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All Journal Microbiology Indonesia Berkala Ilmiah Biologi
BUDI SETIADI DARYONO
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Characterization and Pathogenicity of Fusarium oxysporum as the Causal Agent of Fusarium Wilt in Chili (Capsicum annuum L.) REJEKI SITI FERNIAH; BUDI SETIADI DARYONO; RINA SRI KASIAMDARI; ACHMADI PRIYATMOJO
Microbiology Indonesia Vol. 8 No. 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.32 KB) | DOI: 10.5454/mi.8.3.5

Abstract

Fusarium wilt is a serious disease attacking chili plants in Central Java which cause lost of chili productivity. Fusarium wilt is caused by pathogenic fungi Fusarium oxysporum, which is host specific. The objectives of this research were to characterize the pathogenic F. oxysporum as the causal agent of fusarium wilt in chili plants and to observe the virulence of the pathogen. Fungal pathogen was isolated from Tawangmangu as an endemic area of fusarium wilt in Central Java. The fungi was characterized morphologically and identified molecularly by its internal transcribed spacer regions (ITS regions). Pathogenicity test was done to observe the virulence of the pathogen. One pathogenic strain was isolated from Tawangmangu, Karanganyar and was identified  morphologically and molecularly as F. oxysporum.  
Characteristic Phenotype and Growth of Chicken Layer Strain (Gallus gallus domesticus, Linnaeus 1758) Feren Putri Sholiha; Galy Hardyta; Budi Setiadi Daryono
Berkala Ilmiah Biologi Vol 13 No 1 (2022): Jurnal Berkala Ilmiah Biologi
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v13i1.4329

Abstract

The aim of this research is to study the phenotypic character and growth of layered chickens. In this study, crosses between G4 Layer chickens were carried out which resulted in G5 Layer chickens. Morphological characters, growth weights and measuring the feed-conversion ratio (FCR) were observed. The results obtained were 10 individuals of G5 Layer chickens with the proportion of male gametes (70%) and female gametes (30%). Phenotypic characters are brown fur color and a combination of brown and white. The value of Feed Conversation Ratio (FCR) is 1.67 and the average weight growth of G5 Layer chickens reaches 491.2 grams at the age of 7 weeks.
Community Structure and Genetic Diversity of Dominant Riparian Vegetation Species in the Kedung Ingas River Following Sand Mining Activities Budi Setiadi Daryono; Abdul Razaq Chasani; Nidianti, Esty
Berkala Ilmiah Biologi Vol 17 No 1 (2026)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v17i1.28661

Abstract

Riparian ecosystems play a crucial role in maintaining river ecological functions but are highly vulnerable to anthropogenic disturbances, including sand mining. Sand mining activities in the Kedung Ingas River, Cilegon City, have altered habitat conditions and riparian vegetation structure. This study aimed to evaluate the structure, composition, and genetic diversity of dominant riparian vegetation species following sand mining activities using an integrated ecological and molecular approach based on Inter Simple Sequence Repeat (ISSR) markers. The study was conducted across three riparian zones representing a disturbance gradient: upstream (non-mining), middle (post-sand mining), and downstream (deposition zone). In each zone (±100 m), two observation sites were established (six sites in total), and three plots were set up at each site: 5 m x 5 m (trees), 2 m x 2 m (saplings), and 1 m x 1 m (seedlings). Vegetation structure and composition were analyzed using the Important Value Index (IVI), while community diversity was assessed using the Shannon–Wiener, Simpson, and evenness indices. Seedling community patterns were further examined using Non-metric Multidimensional Scaling (NMDS). Genetic analysis was performed on three dominant species, Swietenia macrophylla, Microcos paniculata, and Musa sp., using five ISSR primers. The results showed that riparian vegetation comprised 28 species from 19 families, with the highest diversity observed in the seedling stratum. The post-mining zone was dominated by pioneer species, whereas the downstream zone indicated secondary succession. ISSR analysis generated a total of 29 loci, with PIC values ranging from 0.26 to 0.44 and polymorphic loci ranging from 60% to 100%, indicating moderate to high levels of genetic diversity. These findings suggest that dominant riparian vegetation still retains sufficient genetic diversity to support natural regeneration and ecosystem restoration following sand mining.
Impact of Fermented Coffee Pulp Inclusion on Growth and cGH Gene Expression in F6 Golden Kamper Hybrid Chickens: English Patricia Graciella Audrey Prajaningtyas; Adib Fakhruddin Yusuf; Yusuf Febrianta; Prananda Imammuddin Dzaki; Budi Setiadi Daryono
Berkala Ilmiah Biologi Vol 17 No 2 (2026)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v7i2.19147

Abstract

Chicken is one of the most sought-after livestock commodities in Indonesia. However, it hasn’t been optimally implemented due to the high price of feed and chicken stocks that still depend on imports. One solution is to find an alternative feed (coffee husk) and cultivate hybrid chickens, for example Golden Kamper from Gama Ayam Research Team. Coffee is a popular trading commodity in Indonesia. However, the use of coffee husk is still not optimal. Coffee husk contains crude protein in the range of 11% and will increase if fermented. Therefore, coffee husk has the potential to be an alternative feed for chickens. The purpose of this study was to determine the effect of fermented coffee husk feed on body weight and cGH (Chicken Growth Hormone) gene expression in F6 Golden Kamper chickens and to determine the effectiveness and potential of coffee husk as an alternative feed for chickens. The study was conducted by making coffee skin fermentation with EM4, making feed composition, raising DOC at the starter point (three weeks old), collecting chicken weight data, and analyzing of gene expression. The results showed that the fermentation process was able to reduce the crude fiber content in coffee husk by up to 12%. Feeding with 10% coffee husk gave better results than the 20%​. Meanwhile, the results of body weight and carcass in chickens were not significantly different in the two treatments. Gene expression analysis showed that feeding with 10% coffee husk treatment resulted in a gene expression level twice as high as 20%. The correlation test showed no correlation between chicken weight and cGH gene expression levels.
Co-Authors Abdul Razaq Chasani Achmadi Priyatmojo Adib Fakhruddin Yusuf Feren Putri Sholiha Hardyta, G. Nidianti, Esty Patricia Graciella Audrey Prajaningtyas Prananda Imammuddin Dzaki Rejeki Siti Ferniah Rina Sri Kasiamdari Yusuf Febrianta