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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Proceeding International Conference on Global Resource Conservation Buletin Kebun Raya Journal of Tropical Biodiversity and Biotechnology
Arief Priyadi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Other

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Selecting Tree Species with High Carbon Stock Potency from Tropical Upland Forest of Bedugul-Bali, Indonesia Priyadi, Arief; Sutomo, Sutomo; Darma, I Dewa Putu; Arinasa, Ida Bagus Ketut
Journal of Tropical Life Science Vol 4, No 3 (2014)
Publisher : Journal of Tropical Life Science

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Selecting Tree Species with High Carbon Stock Potency from Tropical Upland Forest of Bedugul-Bali, Indonesia Arief Priyadi; Sutomo Sutomo; I Dewa Putu Darma; Ida Bagus Ketut Arinasa
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
Publisher : Journal of Tropical Life Science

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Vegetation studies to reveal tree diversity and its contribution to carbon stock were conducted in three differ­ent sites of upland forest in Bali, Indonesia. The sites were located approximately 60 km north of the Bali Province capital city of Denpasar in an area named Bedugul. Those three sites were Mt. Mangu (forest area east of Beratan lake), forest area west of Buyan lake and forest area south of Tamblingan lake. There were 44, 29, and 21 tree species of 14, 19, 14 families with Shannon Diversity Index (H) of 2.87, 2.64 and 1.69 respectively. Carbon stock average of above ground tree biomass from these sites were 214.2, 693.0 and 749.1 ton.ha-1 respectively. Tree species with top Summed Dominance Ratio (SDR) in each of those sites were Platea latifolia in Mt. Mangu, Plan­chonella sp. in Buyan, and Tabernaemontana macrocarpa in Tamblingan. Average carbon content of these three species were 493.25, 12,876.26 and 40.35 kg.ha-1 respectively.
Analysis of trnL- trnL- trnF Intergenic Spacer and matK Sequences Combined with Morphological Observations Showed Pucuk Seminyak from Riau is Champereia manillana var. manillana Merr.: trnL-trnL-trnF Intergenic Spacer and matK on Pucuk Seminyak Roslim, Dewi Indriyani; Herman; Budiono, Deanne Yoshe Fidela; Cahyati, Isa Endar; Lestari, Wahyu; Novita, Linda; Priyadi, Arief
Journal of Tropical Life Science Vol. 14 No. 3 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.13

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A taxonomic status of Pucuk Seminyak plant is still unclear. This plant has a fruit like Melinjo (Gnetum gnemon) fruit and is often classified as a member of Gnetaceae family. This study aims to determine the taxonomic status of Pucuk Seminyak from Riau using trnL-trnL-trnF intergenic spacer (IGS) and matK sequences. Methods included leaf samples from three individuals collected from the Kampar Regency, Riau Province, total DNA extraction, electrophoresis, polymerase chain reaction (PCR), and data analysis. The results showed that the trnL-trnL-trnF IGS DNA sequences from the three samples of Pucuk Seminyak were obtained 937 bp length, while the matK had a size of 775 bp. These sequences have been registered in GenBank with registration numbers OQ174512, OQ174513, and OQ174514 for trnL-trnL-trnF IGS, and OQ174525, OQ174526, and OQ174527 for matK. The highest similarity was found between Pucuk Seminyak and Champereia manillana with an identity value of 99.67% based on trnL-trnL-trnF IGS and 99.74% based on matK. BLASTn analysis by default settings and organism Champereia (taxid: 50116) resulted in hits on the Champereia genus of the Opiliaceae. Further phylogenetic analysis placed the study objects in the Champereia clade (bootstrap support 49 for trnL-trnL-trnF, 68 for matK, and 71 for concatenated sequences). Lastly, identification by morphological characters also confirmed that the specimen agreed with the description and identification key for C. manillana. The species is classified into two varieties: var. longistaminea and var. manillana. The former was distributed in Southwest China, and the latter was distributed in Malesia to India. Furthermore, the phylogenetic analysis separated the two varieties into different clades. Therefore, it can be concluded that our specimen of Pucuk Seminyak is identified as C. manillana var. manillana Merr. (Opiliaceae).
Co-Authors Asih, Ni Putu Sri Budiono, Deanne Yoshe Fidela Cahyati, Isa Endar Dewi Indriyani Roslim Evy Yulianti Herman I Dewa Putu Darma I Dewa Putu Darma I Nyoman Lugrayasa I Nyoman Peneng I Putu Agus Hendra Wibawa Ida Bagus Ketut Arinasa Ida Bagus Ketut Arinasa Linda Novita Paramita Cahyaningrum Kuswandi Pratiwi, Risha Amilia Sutomo Sutomo Sutomo Sutomo Wahyu Lestari Wenni Setyo Lestari, Wenni Setyo