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Journal : JURNAL TEKNOLOGI LINGKUNGAN

PENGARUH KONSENTRASI MEDIA SOYBEAN DAN 20 JENIS L- ASAM AMINO PADA PRODUKSI ANTI JAMUR, ITURIN A Yuliar, Yuliar
Jurnal Teknologi Lingkungan Vol. 10 No. 3 (2009)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.361 KB) | DOI: 10.29122/jtl.v10i3.1475

Abstract

Influence of soybean meal concentration and 20 kinds of L-amino acids in iturin A production. Theuse of chemical pesticides has caused serious environmental problems and thus the demandfor safer pesticides is increasing. One alternative is microbial pesticides that suppress fungaland bacterial of plant pathogens. Bacillus subtilis has been known as producer of lipopeptideantibiotics, like iturin A, plipastatin and surfactin. In this study, iturin A as an antifungal of planpathogens was produced on varieties of soybean meal concentration; i.e. 8%, 10%, 12%, and15% using B.subtilis RB14-CS.The result indicates that 8% soybean meal concentration produced the highest of iturin Aproduction ( 2469 mg L-1) compared to the others. Beside the effect of nitrogen source (soybean), the influence of 20 kinds of L-amino acids on an enhancement of iturin A productivitywere observed. The examined L-amino acids were L-ala, L-arg, L-asn, L-asp, L-cys, L-glu, L-gln,L-gly, L-his, L-ile, L-leu, L-lys, L-met, L-pro, L-phe, L-ser, L-thr, L-tyr, L-trp and L-val. The resultsshow that no one of them could improve the iturin A productivity on soybean meal medium.
ISOLASI, IDENTIFIKASI, DAN SKRINING JAMUR ENDOFIT PENGHASIL AGEN BIOKONTROL DARI TANAMAN DI LAHAN PERTANIAN DAN HUTAN PENUNJANG GUNUNG SALAK Suciatmih, Suaciatmih; yuliar, Yuliar; Supriyati, D
Jurnal Teknologi Lingkungan Vol. 12 No. 2 (2011)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.987 KB) | DOI: 10.29122/jtl.v12i2.1249

Abstract

Isolation of endophytic fungi was done to find alternative microorganisms as biocontrol agents against Rhizoctonia solani Kuhn, a soil borne pathogen on many agriculturalcrops. The research objectives were 1) to isolate and identify endophytic fungi colonize plants growing on agricultural and Mount Salak supporting forest lands in the Villages of Parakan Salak and Cimalati, Sukabumi; and 2) to screen for their biocontrol agent activity againt R. solani. Diameter of R. solani was measured on day 2 after inoculation, and its percent inhibition of growth by endophytic fungi is calculated using the formula Skidmore & Dickinson (1976). The results indicated that 214 isolates of endophytic fungi were isolated from 96 plant species that growing in both places. Endophytic fungi isolated including in the group of Aspergillus, Cladosporium, Colletotrichum, Curvularia, Drechslera, Fusarium, Guignardia, Mucor, Nigrospora, Paecilomyces, Penicillium, Pestalotiopsis, Phoma, Phomopsis, Rhizoctonia, and endophytic fungi that have not been identified. Of the 214 fungal isolates tested, 39 isolates (18.22 %) could inhibit the growth of R. solani from 10.18 % to 58.99 % with a percent inhibition. The highest growth inhibition of R. solani were shown respectively by an unidentified fungus isolatedfrom Hyptis capitata Jack (58.99%), Cladosporium sp. isolated from jeruk bali (Citrus grandis (L.) Osbeck) (55.42%), Pestalotiopsis sp. isolated from pine apple (Ananas comosus (L.) Merr.) (53.85 %), and Paecilomyces lilacinus (Thom) Samson isolated from banyan (Ficus benyamina L.) (51.81%)ÂÂ