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All Journal Jurnal Fitopatologi Indonesia JURNAL ILMU PERTANIAN KUMPULAN JURNAL DOSEN UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA Hasil Pengabdian Kepada Masyarakat Agrotechnology Research Journal Agro Bali: Agricultural Journal PERTANIAN TROPIK

Andini Hanif

Universitas Muhammadiyah Sumatera Utara

Author-ID : 702617

Agriculture, Biological Sciences & Forestry Computer Science & IT Education Engineering Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology

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Seleksi Bakteri Endofit Penghasil Senyawa Metabolit untuk Pengendalian Cendawan Patogen Terbawa Benih Jagung Andini Hanif; Bonny Poernomo Wahyu Soekarno; Abdul Munif
Jurnal Fitopatologi Indonesia Vol 12 No 5 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Endophytic bacteria have been reported to produce metabolite as antifungal compound. This study was aimed to obtain endophytic bacteria which are able to produce metabolite to control Fusarium sp., a potential seedborne fungi on maize. Endophytic bacteria were screened by hypersensitive test on tobacco leaves and antagonistic test. Endophytic bacteria isolates with high growth inhibitor activity were selected and examined for their metabolite compound. Thre isolates, i.e. Lactobacillus sp. isolate EF14III, Pseudomonas sp. isolate ER1I, dan Aeromonas sp. isolate ER10I has the potential to inhibit Fusarium sp.. Metabolite compound of Pseudomonas sp. isolates ER1I was able to decrease the infection Fusarium sp. by 65.0% in blotter test and decreased infection of Fusarium sp. up to 59.5% and 60.5% in growing on test using water agar and sterile soil, respectively. Cyclohexanone with concentration of 9.68% produced by Pseudomonas sp. isolat ERI1 may play a role as antifungal compound.

Analisa Kadar Kualitatif Senyawa Lutein dari Tanaman Kenikir (Tagetes erecta L) Sebagai Mikrohabitat Dari Musuh Alami Hama. Rini Susanti; Andini Hanif; Lisdayani Lisdayani
AGRIUM: Jurnal Ilmu Pertanian Vol 21, No 3 (2018)
Publisher : UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA

Tagetes erecta merupakan tanaman yang tumbuh liar dan mempunyai mahkota bunga berwarna oranye, mempunyai manfaat sebagai agen kemopreventif karena mempunyai kandungan senyawa-senyawa bersifat antioksidan untuk menangkal radikal bebas. Manfaat lain dari bunga Tagetes erecta dapat berfungsi sebagai refugia mikrohabitat bagi beberapa jenis serangga musuh alami yang bisa menarik serangga musuh alami karena mempunyai bunga yang berwarna cerah . Penelitian bertujuan untuk melihat kadar kuantitatif dari bunga kenikir (Tagetes erecta) sehingga dapat berfungsi sebagai mikrohabitat bagi beberapa jenis musuh alami yang ada disekitar areal pertanaman petani di desa patumbak. Metode penelitian menggunakan analisa deskriptif dengan cara analisa kuantitatif kadar lutein dari bunga kenikir yang dilakukan di Laboratorium pertanian Universitas Muhammadiyah Sumatera Utara. Hasil penelitian didapat kadar kuantittif dari ekrtak T.erecta didapat kadar lutein crude sebesar 4.33 mg dan KLT dengan Rf sebesar 0.57 cm

UJI ANTAGONIS BAKTERI ENDOFIT ASAL TANAMAN JAGUNG TERHADAP Fusarium sp. PENYEBAB PENYAKIT LAYU FUSARIUM Andini Hanif
KUMPULAN JURNAL DOSEN UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA Jurnal PADI
Publisher : KUMPULAN JURNAL DOSEN UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (655.924 KB)

Bakteri endofit dilaporkan mampu menghasilkan senyawa metabolit yang berpotensi memiliki senyawa anticendawan. Tujuan dari penelitian ini adalah untuk mendapatkan bakteri endofit asal tanaman jagung yang antagonis terhadap cendawan patogen Fusarium sp. Isolasi bakteri endofit dilakukan dengan metode sterilisasi permukaan, selanjutnya dilakukan uji hipersensitif terhadap isolat bakteri yang diperoleh untuk menyeleksi bakteri patogen dan non patogen. Isolat bakteri endofit diuji kemampuan antagonisnya terhadap cendawan Fusarium sp. Dengan metode dual culture. Hasil penelitian diperoleh 31 isolat bakteri endofit non patogen. Uji antagonis menunjukkan tiga bakteri endofit berpotensi menghambat cendawan Fusarium sp. yaitu isolat EF14III dengan daya hambat sebesar 64.4%, ER1I dengan daya hambat sebesar 58.0%, dan ER10I dengan daya hambat sebesar 56.4%. Berdasarkan karakterisasi secara morfologi, biokimia, dan fisiologi isolat EF14III diidentifikasi sebagai Lactobacillus sp., ER1I diidentifikasi sebagai Pseudomonas sp., dan ER10I diidentifikasi sebagai Aeromonas sp.

ANALISIS SENYAWA ANTIFUNGAL BAKTERI ENDOFIT ASAL TANAMAN JAGUNG (Zea Mays L.) Andini Hanif; Rini Susanti
KUMPULAN JURNAL DOSEN UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA Vol 2, No 01 (2017): JASA PADI
Publisher : KUMPULAN JURNAL DOSEN UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.816 KB)

AbstracEndophytic bacteria repoted can produce metabolite as antifungal compound. This study was aimed to knowing and obtain antifungal compound wich are produced of endophytic bacteria to control fungal pathogen. Isolation of endophytic bacteria was done using surface sterilization method, furthermore hypersensitive reaction test of bacteria was done for selection pathogenicity of bacteria. Endophytic bacteria was tested their antagonism against fungal pathogen for selection the potential of endophytic bacteria. Isolate of endophytic bacteria which have highest growth inhibitor would be extracted and analyzed the antifungal compound. The result showed that threeisolate of endophytic bacteria wich one potentially in inhibiting fungal pathogen are isolate EF14III, ER1I and ER101. Isolate bacteria ER1I was the most effective in inhibiting the growth of fungal pathogen. Antifungal compound in isolate bacteria ER1I are fenol, lauric acid, propenoic acid, and cyclohexanone.Keywords: Antifungal compound, Endophytic Bacteria, Fungal pathogen

Usaha Pemanfaatan Lahan Pekarangan Budidaya Tanaman Sayuran Secara Vertikultur Pada Kelompok Ibu-Ibu ‘Aisyiyah Juita Rahmadani Manik; Muhammad Alqamari; Andini Hanif
JURNAL PRODIKMAS Hasil Pengabdian Kepada Masyarakat Vol 3, No 1 (2018)
Publisher : Universitas Muhammadiyah Sumatera Utara

The yard in Medan Denai Subdistrict is actually still quite extensive, but most of it is not used for vegetable crops so it is not possible to plant other crops such as vegetables. The main problems that occur in the target community are 1) there are not many activities of the Aisyiyah mothers group related to community empowerment, 2) the yard environment in Medan Denai District is largely untapped for vegetable crops, 3) most members of Aisyiyah do not have knowledge , insight and skills in intensifying the land area to be more productive and provide added value to the community, and 4) the potential of human and natural resources in Medan Denai Subdistrict has not been utilized optimally. To achieve the stated objectives, several methods are used, namely: 1) counseling, which is conducted by gathering organizational leaders and pilgrims to participate in counseling on women's empowerment, managing land and verticulture cultivation technology, with resource persons from the Implementation Team, 2) technology transfer by procuring verticultural installations with various models, as well as preparing other facilities that support this activity, 3) demonstration and training carried out by simulating verticulture cultivation practices using equipment and materials prepared by program implementers, 4) verticulture cultivation practices to produce products , and 5) periodic accompaniment to foster and assist partners until they successfully carry out verticultural cultivation until they achieve optimal results. The results of the Kemuhammadiyaan Coaching program show that the Coaching program provides great benefits for program participants because it can increase the insight, knowledge and skills in using the land so that it becomes more productive.

Effectiveness Test of Tagetes erecta L Powder and Cattle Urine Waste that Potentially Repelent ont Boring Pests of Nezara viridula Pods in Soybean (Glycine max L) in Patumbak Subdistrict, Deli Serdang, North Sumatra Rini Susanti; Andini Hanif; Lisdayani
Jurnal Pertanian Tropik Vol. 6 No. 2 (2019): JURNAL PERTANIAN TROPIK
Publisher : Talenta Publisher

Soybean plants (Glycine max) are the third most important food commodity crop after rice and corn. Soybean production continues to be improved in meeting community needs, but efforts to increase soybean production still face problems, namely green ladybird pest (N. viridula L.). One alternative to control soybean pod sucking insects that are relatively safe, inexpensive, and easily obtained is the use of vegetable insecticides. Vegetable insecticides do not quickly cause pest resistance, are synergistic, and their use can be combined with other pest control techniques. One of them is by using Tagetes erecta plants combined with cow urine as a vegetable insecticide. The use of repellents generally does not directly kill insects, but rather serves to reject the presence of insects, mainly due to the pungent smell. The use of cow urine as a mixture of biopesticides contains growth stimulants and contains repellents for several types of insect pests. This study aims to determine the effectiveness of Tagetes erecta in reducing the population of N.viridula pests in soybean plants (Glycine max L.Merr). Non Factorial RAK with 5 levels of treatment and 5 replications. Hopefully with this study farmers can take advantage of tagetes erecta and cow urine waste that can be used as natural pesticides and can be used as a control for Hama Nezara viridula which is environmentally friendly and economic.

Inventarisasi Dan Identifikasi Cendawan Patogen Terbawa Benih Jagung (Zea Mays L.) Lokal Asal Sumatera Utara Dengan Metode Blotter Test Andini Hanif; Rini Susanti
Jurnal Pertanian Tropik Vol. 6 No. 2 (2019): JURNAL PERTANIAN TROPIK
Publisher : Talenta Publisher

Salah satu faktor utama penyebab penurunan produksi jagung adanya serangan OPT (Organisme Penganggu Tanaman) baik itu hama maupun penyakit yang disebabkan oleh mikrooragnisme terutama cendawan patogen. Cendawan patogen dapat menginfeksi langsung tanaman jagung ataupun menginfeksi benih jagung. Cendawan patogen yang terbawa pada benih dapat mengubah bentuk dan warna benih, hilangnya daya kecambah dan vigor benih, selain itu cendawan patogen yang terbawa pada benih yang tumbuh berpotensi menyebabkan penyakit pada saat perkecambahan atau tanaman dewasa, sehingga tanaman menjadi sakit dan mengurangi hasil produksi. Penelitian ini menggunakan metode standar ISTA (International Seed Testing Association). Isolasi cendawan patogen terbawa benih dilakukan dengan metode blotter test yaitu menginkubasi benih di atas kertas saring lembab, selanjutnya cendawan patogen yang muncul didentifikasi. Cendawan patogen yang diperoleh diuji patogenisitasnya terhadap benih jagung. Hasil deteksi dan identifikasi cendawan pathogen terbawa benih jagung lokal diperoleh cendawan Fusarium sp. yang paling tinggi persentasenya menginfeksi benih jagung varietas lokal asal Sumatera Utara yaitu sebesar 78,75% untuk benih jagung varietas lokal asal Deli serdang, 14,5% untuk benih jagung varietas lokal asal Langkat dan 59,5% untuk benih jagung varietas lokal asal Karo.

Peran Solarisasi Tanah terhadap Pertumbuhan Patogen Tular Tanah dan Populasi Mikroba Tanah Evan Purnama Ramdan; Astri Afriani; Andini Hanif; Cheppy Wati; Nurholis Nurholis; Dwi Astuti; Widodo Widodo
Agrotechnology Research Journal Vol 6, No 1 (2022): Agrotechnology Research Journal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Soil-borne soil pathogens are pathogens that inhabit the soil and can survive for years in the soil, making them very difficult to control. Control with pesticides and fungicides harms the ecosystem, so other controls are needed such as soil solarization. This study aims to determine the growth response of soil-borne pathogens and soil microbial populations to soil solarization treatment. The study used a completely randomized design with 4 treatments consisting of solarization on soil media, solarization on soil and compost media, without solarization on soil media, and without solarization on soil media and compost on plastic trays. Each treatment was repeated 4 times. The soil-borne pathogens used were Sclerotium rolfsii and Rigidoporus lignisus. Each pathogen was planted in each planting medium with a depth of 5 and 10 cm. Then each tray is covered with 0.1 mm thick transparent plastic. Then given solarization treatment for four weeks. At the end of the observation, sclerotia and R. lignosus were grown on PDA media to be tested for pathogen survival and the solarization efficacy against pathogen growth was calculated. Soil samples from each treatment were also taken to calculate the soil microbial population. The results showed that soil solarization was able to suppress the growth of R. lignosus by 80-100% and S. rolfsii by 100%. Meanwhile, the microbes found in the soil solarization treatment consisted of groups of bacteria and fungi, respectively 7.67×104–1.90×107 CFU.mL-1 and 1.00×104–5.82×105 CFU.mL-1.

Karakterisasi Morfologi Cendawan Penyebab Penyakit Busuk Pangkal Batang Pada Bawang Merah (Allium cepa) Andini Hanif; Zamriyetti Zamriyetti
AGRIUM: Jurnal Ilmu Pertanian Vol 26, No 1 (2023)
Publisher : UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/agrium.v26i1.13430

Produksi bawang merah di Sumatera Utara belum mampu mencukupi kebutuhan masyarakat akan bawang merah di Sumatera Utara. Penyakit busuk pangkal batang fusarium (FBR) atau penyakit busuk pangkal batang pada bawang merah merupakan salah satu kendala budidaya bawang merah, dan salah satu penyakit utama pada bawang merah F.oxysporum. F. oxyporum merupakan patogen tular tanah dan juga berpotensi sebagai penyakit tular benih pada bawang merah. Penelitian dilakukan di Desa Pengambaten, Kecamatan Merek, Kabupaten Karo dan di Laboratorium Pertanian Universitas Muhammadiyah Sumatera Utara. Sampel tanaman bawang merah bergejala di ambil pada masing-masing lokasi pengamatan, selanjutnya di bawa ke laboratorium, untuk di isolasi pada media PDA dan dikarakterisasi. Tujuan dari penelitian ini adalah untuk mengetahui penyebab penyakit moler pada bawang merah di desa Pengambaten Kecamatan Merek Kabupaten Karo, dan karakterisasi cendawan patogen penyebab penyakit busuk pangkal batang pada bawang merah. Berdasarkan hasil karakterisasi morfologi makroskopis dan mikrosopis isolat cendawan patogen yang diperoleh adalah Fusarium sp.

Deteksi Fusarium Patogenik Terbawa Umbi Benih Beberapa Varietas Bawang Merah (Allium cepa L. group aggregatum) Andini Hanif; Suryo Wiyono; Abdul Munif; Sri Hendrastuti Hidayat
Agro Bali : Agricultural Journal Vol 7, No 1 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i1.1567

Infeksi patogen tanaman merupakan salah satu penyebab menurunnya produksi bawang merah di Indonesia. Salah satu penyakit utama pada bawang merah adalah busuk pangkal batang yang disebabkan oleh infeksi Fusarium oxysporum. Infeksi Fusarium patogenik pada umbi benih bawang merah dapat menyebabkan kejadian penyakit busuk pangkal batang pada tanaman bawang merah. Oleh karena itu perlu dilakukan uji kesehatan umbi benih, untuk mendeteksi keberadaan Fusarium patogenik pada umbi benih bawang merah. Uji kesehatan umbi benih bawang merah dilakukan dengan menggunakan metode blotter test. Selanjutnya isolat Fusarium yang muncul dari hasil blotter test diuji sifat patogenisitasnya pada umbi dan tanaman bawang merah. Penelitian ini bertujuan untuk mendeteksi keberadaan Fusarium terbawa umbi benih bawang merah pada beberapa varietas dan sifat patogenisitasnya. Hasil penelitian menunjukkan varietas bawang merah dengan persen infeksi Fusarium tertinggi pada varietas Bima (74,5%) dengan nekrosis basal plate (79%). Hasil uji patogenisitas menunjukkan dari 60 isolat Fusarium diperoleh dari uji blotter test, 46,6% merupakan isolat Fusarium patogenik.

Co-Authors Abdul Munif Afriani, Astri Alqamari, Muhammad Bonny Poernomo Wahyu Soekarno Cheppy Wati Dwi Astuti Juita Rahmadani Manik Lisdayani Lisdayani Lisdayani Nurholis Nurholis Ramdan, Evan Purnama Rini Susanti Rini Susanti SRI HENDRASTUTI HIDAYAT Suryo Wiyono Widodo Widodo Wizni Fadhillah Zamriyetti Zamriyetti

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