan adalah larva Aedes aegypti instar III. Kelompok uji berupa ekstrak etanol bawang putih (Allium sativum L.) dengan 5 dosis, yaitu 375 ppm, 500 ppm, 625 ppm, 750 ppm dan 1000 ppm, serta kontrol negatif berupa aquades 100 ml dan kontrol positif berupa temephos 0,02 ppm. Pengujian ini diulang sebanyak 3 kali dalam satu waktu selama 24 jam. Ekstrak etanol bawang putih mengandung senyawa metabolit sekunder berupa flavonoid, alkaloid, tanin, saponin dan organosulfur. Persentase mortalitas larva yang dihasilkan oleh ekstrak etanol bawang putih dosis 375 ppm, 500 ppm, 625 ppm, 750 ppm dan 1000 ppm secara berturut-turut adalah 14,67%, 22,67%, 37,33%, 53,33% dan 70,67%, sedangkan pada kontrol negatif dan positif masing-masing adalah 0% dan 100%. Uji Kruskal-Wallis menunjukkan bahwa terdapat perbedaan nilai rata-rata mortalitas yang signifikan antar kelompok uji (Sig.= 0,003). Nilai LC50 adalah 785,391 ppm (LC50>750 ppm) yang artinya aktivitas larvasida tergolong tidak aktif, sedangkan LC90 adalah 1452,280 ppm. Ekstrak etanol bawang putih (Allium sativum L.) memiliki aktivitas larvasida, tetapi dosis yang diuji belum efektif sebagai larvasida alami pada larva Aedes aegypti apabila dibandingkan dengan kontrol positif
Uji Efektivitas Ekstrak Kloroform Bawang Putih (Allium Sativum) Sebagai Larvasida Terhadap Larva Aedes Aegypti Candra Aurelion; Sari Rahmayanti; Firman Saputra
Jurnal Kesehatan Republik Indonesia Vol 3 No 8 (2026): JKRI - Juli 2026
Publisher : PT. INOVASI TEKNOLOGI KOMPUTER

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Abstract

Demam berdarah dengue (DBD) adalah penyakit arbovirus (arthropod-borne viral disease) yang disebabkan oleh virus dengue yang dibawa oleh vektor penyakit berupa nyamuk, yaitu nyamuk Aedes aegypti sebagai vektor utama dan nyamuk Aedes albopictus sebagai vektor sekunder. Pencegahan penyakit ini dapat dilakukan dengan pengendalian vektor, salah satunya secara kimiawi dengan menggunakan larvasida berupa temefos. Larvasida alami berbasis tanaman diharapkan dapat mengurangi efek samping penggunaan larvasida sintetik. Bawang putih mengandung senyawa metabolit sekunder, seperti alkaloid, flavonoid, saponin, terpenoid dan organosulfur yang berpotensi sebagai larvasida. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak kloroform bawang putih (Allium sativum) sebagai larvasida terhadap larva Aedes aegypti. Uji larvasida dilakukan dengan memaparkan ekstrak kloroform bawang putih (Allium sativum) dalam 5 dosis berbeda (125 ppm, 175 ppm, 250 ppm, 350 ppm dan 500 ppm) terhadap larva Aedes aegypti instar III selama 24 jam. Kontrol negatif berupa akuades dengan tween 80 3% dan kontrol positif berupa temefos 0,02 ppm. Pengujian diulang sebanyak 3 kali. Persentase mortalitas larva Aedes aegypti yang dihasilkan oleh ekstrak kloroform bawang putih (Allium sativum) pada dosis 125 ppm, 175 ppm, 250 ppm, 350 ppm dan 500 ppm secara berturut-turut adalah 25,33%, 33,32%, 44%, 52% dan 68%. Persentase kematian larva pada kontrol negatif adalah 0% dan pada kontrol positif adalah 100%. Terdapat perbedaan bermakna antara mortalitas larva yang ditimbulkan oleh berbagai kelompok uji dengan kelompok kontrol positif. Nilai LC50 dan LC90 ekstrak kloroform bawang putih (Allium sativum) yang diperoleh pada penelitian ini adalah 299,839 ppm dan 1510,673 ppm. Ekstrak kloroform bawang putih (Allium sativum) tergolong efektif sebagai larvasida (LC50 < 750 ppm). Namun, belum lebih efektif jika dibandingkan dengan temefos
Pelatihan Pengolahan Air Kelapa menjadi Nata de Coco di Desa Sungai Kakap, Kalimantan Barat Rahmawati Rahmawati; Siti Khotimah; Mukarlina Mukarlina; Zulfa Zakiah; Firman Saputra; Masnur Turnip; Aotza Lisandra
Jurnal ABDINUS : Jurnal Pengabdian Nusantara Vol 10 No 1 (2026): Volume 10 Nomor 1 Tahun 2026
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/ja.v10i1.23985

Abstract

Old coconut water is often discarded by the community as waste. This community service activity aimed to provide training on processing coconut water into nata de coco for the members of the Family Welfare Empowerment Group (PKK) in Sungai Kakap Village, Kubu Raya Regency, West Kalimantan. The implementation of this community service program consisted of several stages, including dissemination/socialization, hands-on practice, and discussion sessions. The activity began with the preparation of tools and materials at the Biology Laboratory, Faculty of Mathematics and Natural Sciences, Tanjungpura University (FMIPA UNTAN), followed by a field visit to the target village, participant gathering, socialization of nata de coco production materials, joint practice with workshop participants, and a discussion session. The nata de coco production training was successfully carried out at the Sungai Kakap Village Office, West Kalimantan. Based on the questionnaire results, the PKK members stated that the activity was beneficial, and many expressed their interest in applying the process and producing nata de coco for personal consumption as well as for potential commercialization.
Co-Authors Ageresya Ester Evelin Br Sibarani Aotza Lisandra Candra Aurelion Didin Joharrudin Kustiati Masnur Turnip Mukarlina -, Mukarlina Patrisia Lindawati Rahmawati Rahmawati Sari Rahmayanti Siti Khotimah Yolanda Oktavianingsih Zulfa Zakiah