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All Journal Journal of Indonesian Dental Association Jurnal Material Kedokteran Gigi Journal Research of Social Science, Economics, and Management Indonesian Journal of Dentistry Jurnal Abdimas Indonesia
Etny Dyah Harniati
Universitas Muhammadiyah Semarang
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Mouthwash Potential of Ethanol Extract of Ajwa Date Seeds (Phoenix dactylifera L.) against Porphyromonas gingivalis Bacteria Mauriska Nada Silmi Almisky; Etny Dyah Harniati; Steffi Triany Arnov; Ariyani Faizah
Journal of Indonesian Dental Association Vol. 9 No. 1 (2026): April
Publisher : Indonesian Dental Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jida.v9i1.1535

Abstract

Introduction: Periodontal disease is a major oral health problem worldwide, with Porphyromonas gingivalis recognized as one of the primary causative bacteria. Ajwa date seeds (Phoenix dactylifera L.) have potential for development as a natural mouthwash due to their antibaterial properties, which are attributed to various bioactive compounds, including flavonoids, tannins, and saponins. Objective: To evaluate the antibacterial effect of a mouthwash containing ethanol extract of ajwa date seeds (Phoenix dactylifera L.) against the growth of Porphyromonas gingivalis. Methods: This laboratory experimental study employed a post-test-only control group design. The samples were divided into five groups: a positive control, a negative control, and three treatment groups receiving mouthwash containing ethanol extract of ajwa date seeds at concentrations of 3.25%, 6.5%, and 12.5%. Antibacterial activity was evaluated using the disc diffusion method on Mueller–Hinton Agar (MHA), with six replicates for each group. Data were analyzed using one-way ANOVA followed by Tukey's Honestly Significant Difference (HSD) post hoc test. Results: Mouthwash containing ethanol extract of ajwa date seeds inhibited the growth of Porphyromonas gingivalis, as demonstrated by the formation of inhibition zones around the discs. A concentration-dependent increase in the diameter of the inhibition zone was observed. The mean inhibition zone diameters were 10.175 mm, 12.300 mm, and 14.442 mm for the 3.25%, 6.5%, and 12.5% extract concentrations. Conclusion: Mouthwash containing ethanol extract of ajwa date seeds (Phoenix dactylifera L.) significantly inhibited the growth of Porphyromonas gingivalis. The antibacterial activity in 12.5% concentration demonstrating the greatest inhibitory effect.  
Co-Authors Ageng Wicaksono Ariyani Faizah Budiono Budiono Haura Hafizhah Zain Ismaya Aria Sari Lira Wiet Jayanti Lukman Sikha Prasetyo Mauriska Nada Silmi Almisky Propana Fema Pamungkas Ratna Sulistyorini Retno Kusniati Rini Dharmastiti Risyandi Anwar Ryan Mahardiansyah Steffi Triany Arnov Suaeni Kurnia Wirda W. Widjijono Wika Putri Rizkiah Yanuarita Tursinawati Yasinia Annisa Purbomurti