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POTENCY OF BIOCONTROL AGENTS ISOLATED FROM COMPOST AND PEAT SOIL OF TROPICAL PEAT SWAMP FOREST IN KALAMPANGAN ZONE, CENTRAL KALIMANTAN Yuliar, Yuliar; Abidin, Zaenal; Mangunwardoyo, Wibowo
Indonesian Journal of Forestry Research Vol 8, No 2 (2011): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

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Abstract

Rhizoctonia solani is a soil pathogen that causes diseases in wide range of hosts of agricultural, horticultural and flower crops. Biological control is the most promising way for the diseases management and it is environment friendly too. The objective of this study was to isolate and screen the potency of soil bacteria as biological control from various local compost and peat soil of tropical peat swamp forest in Kalampangan Zone, Central Kalimantan. Forty seven isolates from peat soil and compost were screened for biocontrol agent of Rhizoctonia solani . R. Solani Seven out of thirteen peat soil isolates, and six out of thirty three compost isolates showed antagonistic activity against in Potato Dextrose Agar. The cultivation of the antagonistic isolates in Trypticase Soy Broth (TSB) was extracted and analysed by high performance liquid chromatography (HPLC) column. The HPLC analyzes indicated that the antagonistic isolates produce an antifungal iturin A. Macroscopic observation of isolates colonies showed that form of their colonies were amuboid, myceloid, curled, circular, rhizoid, irregular and filamentous. These achievement indicate peat swamp forest not only offer a potential biocontrol agents of damping off but also provide a new source for production of antibiotics.
POTENCY OF BIOCONTROL AGENTS ISOLATED FROM COMPOST AND PEAT SOIL OF TROPICAL PEAT SWAMP FOREST IN KALAMPANGAN ZONE, CENTRAL KALIMANTAN Yuliar, Yuliar; Abidin, Zaenal; Mangunwardoyo, Wibowo
Indonesian Journal of Forestry Research Vol 8, No 2 (2011): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2011.8.2.144-157

Abstract

Rhizoctonia solani is a soil pathogen that causes diseases in wide range of hosts of agricultural, horticultural and flower crops. Biological control is the most promising way for the diseases management and it is environment friendly too. The objective of this study was to isolate and screen the potency of soil bacteria as biological control from various local compost and peat soil of tropical peat swamp forest in Kalampangan Zone, Central Kalimantan. Forty seven isolates from peat soil and compost were screened for biocontrol agent of Rhizoctonia solani . R. Solani Seven out of thirteen peat soil isolates, and six out of thirty three compost isolates showed antagonistic activity against in Potato Dextrose Agar. The cultivation of the antagonistic isolates in Trypticase Soy Broth (TSB) was extracted and analysed by high performance liquid chromatography (HPLC) column. The HPLC analyzes indicated that the antagonistic isolates produce an antifungal iturin A. Macroscopic observation of isolates colonies showed that form of their colonies were amuboid, myceloid, curled, circular, rhizoid, irregular and filamentous. These achievement indicate peat swamp forest not only offer a potential biocontrol agents of damping off but also provide a new source for production of antibiotics.
PENGARUH KONSENTRASI MEDIA SOYBEAN DAN 20 JENIS L- ASAM AMINO PADA PRODUKSI ANTI JAMUR, ITURIN A Yuliar, Yuliar
Jurnal Teknologi Lingkungan Vol. 10 No. 3 (2009)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.361 KB) | DOI: 10.29122/jtl.v10i3.1475

Abstract

Influence of soybean meal concentration and 20 kinds of L-amino acids in iturin A production. Theuse of chemical pesticides has caused serious environmental problems and thus the demandfor safer pesticides is increasing. One alternative is microbial pesticides that suppress fungaland bacterial of plant pathogens. Bacillus subtilis has been known as producer of lipopeptideantibiotics, like iturin A, plipastatin and surfactin. In this study, iturin A as an antifungal of planpathogens was produced on varieties of soybean meal concentration; i.e. 8%, 10%, 12%, and15% using B.subtilis RB14-CS.The result indicates that 8% soybean meal concentration produced the highest of iturin Aproduction ( 2469 mg L-1) compared to the others. Beside the effect of nitrogen source (soybean), the influence of 20 kinds of L-amino acids on an enhancement of iturin A productivitywere observed. The examined L-amino acids were L-ala, L-arg, L-asn, L-asp, L-cys, L-glu, L-gln,L-gly, L-his, L-ile, L-leu, L-lys, L-met, L-pro, L-phe, L-ser, L-thr, L-tyr, L-trp and L-val. The resultsshow that no one of them could improve the iturin A productivity on soybean meal medium.
ISOLASI, IDENTIFIKASI, DAN SKRINING JAMUR ENDOFIT PENGHASIL AGEN BIOKONTROL DARI TANAMAN DI LAHAN PERTANIAN DAN HUTAN PENUNJANG GUNUNG SALAK Suciatmih, Suaciatmih; yuliar, Yuliar; Supriyati, D
Jurnal Teknologi Lingkungan Vol. 12 No. 2 (2011)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.987 KB) | DOI: 10.29122/jtl.v12i2.1249

Abstract

Isolation of endophytic fungi was done to find alternative microorganisms as biocontrol agents against Rhizoctonia solani Kuhn, a soil borne pathogen on many agriculturalcrops. The research objectives were 1) to isolate and identify endophytic fungi colonize plants growing on agricultural and Mount Salak supporting forest lands in the Villages of Parakan Salak and Cimalati, Sukabumi; and 2) to screen for their biocontrol agent activity againt R. solani. Diameter of R. solani was measured on day 2 after inoculation, and its percent inhibition of growth by endophytic fungi is calculated using the formula Skidmore & Dickinson (1976). The results indicated that 214 isolates of endophytic fungi were isolated from 96 plant species that growing in both places. Endophytic fungi isolated including in the group of Aspergillus, Cladosporium, Colletotrichum, Curvularia, Drechslera, Fusarium, Guignardia, Mucor, Nigrospora, Paecilomyces, Penicillium, Pestalotiopsis, Phoma, Phomopsis, Rhizoctonia, and endophytic fungi that have not been identified. Of the 214 fungal isolates tested, 39 isolates (18.22 %) could inhibit the growth of R. solani from 10.18 % to 58.99 % with a percent inhibition. The highest growth inhibition of R. solani were shown respectively by an unidentified fungus isolatedfrom Hyptis capitata Jack (58.99%), Cladosporium sp. isolated from jeruk bali (Citrus grandis (L.) Osbeck) (55.42%), Pestalotiopsis sp. isolated from pine apple (Ananas comosus (L.) Merr.) (53.85 %), and Paecilomyces lilacinus (Thom) Samson isolated from banyan (Ficus benyamina L.) (51.81%) 
Analisis Efek Konsentrasi Ekstrak Etanol Daun Senggani (Melastoma malabathricum L.) sebagai Antibakteri Staphylococcus aureus Nurhayat, Nurhayat; Yuliar, Yuliar; Marpaung, Mauritz Pandapotan
JURNAL KESEHATAN POLTEKKES KEMENKES RI PANGKALPINANG Vol 8, No 1 (2020): JKP Juni 2020
Publisher : Poltekkes Kemenkes Pangkalpinang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32922/jkp.v8i1.115

Abstract

Latar belakang: Tanaman Senggani mempunyai banyak potensi sebagai pengobatan seperti antipiretik, analgesik, diuretik, dan antibakteri. Tujuan:untuk menganalisis efek konsentrasi ekstrak etanol daun senggani sebagai antibakteri Staphylococcus aureus. Metode: Daun senggani diekstraksi dengan perendaman dalam etanol 96%. Diameter zona hambat bakteri diukur dengan menggunakan metode difusi agar melalui lima perlakuan yaitu kontrol positif (kloramfenikol), kontrol negatif (NaCMC), dan ekstrak daun senggani konsentrasi 20%, 40%, 60%, dan 80%.Data yang dihasilkan dianalisis dengan metode statistik Kruskal-Wallis dan uji lanjut post hoc Mann Whitney. Hasil: Rata-rata diameter zona hambat pertumbuhan bakteri yang dihasilkan pada ekstrak 20% sebesar 12,5±1,00 mm; ekstrak 40% sebesar 14,75±0,96 mm; ekstrak 60% sebesar 17±0,82 mm; dan ekstrak 80% sebesar 19,5±1,00 mm.Hasil uji Kruskal-Wallis menunjukkan ada pengaruh antara rata-rata diameter zona hambat pertumbuhan bakteri Staphylococcus aureus dengan kelompok larutan uji (p
Potential Antagonists Trichoderma viride as Biofungicide, Plant Spacing, and Agricultural Lime Application to Suppress Anthracnose on Chili Tasrif, Arifin; Yuliar, Yuliar; Sulistyowati, Dwiwanti; Krisnawati, Endang; Adirianto, Bayu; Sugiharti, Dwi
Jurnal Perlindungan Tanaman Indonesia Vol 28, No 1 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.87342

Abstract

Anthracnose caused by Colletotrichum capsici and C. gloesporium on chili is a disease that can reduce chili yields up to 80%. Control with fungicide has not been able to provide maximum results, because Colletotrichum can spread due to splashing of water, especially in the rainy season. The use of antagonistic fungi against Colletotrichum spp. has been widely published but is still limited to the laboratory and greenhouse scale, while field conditions are unpredictable. This study aims to identify the potency of Trichoderma viride that can be used as a biofungicide to control anthracnose in chili and to determine aspects of agronomic that can reduce the risk of anthracnose in chili. Samples of infected plants of stems, leaves, and fruits were collected from experimental farm of Bogor Agricultural Development Polytechnic. The experiment was conducted using Randomized Complete Design and Randomized Complete Block Design. The percentage of disease intensity of the fungus C capsici and the intensity of anthracnose in chili both under screen house and open fields. While testing the effect of cultivation aspects using different types of fertilizer on plant height, fruit weight, number of fruits, and the percentage of disease intensity of Colletotrichum spp. were analyzed. The results of this study shown that four isolates of fungi have been identified such as Penicillium sp., Aspergillus flavus, T. viride, and C. capsici. In vitro analysis shown the ability of T. viride to suppress the growth of the C. capsici  up to 71%. The fungus T. viride with a density of 7×106 CFU/mL can suppress the development of anthracnose by 59 to 87% under screen house conditions. However, under field conditions, the fungus T. viride was not able to suppress the development of anthracnose. Agronomic aspects such as plant height, number of fruits and production, and productivity of chili were not significantly effect on anthracnose.
HUBUNGAN MUTU PELAYANAN KEPERAWATAN DENGAN KEPUASAAN PASIEN DI RUANG RAWAT INAP RSUD SITI FATIMAH Yuliar, Yuliar; Sumarni, Titin; Latip, Abdul
Journal Safety and Healthty Vol 1 No 1 (2024): Journal Of Safety and Health
Publisher : Program Studi Magister Kesehatan Masyarakat, Universitas Kader Bangsa Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.461 KB) | DOI: 10.54816/josh.v1i1.361

Abstract

Patient satisfaction is still a problem in several hospitals in Indonesia and even the world. This study aims to determine the relationship between service quality and patient satisfaction in the Inpatient Room of Siti Fatimah Hospital, South Sumatra Province in 2019. This type of research used cross sectional. Statistical test using chi-square test using accidental sampling with a total of 50 respondents. The analysis was carried out in two stages, namely Univariate and Bivariate analysis, the results showed that the quality of nursing services at Siti Fatimah Hospital was good. Patient satisfaction in Siti Fatimah Hospital is high. There is a relationship between the quality of nursing services with patient satisfaction in Siti Fatimah Hospital. The better the quality of nursing services provided, the higher the level of patient satisfaction with the services provided. This research is expected to be a consideration for the leadership of Siti Fatimah Hospital in improving the quality of good service to patients so that the level of patient satisfaction is high.
HUBUNGAN MELATIH ANAK, MOTIVASI ORANG TUA, KESIAPAN ANAK TERHADAP KEMAMPUAN TOILET TRAINING PADA ANAK USIA TODDLER DI WILAYAH KERJA PUSKESMAS MEKARSARI KABUPATEN BANYUASIN Yuliar, Yuliar; Sari, Eliya Pitri; Feriliana, Feriliana
Journal Safety and Healthty Vol 1 No 2 (2024): Journal Of Safety and Health
Publisher : Program Studi Magister Kesehatan Masyarakat, Universitas Kader Bangsa Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.71 KB)

Abstract

Salah satu tugas keluarga terhadap pertumbuhan dan perkembangan anak adalah membentuk kemandirian. Peran aktif orang tua terhadap perkembangan anak adalah berada pada fase anal (1-3 tahun) dimana pada tahap ini daerah yang sensitif untuk memperoleh kenikmatan adalah pada daerah anus dan pada proses menahan juga pengeluaran kotoran. Pada masa ini orang tua harus mulai melatih kemampuan anaknya untuk buang air kecil dan buang air besar ke toilet. Penelitian ini bertujuan untuk mengetahui hubungan melatih anak, motivasi orang tua dan kesiapan toilet dengan kemampuan toilet todler. Jenis penelitian ini menggunakan cross sectional. Uji statistik menggunakan uji chi-square dengan menggunakan accidental sampling dengan jumlah 50 responden. Analisis dilakukan dalam dua tahap yaitu analisis Univariat dan Bivariat. Didapatkan hasil bahwa Penelitian disimpulkan bahwa Ada hubungan antara melatih anak terhadap kemampuan toilet training pada anak usia toddler nilai p value = 0,028 yang lebih kecil dari 0,05. Ada hubungan antara motivasi orang tua terhadap kemampuan toilet training pada anak usia toddler nilai p value = 0,000 yang lebih kecil dari 0,05. Ada hubungan antara kesiapan anak terhadap kemampuan toilet training pada anak usia toddler nilai p value = 0,043 ang lebih kecil dari 0,05. Di harapkan orang tua dapat membantu atau mengingatkan kepada anak sejak dini tentang toilet training tidak hanya memberikan instruksi tetapi juga mengarahkan secara langsung yang benar agar mudah dipraktekkan langsung oleh anak.