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All Journal Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Berkala Ilmiah Biologi
BUDI SETIADI DARYONO
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Characterization and Pathogenicity of Fusarium oxysporum as the Causal Agent of Fusarium Wilt in Chili (Capsicum annuum L.) REJEKI SITI FERNIAH; BUDI SETIADI DARYONO; RINA SRI KASIAMDARI; ACHMADI PRIYATMOJO
Microbiology Indonesia Vol. 8 No. 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.32 KB) | DOI: 10.5454/mi.8.3.5

Abstract

Fusarium wilt is a serious disease attacking chili plants in Central Java which cause lost of chili productivity. Fusarium wilt is caused by pathogenic fungi Fusarium oxysporum, which is host specific. The objectives of this research were to characterize the pathogenic F. oxysporum as the causal agent of fusarium wilt in chili plants and to observe the virulence of the pathogen. Fungal pathogen was isolated from Tawangmangu as an endemic area of fusarium wilt in Central Java. The fungi was characterized morphologically and identified molecularly by its internal transcribed spacer regions (ITS regions). Pathogenicity test was done to observe the virulence of the pathogen. One pathogenic strain was isolated from Tawangmangu, Karanganyar and was identified  morphologically and molecularly as F. oxysporum.  
Characteristic Phenotype and Growth of Chicken Layer Strain (Gallus gallus domesticus, Linnaeus 1758) Feren Putri Sholiha; Galy Hardyta; Budi Setiadi Daryono
Berkala Ilmiah Biologi Vol 13 No 1 (2022): Jurnal Berkala Ilmiah Biologi
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v13i1.4329

Abstract

The aim of this research is to study the phenotypic character and growth of layered chickens. In this study, crosses between G4 Layer chickens were carried out which resulted in G5 Layer chickens. Morphological characters, growth weights and measuring the feed-conversion ratio (FCR) were observed. The results obtained were 10 individuals of G5 Layer chickens with the proportion of male gametes (70%) and female gametes (30%). Phenotypic characters are brown fur color and a combination of brown and white. The value of Feed Conversation Ratio (FCR) is 1.67 and the average weight growth of G5 Layer chickens reaches 491.2 grams at the age of 7 weeks.
MOLECULAR ANALYSIS OF WAXY GENE MARKERS IN SORGHUM CROSSES KD4 AND BONTEB GUNUNGKIDUL Muazam, Arif; Kristamtini, Kristamtini; Widyayanti, Setyorini; Nugraha, Yudhistira; Sri Kasiamdari, Rina; Budi Setiadi Daryono
BIOTROPIA Vol. 32 No. 3 (2025): BIOTROPIA Vol. 32 No. 3 December 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.3.2457

Abstract

HIGHLIGHTS- Local sorghum crosses show unique fixation of the waxy starch gene allele- Only Wxc allele is expressed, while other waxy alleles are not detected- Waxy allele expression strongly relates to low amylose grain quality- Marker-based selection supports breeding of soft-textured sorghum- Findings enhance sorghum use for food, feed, and industrial applicationsABSTRACTSorghum (Sorghum bicolor L.) is a food crop exhibiting resilience to extreme environmental conditions and potential to develop as an alternative food source. Quality of sorghum seeds is significantly influenced by starch composition in endosperm regulated by waxy (Wx) gene. This gene has several major alleles: Wxa, Wxb, and Wxc, playing roles in amylopectin and amylose syntheses. The purpose of study is to analyze the expression of Wx alleles in the crossbreeding of sorghum cultivars KD4 and Bonteb Gunungkidul. Main method used was molecular marker-based PCR method. Leaf and seed samples of sorghum were collected and extracted using the CTAB method to obtain high-quality genomic DNA. PCR reactions were performed with specific primers for each allele, and the amplification results were analyzed through 1.5% agarose gel electrophoresis. Statistical analysis was performed to ensure result significance through: Chi-Square Test to determine relationships between waxy allele expression with genetic segregation within cross populations, Allele Frequency Analysis to determine distribution of waxy genotypes within populations by comparing counts showing expressions of Wxa, Wxb, and Wxc, and Pearson Correlation Test to evaluate relationships between waxy gene expression with specific agronomic traits (e.g., amylose content). Main findings showed that only Wxc allele did exhibit a clear amplification band, while Wxa and Wxb did not show significant expression. Further studies are needed to understand the regulation of Wx gene expression and its potential implications to molecular selection, ultimately enhancing sorghum quality for both food and industrial applications.
Community Structure and Genetic Diversity of Dominant Riparian Vegetation Species in the Kedung Ingas River Following Sand Mining Activities Budi Setiadi Daryono; Abdul Razaq Chasani; Nidianti, Esty
Berkala Ilmiah Biologi Vol 17 No 1 (2026)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v17i1.28661

Abstract

Riparian ecosystems play a crucial role in maintaining river ecological functions but are highly vulnerable to anthropogenic disturbances, including sand mining. Sand mining activities in the Kedung Ingas River, Cilegon City, have altered habitat conditions and riparian vegetation structure. This study aimed to evaluate the structure, composition, and genetic diversity of dominant riparian vegetation species following sand mining activities using an integrated ecological and molecular approach based on Inter Simple Sequence Repeat (ISSR) markers. The study was conducted across three riparian zones representing a disturbance gradient: upstream (non-mining), middle (post-sand mining), and downstream (deposition zone). In each zone (±100 m), two observation sites were established (six sites in total), and three plots were set up at each site: 5 m x 5 m (trees), 2 m x 2 m (saplings), and 1 m x 1 m (seedlings). Vegetation structure and composition were analyzed using the Important Value Index (IVI), while community diversity was assessed using the Shannon–Wiener, Simpson, and evenness indices. Seedling community patterns were further examined using Non-metric Multidimensional Scaling (NMDS). Genetic analysis was performed on three dominant species, Swietenia macrophylla, Microcos paniculata, and Musa sp., using five ISSR primers. The results showed that riparian vegetation comprised 28 species from 19 families, with the highest diversity observed in the seedling stratum. The post-mining zone was dominated by pioneer species, whereas the downstream zone indicated secondary succession. ISSR analysis generated a total of 29 loci, with PIC values ranging from 0.26 to 0.44 and polymorphic loci ranging from 60% to 100%, indicating moderate to high levels of genetic diversity. These findings suggest that dominant riparian vegetation still retains sufficient genetic diversity to support natural regeneration and ecosystem restoration following sand mining.
Co-Authors Abdul Razaq Chasani Achmadi Priyatmojo Feren Putri Sholiha Hardyta, G. Kristamtini Kristamtini, Kristamtini Muazam, Arif Nidianti, Esty Rejeki Siti Ferniah Rina Sri Kasiamdari Setyorini Widyayanti, Setyorini Yudhistira Nugraha