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Genetic Variation of Agathis loranthifolia Salisb. in West Jawa Assessed by RAPD Yunanto, Tedi; Djamhuri, Edje; Siregar, Iskandar Z.; Ulfah, Mariyana
JURNAL BIOLOGI INDONESIA Vol 7, No 1 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i1.3125

ABSTRAKVariasi Genetika Agathis loranthifolia Salisb Jawa Barat Menggunakan Analisis RAPD.Agathis loranthifolia Salisb merupakan salah satu tumbuhan hutan yang menghasilkan hasilhutan bukan berupa kayu yaitu berupa getah. Untuk mengetahui derajad variasi genetika darijenis ini maka dicoba dianalisis menggunakan RAPD. Sampel daun tumbuhan ini di perolehdari Perum Perhutani Cianjur dan Garut. Variasi genetika populasi tumbuhan ini dari Cianjursebesar He = 0.1952 atau lebih tinggi dari populasi asal Garut (He = 0.1125). Namun berdasarkanproduksi copalnya menunjukkan bahwa variasi genetika pohon Lanang paling tinggi He =0.2105Kata kunci: RAPD, kopal, Agathis loranthifolia

GENETIC VARIATION OF AGATHIS LORANTHIFOLIA SALISB. IN WEST JAWA ASSESSED BY RAPD Yunanto, Tedi; Djamhuri, Edje; Siregar, Iskandar Z.; Ulfah, Mariyana
JURNAL BIOLOGI INDONESIA Vol 7, No 1 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i1.3125

ABSTRAKVariasi Genetika Agathis loranthifolia Salisb Jawa Barat Menggunakan Analisis RAPD.Agathis loranthifolia Salisb merupakan salah satu tumbuhan hutan yang menghasilkan hasilhutan bukan berupa kayu yaitu berupa getah. Untuk mengetahui derajad variasi genetika darijenis ini maka dicoba dianalisis menggunakan RAPD. Sampel daun tumbuhan ini di perolehdari Perum Perhutani Cianjur dan Garut. Variasi genetika populasi tumbuhan ini dari Cianjursebesar He = 0.1952 atau lebih tinggi dari populasi asal Garut (He = 0.1125). Namun berdasarkanproduksi copalnya menunjukkan bahwa variasi genetika pohon Lanang paling tinggi He =0.2105Kata kunci: RAPD, kopal, Agathis loranthifolia

Inference on the Possible Causes of Segregation Distortion from Open Pollination Progenies of Merkus Pine (Pinus merkusii) ISKANDAR ZULKARNAEN SIREGAR; TEDI YUNANTO
HAYATI Journal of Biosciences Vol. 15 No. 4 (2008): December 2008
Publisher : Bogor Agricultural University, Indonesia

Methods of analysis to infer the possible causes of segregation distortion were carried out using single tree progenies of Pinus merkusii. Tests on the progenies of the seed trees showing significant segregation distortion were performed at six gene loci, i.e. GOT-C, GOT-D, PGM-B, SKDH-A, NDH-A, and FDH-A. Results indicated that most fertilisation combination between female and male gametes occurred randomly. This study revealed that 11 out of 17 cases of distortion were detected in both gametes, while the other six were detected only in female gamet. The primary sources of such segregation distortions are discussed regarding to linkage relationships system of mating and post-zygotic viability selection. Key words: Pinus merkusii, segregation distortion, system of mating, viability selection

Pengembangan metode penanda genetika molekuler untuk lacak balak (studi kasus pada jati) Iskandar Z. Siregar; Ulfah J. Siregar; Lina Karlinasari; Tedi Yunanto
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 2 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.425 KB)

Tracking timber on teak and teak wood products can be conducted using different methods, such as DNA finger printing, chemical composition of the wood, Near Infra Red spectra (NIR) and stable isotopes. Samples were collected from wood material and leaves in Java (9 Forest Management Units district) of Perhutani to determine: i) pattern of genetic variation within and among populations, ii) to determine the protocol for DNA extraction from wood, and iii) to study the feasibility of DNA marker for timber tracking in the field. Results show that: i) genetic variation of cpDNA (PCR-RFLP) is low, while RAPD variation is moderate, ii) there are differences in chemical composition of wood among the Forest Management Units (FMUs) of Perhutani, iii) variation of isotopic carbon and oxygen in Central and East Java were higher than from of West Java, iv) absorbtion intensity of NIR in West Java was higher than for Central and East Java, and iv) testing of DNA marker showed that genetic structure in the forest site is not significantly different from that in log yards, indicating that the timber flow is still according to the procedure. RAPD marker also is able to determine the origin of illegal timber and wood in industry without clear identity. Keywords: genetik marker, teak, timber tracking

Aplikasi Isotop δ13C dan δ18O untuk Lacak Balak Kayu Jati (Tectona grandis Linn.f.) di Jawa Application of Isotopes δ13C and δ18O for Teak Wood (Tectona grandis Linn.f.) Timber Tracking in Java Agus Kholik; Djiono Djiono; Iskandar Z Siregar; Ulfah J Siregar; Lina Karlinasari; Tedi Yunanto
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 7, No 1 (2009): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Tracking the origin of Teak timber and its wood product is possible to be carried out using stable isotopes fingerprinting. To enrich the available variation patterns, Teak woods samples were collected from nine Forest Management Units (FMUs) representing all provinces and Teak centres in Java. Mills from all part of woods were mixed for analyses. The mills were burned under vacuum in a reactor system at 1200oC for 2 h, the resulting clean CO2 was transferred to the mass spectrometer to determine its isotopic composition of δ13C and δ18O. The generated data was then analysed using multivariate analysis. The results showed that isotopes analysis grouped Teak populations in Java intoWest Java population and mixed Central-East Java population. To consider wood as material for fingerprinting traded forest product, a refinement on method should be done.

Vegetation development and soil conditions in reclaimed areas of former silica mines in Indonesia Yunanto, Tedi; Wildan, Dadan; Suparno; Megawati; Santoso, Natasya
Journal of Degraded and Mining Lands Management Vol. 12 No. 2 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.122.7141

To restore the function of ex-mining land according to its designation, the ex-mining area must be reclaimed. Ecological dynamics, especially soil conditions and the composition, structure, and biodiversity that occur in reclamation areas, need to be understood so that the reclamation area meets the criteria for the success of mine reclamation. This study aimed to understand the composition, structure, biodiversity, and soil conditions that influence growth in the reclamation area. The research was conducted in a silica mine reclamation area with a 17.8 m radius (r) circular plot (0.1 ha) of 10 plots (±1 ha) for vegetation and 9 points for soil sampling at a depth of 0-30 cm and 30-60 cm. In general, the results of the vegetation inventory show that the tree species recorded are dominated by Pinus merkusii, Hevea brasiliensis, and Enterolobium cyclocarpum with mean values of diameter, height, and low diversity conditions. The condition of soil physical and chemical properties in the reclamation area at both depths shows low mean values, which can affect the growth rate of plants in the reclamation area. The species of Schima wallichii that grow naturally in the reclamation area was found, indicating that the reclamation activities have created a good environment for other tree species to grow naturally. However, enrichment activities need to be carried out to increase biodiversity.

Struktur Tegakan dan Tingkat Keberhasilan Area Reklamasi pada Tambang Silika Tedi Yunanto; Dadan Wldan; Suparno
Journal PEP Bandung Vol 1 No 1 (2024): Applied Geo-Mining and Metallurgy
Publisher : PEP Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65706/gm.v1i1.31

Sebagai negara berkembang, Indonesia masih bergantung terhadap eksploitasi sumber daya alam untuk sumber pendapatan nasional, seperti komoditas tambang Silika di Kabupaten Sukabumi. Untuk mengembalikan fungsi lahan bekas tambang sesuai peruntukannya, maka area bekas tambang wajib dilakukan kegiatan reklamasi. Dinamika ekologi khususnya struktur tegakan terjadi pada area reklamasi dan area reklamasi tersebut harus memenuhi kriteria keberhasilan berdasarkan peraturan perundangan yang berlaku. Tujuan penelitian adalah untuk memahami dinamika ekologi pada area reklamasi bekas tambang silica dan mengukur tingkat keberhasilan reklamasi sesuai peraturan perundangan. Penelitian dilaksanakan pada area reklamasi tambang silika dengan plot lingkaran jari-jari (r) 17,8 m (0,1 ha) sebanyak 10 plot (±1 ha). Secara umum, rata-rata diameter pohon di areal reklamasi pada semua plot adalah 18,70 cm (aritmatika) dan 18,99 cm (kuadrat). Grafik yang dibentuk dari hubungan jumlah pohon dengan kelas diameter dibagi menjadi tiga bentuk, yaitu J-shape terbalik, kurva sebaran normal yang tidak sempurna dan kurva sebaran normal yang sempurna. Rata-rata tinggi total untuk semua plot adalah 11.87 m untuk aritmatika dan 12.25 m untuk Lorey’s. Hasil sampling dari 10 plot lingkaran (1 ha) didapatkan bahwa area reklamasi tersebut mencapai tingkat keberhasilan 96,40%. Beberapa parameter kriteria keberhasilan yang belum terpenuhi diantaranya adalah jumlah tanaman, penutupan cover crop dan tajuk. Terdapat jenis puspa (Schima wallichii) yang tumbuh alami pada area reklamasi dan dapat ditemukan pada plot 2, 5, 6, 7 dan 8. Untuk meningkat keanekaragaman perlu dilakukan proses pengayaan, yaitu menanam jenis tanaman lainnya.

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