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Progesterone decrease plasma membrane in human sperm with subnormal hypoosmotic swelling test scores Sisca, Sisca; Yunaini, Luluk; Pujianto, Dwi Ari
Universa Medicina Vol 38, No 1 (2019)
Publisher : Faculty of Medicine, Trisakti University

BackgroundProgesterone (P4) is known as a female hormone affecting oocyte maturation and developing uterine wall. A proteomic study identified several receptors including P4 receptors on human sperm. The role of P4 in human sperm cells remains unknown as to whether P4 has non-genomic effects on human sperm. The present study aims to determine the effect of progesterone (P4) on the hyperactivated motility and membrane integrity of human sperm cells.MethodsSemen from normal individuals was obtained from donors. The semen was washed by gradient density centrifugation. P4 was added to each semen sample to final concentrations of 0 (control), 250, 500, 750 and 1000 ng/mL. After the sample treatment was completed, the sperm membrane integrity was assessed with the hypoosmotic swelling test (sodium citrate dihydrate and D-fructose) and the hyperactivated sperm motility parameter was determined with the Computer Assisted Sperm Analyzer [CASA] (Hamilton Thorne, IVOS II, USA). The percentage was then compared between the treatment groups and the control group. The percentage differences were analyzed with the Sigmastat version 2.0 statistical program.ResultsAdministration of P4 increased sperm hyperactivated motility when compared with the control group at a concentration of 500 ng/mL, but the increase was statistically not signicant (p>0.05). In contrast, P4 decreased sperm membrane integrity significantly (p=0.042). And the mean of plasma membrane integrity in all groups was subnormal hypoosmotic swelling test score. ConclusionProgesterone administration tends to increase sperm hyperactivated motility. The integrity of plasma sperm membrane was affected by progesterone.

Pengaruh Hormon Androgen terhadap Ekspresi Gen CD52 di Epididimis Mencit (Mus musculus) Silvani Permatasari; Dwi Ari Pujianto; Astrid Teresa
Jurnal Kesehatan Andalas Vol 9, No 2 (2020): Online June 2020
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v9i2.1224

Androgen has an important role in regulation epididymal sperm maturation Epididymal sperm maturation is occurs via interactions between sperm and proteins secreted by epididymal genes that are specifically expressed in a region-specific manner. Mice CD52 exhibited expression in all regions in epididymis. However, the regulation of its expression by androgen is needed to be known as a gene candidate that involved in sperm maturation. Objective: This study was aimed to analyze regulation of mice CD52 expression by androgen and a bioinformatics analysis was performed to predict functional domains of CD52. Methods: bioinformatics analysis using Motif Scan. For experiment, mice divided into 5 groups. The groups were control was injected ethanol 0,1 mL/day and others groups were 3, 5, 10, and 15 day used a androgen receptor antagonist, flutamide that dilute in ethanol at a dose of 50 mg/kg/day. And then quantitative real-time RT-PCR was used to analyse expression of CD52 and it’s normalized to Beta actin. Result: CD52 contained a two potential phosphorylation sites for protein kinase C and casein kinase II, N-myristoylation, and N-glycosylation that showed CD52 is secretory protein and predict it’s attached to sperm membrane. Expression of CD52 was decrease about 93% than control. Conclusion: CD52 expression depend on androgen.Keywords: androgen; epididymis; sperm maturation; expression of CD52

Phosphorylated Ataxia Telangiectasia Mutated (pATM) Enzyme-Linked Immunosorbent Assay (ELISA) for Predicting Radiation Induces Normal Tissue Toxicity in Radiotherapy Patients: A Systematic Review Sofiati Purnami; Viria Agesti Suvifan; Dwi Ramadhani; Yanti Lusiyanti; Darlina Darlina; Nastiti Rahajeng; Mukh Syaifudin; Dwi Ari Pujianto; Retno Widowati
Indonesian Journal of Cancer Vol 17, No 3 (2023): September
Publisher : http://dharmais.co.id/

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33371/ijoc.v17i3.971

Background: An adverse normal tissue response, such as normal tissue toxicity (NTT), is present in radiotherapy (RT) patients and can limit the effectiveness of the RT treatment. Identifying patients with adverse tissue responses before RT had clinical benefits and individual radiosensitivity (IRS) is considered an important factor in NTT incidences. Therefore, this systematic study aimed to determine the possibility of using phosphorylated Ataxia Telangiectasia Mutated (pATM) Enzyme-Linked Immunosorbent Assay (ELISA) to predict NTT in RT patients.Methods: A comprehensive data search was conducted in three electronic databases, namely PUBMED CENTRAL, ScienceDirect, and SCOPUS. The quality of relevant publications was independently evaluated using the PICO (participants, intervention/exposure, comparison, and outcome) approach.Results: : A total of 47 articles were retrieved, 41 of which were assessed based on the titles and abstracts. Furthermore, 39 articles were excluded, and 2 were included in this study.Conclusions: The phosphorylated ATM ELISA on lymphocytes showed promising results for IRS prediction in RT patients. However, these assumptions should be validated on a larger RT patient cohort.

Photochemical and bioactivity examination of fractionated saluang belum root extract (Lavanga sarmentosa) on in-vitro human sperm motility Silvani Permatasari; Syarpin; Dwi Ari Pujianto
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 14, No 2, (2023)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol14.Iss2.art3

Background: Saluang belum (Lavanga sarmentosa) is one of Kalimantan's typical plants, which is as efficacious as a traditional medicine to increase sexual activity and male fertility. Based on previous studies, the content of flavonoid and steroid in 70% ethanol extract of L. sarmentosa were able to affect sperm quality of mice. Studies related to L. sarmentosa are still limited for phytochemical test and their bioactivity on human spermatozoa motility in vitro.Objective: This study is to perform phytochemical tests of compound content in fractionation with eluents of high and low polarity, namely methanol and chloroform, and then to test their bioactivity on the motility of human spermatozoa in vitro.Methods: L. sarmentosa was extracted with 96% ethanol and fractionated by using a vacuum chromatography column with chloroform and methanol as the eluent. Then obtained samples were analysed by a quantitative phytochemical test. The samples used in-vitro human spermatozoa were divided into eleven groups: control group, group administered with L. sarmentosa extract eluent chloroform of 10, 50, 100, 500, and 1000 ng/mL, and same concentration with extract eluent methanol. Furthermore, the sperm motility was analysed by using a Computer Assisted Sperm Analyser (CASA). Results: The methanol and chloroform fraction of L. sarmentosa root extract contained metabolites, namely terpenoids, flavonoids, steroids, alkaloids, saponins, and tannins. The sperm motility increased significantly at the treatment group of the methanol and chloroform fractions compared to the control group. There was a significant difference between the sperm motility incubated with methanol and that with chloroform fraction at concentration 500 and 1000 ng/mL.Conclusion: The results of sperm motility were higher in the methanol fraction than those in the chloroform fraction.

The Predictive Value of Sperm Mitochondrial DNA Copy Number on Male Infertility: A Systematic Review and Meta-Analysis Aghnia Yuslicha Yuniar; Dwi Ari Pujianto
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 8 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i8.1362

Background: The integrity and quantity of mitochondrial DNA (mtDNA) in spermatozoa are considered critical for male reproductive potential. While qualitative damage like deletions has been linked to infertility, the significance of quantitative changes, specifically mtDNA copy number (mtDNAcn), remains debated. This study aimed to systematically review and meta-analyze the existing evidence to determine the association between sperm mtDNAcn and male infertility. Methods: We conducted a systematic search of PubMed, Science Direct, and Scopus databases for observational studies published between January 2014 and December 2024. The search included studies that compared sperm mtDNAcn between infertile men, who were diagnosed with conditions such as oligozoospermia, asthenozoospermia, and teratozoospermia, and normozoospermic fertile controls. Data were pooled using a random-effects model to calculate the standardized mean difference (SMD) with 95% confidence intervals (CIs). Heterogeneity was assessed using the I² statistic. Results: Seven case-control studies comprising 658 infertile men and 612 normozoospermic controls met the inclusion criteria. The quality of the included studies was moderate to high. The pooled data revealed that sperm mtDNAcn was significantly higher in infertile men compared to fertile controls (SMD = 1.28, 95% CI: 0.81 to 1.75, p < 0.00001). Significant heterogeneity was observed among the studies (I² = 88%, p<0.00001). Subgroup analysis based on infertility phenotype showed a consistently elevated mtDNAcn across oligozoospermia, asthenozoospermia, and oligoasthenoteratozoospermia (OATs). The funnel plot was largely symmetrical, and Egger’s test showed no significant evidence of publication bias (p = 0.12). Conclusion: This meta-analysis provides strong evidence that an elevated sperm mtDNA copy number is significantly associated with male infertility. This quantitative alteration may serve as a crucial biomarker for assessing sperm quality and spermatogenic dysfunction. The findings suggest that increased mtDNAcn likely represents a compensatory response to underlying mitochondrial defects and oxidative stress, warranting further investigation into its clinical utility for diagnosing and managing male infertility.

The Predictive Value of Sperm Mitochondrial DNA Copy Number on Male Infertility: A Systematic Review and Meta-Analysis Aghnia Yuslicha Yuniar; Dwi Ari Pujianto
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 8 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i8.1362

Background: The integrity and quantity of mitochondrial DNA (mtDNA) in spermatozoa are considered critical for male reproductive potential. While qualitative damage like deletions has been linked to infertility, the significance of quantitative changes, specifically mtDNA copy number (mtDNAcn), remains debated. This study aimed to systematically review and meta-analyze the existing evidence to determine the association between sperm mtDNAcn and male infertility. Methods: We conducted a systematic search of PubMed, Science Direct, and Scopus databases for observational studies published between January 2014 and December 2024. The search included studies that compared sperm mtDNAcn between infertile men, who were diagnosed with conditions such as oligozoospermia, asthenozoospermia, and teratozoospermia, and normozoospermic fertile controls. Data were pooled using a random-effects model to calculate the standardized mean difference (SMD) with 95% confidence intervals (CIs). Heterogeneity was assessed using the I² statistic. Results: Seven case-control studies comprising 658 infertile men and 612 normozoospermic controls met the inclusion criteria. The quality of the included studies was moderate to high. The pooled data revealed that sperm mtDNAcn was significantly higher in infertile men compared to fertile controls (SMD = 1.28, 95% CI: 0.81 to 1.75, p < 0.00001). Significant heterogeneity was observed among the studies (I² = 88%, p<0.00001). Subgroup analysis based on infertility phenotype showed a consistently elevated mtDNAcn across oligozoospermia, asthenozoospermia, and oligoasthenoteratozoospermia (OATs). The funnel plot was largely symmetrical, and Egger’s test showed no significant evidence of publication bias (p = 0.12). Conclusion: This meta-analysis provides strong evidence that an elevated sperm mtDNA copy number is significantly associated with male infertility. This quantitative alteration may serve as a crucial biomarker for assessing sperm quality and spermatogenic dysfunction. The findings suggest that increased mtDNAcn likely represents a compensatory response to underlying mitochondrial defects and oxidative stress, warranting further investigation into its clinical utility for diagnosing and managing male infertility.

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