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All Journal Indonesian Green Technology Journal The Journal of Pure and Applied Chemistry Research
Sasangka Prasetyawan
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Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Engineering Materials Science & Nanotechnology Medicine & Pharmacology Other

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Production and Characterization of Bacillus firmus pectinase Anna Roosdiana; Sasangka Prasetyawan; Chanif Mahdi; Sutrisno Sutrisno
The Journal of Pure and Applied Chemistry Research Vol 2, No 1 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.693 KB) | DOI: 10.21776/ub.jpacr.2013.002.01.111

Abstract

Pectinase is enzyme which functions to hydrolyze pectin become D-galacturonic acid unit. This enzyme is potential in various industries, especially in fruit juice industry.  Pectinase can be derived from various microorganisms resulting in different pectinase character. The aims of this research were to determine the optimum condition of pectinase production and to characterize the resulted pectinase including optimum condition of pectinase activity and the influence of metal ion.  The optimum condition of pectinase production was carried out by growing Bacillus firmus on basal media containing pectin as inducer at various  pH (5, 6, 7, 8, 9, 10), temperature (30, 35, 40, 45, 50) oC and fermentation time (6, 12, 18, 24, 30, 36) hours. while the optimum pectinase activity was done at various pH ( 4, 6, 7, 8, 10 ), temperature (30, 35, 40, 45, 50) oC and reaction time (10, 20, 30, 40, 50) minutes. The influence of Zn2+, Mg2+, K+ at 2-10 mM to pectinase activity were also investigated. The result showed that optimum condition of pectinase production occurred at pH7-8, temperature 40-50 oC and fermentation time 18hours, while the optimum condition of pectinase activity was pH 7, temperature 50 oC and reaction time 30 minutes. The existence of Zn2+, Mg2+, K+ ions  affected significantly to pectinase activity.  Mg2+ acted as non competitive inhibitor; however K+ and Zn2+ acted as un competitive inhibitor.
Effect of Eco-Enzyme’s Storage Duration on Hydrogen Peroxide Level and Its Antibacterial Activity Primarista, Ni Putu; Imam Abu Hanifah; Arie Srihadyastutie; Sasangka Prasetyawan; Ulfa Andayani; Tri Adyati
Indonesian Green Technology Journal Vol. 12 No. 1 (2023)
Publisher : Sekolah Pascasarjana, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.igtj.2023.012.01.03

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Eco-enzyme is a filtrate produced by fermenting sugar and a mixture of organic matter for 3 months. Eco-enzymes have been widely used as cleaning fluids and disinfectants. This study aims to investigate the levels of hydrogen peroxide and the antibacterial activity of eco-enzymes stored at different times. Hydrogen peroxide level was analyzed by redox titration and the antibacterial activity against Escherichia coli and Staphylococcus aureus was analyzed by paper disc method. This study shows that there were significant differences in the levels of hydrogen peroxide and the antibacterial activity of the eco-enzymes stored for a certain time. Hydrogen peroxide levels increased from 0 months of storage to reach the highest point at 10 months of storage with 2664.9 ppm and then it began to decrease to the lowest point at 114 months of storage. The highest antibacterial activity against Escherichia coli and Staphylococcus aureus was shown by eco-enzyme stored for 0 months, with inhibitory zones of 6.37 mm and 6.59 mm, respectively. Eco-enzyme no longer showed antibacterial activity after 15 months of storage. Overall, the storage duration of the eco-enzyme affects the hydrogen peroxide level and antibacterial activity against Escherichia coli and Staphylococcus aureus due to the amount of lactic acid bacteria in the eco-enzyme. Keywords: antibacterial activity, eco enzyme, hydrogen peroxide, storage duration
Co-Authors Anna Roosdiana Arie Srihadyastutie Chanif Mahdi Imam Abu Hanifah Primarista, Ni Putu Sutrisno Sutrisno Tri Adyati Ulfa Andayani