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UJI SITOTOKSIK DENGAN METODE BRINE SHRIMP LETHALITY TEST DAN PROFIL METABOLIT SEKUNDER DARI EKSTRAK BIJI BUAH BERENUK (Crescentia cujete Linn) Afdhil Arel
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 15 No 2 Desember 2018
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169 KB) | DOI: 10.31942/jiffk.v15i2.2559

Abstract

ABSTRACTThe purpose of this research is to find out the profile of secondary metabolite contained in the extract of seeds (Crescentia cujete Linn.) And cytotoxic test against shrimp larvae Artemia salina Leach by BSLT method. Phytochemical tests showed that the extract be expected contained the terpenoid, alkaloid, phenolic, secondary metabolite compounds. The results of qualitative analysis with positive TLC contains phenolics. The result of identification with Spectrophotometer UV-visfor determination of α max from berenuk seeds extract consisted of 3 peaks with α max 326 nm, 258 nm, 222 nm be expected terpenoid compound, alkaloid, and phenolic. The results of toxicity test using shrimp larvae (Artemia salina leach) were divided into 4 groups: 1 control group and 3 groups of concentration series. The concentration of test solution used was 1,000, 100 and 10 μg / ml. The shrimp larvae mortality was observed after 24 hours of treatment. Based on the analysis with probit method, LC50 value of seed extract berenuk to shrimp larvae Artemia salina Leach is equal to 82,30 μg/ml. This shows that the extract of berenuk berries is toxic to shrimp larvae Artemia salina Leach, presence to terpenoid content, alkaloids and phenolics in the extract of seeds berenuk.Keywords : Secondary metabolite; Cytotoxic; BSLT.
PENETAPAN KADAR KOFEIN DALAM MINUMAN BERNERGI YANG BEREDAR DI PASARAN DENGAN METODE KROMATOGRAFI CAIR KINERJA TINGGI Afdhil Arel; B.A. Martinus; Romi Nofiandri
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 14 No 1 juni 2017
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (67.1 KB) | DOI: 10.31942/jiffk.v14i1.1767

Abstract

INTISARI                            Telah dilakukan penelitian tentang penetapan kadar kofein dalam minuman berenergi yang beredar dipasaran secara Kromatografi Cair Kinerja Tinggi. Minuman energi ini difraksinasi dengan kloroform. Hasil fraksi kloroform diuapkan dengan  rotary  evaporator. Kadar kofein diukur dengan kromatografi cair kinerja tinggi menggunakan kolom LC- 20AD dengan panjang 25 cm dan detektor SPD-20MAD dengan panjang gelombang 272nm. Fase gerak yang digunakan Metanol : Air (50:50)dengan laju alir 1 mL/menit. Dari hasil penelitian didapatkan kadar kofein dalam 1 botol sampel Kr =27,73 mg,  M =34,14 mg, , Pm=28,41 mg dan sampel Lv =69,78 mg. Hasil tersebut sampel Lv yang melebihi batas yang telah ditentukan oleh SNI No. 01-6684-2002 yaitu kurang dari 50 mg persaji. Kata Kunci : KCKT (Kromatografi cair kinerja tinggi, Koffein  ABSTRACT This research is telling about estimating amount of coffeine cosumption in energy drink which available in the market with HPLC method. Energy drink filtrat fractionated with chloroform  and chloroforom fractionation evaporated by rotary evaporator. Coffein amount measured by HPLC using column LC- 20 AD ( Length 20 cm ) and detector SPD – 20 MAD (λ 272 nm) with mobile phase methanol : water (50:50).  Amount  estimating  of  coffein  from  this  research  in  1  bottle  Sample Kr =27,7264 mg, M =34,140 mg, Pm=28,41 mg and sample Lv= 69,78 mg. The results sample Lv that exceed the limit set by SNI Num. 01-6684-2002 is less than 50 mg. Keywords: HPLC(high performance liquid chromatography), Coffeine
PENENTUAN PENGURANGAN KADAR FORMALIN PADA IKAN ASIN SEPAT DENGAN PERENDAMAN PERASAN BELIMBING WULUH DAN VARIASI SUHU AKUADES Afdhil Arel; B.A. Martinus; Halimatun Sya’diyah
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 14 No 2 Desember 2017
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.377 KB) | DOI: 10.31942/jiffk.v14i2.2065

Abstract

ABSTRACT This research was conducted with the aim to determine the effect of  soaking  with starfruit (Averrhoa bilimbi L) and the effect of soaking with variations in water temperature to reducing levels of formaldehyde the salted fish. Research using Spektroquant NOVA 400 on wavelength 565 nm and using reagent HCHO-1 and HCHO-2   to  know  levels  of  formaldehyde  in  the  salted  fish.  The  results   showedpercentage levels of formaldehydewithout soaking is 0%, while soaking with cold  water (28ºC  - 29ºC) is 31,62%, soaking with warm water (50ºC  -  60ºC) is 53,69%, soaking with hot water (98ºC  -  99ºC) is 62,78%, while soaking with starfruit konsentration 100% is 94,47%. This shows the soaking with water can reduce levels of formaldehyde highest with hot water, but soaking with starfruit konsentration 100% can reduce levels of formaldehyde higher of the soaking with hot water. Statistical calculation results with one-way ANOVA (p<0,05) gives results significantly  differentbetween each treatment. Keywords : Formaldehyde, Starfruit (Averrhoa bilimbi L), Salted Fish, Spektroquan NOVA 400
ANALISIS MERKURI (Hg) PADA LOTION PEMUTIH YANG BEREDAR DI PASAR RAYA KOTA PADANG MENGGUNAKAN SPEKTROFOTOMETER SERAPAN ATOM Afdhil Arel; Regina Andayani; Aulia Rahmi; Wida Ningsih
Jurnal Ilmu Farmasi dan Farmasi Klinik Vol 16, No 02 (2019): Jurnal Ilmu Farmasi & farmasi Klinik Vol 16 No 02 Desember 2019
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.94 KB) | DOI: 10.31942/jiffk.v16i02.3238

Abstract

ABSTRACT Whitening lotion is a mixture of chemicals or other ingredients with properties that can whiten the skin or blemish black (brown) on the skin. The use of mercury in whitening lotions can cause a variety of things, ranging from skin discoloration which can ultimately cause black spots on the skin, allergies and skin irritation. The using of mercury in high dose can cause permanent brain damageand cancer. An analysis of mercury content in the whitening lotion brands A, B and C circulating at market in Padang city has been conducted. Extraction of mercury in the whitening lotion was done by wet destruction with a concentrated HNO3 solution and concentrated H2SO4 for 2 hours at a temperature of ± 95°C until a clear solution is obtained. The clear solution was measured by absorption with an Atomic Absorption Spectrophotometer using a mercury hollow cathode lamp at a wavelength of 253.7 nm. The results of this study were samples of brands A, B and C detected containing mercury metals with successive levels of 0.3347 μg /g; 0,7917 μg /g and 0,7981 μg/ g. The whitening lotions that circulate in market do not meet the Head of BPOM RI Regulation No.18 of 2015 whose contents prohibit the use of mercury in cosmetics preparations. Keywords: mercury, whitening lotion, Atomic Absorption Spectrophotometer
PENGEMBANGAN USAHATANI GAMBIR MELALUI PRODUK TANIN Wedy Nasrul; Dedi Satria; Rudi Kurniawan Arief; Afdhil Arel; Leli Suwita; Revi Ernanda; Fajri Ramadhan
Jurnal Menara Ekonomi : Penelitian dan Kajian Ilmiah Bidang Ekonomi Vol 9, No 2 (2023): VOLUME IX NO. 2 Oktober 2023
Publisher : Jurnal Menara Ekonomi : Pelatihan dan Kajian Ilmiah Bidang Ekonomi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31869/me.v9i2.4856

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Usahatani gambir menjadi mata pencarian pokok petani di wilayah sentra gambir. Produksi gambir saat ini fokus pada produk gambir biasa (lumpang). Produk yang dihasilkan saat ini memiliki mutu yang buruk dan harga yang rendah. Sehingga perlu pengembangan produk gambir untuk mendapatkan harga yang lebih baik dan tambahan penghasilan. Pengembangan usaha gambir penting agar petani tidak tergantung pada satu produk dan pasar. Penelitan ini menggunakan metode kulitatif dan kuantitatif. Metode kualitatif untuk menjelaskan pengembangan usahatani gambir berbasis tanin. Metode kuantitatif untuk menganalisis kelayakan pengembangan usaha gambir. Hasil penelitian menunjukan produksi gambir dapat dikembangkan dengan alat-alat sederhana dengan biaya terjangkau. Tanin memiliki harga yang lebih mahal dibanding harga gambir biasa. Harga gambir biasa sekitar Rp. 80.000,-/kg sedangkan harga tanin mencapai Rp. 300.000,-/kg. Pengembangan agroindustri tanin layak untuk dikembangkan, dimana hasil analisis Revenue Cost Ratio (R/C) sebesar 1,6. Break Event Point (BEP) untuk katekin Rp. 118.787,-/Kg jadi sangat menguntungkan dilihat dari harga tanin saat ini.Kata Kunci : Gambir, Petani, Tanin
Penyusunan Standar Operasional Prosedur (SOP) Produksi Katekin dan Tanin dari Gambir Wedy Nasrul; Rudi Kurniawan Arief; Afdhil Arel
Jurnal Pengabdian UntukMu NegeRI Vol 8 No 3 (2024): Pengabdian Untuk Mu negeRI
Publisher : LPPM UMRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jpumri.v8i3.7757

Abstract

Standard Operating Procedures (SOP) for production are documents that contain step-by-step instructions to guide workers on how to carry out repetitive production processes for a job to achieve production goals. Production SOP function to guide workers in the production process and maintain the quality of the products produced. The Sharia Producer Cooperative "Gambir Anam Koto Mandiri" which produces catechins and tannins does not yet have a production SOP. So it is important to prepare SOP for catechin production and gambier farming to help workers in the production process and maintain the quality of the products produced. The activity method is in the form of training and mentoring. The results of training activities show that cooperative administrators can prepare SOP for the production of catechins and tannins. The results of the assistance show that cooperative members can produce gambier catechin and tannin in accordance with the SOP. Activities produce catechin and tannin products which are produced to meet specified quality standards