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Widodo Widodo
IPB University
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Kemampuan Kolonisasi Cendawan Endofit dan Peningkatan Pertumbuhan Bibit Cabai Evan Purnama Ramdan; Efi Toding Tondok; Suryo Wiyono; Sri Hendrastuti Hidayat; Widodo Widodo
Proceedings Series on Physical & Formal Sciences Vol. 2 (2021): Prosiding Seminar Nasional Fakultas Pertanian dan Perikanan
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (395.798 KB) | DOI: 10.30595/pspfs.v2i.175

Abstract

Endophytic fungi are fungi that live in healthy plant tissues without causing disease symptoms. Endophytic fungi in chili have been tested as both biocontrol agents and growth promoters, but their colonization has not been reported. Therefore, this study aimed to determine the level of colonization of endophytic fungi and its effect on the growth of chili seedlings. A total of 8 endophytic fungi were prepared at a density of 2.8 × 106 CFU mL-1. Then the endophytic fungus was inoculated 2 times, first by soaking the seeds, and secondly by watering the endophytic fungus suspension on chili seedlings aged 3 weeks after sowing. Endophytic fungi were re-isolated on chili seedlings that were 4 weeks old after sowing on the roots and stems to determine their colonization ability. Chili seeds were then maintained for up to 4 weeks after transplanting to observe their growth. The results showed that the endophytic fungal colonization ranged from 26-60% on the chili root, while at the base of the stem it was 20-40% with a different pattern of colonization distribution. In addition, endophytic fungus colonization was also able to increase the shoot height and root length of chili seedlings.
Peran Solarisasi Tanah terhadap Pertumbuhan Patogen Tular Tanah dan Populasi Mikroba Tanah Evan Purnama Ramdan; Astri Afriani; Andini Hanif; Cheppy Wati; Nurholis Nurholis; Dwi Astuti; Widodo Widodo
Agrotechnology Research Journal Vol 6, No 1 (2022): Agrotechnology Research Journal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (331.97 KB) | DOI: 10.20961/agrotechresj.v6i1.55979

Abstract

Soil-borne soil pathogens are pathogens that inhabit the soil and can survive for years in the soil, making them very difficult to control. Control with pesticides and fungicides harms the ecosystem, so other controls are needed such as soil solarization. This study aims to determine the growth response of soil-borne pathogens and soil microbial populations to soil solarization treatment. The study used a completely randomized design with 4 treatments consisting of solarization on soil media, solarization on soil and compost media, without solarization on soil media, and without solarization on soil media and compost on plastic trays. Each treatment was repeated 4 times. The soil-borne pathogens used were Sclerotium rolfsii and Rigidoporus lignisus. Each pathogen was planted in each planting medium with a depth of 5 and 10 cm. Then each tray is covered with 0.1 mm thick transparent plastic. Then given solarization treatment for four weeks. At the end of the observation, sclerotia and R. lignosus were grown on PDA media to be tested for pathogen survival and the solarization efficacy against pathogen growth was calculated. Soil samples from each treatment were also taken to calculate the soil microbial population. The results showed that soil solarization was able to suppress the growth of R. lignosus by 80-100% and S. rolfsii by 100%. Meanwhile, the microbes found in the soil solarization treatment consisted of groups of bacteria and fungi, respectively 7.67×104–1.90×107 CFU.mL-1 and 1.00×104–5.82×105 CFU.mL-1.
Co-Authors Afriani, Astri Andini Hanif Dwi Astuti Efi Toding Tondok Nurholis Nurholis Ramdan, Evan Purnama SRI HENDRASTUTI HIDAYAT Suryo Wiyono Wati, Cheppy