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All Journal Jurnal Biologi Tropis Jurnal Kesehatan Republik Indonesia
Firman Saputra
Universitas Tanjungpura
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Veterinary

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Identification of Lactic Acid Bacteria From Pandan Civet Feces (P. hermaphroditus) in West Kalimantan Based on Phenotypic Similarity Ageresya Ester Evelin Br Sibarani; Rahmawati Rahmawati; Firman Saputra
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5314

Abstract

Lactic acid bacteria are included in the gram positive bacteria that are widely distributed in the digestive tract of living things, such as in the feces of the Pandan Luwak (P. hermaphroditus) and important role in improving the microbial balance in the body. The purpose of this study was to obtain phenotypic characters, types of bacteria and similarity relationship between lactic acid bacteria from feces of Pandan Civet (P. hermaphroditus) in West Kalimantan and lactic acid bacteria in the identification book Bergey's manual of determinative bacteriology. This research was conducted by the method of isolation and screening bacteria using MRSA media supplemented with CaCO3 1%. Characterization of lactic acid bacteria based on morphological, biochemical, and physiological characters in Bergey's Manual of Determination Bacteriology. The results of the analysis using the MVSP (Multivariant Statistic Package) program with SSM coefficients and the UPGMA method obtained 44 bacterial isolates  thought to belong to two genera, namely the genus Lactobacillus and Bifidobacterium with three species, namely L. casei, L. brevis and B. bifidum. The results of the analysis showed that 12 bacterial isolates had a similarity index of 87,6% to L. casei, 17 bacterial isolates had a similarity index of 92,2% to B. bifidum and 15 bacterial isolates had a similarity index of 84,5% to L. brevis.
The Diversity of Stingless Bees (Hymenoptera: Meliponini) in Belaban Resort, Bukit Baka Bukit Raya National Park Patrisia Lindawati; Firman Saputra; Kustiati; Didin Joharrudin
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6234

Abstract

Kelulut (Hymenoptera: Meliponini) is a honey-producing bee and belongs to the eusocial bee group. Kelulut is also a pollinating insect that is widespread in the world, including Indonesia. The study of kelulut diversity can describe the composition of kelulut in an area and the condition of an ecosystem. This study aimed to obtain data and information on kelulut diversity in three different forest areas (primary forest, secondary forest, and ecosystem restoration area). The study was conducted by capturing kelulut using sugar solution bait traps and free sampling. The results obtained were analyzed using the Shannon-Wiener Diversity Index (H'), the Evenness index (E) and the Simpson Dominance Index (C). A total of 747 individuals from 12 types of kelulut were found in this study. The secondary forest gets the highest H' value (1.869), followed by the primary forest (1.672) and ecosystem restoration area (1.159). The diversity of stingless bees is higher in forested areas due to environmental factors such as temperatures within the normal range (25.5-28.9°C), the availability of trees for nesting, and the abundance of food sources from flowers.
Efektivitas Ekstrak Etanol Bawang Putih (Allium Sativum L.) Sebagai Larvasida Alami Pada Larva Aedes Aegypti Yolanda Oktavianingsih; Sari Rahmayanti; Firman Saputra
Jurnal Kesehatan Republik Indonesia Vol 3 No 5 (2026): JKRI - April 2026
Publisher : PT. INOVASI TEKNOLOGI KOMPUTER

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Abstract

Penyakit Demam Berdarah Dengue (DBD) adalah penyakit menular karena infeksi virus dengue (DENV). Nyamuk Aedes aegypti yang terinfeksi virus dengue berperan sebagai vektor perantara. Penggunaan larvasida merupakan salah satu upaya pencegahan penyakit ini. Larvasida alami diharapkan dapat mengurangi dampak negatif larvasida sintetik. Bawang putih mengandung senyawa metabolit sekunder, seperti flavonoid, alkaloid, tanin, saponin dan organosulfur yang berpotensi sebagai larvasida. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak etanol bawang putih (Allium sativum L.) sebagai larvasida alami pada larva Aedes aegypti. Hewan uji yang digunakan adalah larva Aedes aegypti instar III. Kelompok uji berupa ekstrak etanol bawang putih (Allium sativum L.) dengan 5 dosis, yaitu 375 ppm, 500 ppm, 625 ppm, 750 ppm dan 1000 ppm, serta kontrol negatif berupa aquades 100 ml dan kontrol positif berupa temephos 0,02 ppm. Pengujian ini diulang sebanyak 3 kali dalam satu waktu selama 24 jam. Ekstrak etanol bawang putih mengandung senyawa metabolit sekunder berupa flavonoid, alkaloid, tanin, saponin dan organosulfur. Persentase mortalitas larva yang dihasilkan oleh ekstrak etanol bawang putih dosis 375 ppm, 500 ppm, 625 ppm, 750 ppm dan 1000 ppm secara berturut-turut adalah 14,67%, 22,67%, 37,33%, 53,33% dan 70,67%, sedangkan pada kontrol negatif dan positif masing-masing adalah 0% dan 100%. Uji Kruskal-Wallis menunjukkan bahwa terdapat perbedaan nilai rata-rata mortalitas yang signifikan antar kelompok uji (Sig.= 0,003). Nilai LC50 adalah 785,391 ppm (LC50>750 ppm) yang artinya aktivitas larvasida tergolong tidak aktif, sedangkan LC90 adalah 1452,280 ppm. Ekstrak etanol bawang putih (Allium sativum L.) memiliki aktivitas larvasida, tetapi dosis yang diuji belum efektif sebagai larvasida alami pada larva Aedes aegypti apabila dibandingkan dengan kontrol positif
Co-Authors Ageresya Ester Evelin Br Sibarani Didin Joharrudin Kustiati Patrisia Lindawati Rahmawati Rahmawati Sari Rahmayanti Yolanda Oktavianingsih