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Seahorse (Hippocampus sp): opportunities in assisted reproduction with nanotechnology approach Trisnawati Mundijo; Yurnadi Hanafi Midoen; Radiana Dhewayani Antarianto
Indonesian Archives and Biomedical Sciences Vol 1 No 1 (2021): Indonesian Archives and Biomedical Sciences Vol 1(1): 2021
Publisher : Konsorsium Ilmu Biomedik Indonesia (KIBI)

Seahorse (Hippocampus sp) is the unique fish, because the male is pregnancy. The seahorse not only given an economic value but also have a potential drug for medical use, which is have a steroid an aphrodisiac for fertility. The previous studies have reported the function seahorse extract and have been studied both in vitro and in vivo to know the effects from the natural product. Nowadays, infertility caused many factors, such as a factor from the male including spermatogenesis and testis disorder. In decade, there is the treatment for infertility used GnRH therapy, but it still controversy between the impact in reproductive system and needed the study. GnRH in vertebrate has a G receptor from kisspeptin gene to transcription promote and suggested a key regulator reproductive with a kiss 1 gene, which is the same function in mammals and fishes. In this condition, it is suggested a bioactive compound in seahorse can be investigated to replace a treatment infertility in male with GnRH therapy. It can be done through drug delivery systems with several carrier systems, such as nanoparticles. In the future, bioactive compound from seahorse needed the study to explore a potential drug in medical use including the efficiency and efficacy for fertility in human with nanotechnology approach.

PENGARUH PENYUNTIKAN DOSIS MINIMAL DEPOT MEDROKSIPROGESTERON ASETAT (DMPA) TERHADAP BERAT BADAN DAN KIMIA DARAH TIKUS JANTAN GALUR SPRAGUE-DAWLEY Yurnadi, Yurnadi; Suryandari, Dwi Anita; Moeloek, Nukman
Makara Journal of Science Vol. 13, No. 2
Publisher : UI Scholars Hub

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Effect of Injection Minimal Dosages of Depot Medroxyprogesterone acetate (DMPA) to Body Weight and Blood Chemistry Male Rat Strain Sprague-Dawley. Many family planning program focus more on men. Until now, vasectomy has been the commonly used method for male contraception. However, this method creates inconvenience such as irreversibility and psychological problems. One of the alternatives contraception is the combination of depot medroxyprogesterone acetate (DMPA) and androgen. The minimum dosage of DMPA could suppress testosterone level that leads to reduced spermatogenesis and sperm viability. Nevertheless, until now it is not known whether minimum dosages of DMPA have an effect to body weight and blood chemistry. Therefore, this research aimed at determinate the effect of minimal dosages of DMPA to body mass and blood chemistry using male rats (Rattus norvegicus L.) strain Sprague-Dawley as model. This research using completely randomized design, unequal size sample, castration treatments and several doses DMPA (1.25, 0.625, and 0.313 milligram). Injecting of DMPA conducted intramuscularly on week 0 and week 12. Normality/homogeneity Data normality were analyzed before ANOVA test. Then, abnormal data were tested using Kruskal-Wallis test. The result shows that injection of DMPA in various doses do not have an effect on body weight and blood chemistry such as erytrocytes, haemoglobin, hematocrite, HDL, LDL, total cholesterol, SGOT, SGPT and triglyseride) (p>0,05). Furthermore, it is concluded that that no effect of minimal dosages of DMPA to body mass and blood chemistry of rat.

COMBINATION OF DEPOT MEDROXY PROGESTERONE ACETATE AND JAVANESE LONG PEPPER EXTRACT ON BODY WEIGHT, HEMATOLOGY, AND BLOOD BIOCHEMISTRY AS A SAFE CONTRACEPTION MODEL Yurnadi, Yurnadi; Asmida, Yoel; Suryandari, Dwi Anita; Moeloek, Nukman
Makara Journal of Science Vol. 15, No. 2
Publisher : UI Scholars Hub

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The development of male hormonal contraception is based on a decrease in sperm concentration without affecting libido and sexual potency. The combination of depot medroxy progesterone acetate (DMPA) + extract of Javanese long pepper (JLP) with dosages of 0.94 mg and 1.88 mg decreases the concentration of spermatozoa. However, it remains unknown whether the combination influences body weight, hematology, and blood biochemistry. Therefore, it is necessary to investigate the effect of DMPA + JLP extracts on the body weight, hematology, and blood biochemistry of male rats (Rattusnorvegicus L.) using Sprague-Dawley strains. The research uses a completely randomized design (CRD); one group control and two treatment groups. In the first group, the castrated rats were given oral administration extracts of JLP (CJ) with doses of 0, 0.94, 1.88, 2.82, and 3.76 mg. In the second group, the rats were injected with 1.25 mg DMPA and given an oral administration extract of JLP. Injection was given in week-0 and 12. Administration was conducted every day from week 7-18. Analysis of the normality and homogeneity of data is done before the ANOVA test. Data that is abnormal and not homogeneous are tested with non-parametric statistical Kruskal-Wallis. This study shows that the combination of minimal doses of DMPA and administration variousdoses of extracts of JLP does not affect body weight and hematology (erythrocyte, hemoglobin, hematocrite), and the blood biochemistry of rats, such as the values of SGPT, SGOT, HDL, and triglycerides (p < 0.05), but rather the total cholesterol and LDL (p < 0.05). Furthermore, it is concluded that the combination of the minimal dosage of DMPA and weaned various dosages of JLP extracts affect the total value and LDL cholesterol but do not influence body weight, nor hematology and blood biochemistry. Such combinations can be drawn on for asafe male contraceptive model t by taking into account the value of the total cholesterol and LDL during its use.

PENGARUH PENYUNTIKAN DOSIS MINIMAL DEPOT MEDROKSIPROGESTERON ASETAT (DMPA) TERHADAP BERAT BADAN DAN KIMIA DARAH TIKUS JANTAN GALUR SPRAGUE-DAWLEY Yurnadi, Yurnadi; Suryandari, Dwi Anita; Moeloek, Nukman
Makara Journal of Science Vol. 13, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Effect of Injection Minimal Dosages of Depot Medroxyprogesterone acetate (DMPA) to Body Weight and Blood Chemistry Male Rat Strain Sprague-Dawley. Many family planning program focus more on men. Until now, vasectomy has been the commonly used method for male contraception. However, this method creates inconvenience such as irreversibility and psychological problems. One of the alternatives contraception is the combination of depot medroxyprogesterone acetate (DMPA) and androgen. The minimum dosage of DMPA could suppress testosterone level that leads to reduced spermatogenesis and sperm viability. Nevertheless, until now it is not known whether minimum dosages of DMPA have an effect to body weight and blood chemistry. Therefore, this research aimed at determinate the effect of minimal dosages of DMPA to body mass and blood chemistry using male rats (Rattus norvegicus L.) strain Sprague-Dawley as model. This research using completely randomized design, unequal size sample, castration treatments and several doses DMPA (1.25, 0.625, and 0.313 milligram). Injecting of DMPA conducted intramuscularly on week 0 and week 12. Normality/homogeneity Data normality were analyzed before ANOVA test. Then, abnormal data were tested using Kruskal-Wallis test. The result shows that injection of DMPA in various doses do not have an effect on body weight and blood chemistry such as erytrocytes, haemoglobin, hematocrite, HDL, LDL, total cholesterol, SGOT, SGPT and triglyseride) (p>0,05). Furthermore, it is concluded that that no effect of minimal dosages of DMPA to body mass and blood chemistry of rat.

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