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All Journal Medical Journal of Indonesia The Indonesian Biomedical Journal Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Acta Biochimica Indonesiana Current Research on Biosciences and Biotechnology Indonesian Archives of Biomedical Research (InABR) Indonesian Journal of Cancer Makara Journal of Health Research Makara Journal of Science
Radiana Dhewayani Antarianto
Departemen Histologi, FK Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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Increasing the capacity of natural killer (NK) cells in fighting advance stage ovarian cancer: A cellular immunotherapy minireview Radiana Dhewayani Antarianto; Fransisca Dela Verna; Lady Feren Pangjaya; Sanya Khaerunnisa; Dinda Shezaria; Tricia Dewi Anggraeni
Current Research on Bioscences and Biotechnology Vol. 2 No. 1 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2020.2.1/SPNZ3413

Abstract

Global Cancer Statistics in 2018 estimated 300,000 new cases of ovarian cancer with 152,000 mortality rate each year. The Indonesian Society of Gynecologic Oncology reported 30% of gynecologic cancer is ovarian cancer, which has a 125, 000 mortality rate each year. Ovarian cancer data in Indonesia showed that 70% of patients were diagnosed with ascites or metastasis beyond the ovaries (stage III or IV). Ovarian cancer is an immunogenic disease with an immunotherapy intervention on the horizon. To assess the potency of stimulated NK cells as ovarian cancer cellular immunotherapy, literature search was collected from NCBI, ScienceDirect and Pubchem database. A total of 19 articles relevant to our search terms were included in this review. NK cells from ovarian cancer ascites exhibit low cytotoxic efficacy but can be restimulated using IL-2 or IL-15. An in vitro study that incubated NK cells with an IL-15 fusion protein enhanced the function of the ovarian cancer ascites’ NK cells or the healthy NK cells against the ovarian cancer ascites cells. Human IL-12-, IL-15- and IL-18-induced memory like (CIML) NK cells, has been proven to increase the elimination of xenograft human ovarian cancer cells over a long period of time in a mouse model. CIML NK cells also showed higher NK cell expansion and an enhanced function in the ovarian cancer ascites’ microenvironment, which was immunosuppressive. Phase I-II clinical trials on NK cell-based adoptive cellular therapies demonstrated limited clinical benefit. The major challenges are obtaining persistent NK cells with anticancer activity.
The Role of artificial intelligence in designing antibody-based therapy for Covid-19 Wahyu Hidayati; Radiana Dhewayani Antarianto
Indonesian Archives and Biomedical Sciences Vol 1 No 1 (2021): Indonesian Archives and Biomedical Sciences Vol 1(1): 2021
Publisher : Konsorsium Ilmu Biomedik Indonesia (KIBI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1140.403 KB) | DOI: 10.55392/indarcbiores.v1i1.4

Abstract

For several decades ago, passive immunization has already proven its ability to treat some diseases, especially pandemic diseases. On the contrary, after antibiotics discovery, the usage of passive immunization becomes neglected. Nowadays, we face a pandemic situation, COVID-19, which needs the possible treatment to save patients lives while medicines and vaccines are under development. By learning from history, passive immunization seems to be the best choice to save patient lives. As a kind of passive immunization, antibody-based therapy successfully treats diseases, including infectious diseases. Several antibody-based therapies are developed, as vast as the technology development, especially after the genetic codes announced. This article highlighted the influence of genomics tools, which helps researchers develop various platforms in developing monoclonal antibodies with high safety and efficiency in production and application.
Biosensor development as an alternative test for Leptospirosis diagnosis: a systematic review Susanti Susanti; Radiana Dhewayani Antarianto
Indonesian Archives and Biomedical Sciences Vol 1 No 2 (2021): Indonesian Archives of Biomedical Research (InABR). 1(2): 2021
Publisher : Konsorsium Ilmu Biomedik Indonesia (KIBI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (818.951 KB) | DOI: 10.55392/indarcbiores.v1i2.22

Abstract

Leptospirosis is a zoonotic disease caused by pathogenic bacteria from the genus Leptospira that can attack livestock, wild animals, and humans. The diagnosis of leptospirosis is currently carried out by Leptospira culture, Microscopic Agglutination Test (MAT), Enzyme Linkage Immunosorbent Assay (ELISA), and Polymerase Chain Reaction (PCR). This test requires a specific laboratory, long test time, experienced personnel, a lot of equipment and is expensive and difficult to apply in the field. Biosensor technology is a necessary method of disease diagnosis to detect biomolecules such as protein and bacteria biomarkers. The purpose of this article is to provide information on the development of biosensors as an alternative test for the diagnosis of leptospirosis. The method used is a systematic review using the PRISMA protocol. The results showed that the electrochemical biosensor with monoclonal anti-LipL32 and ssDNA probe specific to the LipL32 gene of Leptospira had advantages for leptospirosis diagnosis because it was cheap, accurate, portable, small test equipment, and capable of detecting leptospirosis. Optical biosensors (specifically lateral flow systems, magnetogenosensors and paper fluidic devices) can detect Leptospira bacteria with sensitive, specific, easy manipulation, and users can get fast visual test results within minutes. This biosensor technology can be used as a promising alternative diagnostic method for the diagnosis of leptospirosis with simplicity, high sensitivity, fast detection time, low cost, and portable so that it is easy to apply in the field.
CD34+ UCB stem cells attenuate TGF-β signaling and inhibit liver fibrosis: A new avenue for liver cirrhosis-carcinogenesis prevention Septiana, Wahyunia Likhayati; Antarianto, Radiana Dhewayani; Louisa, Melva; Jusuf, Ahmad Aulia; Barasila, Atikah Chalida; Pawitan, Jeanne Adiwinata; Fasha, Iqbal
Makara Journal of Health Research Vol. 24, No. 2
Publisher : UI Scholars Hub

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Abstract

Background: The liver microenvironment plays a key role in liver fibrosis and carcinogenesis. This study aimed to fill the gap in knowledge on the interaction between hepatic stellate cells and endothelial progenitor cells with biomarkers of liver fibrosis and/or carcinogenesis, including Col1A1, TGF-β, and tenascin-C. Methods: CD34+ stem cells were isolated from umbilical-cord-blood mononuclear cells. 2D and 3D co-culture of CD34+ UCB SCs and LX2 was performed. The cells were incubated in a CO2 incubator for three days. Morphological observation, qRT-PCR of TGF-β1 and COL1A1, and immunocytochemistry of tenascin-C were performed. Results: CD34+ UCB SCs were viable in the 2D and 3D co-culture for 24 h. 3D co-culture of CD34+ UCB SCs and LX2 inhibited in vitro liver fibrosis by lowering Col 1A1 expression as compared to control. We observed lower TGF-β expression in 3D co-culture on days 1 and 2 followed by higher expression of TGF-β on day 3. 2D co-culture of CD34+ UCB SCs and LX2 showed a different level of COL1A1 and TGF- β expression compared with 3D co-culture. Spheroids from 2D co-culture of CD34+ UCB SCs and LX-2 showed immunoreactivity against tenascin-C. Conclusion: Interaction between LX-2 and CD34+ UCB SCs in 3D co-culture inhibits in vitro liver fibrosis. The viability of CD34+ UCB SCs is essential for attenuation of TGF-β signaling in LX-2.
The impact of fasting toward oxidative stress marker in the liver and plasma of new zealand white rabbit Novi Silvia Hardiany; Stephanie Gosal; Damayanti Angelina; Engelbert Julyan Gravianto; Radiana Dhewayani Antarianto
Acta Biochimica Indonesiana Vol. 5 No. 2 (2022): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.112

Abstract

Background: Fasting may increase the activity of endogenous antioxidants and protect against oxidative stress. However, the effects of different fasting durations on the liver have not been reported. Objective: The purpose of this study is to determine the effect of intermittent and prolonged fasting on oxidative stress markers in the liver tissue and plasma of New Zealand White rabbits. Methods: New Zealand White rabbits were divided into three groups: control, intermittent fasting (IF), and prolonged fasting (PF), with each group consisting of five rabbits. The control group was provided with food ad libitum; the IF group fasted for 16 hours, while the PF group fasted for 40 hours, followed by an eight-hour non-fasting period for six days. In liver tissue and plasma, oxidative stress indicators (catalase, carbonyl, GSH) were evaluated. Results: In the IF group, liver GSH was significantly higher than in the control group. However, neither liver carbonyl nor catalase levels changed significantly in the IF group. In the IF group, plasma carbonyl was significantly lower than in the PF group. In addition, there was no significant differences between groups in plasma catalase and GSH levels. Conclusion: Intermittent fasting and prolonged fasting could significantly increase liver GSH levels of New Zealand White rabbits. In addition, intermittent fasting is more effective than prolonged fasting at preventing oxidative stress.
Seahorse (Hippocampus sp): opportunities in assisted reproduction with nanotechnology approach Trisnawati Mundijo; Yurnadi Hanafi Midoen; Radiana Dhewayani Antarianto
Indonesian Archives and Biomedical Sciences Vol 1 No 1 (2021): Indonesian Archives and Biomedical Sciences Vol 1(1): 2021
Publisher : Konsorsium Ilmu Biomedik Indonesia (KIBI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.323 KB) | DOI: 10.55392/indarcbiores.v1i1.7

Abstract

Seahorse (Hippocampus sp) is the unique fish, because the male is pregnancy. The seahorse not only given an economic value but also have a potential drug for medical use, which is have a steroid an aphrodisiac for fertility. The previous studies have reported the function seahorse extract and have been studied both in vitro and in vivo to know the effects from the natural product. Nowadays, infertility caused many factors, such as a factor from the male including spermatogenesis and testis disorder. In decade, there is the treatment for infertility used GnRH therapy, but it still controversy between the impact in reproductive system and needed the study.  GnRH in vertebrate has a G receptor from kisspeptin gene to transcription promote and suggested a key regulator reproductive with a kiss 1 gene, which is the same function in mammals and fishes. In this condition, it is suggested a bioactive compound in seahorse can be investigated to replace a treatment infertility in male with GnRH therapy.  It can be done through drug delivery systems with several carrier systems, such as nanoparticles. In the future, bioactive compound from seahorse needed the study to explore a potential drug in medical use including the efficiency and efficacy for fertility in human with nanotechnology approach. 
Isolation and Characterization of Adipose-Derived Mesenchymal Stem Cell Exosomes: an In-Vitro Study Jessica Fiolin; Ismail Hadisoebroto Dilogo; Radiana Dhewayani Antarianto; Ludwig Andre Pontoh
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 16 No 2 (2022): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v16i2.4867

Abstract

Exosomes from Mesenchymal Stem Cells (MSC) is currently one of the highlighted research due to its more specific action on the target tissue. Exosome is one of the MSC Extracellular Vesicle (EVs) that acts as a mediator in the cellular communication. However, not so many literatures have succeed in elaborating its isolation and characterization process. This study aimed to explain the method used in exosomes isolation from Adipose-Derived MSC (ASC) and elaborate its characteristics. This is an in-vitro study performed in the Stem Cell and Tissue Engineering (SCTE) Indonesia Medical Education and Research Institute (IMERI) laboratory. Ultracentrifugation method was performed to isolate exosome from ASC. The exosome were characterized based on its particle size, morphology, and CD63 and CD81 expression for its purity. We were able to isolate sterile exosome from ASC by performing differential ultracentrifugation. The mean size of exosome was 88.7 nm ± 40 nm SD and showed expression of CD63 and CD81. Exosome was successfully isolated from ASC using ultracentrifugation method and characterization following the MISEV standard should be implemented in order to meet the therapeutic efficacy and safety issues as a regenerative agent.
The Use of Cell-penetrating Peptide for Delivery of Recombinant Transcription Factor DNA into Primary Human Fibroblast Melinda Remelia; Budiman Bela; Silvia Tri Widyaningtyas; Radiana Dhewayani Antarianto; Nuzli Fahdia Mazfufah; Jeanne Adiwinata Pawitan
Molecular and Cellular Biomedical Sciences Vol 7, No 1 (2023)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v7i1.279

Abstract

Background: Reprogrammed cell therapy has not been applied for clinical purposes due to the malignancy issue. The aim of this study was to design the recombinant vector of the transcription factors and analyze the effectiveness of cell-penetrating peptide delivering system for human primary fibroblast transfection to avoid the malignancy issue.Materials and methods: The constructions of CCAT/enhancer binding protein alpha (CEBPA), hepatocyte nuclear factor 4 alpha (HNF4A), nuclear receptor subfamily 1 group I member 2 (NR1I2) were confirmed with DNA digestion and sequencing. Breast reduction (BRED) and palate (PAL) tissue were used as human primary fibroblast sources. The transcription factors were delivered into BRED and PAL with recombination of avian leukosis sarcoma virus (ALSV), human immunodeficiency virus (HIV) matrix, and regulator of expression of virion proteins (Rev) (ALMR), tagged with enhanced green fluorescence protein (eGFP). Post-transfection cells were then cultivated with optimized medium. Gene expression was measured with quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR).Results: Gene expression levels of CEBPA, HNF4A, NR1I2, glutamate-ammonia ligase (GLUL), albumin (ALB), and cytochrome P450 (CYP) were increased. Transfection with ALMR, which were more efficient in BRED than PAL fibroblasts may have the advantage in autologous cell therapy for elderly patients.Conclusion: Transfection of transcription factors to human primary fibroblast may be performed by using constructions of plasmid as designed in this study.Keywords: recombinant plasmid, hepatocyte-like cells, primary fibroblasts, recombinant peptide, cell reprogramming, autologous cells therapy
Comparison of the effect of Sumbawa honey and sucrose as extracellular cryoprotectants on viability, morphology, and phenotype stability of CD34+ hematopoietic stem cells from umbilical cord blood Samuel Febrian Wijaya; Retno Lestari; Inna Rahmawati; Imelda Rosalyn Sianipar; Robby Nuraditya; Iqbal Fasha; Gita Pratama; Radiana Dhewayani Antarianto
Current Research on Bioscences and Biotechnology Vol. 5 No. 1 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.5.1/CBWGKPBX

Abstract

Cryopreservation is a common practice for the long-term storage and maintenance of the quality of Hematopoietic Stem Cells (HSC) from Umbilical Cord Blood (UCB). Cryomedium containing 10% DMSO is the standard intracellular cryoprotectant agent (CPA) in HSC cryopreservation. However, DMSO is toxic to both cells and patients during transplantation. Therefore, the concentration of DMSO needs to be reduced by adding extracellular CPAs, such as sucrose or Sumbawa honey. The objective of this study was to compare the ability of Sumbawa honey and sucrose as extracellular CPAs to protect HSC CD34+ during cryopreservation. This in vitro study was designed using cryomedium consisting of 10% DMSO as a control, 5% DMSO + 5% Sumbawa honey, and 5% DMSO + 5% sucrose. The results showed that the cryoprotectant containing 5% DMSO + 5% Sumbawa honey had a positive effect and a significant difference (p<0.05) compared with 5% DMSO + 5% sucrose on the viability and morphology of HSC. However, the mean reduction in phenotype stability as indicated by the decrease in percentage CD34+ in the 10% DMSO (6.90 ± 8.60), 5% DMSO + 5% sucrose (10.60 ± 9.20), and 5% DMSO + 5% Sumbawa honey (8.60 ± 11.50) showed no significant difference (p>0.05). In conclusion, the combination of DMSO and Sumbawa honey was able to maintain the viability, morphology, and phenotype stability of HSC. Therefore, honey can be used as an alternative cryoprotectant for the cryopreservation of hematopoietic stem cells.
Effect of Calorie Restriction on the Expression of Sirtuin1 as an Antiaging Biomarker Penantian, Raya Makarim; Antarianto, Radiana Dhewayani; Hardiany, Novi Silvia
Makara Journal of Science Vol. 27, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Calorie restriction (CR) is the most effective method for delaying aging and preventing the onset of age-related diseases. Sirtuins constitute a family of nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylases. Their activity can be regulated by NAD+/NADH levels, which are influenced by nutrient intake, a variable acted upon by CR. This review elaborates on the link between CR and sirtuin1 (SIRT1). It retrieved articles from several sources, such as ClinicalKey, PubMed, and ScienceDirect. It discusses the up-to-date knowledge of how SIRT1 acts as a nutrient sensor and regulator of molecular mechanisms. These mechanisms include the control of the cell cycle, enhancing mitochondrial quality control, activating fatty acid oxidation, and stimulating anti-inflammatory effects. Disruptions in the aforementioned mechanisms are the basis of aging. CR increases the expression of SIRT1, which enhances the biogenesis and dynamics of mitochondria, resulting in an antiaging effect. In CR, SIRT1 is activated and stimulates different pathways, especially those related to mitochondrial activity and effectiveness, leading to an antiaging effect in collaboration with other antiaging biomarkers
Co-Authors Achmad Fauzi Kamal Aditya, Robby Nur Agian Jeffilano Barinda Ahani, Ardhi Rahman Ahmad Aulia Ahmad Aulia Jusuf Angka, Rebecca Noerjani Aprilya, Dina Aria Kekalih Asrinda, Indria Atikah Chalida Barasila Barasila, Atikah Chalida Budiman Bela Canintika, Anissa Feby Chyntia Olivia Maurine Jasirwan, Chyntia Olivia Maurine Cosphiadi Irawan Damayanti Angelina Deby Deby Dinda Shezaria Engelbert Julyan Gravianto Fadhillah, Muhamad Rizqy Fasha, Iqbal Fitri Octaviana Fransisca Dela Verna Gita Pratama Heri Suroto Imelda Rosalyn Sianipar Inna Rahmawati Iqbal Fasha Iqbal Fasha Ismail Hadisoebroto Dilogo Jeanne Adiwinata Pawitan Jeannne Adiwinata Pawitan Jeo, Wifanto Saditya Jessica Fiolin Khatimah, Nurul Gusti Kousar, Iqra Lady Feren Pangjaya Lia Damayanti Ludwig A. Pontoh Mahmood, Amer Melinda Remelia Melva Louisa Mundijo, Trisnawati Ni Ken Ritchie, Ni Ken Novi Silvia Hardiany Nur Aditya, Robby Nuraditya, Robby Nurjati Chairani Siregar Nuzli Fahdia Mazfufah Penantian, Raya Makarim Puspita Eka Wuyung Rahmawati, Inna Rahyussalim Rahyussalim, Rahyussalim Retno Lestari Retno Lestari Robby Nuraditya Samuel Febrian Wijaya Sanya Khaerunnisa Shimizu, Ippei Silvia Tri Widyaningtyas Stephanie Gosal Sudoyo, Aru Wisaksono Supraja Dwiyono Susanti Susanti Tricia Dewi Anggraeni Wahyu Hidayati Wahyu Widodo Wahyunia Likhayati Septiana Wawaimuli Arozal Wijaya, Samuel Febrian Yurnadi Yurnadi